Medical Journal of Cell Biology最新文献_第4页

Ocular Manifestations of Pediatric Inflammatory Multisystem Syndrome 小儿炎性多系统综合征的眼部表现

Medical Journal of Cell Biology Pub Date : 2022-06-01 DOI: 10.2478/acb-2022-0010

J. Mamczur, Manuela Pajdowska, Aleksandra Krasińska, A. Brązert

引用次数: 0

Difficulties in Treatment of Iridocorneal Endothelial Syndrome - Case Report 虹膜内皮综合征的治疗难点——病例报告

Medical Journal of Cell Biology Pub Date : 2022-06-01 DOI: 10.2478/acb-2022-0009

Aleksandra Krasińska, J. Mamczur, Manuela Pajdowska, A. Brązert

{"title":"Difficulties in Treatment of Iridocorneal Endothelial Syndrome - Case Report","authors":"Aleksandra Krasińska, J. Mamczur, Manuela Pajdowska, A. Brązert","doi":"10.2478/acb-2022-0009","DOIUrl":"https://doi.org/10.2478/acb-2022-0009","url":null,"abstract":"Abstract The iridocorneal endothelial syndrome manifests in three clinical types: Chandler syndrome, progressive iris atrophy, and Cogan-Reese syndrome. It is caused by the presence of abnormal corneal endothelium on the iris stroma and anterior chamber angle leading to usually unilateral, progressive iris atrophy, glaucoma, and/or corneal edema. The etiology is yet unclear. It affects mostly young adults, mostly females. Management of iridocorneal endothelial syndrome is complex: conservative and surgical, depending on the stage of the disease and intensity of present symptoms. A 30-year-old female with a medical history of the iridocorneal endothelial syndrome was reported to the Ophthalmology Department for consultation. Slit-lamp examination revealed iris atrophy and superior-nasal corectopia in the left eye. On gonioscopy, the angle was wide open in the right eye, but there were iridocorneal adhesions and incomplete angle-closure in the left eye. The patient was provided with maximum local therapy consisting of three anti-glaucoma medications. On later check-ups, the patient presented corneal edema and increased intraocular pressure. She was qualified to ExPress mini shunt trabeculectomy with mitomycin C. Two years later, a patient came to the clinic because of increased values of intraocular pressure (up to 59 mmHg), slit-lamp examination showed that the ExPress implant was congested with fragments of the corneal endothelial cells. Thanks to YAG iridotomy the implant was recanalized.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"10 1","pages":"56 - 60"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49283439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Influence of L-Methionine, DL-Methionine, and a Methionine Hydroxy Analog on Proliferation and Differentiation Potential of Avian Myoblasts l -蛋氨酸、dl -蛋氨酸和一种蛋氨酸羟基类似物对禽成肌细胞增殖和分化潜能的影响

Medical Journal of Cell Biology Pub Date : 2022-06-01 DOI: 10.2478/acb-2022-0012

Ronald J. Savino, M. Vázquez-Añón, K. Stefańska, J. Petitte, P. Mozdziak

{"title":"The Influence of L-Methionine, DL-Methionine, and a Methionine Hydroxy Analog on Proliferation and Differentiation Potential of Avian Myoblasts","authors":"Ronald J. Savino, M. Vázquez-Añón, K. Stefańska, J. Petitte, P. Mozdziak","doi":"10.2478/acb-2022-0012","DOIUrl":"https://doi.org/10.2478/acb-2022-0012","url":null,"abstract":"Abstract Methionine is an essential amino critical to many cell functions including the synthesis of proteins. Supplementation of methionine in vivo is typically through L-methionine, DL-methionine, or a methionine hydroxy analog (MHA). The goal of this study was to compare the function of L-methionine, DL-methionine, and an MHA as a source of methionine to myoblasts in vitro. Avian myoblasts isolated from turkey embryos were plated in media containing varying concentrations of DL-methionine (DLM; 1.125 mg/mL or 0.56 mg/mL) or methionine hydroxy analog (MHA; 1.28 mg/mL or 0.64 mg/mL) as well as a methionine deficient negative control group and an L-methionine supplemented positive control group. The results of the proliferation assay exhibited cell division in the absence of methionine which was not significantly different than the positive control group. Results from the myoblast fusion assay revealed significantly greater myotube diameter between methionine supplemented groups compared to the methionine deficient negative control. The findings of this study show an ability for avian myoblasts to proliferate in the absence of methionine, the significance of which is discussed. Additionally, findings from the fusion assay suggest that DL-methionine and MHA are potential cost-effective substitutes for methionine supplementation during terminal differentiation of avian myoblasts.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"10 1","pages":"69 - 82"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41758833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ELABELA as a Marker of Gestational Complications – A Review ELABELA作为妊娠并发症的标志-综述

