Terra C. Hiebert, Anne E. Aasjord, Daniel M. Chourrout, Anne W. Thompson, Kelly R. Sutherland
{"title":"Prey particle surface property mediates differential selection by the ubiquitous appendicularian Oikopleura dioica","authors":"Terra C. Hiebert, Anne E. Aasjord, Daniel M. Chourrout, Anne W. Thompson, Kelly R. Sutherland","doi":"10.1002/lno.12819","DOIUrl":"10.1002/lno.12819","url":null,"abstract":"<p>Cell surface properties can strongly mediate microbial interactions with predators in soil and host-pathogen systems. Yet, the role of microbial surface properties in avoiding or enhancing predation in the ocean is less well known. Appendicularians are globally abundant marine suspension feeders that capture marine microorganisms in a complex mucous filtration system. We used artificial microspheres to test whether the surface properties of prey particles influenced selection by the appendicularian, <i>Oikopleura dioica</i>. We used a range of microsphere sizes (0.5, 1, 2, and 3 <i>μ</i>m), concentrations (~ 10<sup>3</sup>–10<sup>6</sup> particles mL<sup>−1</sup>), and two charges (amine-modified, more positive vs. carboxylate-modified, more negative) to represent open-ocean microbial communities. We found that appendicularians selected between the particles of different charge. More negatively charged particles were enriched in the gut by up to 3.8-fold, while more positive particles were enriched in the mucous filters by up to 4.7-fold, leading to different particle fates. These results expand understanding of the mechanisms by which filter-feeders select between prey and reveal a mechanism by which marine bacteria could rapidly alter their susceptibility to predation, either through adaption or acclimation.</p>","PeriodicalId":18143,"journal":{"name":"Limnology and Oceanography","volume":"70 4","pages":"977-988"},"PeriodicalIF":3.8,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143258604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xinyu Sun, Kendra S. Cheruvelil, Patrick J. Hanly, Patricia A. Soranno
{"title":"Lake chlorophyll responses to drought are related to lake type, connectivity, and ecological context across the conterminous United States","authors":"Xinyu Sun, Kendra S. Cheruvelil, Patrick J. Hanly, Patricia A. Soranno","doi":"10.1002/lno.12817","DOIUrl":"10.1002/lno.12817","url":null,"abstract":"<p>Local and regional-scaled studies point to the important role of lake type (natural lakes vs. reservoirs), surface water connectivity, and ecological context (multi-scaled natural settings and human factors) in mediating lake responses to disturbances like drought. However, we lack an understanding at the macroscale that incorporates multiple scales (lake, watershed, region) and a variety of ecological contexts. Therefore, we used data from the LAGOS-US research platform and applied a local water year timeframe to 62,927 US natural lakes and reservoirs across 17 ecoregions to examine how chlorophyll <i>a</i> responds to drought across various ecological contexts. We evaluated chlorophyll <i>a</i> changes relative to each lake's baseline and drought year. Drought led to lower and higher chlorophyll <i>a</i> in 18% and 20%, respectively, of lakes (both natural lakes and reservoirs included). Natural lakes had higher magnitudes of change and probabilities of increasing chlorophyll <i>a</i> during droughts than reservoirs, and these differences were particularly pronounced in isolated and highly-connected lakes. Drought responses were also related to long-term average lake chlorophyll <i>a</i> in complex ways, with a positive correlation in less productive lakes and a negative correlation in more productive lakes, and more pronounced drought responses in higher-productivity lakes than lower-productivity lakes. Thus, lake chlorophyll responses to drought are related to interactions between lake type and surface connectivity, long-term average chlorophyll <i>a</i>, and many other multi-scaled ecological factors (e.g., soil erodibility, minimum air temperature). These results reinforce the importance of integrating multi-scaled ecological context to determine and predict the impacts of global changes on lakes.