Meat and Muscle Biology最新文献

筛选
英文 中文
Effects of Pork Quality Types, Packaging, and Degree of Doneness on Trained Sensory and Instrumental Tenderness, Color, and Consumer Perception 猪肉质量类型、包装和熟度对训练感官和工具嫩度、颜色和消费者感知的影响
Meat and Muscle Biology Pub Date : 2021-06-08 DOI: 10.22175/mmb.11598
A. Cassens, R. Ramanathan, D. Vanoverbeke, G. Mafi
{"title":"Effects of Pork Quality Types, Packaging, and Degree of Doneness on Trained Sensory and Instrumental Tenderness, Color, and Consumer Perception","authors":"A. Cassens, R. Ramanathan, D. Vanoverbeke, G. Mafi","doi":"10.22175/mmb.11598","DOIUrl":"https://doi.org/10.22175/mmb.11598","url":null,"abstract":"The objective of this study was to evaluate the palatability and retail display differences of 3 types of pork loins. Enhanced (n = 10), nonenhanced (n = 10), and high-quality (n = 10) pork loins were selected from a commercial food distribution company. Loins were cut into 2.54-cm-thick chops and randomly assigned to retail display, sensory, or Warner-Bratzler shear force analyses. For retail display, chops were randomly assigned to 1 of 3 packaging treatments: polyvinyl chloride overwrap, carbon monoxide-modified atmosphere packaging, and high-oxygen modified atmospheric packaging. Instrumental and visual color measurements were taken during 5 d of retail display. For trained sensory panel and Warner-Bratzler shear force analyses, chops were evaluated at 3 different degrees of doneness (63°C, 68°C, and 74°C). Consumer sensory panelists (N = 50) evaluated the overall liking, flavor, juiciness, and tenderness of chops cooked to 68°C. A treatment × packaging interaction (P < 0.05) occurred for L* and a* values. High-quality chops packaged in polyvinyl chloride overwrap were lighter in color (P < 0.05) compared with other treatment and packaging types. However, high- quality chops had lower (P < 0.05) a* values than other treatment and packaging types during retail display. Trained panelists reported no difference (P > 0.05) in tenderness for enhanced chops when cooked at 3 different degrees of doneness. Consumer panelists preferred the enhanced chops for overall liking, tenderness, and juiciness (P < 0.05) compared with high-quality and nonenhanced chops. The results suggest that enhanced, nonenhanced, and high-quality pork loins avail- able in the market vary in color and marbling. Both pork loin type and packaging can influence display color and palatability.","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81205453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Abstracts from the 2020 International Congress of Meat Science and Technology and the AMSA Reciprocal Meat Conference 摘要来自2020年国际肉类科学与技术大会和AMSA互惠肉类会议
Meat and Muscle Biology Pub Date : 2021-06-07 DOI: 10.22175/mmb.11683
Deidrea Mabry
{"title":"Abstracts from the 2020 International Congress of Meat Science and Technology and the AMSA Reciprocal Meat Conference","authors":"Deidrea Mabry","doi":"10.22175/mmb.11683","DOIUrl":"https://doi.org/10.22175/mmb.11683","url":null,"abstract":"The International Congress of Meat Science and Technology (ICoMST)/73rd Reciprocal Meats Conference (RMC) was hosted August 2–7, 2020 by the American Meat Science Association. This historic conference was held virtually because of the unprecidented global Covid-19 pandemic. The abstracts for the over 200 scientific papers that were presented at the meeting are contained in this document.","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75061722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Palatability Traits of Sirloin Cap Steaks From Four USDA Quality Grades 美国农业部四个质量等级的西冷牛排的适口性特征
Meat and Muscle Biology Pub Date : 2021-05-25 DOI: 10.22175/MMB.12424
E. Beyer, K. Harr, B. Olson, E. Rice, Cassandra K. Jones, Michael D. Chao, J. Vipham, M. Zumbaugh, T. O’Quinn
{"title":"Palatability Traits of Sirloin Cap Steaks From Four USDA Quality Grades","authors":"E. Beyer, K. Harr, B. Olson, E. Rice, Cassandra K. Jones, Michael D. Chao, J. Vipham, M. Zumbaugh, T. O’Quinn","doi":"10.22175/MMB.12424","DOIUrl":"https://doi.org/10.22175/MMB.12424","url":null,"abstract":"Sirloin cap steaks from 4 different USDA quality grades were evaluated to determine the effect of marbling on eating experience. Top sirloin cap steaks (N = 60) of 4 different quality grades (Prime, Top Choice, Low Choice, and Select) were evaluated in the current study. Top sirloin butts were collected, and the biceps femoris was removed, trimmed, and sliced into twelve 2.54-cm-thick steaks. The steaks from each subprimal were aged for 28 d and designated for either trained sensory panels, consumer sensory panels, Warner-Bratzler shear force, or