Medical Journal of Cell Biology最新文献_第3页

Expression of selected cytokeratins in human placenta - a preliminary observational study 人胎盘细胞角蛋白表达的初步观察研究

Medical Journal of Cell Biology Pub Date : 2022-12-01 DOI: 10.2478/acb-2022-0023

Z. Domagała, Andrzej Mrozek, A. Piotrowska, Natalia Olesińska, J. Domański, C. Kobierzycki, Michelle Thelen, Jakub Śliwa

{"title":"Expression of selected cytokeratins in human placenta - a preliminary observational study","authors":"Z. Domagała, Andrzej Mrozek, A. Piotrowska, Natalia Olesińska, J. Domański, C. Kobierzycki, Michelle Thelen, Jakub Śliwa","doi":"10.2478/acb-2022-0023","DOIUrl":"https://doi.org/10.2478/acb-2022-0023","url":null,"abstract":"Abstract Every human body is made up of billions of cells, and every cell consists of thousands of microscopic structures. Thanks to the presence of the cytoskeleton, which is built by microfilaments, microtubules, and intermediate filaments, cells are able to fulfill their main function. Dozens of genes encode a large family of cytoskeletal proteins, which form 10-nanometer-long microfilaments, called cytokeratins. The study was carried out on seven mature human placentas without significant pathology - all eligible mothers were healthy. The collection of basic anthropometric data preceded the dissection of the placentas. Paraffin blocks were made in the usual manner, and hematoxylin and eosin-stained slides were made afterward. Immunohistochemical reactions were performed and the expression of the studied markers was evaluated independently by two observers. Evaluation of microscopic material revealed the absence of expression of antibodies for cytokeratin 5/6 in placental tissues. Moreover, strong expression of cytokeratin 7 was demonstrated in the villi trophoblast in all types of villi. Immunohistochemical reactions were observed in the mesenchyme within the blood vessel wall, as well as in the extravascular tissue. The human placenta is an organ that only exists during the intrauterine period of human development and undergoes rapid changes and dynamic growth during pregnancy. These types of processes define selected placental cells as ‘pseudo-tumorigenic tissue’ because of the numerous similarities trophoblast cells have to tumor cells. The use of tissue microarray (TMA) in combination with immunohistochemistry (IHC) may be a valuable approach to validate the predictive and diagnostic utility of various biomarkers in non-cancerous tissues like placental tissue.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"10 1","pages":"155 - 162"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46042963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trigger points in medical practice - current therapeutic directions 医学实践中的触发点-当前治疗方向

Medical Journal of Cell Biology Pub Date : 2022-12-01 DOI: 10.2478/acb-2022-0020

M. Wanat, Bernadetta Nowak, Adam Swiatko, K. Mirkowski, J. Domański, P. Dąbrowski, Z. Domagała

{"title":"Trigger points in medical practice - current therapeutic directions","authors":"M. Wanat, Bernadetta Nowak, Adam Swiatko, K. Mirkowski, J. Domański, P. Dąbrowski, Z. Domagała","doi":"10.2478/acb-2022-0020","DOIUrl":"https://doi.org/10.2478/acb-2022-0020","url":null,"abstract":"Abstract Recent scientific evidence suggests that one of the causes of myofascial pain syndromes responsible for the occurrence of chronic headaches, among other things, may be increased or impaired with trigger point therapy. This paper presents the results of a non-systematic literature review on trigger point therapy. The analysis shows a clear increase in scientific interest in invasive trigger point pain treatment techniques. Invasive therapy consists primarily of the use of injections and dry needling. Injections of anesthetics or botulinum toxin are methods that, in addition to their possible therapeutic effect, may carry side effects. In contrast, dry needling does not have a fully scientifically-proven effect. Recent scientific data also indicates that the aforementioned method may have a limited clinical effect. The performed analysis did not demonstrate a significant advantage in invasive techniques over typical non-invasive techniques. It is therefore necessary to design randomized multi-center clinical trials to definitively verify the efficacy of these techniques in the treatment of tension-type trigger point-dependent myofascial pain.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"10 1","pages":"129 - 137"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48962099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human ovarian follicular granulosa cells isolated during ART procedure reflect substantial changes in activation of hormonal signaling pathways, during long-term in vitro conditions 在长期体外条件下，在ART过程中分离的人卵巢卵泡颗粒细胞反映了激素信号通路激活的实质性变化