</p>","PeriodicalId":18143,"journal":{"name":"Limnology and Oceanography","volume":"70 4","pages":"941-958"},"PeriodicalIF":3.8,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lno.12817","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143192063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Baroclinic instability-induced intensification of phytoplankton blooms at submesoscales in eutrophic frontal regions","authors":"Zhonghua Zhao, Lie-Yauw Oey, Zhaoyun Chen, Bangqin Huang, Huijie Xue, Shuh-Ji Kao, Feng Cai, Yuwu Jiang","doi":"10.1002/lno.12816","DOIUrl":"10.1002/lno.12816","url":null,"abstract":"<p>Off-coast phytoplankton blooms occur frequently in the frontal region of the eutrophic Taiwan Strait during the northeasterly monsoon relaxation period, as consistently revealed by extensive cruise and satellite observations. Realistic model simulations have shown that restratification by frontal baroclinic instability (BCI) plays a crucial role in triggering blooms under nutrient-rich conditions. This study deciphered the distinct contributions of submesoscale and mesoscale BCIs to bloom development using sensitivity tests of an idealized model of the Taiwan Strait featuring an intense alongshore front with ample nutrients. In three-dimensional fine simulations with both submesoscale and mesoscale BCIs present, blooms were triggered by the cessation of a down-front wind. Chlorophyll <i>a</i> was higher in submesoscale front regions than in mesoscale regions, primarily because of the higher upper-ocean stability resulting from more effective restratification by submesoscale BCI. In three-dimensional coarse simulations, mesoscale BCI led to relatively lower upper-ocean stability and weaker blooms following wind relaxation, consistent with those in mesoscale regions in corresponding three-dimensional fine simulations. In two-dimensional simulations without submesoscale and mesoscale BCIs, blooms could not be triggered despite the cessation of a down-front wind, primarily because of the absence of significant near-surface restratification by BCIs. Furthermore, although symmetric instability was present in two-dimensional fine simulations, its contribution to blooms was limited because of its minimal restratification effect. These results show that BCIs play the predominant role in triggering off-coast blooms in eutrophic coastal front regions such as the Taiwan Strait.</p>","PeriodicalId":18143,"journal":{"name":"Limnology and Oceanography","volume":"70 4","pages":"925-940"},"PeriodicalIF":3.8,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143192064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Robert L. Iles, Steven F. DiMarco, Gerardo Gold‐Bouchot, Anthony H. Knap
{"title":"Diagnosing coastal processes using machine learning and ocean buoyancy gliders","authors":"Robert L. Iles, Steven F. DiMarco, Gerardo Gold‐Bouchot, Anthony H. Knap","doi":"10.1002/lno.12810","DOIUrl":"https://doi.org/10.1002/lno.12810","url":null,"abstract":"Ocean buoyancy gliders provide a comprehensive view of the water column, offering more than simply a snapshot of a single moment in time or space. In this study, we applied the established machine learning method, k‐means clustering, to a glider dataset collected in the summer of 2015 in the northern Gulf of Mexico. Clustering analysis of chromophoric dissolved organic matter and salinity revealed the physical structure of water masses, both vertically within the water column and horizontally along the shelf. Supplementary statistical analyses, including principal component analysis and ANOVA, of individual clusters confirmed the clusters were statistically distinct from one another and provided insights into the factors contributing to their differentiation. The clusters identified in the glider dataset represent water masses variously distinguished by river plumes, wind‐induced upwelling effects, shifts in currents, density‐induced stratification, and biological processes. This study demonstrates that applying machine learning clustering methods to subsurface glider data is a novel technique that enhances the analytical capabilities of both glider and other oceanographic datasets.","PeriodicalId":18143,"journal":{"name":"Limnology and Oceanography","volume":"40 1","pages":""},"PeriodicalIF":4.5,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143192073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nan Wu, Stuart W. D. Grieve, Andrew J. Manning, Kate L. Spencer
