Medical Journal of Cell Biology最新文献_第5页

Challenges of stem cell application in research and clinical practice – an update 干细胞在研究和临床实践中应用的挑战——最新进展

Medical Journal of Cell Biology Pub Date : 2021-12-01 DOI: 10.2478/acb-2021-0022

M. Jankowski, M. Machatkova, P. Ventruba, E. Kistanova, A. Makarevich, R. Procházka, M. Ješeta

{"title":"Challenges of stem cell application in research and clinical practice – an update","authors":"M. Jankowski, M. Machatkova, P. Ventruba, E. Kistanova, A. Makarevich, R. Procházka, M. Ješeta","doi":"10.2478/acb-2021-0022","DOIUrl":"https://doi.org/10.2478/acb-2021-0022","url":null,"abstract":"Abstract There are multiple possible applications of stem cells in medicine, from cell-based therapies for degenerative and dystrophic conditions, through novel approaches in cancer treatment, to in vitro organ printing. However, there are still several challenges that need to be overcame before stem cells therapies can be successfully introduced worldwide on a large scale. These include sourcing of stem cells, preventing their aberrant progression and ethical concerns regarding their use in animals and humans. Among the multiple stem cell types present in the human organism from the period of embryonic development to adulthood, this review focuses on the three types that gain the most attention in relation to modern research: embryonic stem cells, induced pluripotent stem cells and adult stem cells. There are a number of obstacles that need to be removed before these cells can be widely applied in clinical practice, including the choice of the perfect source of stem cells, full elucidation of the mechanisms of stem cell differentiation and plasticity, and minimization of adverse side effect potential. Nonetheless, the focus of the scientific community on the topic of stem cells remains unhindered, bringing hope that all of the possible concerns will be addressed in the future.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"9 1","pages":"160 - 164"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47347371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New gene markers involved in regulation of granulosa cells development and differentiation towards endodermal and epithelial tissues – a new insight into the stemness specificity of ovarian follicular cells 参与颗粒细胞向内胚层和上皮组织发育和分化调控的新基因标记——对卵巢滤泡细胞干性特异性的新认识

Medical Journal of Cell Biology Pub Date : 2021-12-01 DOI: 10.2478/acb-2021-0025

W. Kranc, M. Popis, C. Dompe, A. Golkar-Narenji, M. Ješeta, P. Mozdziak, E. Kistanova, A. Makarevich, M. Machatkova, D. Bukowska, R. Procházka, J. Jaśkowski, Kornel Ratajczak, J. Sobolewski, P. Antosik

{"title":"New gene markers involved in regulation of granulosa cells development and differentiation towards endodermal and epithelial tissues – a new insight into the stemness specificity of ovarian follicular cells","authors":"W. Kranc, M. Popis, C. Dompe, A. Golkar-Narenji, M. Ješeta, P. Mozdziak, E. Kistanova, A. Makarevich, M. Machatkova, D. Bukowska, R. Procházka, J. Jaśkowski, Kornel Ratajczak, J. Sobolewski, P. Antosik","doi":"10.2478/acb-2021-0025","DOIUrl":"https://doi.org/10.2478/acb-2021-0025","url":null,"abstract":"Abstract Maintaining of female fertility is strictly dependent on proper hormonal regulation. Granulosa cells (GCs) are components of ovarian follicles, and they are important in paracrine regulation of the ovary. Preovulatory follicle GCs are responsible for production of estrogens to the ovary microenvironment and lead to the LH surge. Proper functioning of GCs is necessary to ensure appropriate conditions for oocyte development, maturation, ovulation and its release to the oviduct. Long-term in vitro culture of GCs show significant stem-like characteristics. Understanding the molecular processes underlying GCs differentiation towards different cell lineages may reveal other possible stem cell markers. A transcriptomic analysis of short-term primary in vitro cultured GCs, which were isolated from porcine preovulatory follicles was the major focus of the study. The ontological groups herby considered are associated with endodermal and epithelial tissues. Results were and compare to freshly isolated GC cells. 6 the most reduced expression: HSD17B1, DAPL1, NEBL, MAL2, DAB1, ITM2A were chosen for analysis. These genes have been response for processes associated with GCs development and differentiation towards endodermal and epithelial tissues, which make them important for further consideration.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"9 1","pages":"177 - 187"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46332041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methods of detection and isolation of trophoblast cells from trans-cervical specimens – a historical overview 经宫颈标本中滋养细胞的检测和分离方法-历史概述