Medical Journal of Cell Biology Pub Date : 2022-12-01 DOI: 10.2478/acb-2022-0024

Wiktoria Zgórecka, Małgorzata Blatkiewicz, M. Jankowski, W. Kranc, A. Bryja, M. Brązert, B. Chermuła, W. Pieńkowski, L. Pawelczyk, P. Mozdziak

{"title":"Human ovarian follicular granulosa cells isolated during ART procedure reflect substantial changes in activation of hormonal signaling pathways, during long-term in vitro conditions","authors":"Wiktoria Zgórecka, Małgorzata Blatkiewicz, M. Jankowski, W. Kranc, A. Bryja, M. Brązert, B. Chermuła, W. Pieńkowski, L. Pawelczyk, P. Mozdziak","doi":"10.2478/acb-2022-0024","DOIUrl":"https://doi.org/10.2478/acb-2022-0024","url":null,"abstract":"Abstract The ovary is commonly known as an endocrine gland responsible for sex steroid production. One of the outstanding cells in ovarian microenvironment - granulosa cells (GCs) are responsible for converting the androgens to estrogens during follicular growth and secreting progesterone after ovulation. These secretory processes within the ovary are directly involved in hormonal signaling pathways, and they depend on different stages of cholesterol and lipid biosynthesis during the ovarian cycle. The understating of the regulation and further investigation into the processes taking part in ovary will expose new clinical advantages in detection and treatment of female reproductive system diseases associated with sex hormone abnormalities. The expression of genes belonging to ontology groups associated with steroid biosynthesis and metabolism, such as “cholesterol biosynthetic process” (GO:0006695, “regulation of lipid biosynthetic process” (GO:0046890), “regulation of lipid metabolic process” (GO:0019216), “response to insulin” (GO:0032868) and “response to lipopolysaccharide” (GO:0032496) were analyzed by using the microarray approach. The patterns of gene expression in human GCs at days 1-day, 7-day, 15-day, and 30-day of primary in vitro culture have been analyzed. Based on the microarray results, a group of upregulated genes have been selected: CCL20, CXCL5, STAR, MSMO1, and AADAC. The genes STAT5B, OPA3, PPARG, PROX1, and SEC14L2 were decreased across all the experimental groups during the 30-day cell cultivation period. These results suggest that, the GCs in cell culture under in vitro express steroidogenic markers and it is important to understand associations with lipid and liposaccharide synthesis relative to reproductive medicine.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"10 1","pages":"163 - 175"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42495677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cadaver preserving methods – is it possible to do anything better? 保存尸体的方法——有可能做得更好吗?

Medical Journal of Cell Biology Pub Date : 2022-12-01 DOI: 10.2478/acb-2022-0019

Andrzej Mrozek, Mikołaj Sędzik, Jakub Rubin, Szymon Buras, Victoria Tarkowski, Z. Domagała

{"title":"Cadaver preserving methods – is it possible to do anything better?","authors":"Andrzej Mrozek, Mikołaj Sędzik, Jakub Rubin, Szymon Buras, Victoria Tarkowski, Z. Domagała","doi":"10.2478/acb-2022-0019","DOIUrl":"https://doi.org/10.2478/acb-2022-0019","url":null,"abstract":"Abstract It is impossible to imagine learning anatomy without properly fixating cadavers. Thanks to accurate preservation techniques, students can differentiate anatomical structures by their sight and touch. The formalin method was first described almost 150 years ago, and as such it may seem primitive, however it is effective and easy to use, which is why it is still in use today. Despite formaldehyde’s bactericidal, fungicidal, and insecticidal properties contact with vapors of embalming solutions such as formalin may have a negative health effect. Skin drying, eczema, allergic contact dermatitis and lowered red blood cells (RBCs) and platelets are only a few symptoms that may occur as a result of spending long periods of time in places where cadavers are stored. Due to formalin’s features, other techniques were invented. Thiel’s method is also well known; after liquid application, tissues remain their natural color as the method is non-irritating and almost odorless. With the rise of technology, more modern methods of cadaver preservation were developed as alternative to formalin, such as the use of N-vinyl-2-pyrrolidone (NVP), which is commonly implemented in Japan and Brazil, and the Modified Larssen solution (MLS), which gives the possibility to reduce formalin concentration without losing the effectiveness of tissue preservation. The aim of this work is to develop an integrated approach to cadaver preservation through the analysis of frequently used preservation techniques. This is a considerable issue because first-year students of medicine and related faculties encounter the preserved material while learning anatomy. In comparison to more modern methods, formalin seems to be the worst choice due to worse joint flexibility, tissue colour and structure and, most importantly, its cancerogenic action.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"10 1","pages":"124 - 128"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42630460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Expression profile of genes regulating cellular response to cytokine stimulus in Sjögren’s syndrome 干燥综合征细胞对细胞因子刺激反应调控基因的表达谱