Medical Journal of Cell Biology Pub Date : 2022-06-01 DOI: 10.2478/acb-2022-0007

Rafał Sibiak

{"title":"ELABELA as a Marker of Gestational Complications – A Review","authors":"Rafał Sibiak","doi":"10.2478/acb-2022-0007","DOIUrl":"https://doi.org/10.2478/acb-2022-0007","url":null,"abstract":"Abstract Adipose tissue secretes dozens of biologically active molecules known as adipokines or adipocytokines. Apelin receptor early endogenous ligand (ELABELA, also known as ELA or APELA) is a circulating signaling protein expressed in placental tissue that binds to apelin receptors. The first animal experimental findings suggested that the ELABELA deficiency might be responsible for the pathogenesis of preeclampsia--like symptoms, i.e., hypertension and proteinuria in mice. Exogenous ELABELA supplementation reverted preeclampsia symptoms and normalized fetal birth weight in mice. Several in vitro studies confirmed that ELABELA supplementation could improve trophoblast cell functions such as invasiveness and proliferation capacity. Thus, the ELABELA axis could serve as the target of innovative therapies for gestational complications. Nonetheless, most human studies do not support the thesis that disturbances in ELABELA secretion in early pregnancy are associated with an increased risk of preeclampsia. Therefore, it is unlikely that ELABELA could serve as a novel early marker of preeclampsia in humans. Alterations in the ELABELA secretion have also been discovered among patients with other gestational complications such as GDM and fetal growth restriction.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"10 1","pages":"43 - 48"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45908640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes of the zona pellucida patterns during oocyte maturation, fertilization and embryo development in mammals: mini-review 哺乳动物卵母细胞成熟、受精和胚胎发育过程中透明带模式的变化：综述

Medical Journal of Cell Biology Pub Date : 2022-03-01 DOI: 10.2478/acb-2022-0004

Ivona Travnickova, P. Hulínská, Z. Sládek, M. Skowroński, M. Machatkova

引用次数: 0

Approaches for in vitro culture of granulosa cells and ovarian follicles 颗粒细胞和卵巢卵泡体外培养的方法

Medical Journal of Cell Biology Pub Date : 2022-03-01 DOI: 10.2478/acb-2022-0006

Wiktoria Zgórecka, M. Ješeta, R. Procházka, C. Amorim, Kornelia Krajnik, P. Mozdziak, Wojciech Pieńskowski, M. Skowroński, W. Kranc

引用次数: 1

Current application of exosomes in medicine 外泌体在医学中的应用现状

Medical Journal of Cell Biology Pub Date : 2022-03-01 DOI: 10.2478/acb-2022-0003

M. Jankowski, A. Volponi, Rafael Shinoske Siroma, Nelson Pinto, M. A. Melo, Kornelia Krajnik, J. Shibli, P. Mozdziak, M. Skowroński, M. Dyszkiewicz-Konwińska

{"title":"Current application of exosomes in medicine","authors":"M. Jankowski, A. Volponi, Rafael Shinoske Siroma, Nelson Pinto, M. A. Melo, Kornelia Krajnik, J. Shibli, P. Mozdziak, M. Skowroński, M. Dyszkiewicz-Konwińska","doi":"10.2478/acb-2022-0003","DOIUrl":"https://doi.org/10.2478/acb-2022-0003","url":null,"abstract":"Abstract Exosomes are a distinct type of extracellular vesicles that play a major role in intracellular transport and communication. Depending on the cell of origin, exosomes can contain diverse constituents of a cell, including DNA, RNA, lipids, metabolites, cytosolic and cell-surface proteins, playing important roles in a wide range of physiological and pathological processes. Due to these facts, they are subject of extensive research aiming at translating the knowledge into clinical approaches that are at the interface between nanomedicine and biopharmaceuticals. Their potential clinical use mostly revolves around the fields of diagnostics and drug delivery, especially important in treatment of cancer. The conventional and emerging methods of exosome isolation are either based on their physical properties (such as density and/or size) or their functions. However, the isolation approaches are still characterised by significant downsides, lacking standardisation, and ensuring purity. The review gives a critical overview on exosomes characteristics, isolation approaches and the potential that exosomes hold in developing new clinical approaches of modern medicine, highlighting the need for further research to fully grasp their potential and translate the knowledge into future therapeutic solutions.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"10 1","pages":"18 - 22"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42473769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Follicular renewal and stemness potency of follicular cells depended of telomerase activity and TERT expression – short review 端粒酶活性和TERT表达对卵泡细胞再生和干性的影响