Medical Journal of Cell Biology Pub Date : 2021-12-01 DOI: 10.2478/acb-2021-0024

Rafał Sibiak, E. Wender-Ożegowska

引用次数: 0

Erratum to: The blood vessels development, morphogenesis and blood circulation are three ontologic groups highly up-regulated in porcine oocytes before in vitro maturation 勘误表：猪卵母细胞体外成熟前，血管发育、形态发生和血液循环是三个高度上调的个体组

Medical Journal of Cell Biology Pub Date : 2021-12-01 DOI: 10.2478/acb-2021-0029

B. Kempisty

引用次数: 0

Susceptibility of spike glycoprotein and RNA-dependent RNA polymerase of SARS-CoV-2 to mutation: in silico structural dynamics study SARS-CoV-2刺突糖蛋白和RNA依赖RNA聚合酶对突变的敏感性:硅结构动力学研究

Medical Journal of Cell Biology Pub Date : 2021-12-01 DOI: 10.2478/acb-2021-0020

T. Fatoki, Jude Akinyelu, O. Y. Adetuyi, T. O. Jeje, U. Nebo, Jesupemi Mercy Fatoki, Tolulope Mercy Kupolati

{"title":"Susceptibility of spike glycoprotein and RNA-dependent RNA polymerase of SARS-CoV-2 to mutation: in silico structural dynamics study","authors":"T. Fatoki, Jude Akinyelu, O. Y. Adetuyi, T. O. Jeje, U. Nebo, Jesupemi Mercy Fatoki, Tolulope Mercy Kupolati","doi":"10.2478/acb-2021-0020","DOIUrl":"https://doi.org/10.2478/acb-2021-0020","url":null,"abstract":"Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a microorganism that causes coronavirus disease 2019 (COVID-19). Mutations affect evolutionary conservation of microorganisms. The fast pace evolutionary changes are currently affecting pathogenicity of SARS-CoV-2. In this study, the structural fluctuations of the amino acid residues in the spike glycoprotein and RNA-dependent RNA polymerase (nsp12) of SARS-CoV-2 were investigated by in silico approach using structural flexibility dynamics to decipher susceptibility to mutation. The result of this study implicated key amino acid residues (with rmsf) which could be very susceptible to mutation, which include residues 50 (3.79 Å), 119 (4.56 Å), 120 (3.53 Å), 220 (3.84 Å), 265 (4.31 Å) of RNA-dependent RNA polymerase (nsp12), as well as residues 477 (4.21 Å), 478 (4.82 Å), 479 (5.40 Å), 481 (5.94 Å), 560 (4.63 Å), 704 (4.02 Å), 848 (4.58 Å), 1144 (4.56 Å) and 1147 (4.61 Å) of spike glycoprotein. The SARS-CoV-2 mutations destabilized the overall protein structure in multiples of amino acid residues which could interfere with active site leading to insensitivity or resistance to the inhibitors. Mutation T478K of Spike glycoprotein showed the highest deviation in the structure. Overall, spike glycoprotein has the highest number of mutations, and these variants could increase the risk to human health if not mitigated in the population.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"9 1","pages":"148 - 152"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47434674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endocrine disrupting chemicals and their detection in an IVF laboratory 内分泌干扰物及其在试管婴儿实验室的检测