{"title":"Marine snow as vectors for microplastic transport: Multiple aggregation cycles account for the settling of buoyant microplastics to deep-sea sediments","authors":"Nan Wu, Stuart W. D. Grieve, Andrew J. Manning, Kate L. Spencer","doi":"10.1002/lno.12814","DOIUrl":"10.1002/lno.12814","url":null,"abstract":"<p>Many studies have reported the paradoxical observation of high concentrations of low-density microplastics (plastic particles < 5 mm) in deep-sea sediments despite their buoyancy. The incorporation of buoyant microplastics into marine snow has been observed to enhance microplastic settling. Previous studies on the vertical movement of buoyant microplastics have been unable to theoretically account for these ocean observations and no study has comprehensively elucidated microplastic transport pathways in the ocean from the surface to seafloor. Here, we establish a one-dimensional theoretical model, that embraces key elements of the flocculation process, to explain how marine snow acts as a vector to transport buoyant microplastics to deep water and the ocean bottom. Microplastics reach the ocean floor through multiple cycles of aggregation, settling, and disaggregation between marine snow and microplastics. Each settling cycle results in a net settling of 200–400 m. We demonstrate that microplastics with different sizes show distinct vertical settling behaviors and only microplastics less than 100 <i>μ</i>m in diameter can reach the ocean bottom. This theoretical model refines our ability to predict and understand the global and long-term fate, transport, and inventory of microplastics in the ocean interior, the influence of microplastics on the biological carbon pump and the efficacy of plastic management policies.</p>","PeriodicalId":18143,"journal":{"name":"Limnology and Oceanography","volume":"70 4","pages":"899-910"},"PeriodicalIF":3.8,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lno.12814","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143192065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Juan Gerardo Gutiérrez-Bravo, Mark A. Altabet, Laura Sánchez-Velasco, Sylvia Patricia Adelheid Jiménez-Rosenberg
{"title":"Midwater anoxia disrupts the trophic structure of zooplankton and fish in an oxygen deficient zone","authors":"Juan Gerardo Gutiérrez-Bravo, Mark A. Altabet, Laura Sánchez-Velasco, Sylvia Patricia Adelheid Jiménez-Rosenberg","doi":"10.1002/lno.12813","DOIUrl":"10.1002/lno.12813","url":null,"abstract":"<p>Anoxic waters in the ocean's oxygen deficient zones (ODZs) limit the vertical migrations of zooplankton and mesopelagic fish impacting their ecology and influence on biogeochemical processes. Using an oxypleth-tracking, nighttime-only sampling protocol, this research reconstructed the trophic interactions of fish larvae and adults, and zooplankton, across the Eastern Tropical North Pacific ODZ. Bulk zooplankton δ<sup>15</sup>N increased latitudinally by ~ 3.3‰ from Costa Rica to Baja California due to anoxia-derived denitrification and consequent enrichment of nitrogen sources for producers. Zooplankton δ<sup>15</sup>N also increased with depth, with an abrupt 3.4‰ increase below the anoxic core (~ 900 m depth), indicating a distinct trophic structure in the resident zooplankton community. Above the anoxic core, δ<sup>15</sup>N was similar for fish larvae (10.1‰) and zooplankton (10.5‰), reflecting a shared food source. An exception was the hypoxia-tolerant myctophid <i>Diogenichthys laternatus</i> (δ<sup>15</sup>N = 7.5‰) that possibly feeds on chemoautotrophy-derived material at the oxic-anoxic interface. The δ<sup>15</sup>N of fish adults residing below the anoxic core, like the meso-bathypelagic <i>Notolychnus valdiviae</i> (17.11‰) and <i>Cyclothone</i> spp. (15.89‰), was, on average, 4.8‰ higher than larval stages sampled at shallower depths, and 1.2‰ higher than zooplankton below the anoxic core. This stark increase in fish and zooplankton δ<sup>15</sup>N directly below the anoxic core suggests that anoxic waters act as a barrier for the downward trophic transfer by vertical migrants into the deep sea. Considering the current trends of ocean deoxygenation, this anoxia-derived disruption of the migrant pump could limit the carbon sequestration potential of ODZs.</p>","PeriodicalId":18143,"journal":{"name":"Limnology and Oceanography","volume":"70 4","pages":"886-898"},"PeriodicalIF":3.8,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143192066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jonas Brünjes, Florence Schubotz, Andreas Teske, Michael Seidel
{"title":"Molecular composition of dissolved organic matter from young organic-rich hydrothermal deep-sea sediments","authors":"Jonas Brünjes, Florence Schubotz, Andreas Teske, Michael Seidel","doi":"10.1002/lno.12812","DOIUrl":"10.1002/lno.12812","url":null,"abstract":"<p>Hydrothermal transformations of dissolved organic matter (DOM) are governed by temperature and sedimentary organic carbon content, resulting in the release of hydrothermal DOM containing bioavailable compounds fueling benthic microbes. However, the temperature-dependent molecular changes in porewater DOM from organic-rich hydrothermal sediments, and the extent to which these changes contribute to the marine recalcitrant DOM, remain largely unexplored. Here we investigated the DOM composition of hydrothermal porewater