Medical Journal of Cell Biology最新文献_第6页

Properties of coagulase-positive staphylococcal cells that make it difficult to diagnose and treat mastitis in cows 凝固酶阳性葡萄球菌细胞的特性使奶牛乳腺炎的诊断和治疗变得困难

Medical Journal of Cell Biology Pub Date : 2021-09-01 DOI: 10.2478/acb-2021-0014

Arkadiusz Grzeczka, W. Niewitecki

引用次数: 0

The history of melanoma diagnostics 黑素瘤的诊断史

Medical Journal of Cell Biology Pub Date : 2021-09-01 DOI: 10.2478/acb-2021-0018

Paula Kamińska, K. Buszka, M. Nowicki, Joanna Budna-Tukan

引用次数: 0

Mesenchymal stem cells and their secretome - candidates for safe and effective therapy for systemic lupus erythematosus 间充质干细胞及其分泌组——安全有效治疗系统性红斑狼疮的候选细胞

Medical Journal of Cell Biology Pub Date : 2021-09-01 DOI: 10.2478/acb-2021-0016

M. Popis, A. Konwerska, M. Partyka, M. Wieczorkiewicz, S. Ciesiółka, K. Stefańska, Julia Spaczyńska, A. Golkar-Narenji, M. Ješeta, D. Bukowska, P. Mozdziak, M. Dyszkiewicz-Konwińska

{"title":"Mesenchymal stem cells and their secretome - candidates for safe and effective therapy for systemic lupus erythematosus","authors":"M. Popis, A. Konwerska, M. Partyka, M. Wieczorkiewicz, S. Ciesiółka, K. Stefańska, Julia Spaczyńska, A. Golkar-Narenji, M. Ješeta, D. Bukowska, P. Mozdziak, M. Dyszkiewicz-Konwińska","doi":"10.2478/acb-2021-0016","DOIUrl":"https://doi.org/10.2478/acb-2021-0016","url":null,"abstract":"Abstract More than 80 diseases are currently classified as autoimmune, with a rising prevalence throughout the world. Systemic lupus erythematosus (SLE) is classified as a systemic autoimmune disorder, but the exact pathogenesis of SLE remains elusive. Currently available treatment strategies offer only the possibility for disease remission making it essential to develop more effective and safer strategies for treatment. Recently MSCs are gaining attention as attractive therapeutic tools for autoimmune disease treatment. Special focus should be given to MSCs originated from perinatal tissues such as Wharton's jelly, as they present unique immunomodulatory properties and remarkably low immunogenicity. MSCs exert their immunomodulatory effects via direct cell-to-cell communication as well as in a paracrine manner, creating possibility to apply secretome of MSCs as an individual therapeutic tool. Although the secretome of MSCs has not yet been utilized in SLE treatment, its efficacy has been suggested in other disorders, such as multiple sclerosis or Alzheimer's disease. Regular administration of paracrine factors derived from MSCs could potentially effect in significant reduction of SLE symptoms and in maintenance of disease remission.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"9 1","pages":"110 - 122"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48240850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The rostral epidural rete mirabile of the llama as a place of retrograde transport of various substances – anatomical basics 骆驼的吻端硬膜外mirabile网作为各种物质逆行运输的场所——解剖学基础