Medical Journal of Cell Biology Pub Date : 2021-12-01 DOI: 10.2478/acb-2021-0023

M. Ješeta, K. Franzová, L. Mekiňová, E. Kistanova, Peter Harbulák, J. Žáková, E. Lousová, P. Ventruba, I. Crha

{"title":"Endocrine disrupting chemicals and their detection in an IVF laboratory","authors":"M. Ješeta, K. Franzová, L. Mekiňová, E. Kistanova, Peter Harbulák, J. Žáková, E. Lousová, P. Ventruba, I. Crha","doi":"10.2478/acb-2021-0023","DOIUrl":"https://doi.org/10.2478/acb-2021-0023","url":null,"abstract":"Abstract The endocrine disruptors are chemicals with the capacity to influence physiological processes in the organism, most often through hormonal control. They are present in the environment and in the products of daily use. They are often found in food, released from plastic bottles for water, present in cosmetics or fertilizers. Latest research suggests that they can be released from plastics used in the IVF laboratories and can be even present in the manipulation and cultivation media used for isolation and fertilization of gametes and subsequent cultivation of embryos. Permanent and long-term utilization of these substances has adverse effects in human reproductive health, mainly by the means of interfering with synthesis and action mechanisms of reproductive hormones. Moreover, some endocrine disruptors show a range of adverse effects directly on the gametes or embryos cultured in the in vitro conditions. The article provides an overview on bisphenols detected in plastics and media commonly used in the IVF laboratory and considers their possible impact on effectiveness of the IVF methods in a human laboratory.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"9 1","pages":"165 - 169"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42625530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum to: Fatty acids related genes expression undergo substantial changes in porcine oviductal epithelial cells during long-term primary culture 在猪输卵管上皮细胞的长期原代培养过程中，脂肪酸相关基因的表达发生了实质性的变化

Medical Journal of Cell Biology Pub Date : 2021-12-01 DOI: 10.2478/acb-2021-0027

B. Kempisty

引用次数: 0

Erratum to: Expression changes in fatty acid metabolic processrelated genes in porcine oocytes during in vitro maturation 勘误表：猪卵母细胞体外成熟过程中脂肪酸代谢过程相关基因的表达变化

Medical Journal of Cell Biology Pub Date : 2021-12-01 DOI: 10.2478/acb-2021-0026

B. Kempisty

引用次数: 0

Erratum to: Analysis of fructose and mannose – regulatory peptides signaling pathway in porcine epithelial oviductal cells (OECs) primary cultured long-term in vitro 勘误表：长期体外原代培养的猪输卵管上皮细胞（OECs）中果糖和甘露糖-调节肽信号通路的分析

Medical Journal of Cell Biology Pub Date : 2021-12-01 DOI: 10.2478/acb-2021-0028

B. Kempisty

引用次数: 0

The significance of HERC5, IFIH1, SAMD4, SEMA3A and MCTP1 genes expression in resistance to cytotoxic drugs in ovarian cancer cell lines HERC5、IFIH1、SAMD4、SEMA3A和MCTP1基因表达在卵巢癌症细胞系细胞毒性药物耐药性中的意义

Medical Journal of Cell Biology Pub Date : 2021-09-01 DOI: 10.2478/acb-2021-0019

M. Nowacka, B. Ginter-Matuszewska, M. Świerczewska, M. Nowicki, M. Zabel, Karolina Sterzyńska, R. Januchowski

{"title":"The significance of HERC5, IFIH1, SAMD4, SEMA3A and MCTP1 genes expression in resistance to cytotoxic drugs in ovarian cancer cell lines","authors":"M. Nowacka, B. Ginter-Matuszewska, M. Świerczewska, M. Nowicki, M. Zabel, Karolina Sterzyńska, R. Januchowski","doi":"10.2478/acb-2021-0019","DOIUrl":"https://doi.org/10.2478/acb-2021-0019","url":null,"abstract":"Abstract Resistance to chemotherapy is the main obstacle in contemporary ovarian cancer treatment. The aim of this study was the evaluation of expression of HERC5, IFIH1, SAMD4, MCTP1 and SEMA3A mRNA and assessment their role in resistance to cisplatin, paclitaxel, doxorubicin and topotecan in seven ovarian cancer cell lines. MTT assay was used in resistance assessment. Quantitative polymerase chain reaction was performed to measure the expression levels of the genes. We observed different levels of resistance among cell lines. The resistance was not related to the expression of drug transporters genes. The expression of HERC5 and IFIH1 genes was upregulated, and the expression of SEMA3A gene was downregulated. Expression of SAMD4 was upregulated in PEA1, PEA2, and PEO23 cell lines, and expression of MCTP1 was downregulated in A2780, PEA2, and PEO23 cell lines. Upregulation of HERC5, IFIH1, and SAMD4 and downregulation of SEMA3A and MCTP1 in TOP-resistant ovarian cancer cell lines may suggest some role of those genes in topotecan resistance development.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"9 1","pages":"138 - 147"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43869381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2