and bottom water samples from the Guaymas Basin, Gulf of California, where basaltic sill intrusions generate hydrothermal petroleum in organic-rich sediments. Samples containing hydrothermal petroleum with in situ temperatures from 4°C to > 106°C were analyzed using Fourier-transform ion cyclotron resonance mass spectrometry and parallel factor analysis of excitation-emission matrices from fluorescent DOM (FDOM). We found that the porewater DOM composition was strongly influenced by temperature and petroleum dissolution, evidenced by the enrichment of hydrothermal DOM with highly unsaturated, oxygen-depleted aromatic, sulfur-containing molecular formulae and petroleum-associated FDOM compared to a cold reference site. In bottom waters, hydrothermal DOM accounted for ~ 26% of the DOM molecular formulae, with 82% exhibiting hydrogen-to-carbon ratios < 1.5, indicating their recalcitrance. The remaining ~ 18% of the hydrothermal molecular formulae were aliphatic and saturated, representing the release of bioavailable DOM to the ocean. Our results show that hydrothermal sediments are a source of both bioavailable and recalcitrant DOM, releasing water-soluble petroleum-derived compounds to the deep ocean. Our study highlights the need for more quantitative research on the contribution of hydrothermal sediments to deep-sea DOM cycling.</p>","PeriodicalId":18143,"journal":{"name":"Limnology and Oceanography","volume":"70 4","pages":"870-885"},"PeriodicalIF":3.8,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lno.12812","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143125198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gabriela Dangl, Claudia Frey, Christiane Hassenrück, Bita Sabbaghzadeh, Janine Wäge-Recchioni, Moritz F. Lehmann, Heide Schulz-Vogt, Jenny Fabian, Martin Ley, Gregor Rehder, Klaus Jürgens
{"title":"Processes and microorganisms driving nitrous oxide production in the Benguela Upwelling System","authors":"Gabriela Dangl, Claudia Frey, Christiane Hassenrück, Bita Sabbaghzadeh, Janine Wäge-Recchioni, Moritz F. Lehmann, Heide Schulz-Vogt, Jenny Fabian, Martin Ley, Gregor Rehder, Klaus Jürgens","doi":"10.1002/lno.12811","DOIUrl":"10.1002/lno.12811","url":null,"abstract":"<p>Upwelling systems and their associated oxygen deficient zones (ODZs) are hotspots of nitrous oxide (N<sub>2</sub>O) production in the ocean. The Benguela Upwelling System (BUS) is a highly productive region and an important, yet variable source of N<sub>2</sub>O to the atmosphere. This study examined underlying processes and microbial key players governing N<sub>2</sub>O production in the BUS during the austral winter. <sup>15</sup>N-tracer incubation experiments were conducted to track N<sub>2</sub>O production from NH<sub>4</sub><sup>+</sup> oxidation and denitrification. N<sub>2</sub>O production and consumption mechanisms over a longer temporal scale were determined through natural-abundance isotope analyses. Metagenomics and 16S rRNA gene amplicon sequencing were used to identify potential key prokaryotes driving N<sub>2</sub>O production. Our results showed that, compared with permanent ODZs, the BUS is characterized by a higher oxidative and a lower reductive N<sub>2</sub>O production, both of which exhibit substantial spatial variability. N<sub>2</sub>O production peaked in low-oxygen (O<sub>2</sub>) waters, with nearly equal contributions of oxidative and reductive processes, suggesting their co-occurrence across an O<sub>2</sub> concentration range broader than previously thought. However, the observed N<sub>2</sub>O isotope signatures implied a legacy of recent and extensive N<sub>2</sub>O reduction to N<sub>2</sub>. Metagenomic and 16S rRNA gene data identified denitrifiers belonging to Thioglobaceae and the archaeal ammonia-oxidizers Nitrosopumilaceae among the potential key drivers of N<sub>2</sub>O production. Our study provides a comprehensive picture of N<sub>2</sub>O production in the BUS, revealing significant variability in the N-cycling regime and underlying N<sub>2</sub>O production mechanisms, and demonstrating the value of combining direct rate measurements with more integrative approaches, such as molecular omics and natural-abundance stable isotope tracers.</p>","PeriodicalId":18143,"journal":{"name":"Limnology and Oceanography","volume":"70 4","pages":"850-869"},"PeriodicalIF":3.8,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lno.12811","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143084159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jennifer J. Freer, Victoria Warwick-Evans, Georg Skaret, Bjørn A. Krafft, Sophie Fielding, Philip N. Trathan