Medical Journal of Cell Biology Pub Date : 2021-09-01 DOI: 10.2478/acb-2021-0015

M. Zdun, Arkadiusz Grzeczka, M. Zawadzki, H. Frąckowiak

{"title":"The rostral epidural rete mirabile of the llama as a place of retrograde transport of various substances – anatomical basics","authors":"M. Zdun, Arkadiusz Grzeczka, M. Zawadzki, H. Frąckowiak","doi":"10.2478/acb-2021-0015","DOIUrl":"https://doi.org/10.2478/acb-2021-0015","url":null,"abstract":"Abstract The aim of this study was to analyse the structure of the rostral epidural rete mirabile in the llama. Some specimens were prepared by injecting stained chemically cured acrylic into the bilateral common carotid arteries. After about 1 month received vascular corrosion casts on the bone scaffold. Some specimens made using red and blue latex introduced into the bilateral common carotid arteries and the bilateral external jugular vein. The rostral epidural rete mirabile is a well-developed, bilateral structure composed of numerous arteries, which are multiply anastomosed with each other. The cranial section of the rete is asymmetrical. Its lateral part is much better developed, because there are rostral branches to the rostral epidural rete mirabile at this point. The arterial vessels are not accompanied by homonymous veins. However, the arteries of the rostral epidural rete mirabile are accompanied by venous vessels of the cavernous sinus. That rete plays an important role in selective brain cooling, the conservation of body water, and retrograde transport of neurotransmitters. CO, GnRH, beta-endorphin, progesterone, testosterone, oxytocin, LHRH and dopamine diffuse from the venous blood of the cavernous sinus to the arterial blood of the rostral epidural rete mirabile.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"9 1","pages":"105 - 109"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47629088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Effectiveness of embryo transfer in cows - risk factors including in vivo derived and in vitro produced embryos 奶牛胚胎移植的有效性——包括体内胚胎和体外胚胎在内的风险因素

Medical Journal of Cell Biology Pub Date : 2021-09-01 DOI: 10.2478/acb-2021-0017

M. Wieczorkiewicz, J. Jaśkowski, Agnieszka Wichtowska, M. Olszewska-Tomczyk, B. Jaśkowski

引用次数: 0

Recent findings on perinatal mesenchymal stem cells – their possible application in current advanced medicine 围产期间充质干细胞的最新发现及其在当前高级医学中的可能应用

Medical Journal of Cell Biology Pub Date : 2021-06-01 DOI: 10.2478/acb-2021-0008

Rafał Sibiak, K. Stefańska, Kornel Ratajczak, D. Bukowska, P. Antosik, P. Mozdziak, M. Ješeta

{"title":"Recent findings on perinatal mesenchymal stem cells – their possible application in current advanced medicine","authors":"Rafał Sibiak, K. Stefańska, Kornel Ratajczak, D. Bukowska, P. Antosik, P. Mozdziak, M. Ješeta","doi":"10.2478/acb-2021-0008","DOIUrl":"https://doi.org/10.2478/acb-2021-0008","url":null,"abstract":"Abstract The mature human placenta and umbilical cord are rich sources of perinatal mesenchymal stem cells (MSCs). Both cell populations have similar characteristics and cellular properties. Each population can differentiate into multiple mesenchymal subpopulations and retain their self-renewal capacities. Perinatal stem cells can be isolated from tissues obtained from the planned cesarean sections and vaginal deliveries. Their isolation is relatively easy, making them readily available for implementation in various in vitro studies and clinical trials. Their differentiation abilities could be used in advanced regenerative medicine protocols to form new bone, cartilage, or tendons. Moreover, their unique anti-inflammatory and immunomodulatory properties have been implemented in the experimental treatment of multiple autoimmune and degenerative diseases. Numerous phase I/II clinical trials confirmed the safety of perinatal MSCs injections and infusions, albeit the efficacy of those cellular therapies should be investigated in the subsequent large-scale randomized trials. Running title: Clinical applications of the perinatal mesenchymal stem cells","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"9 1","pages":"48 - 55"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49369285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Positive influence of aminosilanes on anti-EpCAM antibody immobilization on a glass surface 氨基硅烷对抗epcam抗体在玻璃表面固定化的积极影响

Medical Journal of Cell Biology Pub Date : 2021-06-01 DOI: 10.2478/acb-2021-0013

Paula Kamińska, K. Buszka, Przemysław Pietras, M. Zabel, M. Nowicki, Joanna Budna-Tukan