moisture and fat determination. The Prime steaks were rated the highest (P < 0.05) for overall liking and were higher (P < 0.05) for flavor liking than Low Choice and Select steaks within the consumer panels. However, all quality treatments were similar (P > 0.05) in juiciness and tenderness for the consumer panels. Additionally, a similar (P > 0.05) percentage of samples from each treatment were rated acceptable for tenderness, juiciness, flavor liking, and overall liking, with each trait having greater than 70% of samples rated acceptable. Also, Prime steaks were rated the highest (P < 0.05) for sustained juiciness and overall tenderness within the trained sensory panels. Similarly, Prime steaks were rated higher (P <0.05) for initial juiciness than the Low Choice and Select treatments but were similar (P > 0.05) to Top Choice. These results indicate that USDA quality grades impact the eating quality of sirloin cap steaks, especially within the Prime grade, and could provide an opportunity for refined marketing and added economic value for the cut.","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87022357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Development of beef volatile flavor compounds in response to varied oven temperature and degree of doneness 牛肉挥发性风味化合物对不同烤箱温度和熟度的响应
Meat and Muscle Biology Pub Date : 2021-05-25 DOI: 10.22175/MMB.12365
S. Mallick, D. Woerner, Talita de Mancilha Franco, Mark F. Miller, J. Legako
{"title":"Development of beef volatile flavor compounds in response to varied oven temperature and degree of doneness","authors":"S. Mallick, D. Woerner, Talita de Mancilha Franco, Mark F. Miller, J. Legako","doi":"10.22175/MMB.12365","DOIUrl":"https://doi.org/10.22175/MMB.12365","url":null,"abstract":"Beef volatile flavor compound (VFC)development at the center, mid, and surface layers of cooked steaks wasevaluated through eighteen cookery treatment combinations consisting of oven cookingtemperature (OT; 177°C, 246°C, and 343°C) and final internal steak temperature(IT; 57°C, 63°C, 68°C, 74°C, 79°C, 85°C). In total, seventy-two VFC were measuredrepresenting the Maillard reaction and lipid degradation. Five VFC wereimpacted by a three-way interaction of OT × IT × layer (P ≤ 0.030). TwoVFC were impacted by a two-way interaction OT × IT (P ≤ 0.010). SixteenVFC were impacted by a two-way interaction OT × layer (P ≤ 0.050). SixteenVFC were impacted by a two-way interaction IT × layer (P ≤ 0.050).Twenty VFC were impacted by main effect of layer (P ≤ 0.010). Eight VFCwere impacted by main effect of IT (P ≤ 0.050). Maillard compounds were formedprimarily at steak surfaces with a general increase in content with greater finalIT and OT to a lesser extent. Lipid derived compounds were diverse. Methyl estersand aldehydes were consistently in lower content at steak surfaces and primarilyfound within the inner portions of steaks. Conversely, certain alcohols and ketoneswere more prominent at steak surfaces. Development of compounds among layers wasalso consistently influenced by IT and OT. It may be concluded that flavor contributingcompounds vary among cooked beef steaks at different depths and cookery temperatures,such as OT and final IT, may be utilized to mediate the final volatile compoundcomposition. ","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86762023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Partial Purification of Peroxiredoxin-2 From Porcine Skeletal Muscle 猪骨骼肌中过氧化物还毒素2的部分纯化
Meat and Muscle Biology Pub Date : 2021-05-06 DOI: 10.22175/MMB.12408
L. Johnson, E. Steadham, E. Huff-Lonergan, S. Lonergan
{"title":"Partial Purification of Peroxiredoxin-2 From Porcine Skeletal Muscle","authors":"L. Johnson, E. Steadham, E. Huff-Lonergan, S. Lonergan","doi":"10.22175/MMB.12408","DOIUrl":"https://doi.org/10.22175/MMB.12408","url":null,"abstract":"Fresh meat quality is adversely affected by protein oxidation. However, a fundamental understanding of the diverse factors that influence protein oxidation in postmortem muscle remains elusive. Peroxiredoxin-2 (Prdx2), an antioxidant protein, is more abundant in tough meat based on instrumental tenderness; however, the role of Prdx2 in postmortem skeletal muscle is unknown. Therefore, the objective was to develop a method to purify Prdx2 from the diaphragm, psoas major, and longissimus lumborum. Proteins soluble at low ionic strength were extracted, dialyzed, clarified, and loaded onto a Q-Sepharose anion exchange column equilibrated with TEM (pH 7.4). In all preparations, Prdx2 eluted between about 75 and 115 mM NaCl. Immunoreactive fractions were dialyzed against TEM (pH 8.0), clarified, and loaded