Medical Journal of Cell Biology Pub Date : 2022-12-01 DOI: 10.2478/acb-2022-0022

K. Błochowiak, M. Nawrocki, P. Celichowski, W. Samborski, K. Iwanik, D. Sikorska, Marek Rabski, K. Jopek

{"title":"Expression profile of genes regulating cellular response to cytokine stimulus in Sjögren’s syndrome","authors":"K. Błochowiak, M. Nawrocki, P. Celichowski, W. Samborski, K. Iwanik, D. Sikorska, Marek Rabski, K. Jopek","doi":"10.2478/acb-2022-0022","DOIUrl":"https://doi.org/10.2478/acb-2022-0022","url":null,"abstract":"Abstract The existence of abnormal cytokine profile in Sjögren’s syndrome (SS) may contribute to find potential SS biomarkers and accompanying comorbidities. We aimed to investigate and compare gene expressions in the labial salivary glands of SS and of healthy subjects (HS) by means of microarray analysis. The study group comprised 8 SS patients and 8 HS in microarray analysis. The relative gene expression changes were validated with real time quantitative polymerase chain reaction in 25 SS and 20 HS. Among the differently expressed genes belonging to the “cellular response to cytokine stimulus”, “cytokine-mediated signaling pathway” and “innate immune response” ontology groups with a fold change >1.5 and with a p value < 0.05, the adiponectin, C1Q and collagen domain containing (ADIPOQ), S100 calcium binding protein A9 (S100A9), leptin receptor (LEPR), and fatty acid binding protein 4 (FABP4) gene expressions in SS group were higher than in HS. Increased expression of the assessed genes are determined by the presence of SS and the accompanying inflammation and may be a valuable diagnostic biomarker of SS. Their selected systemic effects may suggest their role as potential prognostic biomarkers of the risk of accelerated atherosclerosis and cardiovascular complications in SS.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"10 1","pages":"144 - 154"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46060551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Features of the flexor retinaculum and its individual variation in carpal tunnel syndrome predisposition - a systematic review 屈肌支持带的特征及其在腕管综合征易感性中的个体差异-一项系统综述

Medical Journal of Cell Biology Pub Date : 2022-09-01 DOI: 10.2478/acb-2022-0015

R. Krupa, Małgorzata Suchanecka, Andrzej Mrozek, K. Siwek

引用次数: 1

In vitro evaluation of biocompatibility of two intracanal medicaments on stem cells from apical papilla – a comparative study 两种肛门内药物对根尖乳头干细胞生物相容性的体外评价——一项比较研究