{"title":"Positive influence of aminosilanes on anti-EpCAM antibody immobilization on a glass surface","authors":"Paula Kamińska, K. Buszka, Przemysław Pietras, M. Zabel, M. Nowicki, Joanna Budna-Tukan","doi":"10.2478/acb-2021-0013","DOIUrl":"https://doi.org/10.2478/acb-2021-0013","url":null,"abstract":"Abstract Immobilization of antibodies has a number of promising applications, including detection of biomolecules and cells. Well-oriented antibodies are required to bind them effectively. To eliminate the problem of random antibodies’ orientation, the surface of the device can be modified with silanes. This study aimed at elucidating if selected aminosilanes were able to bind antibodies in the appropriate orientation and thus retain their binding activity. Silanization of glass slides was performed using three amino-functional trialkoxysilanes – A, AE, and AEE. The immunofluorescent reaction was used to evaluate the potential of the silanized glass surface to bind anti-EpCAM antibodies. The affinity of selected anti-EpCAM HEA125 antibodies labeled with fluorochrome to tested silanized surfaces was evaluated by measuring the mean fluorescence intensity (MFI) in each analyzed area. The presented silanes effectively bound antibodies. Higher fluorescence intensity was noticed in the case of silane-coated glass slides in comparison to unmodified ones. The differences in the contact angles also confirmed this result. In the case of silane A, the fluorescence intensity reflected the amount of bound antibodies. However, there was no such a relation in the case of the silanes AE and AEE. Although our research gave promising results, the usefulness of selected silanes needs to be confirmed by further studies using cancer cells. Running title: Aminosilanes as enhancers of antibody immobilization","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"9 1","pages":"93 - 99"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46281663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The challenges of treating a patient with recurrent congenital toxoplasmic chorioretinitis 复发性先天性弓形虫性脉络膜视网膜炎患者的治疗挑战

Medical Journal of Cell Biology Pub Date : 2021-06-01 DOI: 10.2478/acb-2021-0009

Aleksandra Krasińska, K. Jaz, J. Mamczur, J. Kocięcki

{"title":"The challenges of treating a patient with recurrent congenital toxoplasmic chorioretinitis","authors":"Aleksandra Krasińska, K. Jaz, J. Mamczur, J. Kocięcki","doi":"10.2478/acb-2021-0009","DOIUrl":"https://doi.org/10.2478/acb-2021-0009","url":null,"abstract":"Abstract Congenital toxoplasmosis is a rare, non-curable parasite infection, that affects approximately 242 children in Europe each year. Poland has one of the highest rates of congenital toxoplasmosis in Europe. Transmission of Toxoplasma gondii to the fetus results in numerous medical conditions, such as developmental delay, intellectual disabilities, seizures, hearing loss, and blindness. Chorioretinitis is a serious manifestation of congenital toxoplasmosis that can recur even after 25 years from the primary infection, which poses a significant therapeutic challenge. A 41-year-old female reported to the Ophthalmology Emergency Room due to blurred vision and pain in the right eye, accompanied by a constant headache. The patient suffered from congenital toxoplasmosis with two relapses in the past. On examination, the best-corrected visual acuity was 1,0 in both eyes, and the intraocular pressure was significantly increased. Slit-lamp examination showed vitritis and an active retinochoroidal lesion in the right eye. In the left eye, there was a retinochoroidal scar. A relapse of toxoplasmosis was suspected. Serology for Toxoplasma gondii was positive. Pyrimethamine with sulfadiazine, clindamycin, topical corticosteroids, and intraocular pressure-lowering drugs were implemented. During the treatment, the patient developed corticonuclear cataract in both eyes and reported psychotic symptoms. Clinical condition improved after the treatment with corticosteroids at a lower dose. Treatment of ocular manifestations of congenital toxoplasmosis is challenging. The clinical benefit of treatment should be weighed against side effects for each patient. Running title: Congenital toxoplasmosis treatment","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"9 1","pages":"56 - 59"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45922862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Nitric oxide may regulate focal adhesion turnover and cell migration in MDA-MB-231 breast cancer cells by modulating early endosome trafficking 一氧化氮可能通过调节早期内皮体运输来调节MDA-MB-231乳腺癌症细胞的局灶性粘附翻转和细胞迁移