onto a DEAE-650S anion exchange column. In all preparations, Prdx2 eluted between approximately 55 and 75 mM NaCl. Immunoreactive fractions were concentrated and loaded onto a Superose-12 size exclusion column. Prdx2 was detected between 14 and 16 mL, and these fractions were concentrated and reduced with 0.5% 2-mercaptoethanol. A final pass over the Superose-12 column was conducted, and Prdx2 was detected in 2 peaks from 11–12 mL and 15–16 mL. Fractions 15–16 were pooled and retained for further experiments. The elution profile of Prdx2 in all 3 muscles was similar. The iden- tification of the primary protein was confirmed with liquid chromatography with tandem mass spectrometry. The purity of Prdx2 off the final Superose-12 column was approximately 33%, 52%, and 47% pure in the diaphragm, psoas major, and longissimus lumborum, respectively. This is the first report of a method to partially purify Prdx2 from skeletal muscle.","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74325726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Characterization of Carcass Color Differences Between Hens (Small Birds) and Meat-Type Male Pheasants (Large Birds) 母鸡(小型鸟类)与肉型雄性野鸡(大型鸟类)胴体颜色差异的表征
Meat and Muscle Biology Pub Date : 2021-04-13 DOI: 10.22175/MMB.11589
J. Claus, K. Hwang
{"title":"Characterization of Carcass Color Differences Between Hens (Small Birds) and Meat-Type Male Pheasants (Large Birds)","authors":"J. Claus, K. Hwang","doi":"10.22175/MMB.11589","DOIUrl":"https://doi.org/10.22175/MMB.11589","url":null,"abstract":"The underlying changes in hen carcass color upon freezing were compared with the color of meat-type male pheasants upon freezing. Chemical and physical assessments of these two pheasant types (n=5) and the effects of different chilling methods on hen carcasses (n=10) were evaluated. The results showed that hen carcasses exhibited more red pigmentation (myoglobin, hemoglobin), as well as significantly higher pH values and redness, than the carcasses from meat-type pheasants. The moisture content was higher in hens than in meat-type pheasants, especially in the skin. The intermediate fiber (IIA) type was the only type found in the pectoralis major muscle, regardless of pheasant type. Chilling method significantly changed the color attributes of the hen carcass. Immersion chilling decreased skin redness (less pigmentation and Commission Internationale de l ́Eclairage [CIE] a*); the breast meat was less red than that from the chilling-in-a-bag condition. The skin had substantially higher levels of red pigmentation than the breast muscles, irrespective of the pheasant type and chilling method (P < 0.05). Our findings suggest that the more intense red appearance may be related to a combination of greater residual hemoglobin levels and higher pH within the skin. The greater moisture content of the skin may have facilitated the development of greater transparency to the darker, more red breast muscle.","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"121 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78437972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Dry-Heat Cookery Method on Volatile Flavor Compound Development and Consumer Evaluation of Six Beef Muscles 干热烹饪法对六种牛肉肌肉挥发性风味化合物开发及消费者评价的评价
Meat and Muscle Biology Pub Date : 2021-02-25 DOI: 10.22175/MMB.11710
K. Vierck, J. Legako, J. Brooks
{"title":"Evaluation of Dry-Heat Cookery Method on Volatile Flavor Compound Development and Consumer Evaluation of Six Beef Muscles","authors":"K. Vierck, J. Legako, J. Brooks","doi":"10.22175/MMB.11710","DOIUrl":"https://doi.org/10.22175/MMB.11710","url":null,"abstract":"The objective of this study was to determine the influence of dry-heat cookery on beef flavor development of multiple beef muscles. Beef strip loins, top sirloin butts, paired tenderloins, paired shoulder clods, and chuck rolls were collected from USDA Low Choice carcasses (Small00–Small100 marbling; N = 20). Subprimals were wet aged in the absence of light for 21 d at 0°C to 4°C. Subprimals were fabricated into 2.54-cm-thick steaks representative of the following muscles: Gluteus medius, Infraspinatus, Longissimus lumborum, Psoas major, Serratus ventralis, and Triceps brachii and randomly assigned to one of 4 dry-heat cookery methods: charbroiler grill (CHAR), clamshell grill (CLAM), convection oven (OVEN), and salamander broiler (SALA). Steaks were cooked to a medium degree of doneness (71°C) on the randomly assigned cooking method. Untrained consumer panelists (N = 300) evaluated each sample for flavor, tenderness, juiciness, and overall liking. No interactions were observed between cooking method and muscle (P ≥ 0.344) for any palatability traits evaluated. Consumers preferred CHAR steaks (P < 0.05) over CLAM steaks for flavor, tenderness, juiciness, and overall liking. Additionally, CLAM steaks were rated lower (P < 0.05) than all other methods for tenderness and juiciness. OVEN and SALA steaks were rated higher (P < 0.05) than CLAM steaks by consumers for tenderness and juiciness but were similar (P > 0.05) to CLAM steaks for overall liking. CHAR steaks produced a greater concentration of Maillard compounds compared with the other cooking methods. Steaks cooked using OVEN and SALA (P < 0.05) produced more lipid oxidation products. Additionally, CHAR steaks produced the greatest (P < 0.05) total volatile production compared with all other treatments, which may be a result of the combination of Maillard reaction products and the lipid degradation products.","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"91 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83342304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Effects of dry heat cookery method on beef strip loin steaks of two quality grades following sous vide preparation 干热烹调方法对真空烹调后两种品质等级牛里脊牛排的影响
Meat and Muscle Biology Pub Date : 2021-02-19 DOI: 10.22175/MMB.11700
J. Brooks, J. Legako, K. Vierck
{"title":"Effects of dry heat cookery method on beef strip loin steaks of two quality grades following sous vide preparation","authors":"J. Brooks, J. Legako, K. Vierck","doi":"10.22175/MMB.11700","DOIUrl":"https://doi.org/10.22175/MMB.11700","url":null,"abstract":"The objective of this study was to determine the influence of dry-heat cookery method on beef flavor development in strip loin steaks from 2 USDA quality grades following sous vide preparation. Beef strip loins were selected from 2 USDA quality grades: upper 2/3 Choice (Modest00–Moderate100) and Select (Slight00–Slight100 marbling, n= 20/grade). Following 21 d of wet aging, strip loins were fabricated into 2.54-cm thick-steaks and randomly assigned to one of 4 dryheat cookery methods: charbroiler grill (CHAR), clamshell grill (CLAM), convection oven (OVEN), and salamander broiler (SALA). Prior to untrained consumer panel and volatile compound analysis via gas chromatography-mass spectrometry, steaks were cooked under sous vide conditions for 1.5 h, then finished on the assigned cookery method. There were no cooking method × quality grade interactions (P≥ 0.076) for all consumer traits evaluated. Overall, SALA steaks received higher (P< 0.05) ratings by consumers than CLAM steaks for all palatability traits. OVEN steaks had greater scores (P< 0.05) than CLAM steaks for juiciness, tenderness, and overall liking but were similar to CLAM steaks (P> 0.05) for flavor. CHAR steaks were similar (P> 0.05) to CLAM steaks for flavor but were rated higher (P< 0.05) for tenderness, juiciness, and overall liking. Steaks cooked using the OVEN method produced a greater concentration of lipid-derived volatiles, such as alcohols, aldehydes, and carboxylic acids. In direct contrast, CHAR steaks produced a higher concentration of pyrazines and Strecker aldehydes, which are derived from theMaillard reaction. These data indicate that cookery method, and therefore heat transfer method, has a substantially stronger influence on consumer ratings and flavor development than USDA quality grade in this study when steaks are prepared using sous vide methods.","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81681761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Contribution of Early-Postmortem Proteome and Metabolome to Ultimate pH and Pork Quality 死后早期蛋白质组和代谢组对最终pH值和猪肉品质的贡献
Meat and Muscle Biology Pub Date : 2021-01-26 DOI: 10.22175/MMB.11709
E. Zuber, Amanda C. Outhouse, E. Helm, N. Gabler, K. Prusa, E. Steadham, E. Huff-Lonergan, S. Lonergan
{"title":"Contribution of Early-Postmortem Proteome and Metabolome to Ultimate pH and Pork Quality","authors":"E. Zuber, Amanda C. Outhouse, E. Helm, N. Gabler, K. Prusa, E. Steadham, E. Huff-Lonergan, S. Lonergan","doi":"10.22175/MMB.11709","DOIUrl":"https://doi.org/10.22175/MMB.11709","url":null,"abstract":"This study's objectives were to identify how subtle differences in ultimate pH relate to differences in pork quality and to understand how early-postmortem glycolysis contributes to variation in ultimate pH. The hypothesis was that elements in early-postmortem longissimus thoracis et lumborum proteome and metabolome could be used to predict quality defects associated with pH decline. Temperature and pH of the longissimus thoracis et lumborum were measured at 45 min, 24 h, and 14 d postmortem. Quality measurements were made after 14 d of aging. Groups were classified as normal pH (NpH; x̄ = 5 . 