Medical Journal of Cell Biology Pub Date : 2022-03-01 DOI: 10.2478/acb-2022-0005

Wiktoria Zgórecka, A. G. Narenji, Kornelia Krajnik, P. Antosik, D. Bukowska, M. Grzesiak, R. Procházka, P. Mozdziak, M. Skowroński, W. Kranc

{"title":"Follicular renewal and stemness potency of follicular cells depended of telomerase activity and TERT expression – short review","authors":"Wiktoria Zgórecka, A. G. Narenji, Kornelia Krajnik, P. Antosik, D. Bukowska, M. Grzesiak, R. Procházka, P. Mozdziak, M. Skowroński, W. Kranc","doi":"10.2478/acb-2022-0005","DOIUrl":"https://doi.org/10.2478/acb-2022-0005","url":null,"abstract":"Abstract Several hypotheses have been proposed, relating to the potential genesis of follicular cells in the ovarian niche. Reports using mice as an experimental model have suggested that the ovaries may contain stem cells that are likely involved in the formation of new follicles in adult reproductive life. Over recent years, various types of ovarian cells have been identified and described to confirm or disprove the existence of ovarian adult stem cells. Most research is focused on granulosa cells (GCs), which are essential for follicular development and maturation of female germ cells (oocytes). GCs exhibit the features of stem cells, such as expression of stem cell markers: OCT-4, Sox-2, Nanog as well as certain markers of mesenchymal stem cells, including CD29, CD44, CD90, CD105, CD117, and CD166. Another discovery in favor of the potential stemness of GCs is their ability to transdifferentiate towards other cell lines and high telomerase (TERT) activity in dividing compartments of the follicle during its maturation.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"10 1","pages":"29 - 33"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44521580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silica nanoparticles in targeted human cancer therapy 纳米二氧化硅在人类癌症靶向治疗中的应用

Medical Journal of Cell Biology Pub Date : 2022-03-01 DOI: 10.2478/acb-2022-0002

H. Yehia, Said Mahmoud Said, Noheer Galal Elden Rashad Ragb

引用次数: 2

Viability of bovine in vitro matured oocytes following ultra-rapid vitrification 牛体外成熟卵母细胞超快速玻璃化后的存活率

Medical Journal of Cell Biology Pub Date : 2022-03-01 DOI: 10.2478/acb-2022-0001

L. Dujíčková, L. Olexiková, E. Kubovicova, J. Bezdíček, M. Ješeta, A. Makarevich

{"title":"Viability of bovine in vitro matured oocytes following ultra-rapid vitrification","authors":"L. Dujíčková, L. Olexiková, E. Kubovicova, J. Bezdíček, M. Ješeta, A. Makarevich","doi":"10.2478/acb-2022-0001","DOIUrl":"https://doi.org/10.2478/acb-2022-0001","url":null,"abstract":"Abstract The aim of the study was to examine viability of cattle oocytes after cryopreservation. Oocytes after in vitro maturation (IVM) were vitrified in minimum volume on the nickel electron microscopy grids by ultra-rapid cooling technique. After warming and subsequent in vitro fertilization the presumptive zygotes were cultured to reach the stage of the blastocyst (Bl). Several devitrified oocytes were processed for electron microscopy assay. Although, embryo cleavage and Bl percentages in the vitrified group were slightly lower than in the control group (P < 0.05), the Bl total cell number (TCN), apoptosis and dead cell percentages did not differ between both groups. However, significant difference was found between day 7 (D7) and day 8 (D8) Bl in the TCN in control (108.0 vs. 90.5) and vitrified group (103.75 vs 98.14). Electron microscopy of frozen oocytes revealed slight reversible injuries in mitochondria and the smooth endoplasmic reticulum (SER), nevertheless, the development of devitrified oocytes to the Bl stage was comparable to those in fresh oocytes. In conclusion, higher proportion of slower developing Bl (D8) compared to D7 Bl may be related to the mentioned minor damages of some organelles in vitrified oocytes.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"10 1","pages":"1 - 6"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49476556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0