Medical Journal of Cell Biology Pub Date : 2021-06-01 DOI: 10.2478/acb-2021-0010

D. Al-Fahad, B. Alharbi, Clementino Ibeas Bih, P. Dash

{"title":"Nitric oxide may regulate focal adhesion turnover and cell migration in MDA-MB-231 breast cancer cells by modulating early endosome trafficking","authors":"D. Al-Fahad, B. Alharbi, Clementino Ibeas Bih, P. Dash","doi":"10.2478/acb-2021-0010","DOIUrl":"https://doi.org/10.2478/acb-2021-0010","url":null,"abstract":"Abstract Cell migration is an essential process for wound healing, metastasis and inflammation. Focal adhesions (FA) are local regions of plasma membrane consisting of multiprotein complexes providing adhesive contact between the cell and the extracellular matrix (ECM). FA turnover regulates different signalling pathways implicated in various cellular responses (e.g. cell migration). Endocytosis, specifically the dynamin and clathrin pathways, is known to regulate cell migration by modulating FA dynamics. In this study, we investigated whether NO activity regulates cell migration, FA dynamics and early endosome trafficking in MDA-MB-231 cells. The assessment of cell migration showed a slowing down of cell migration and an increased duration of FA turnover in cells treated with inhibitors of NO synthase (NOS) such as L-NAME or 1400W. In addition, these treatments were found to exhibit no effect on transferrin and dextran uptake mediated by endocytosis and micropinocytosis, respectively. The number of early endosome antigen 1 (EEA1)-positive endosomes was reduced while their sizes were found to increase in cells treated with L-NAME or 1400W. In contrast, these inhibitors did not affect the number nor the size of Rab5-positive endosomes. Furthermore, we demonstrated that EEA1, endothelial NO synthase (eNOS) and inducible NO synthase (iNOS) were colocalised. Using the biotin switch assay followed by western blot, we showed that early endosome proteins such as APPL1, EEA1, Rab5 were found to be S-nitrosylated. These results were further supported by the sequence analysis performed with the GPS-SNO algorithm which predicted the S-nitrosylation of these endosomal proteins. Taken together, our findings suggest that NO might be involved in cell migration and FA turnover through early endosome trafficking in MDA-MB-231 cells. Running title: Nitric oxide in MDA-MB-231 breast cancer cells","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"9 1","pages":"60 - 72"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49340078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Identification of the respiratory tract infection due to methicillin-resistant Staphylococcus aureus by TaqMan real-time PCR TaqMan实时PCR检测耐甲氧西林金黄色葡萄球菌呼吸道感染

Medical Journal of Cell Biology Pub Date : 2021-06-01 DOI: 10.2478/acb-2021-0012

Sabah Saad Abdulsahib

{"title":"Identification of the respiratory tract infection due to methicillin-resistant Staphylococcus aureus by TaqMan real-time PCR","authors":"Sabah Saad Abdulsahib","doi":"10.2478/acb-2021-0012","DOIUrl":"https://doi.org/10.2478/acb-2021-0012","url":null,"abstract":"Abstract The methicillin-resistant Staphylococcus aureus (MRSA) is a significant human pathogenic bacterium that is endemic within hospitals around the world. The identification and inspection of MRSA in clinical samples is quite helpful both in advising individual patients about the required care and in tracking these species. The goal of this study was to present a modern, faster, and more accurate diagnostic technique to operate on the real-time duplex PCR applicable to S. aureus/MRSA monitoring in Iraqi patients. For this reason, the S. aureus-specific nuc gene sequence and the mecA gene sequence were checked simultaneously. To estimate the assay efficiency, a set of six target strains, 34 non-target strains, and 296 clinical specimens were used. The findings obtained from the diagnosis of a total of 296 isolates based on phenotypic characteristics and biochemical tests showed that 146 (49.32%) were classified as individuals with respiratory tract infections of S. aureus with a total male to female ratio of 1.47, and 142 isolates demonstrated methicillin resistance. 142 MRSA isolates were investigated in the molecular analysis, all MRSA isolates had positive results for the nuc gene and 138 isolates were positive for the mecA gene. The current real-time PCR assay has 97% sensitivity, 100% specificity, and 98% accuracy. Running title: Identification of the MRSA by real time PCR","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"9 1","pages":"86 - 92"},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47366918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0