{"title":"A new dynamic distribution model for Antarctic krill reveals interactions with their environment, predators, and the commercial fishery in the south Scotia Sea region","authors":"Jennifer J. Freer, Victoria Warwick-Evans, Georg Skaret, Bjørn A. Krafft, Sophie Fielding, Philip N. Trathan","doi":"10.1002/lno.12809","DOIUrl":"10.1002/lno.12809","url":null,"abstract":"<p>The management strategy for the Antarctic krill (<i>Euphausia superba</i>) fishery is being revised. A key aim is to spatially and temporally allocate catches in a manner that minimizes impacts to both the krill stock and dependent predators. This process requires spatial information on the distribution and abundance of krill, yet gaps exist for an important fishing area surrounding the South Orkney Islands in the south Scotia Sea. To fill this need, we create a dynamic distribution model for krill in this region. We used data from a spatially and temporally consistent acoustic survey (2011–2020) and year-specific environmental covariates within a two-part hurdle model. The model successfully captured observed spatial and temporal patterns in krill density. The covariates found to be most important included distance from shelf break, distance from summer sea ice extent, and salinity. The northern and eastern shelf edges of the South Orkney Islands were areas of consistently high krill density and displayed strong spatial overlap between intense fishing activity and foraging chinstrap penguins. High mean krill density was also linked to oceanographic features located within the Weddell Sea. Our data suggest that years in which these features were closer to the South Orkney shelf were also years of positive Southern Annular Mode and higher observed krill densities. Our findings highlight existing fishery–predator–prey overlap in the region and support the hypothesis that Weddell Sea oceanography may play a role in transporting krill into this region. These results will feed into the next phase of krill fisheries management assessment.</p>","PeriodicalId":18143,"journal":{"name":"Limnology and Oceanography","volume":"70 4","pages":"833-849"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lno.12809","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Limited degradability of dissolved organic carbon, nitrogen, and phosphorus during contrasting seasons in a tropical coastal environment","authors":"Jiangyong Chu, Christian Lønborg, Patrick Martin","doi":"10.1002/lno.12803","DOIUrl":"10.1002/lno.12803","url":null,"abstract":"<p>The biogeochemistry of dissolved organic matter (DOM) is poorly understood in tropical coastal waters. Here, we quantified the biological and photochemical lability of dissolved organic carbon, nitrogen, and phosphorus, in the tropical coastal waters of Singapore. We conducted experiments during the inter-monsoon, the mid-southwest monsoon, and the late southwest monsoon seasons, which span the greatest range of biogeochemical conditions found in the area. The DOM lability was quantified as concentration changes during 90-d biodegradation and 7-d photoreactor incubations. Overall, DOM showed low lability, even though dissolved organic nitrogen and dissolved organic phosphorus accounted for most of the dissolved nitrogen and phosphorus. In the biodegradation experiments, only 5–15% of dissolved organic carbon, 0–7% of dissolved organic nitrogen, and 8–21% of dissolved organic phosphorus were degraded. The addition of labile dissolved organic carbon, intended to test priming effects and to ensure the microbes were not carbon-limited, had no measurable impact on the results. During our photochemical experiments only 2–10% of the dissolved organic carbon were degraded, while neither dissolved organic nitrogen nor dissolved organic phosphorus showed consistent photochemical losses. The DOM optical properties (absorbance and fluorescence spectra) showed limited or no changes during the biodegradation experiments but larger declines in the photochemical experiments. Overall, the biodegradation of DOM was highest during the inter-monsoon, when autochthonous DOM was most dominant, while photolability was greater during the terrestrial DOM-rich southwest monsoon. Our results illustrate that in some tropical coastal environments, DOM can be fairly resistant to biological and photochemical degradation, and thus does not represent a large stock of potentially available nutrients.</p>","PeriodicalId":18143,"journal":{"name":"Limnology and Oceanography","volume":"70 3","pages":"775-791"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lno.12803","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143072424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}