59 [5.53–5.67]; NpH, n = 10) and low pH (LpH; x̄ = 5 . 42 [5.38–5.45]; LpH, n = 10) at 14 d postmortem. Metabolites from 45 min postmortem were identified using GC-MS. Relative differences between proteins were quantified with two-dimensional difference in gel electrophoreses, and spots were identified with MALDI-MS. Western blot analyses were used to measure phosphofructokinase, peroxiredoxin-2, and reduced and non-reduced adenosine monophosphate deaminase-2 at 45 min and 14 d postmortem. Ultimate pH classification did not affect 45-min-postmortem pH (P = 0.64); 14-d pH was different between groups (P < 0.01). NpH had less purge loss (P < 0.01), was darker (P < 0.01), had lower star probe (P < 0.01), and had less intact day-7 desmin (P = 0.02). More pyruvate (P = 0.01) and less lactate (P = 0.09) was observed in NpH, along with more soluble lactate dehydrogenase (P = 0.03) and pyruvate kinase (P < 0.10). These observations indicate that differences in enzyme abundance or solubility may produce more pyruvate and less lactate. Fructose 6-phosphate was more abundant (P = 0.08) in the LpH group, indicating that phosphofructokinase may be involved in glycolytic differences. Furthermore, greater abundance of heat shock proteins, peroxiredoxin-2 (P = 0.02), and malate (P = 0.01) early postmortem all suggest differences in mitochondrial function and oxidative stability that contribute to quality differences. These results show that even subtle changes in ultimate pH can influence pork quality. The proteome and metabolome at 45 min postmortem are associated with variation in the extent of pH decline.","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85372354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Myoglobin Post-Translational Modifications Influence Color Stability of Beef Longissimus Lumborum 肌红蛋白翻译后修饰影响牛肉腰最长肌颜色稳定性
Meat and Muscle Biology Pub Date : 2021-01-21 DOI: 10.22175/MMB.11689
Yifei Wang, Shuting Li, G. Rentfrow, Jing Chen, Haining Zhu, S. Suman
{"title":"Myoglobin Post-Translational Modifications Influence Color Stability of Beef Longissimus Lumborum","authors":"Yifei Wang, Shuting Li, G. Rentfrow, Jing Chen, Haining Zhu, S. Suman","doi":"10.22175/MMB.11689","DOIUrl":"https://doi.org/10.22175/MMB.11689","url":null,"abstract":"Post-translational modifications (PTM) of proteins play critical roles in biological processes. PTM of muscle proteins influence meat quality. Nonetheless, myoglobin (Mb) PTM and their impact on fresh beef color stability have not been characterized yet. Therefore, our objectives were to identify Mb PTM in beef longissimus lumborum muscle during postmortem aging and to characterize their influence on color stability. The longissimus lumborum muscles from 9 (n = 9) beef carcasses (24 h postmortem) were subjected to wet aging for 0, 7, 14, and 21 d. At the end of each wet-aging period, steaks were fabricated. One steak for analyses of PTM was immediately frozen at −80°C, whereas other steaks were assigned to refrigerated storage in the darkness under aerobic packaging. Instrumental color and biochemical attributes were evaluated on day 0, 3, or 6 of storage. Mb PTM were analyzed using two-dimensional electrophoresis and tandem mass spectrometry. Surface redness (a* value), color stability, and Mb concentration decreased (P < 0.05) upon aging. Gel image analyses identified 6 Mb spots with similar molecular weight (17 kDa) but different isoelectric pH. Tandem mass spectrometry identified multiple PTM (phosphorylation, methylation, carboxymethylation, acetylation, and 4-hydroxynonenal alkylation) in these 6 isoforms. The amino acids susceptible to phosphorylation were serine (S), threonine (T), and tyrosine, whereas other PTM were detected in lysine (K), arginine (R), and histidine residues. Additionally, distal histidine (position 64), critical to heme stability, was found to be alkylated. Overall, Mb PTM increased with aging. The aging-induced PTM, especially those occurring close to hydrophobic heme pocket, could disrupt Mb tertiary structure, influence heme affinity, and compromise oxygen binding capacity, leading to decreased color stability of fresh beef. Furthermore, PTM at K45, K47, and K87 were unique to Mb from non-aged beef, whereas PTM at R31, T51, K96, K98, S121, R139, and K147 were unique to Mb from aged counterparts, indicating that these Mb PTM could be used as novel biomarkers for fresh beef color stability.","PeriodicalId":18316,"journal":{"name":"Meat and Muscle Biology","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88215043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信