Medical Journal of Cell Biology Pub Date : 2022-09-01 DOI: 10.2478/acb-2022-0013

M. Dencheva, Evgeni Stanev, T. Georgieva

引用次数: 0

Anatomy of the coronary arteries in Eurasian wild boar (Sus scrofa) 欧亚野猪冠状动脉的解剖

Medical Journal of Cell Biology Pub Date : 2022-09-01 DOI: 10.2478/acb-2022-0014

P. Kaźmierczak, A. Butkiewicz, H. Frąckowiak, M. Zdun

{"title":"Anatomy of the coronary arteries in Eurasian wild boar (Sus scrofa)","authors":"P. Kaźmierczak, A. Butkiewicz, H. Frąckowiak, M. Zdun","doi":"10.2478/acb-2022-0014","DOIUrl":"https://doi.org/10.2478/acb-2022-0014","url":null,"abstract":"Abstract Coronary circulation determines the proper functioning of the heart muscle and blood circulation in the body. Cardiovascular diseases in humans are creating an ever-increasing demand for more research into their pathogenesis, diagnosis and treatment. Animal models of organs are an indispensable part of research for the development of human medicine. Due to the high genetic similarity, the most commonly used donor is the domestic pig. The Eurasian wild boar is the ancestor of the domestic pig and an invasive species not covered by European protection programmes. Given its analogous structure and genotype to the pig, it could potentially also become an organ donor. The aim of this study was to analyse the normal coronary anatomy of the Eurasian wild boar and compare it to humans, domestic pigs and other animal species. The study was conducted on 60 wild boar hearts. Twenty-eight slides were prepared using acrylic, while liquid latex was injected into 32 hearts. The blood vessels were then manually prepared using surgical instruments for examination. Both the right coronary artery and the left coronary artery of the boar diverge from the ascending aorta from opposite aortic sinuses. The left coronary artery begins its course in the left aortic sinus, starting between the left appendage and the pulmonary trunk. It then heads inferiorly. The right coronary artery supplies blood to the right atrium and right ventricle. The study points to the wild boar coronary system’s individual morphological features and notes the common arterial patterns in mammals.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"10 1","pages":"89 - 96"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44493843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heavy Eye Syndrome – Case Report 重眼综合征1例报告

Medical Journal of Cell Biology Pub Date : 2022-06-01 DOI: 10.2478/acb-2022-0011

J. Mamczur, Aleksandra Krasińska, M. Pawlak

引用次数: 0

Potential Role of LYN, CCL2, ITGB3 and IL6 Genes in the Immune Response of Porcine Buccal Mucosa Cells LYN、CCL2、ITGB3和IL6基因在猪颊粘膜细胞免疫反应中的潜在作用

Medical Journal of Cell Biology Pub Date : 2022-06-01 DOI: 10.2478/acb-2022-0008

B. Borowiec, Rut Bryl, K. Stefańska, M. Dyszkiewicz-Konwińska

{"title":"Potential Role of LYN, CCL2, ITGB3 and IL6 Genes in the Immune Response of Porcine Buccal Mucosa Cells","authors":"B. Borowiec, Rut Bryl, K. Stefańska, M. Dyszkiewicz-Konwińska","doi":"10.2478/acb-2022-0008","DOIUrl":"https://doi.org/10.2478/acb-2022-0008","url":null,"abstract":"Abstract The genes considered in this study, namely, LYN, CCL2, ITGB3 and IL6 can be related to immune response in porcine buccal mucosa cells primary cultured in vitro. These genes are also responsible for, inter alia, cell migration, differentiation, proliferation and apoptosis, intracellular signal transduction, extracellular matrix binding and wound healing. A total of 20 pubertal crossbred Landrace gilts bred on commercial farms were used to obtain buccal mucosa cell cultures, which were harvested on the 7th, 15th and 30th day after initiation of the culture. Expression levels of LYN, CCL2, ITGB3 and IL6 were evaluated employing Real-Time Quantitative Polymerase Chain Reaction. All studied genes showed expression. The expression of CCL2 on day 15 was the highest of all factors measured. The greatest difference between the measurements occurred in gene IL6, between 7th and 15th day, while the least difference between the measurements occurred in gene LYN, between 7th and 15th day. Moreover, on the 7th day, LYN presented the lowest expression among all studied genes. Although in vitro conditions are much more controlled than in vivo conditions, all the factors that may positively or negatively affect cultured cells still cannot be fully predicted. Nevertheless, LYN, CCL2, ITGB3 and IL6 are a valuable starting point for studying further immunological processes in oral mucosal epithelial cells. Given their high regenerative potential, research into them is a highly valuable source of information for future wound healing therapies, where immunological processes should be carefully considered.","PeriodicalId":18329,"journal":{"name":"Medical Journal of Cell Biology","volume":"10 1","pages":"49 - 55"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49017232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0