Lymphokine research最新文献_第5页

V-abl confers resistance and growth advantage to TNF-alpha in NIH3T3 cells. V-abl赋予NIH3T3细胞对tnf - α的抗性和生长优势。

Lymphokine research Pub Date : 1990-01-01

T C Suen, R U Rodriguez, M C Hung, J Klostergaard

{"title":"V-abl confers resistance and growth advantage to TNF-alpha in NIH3T3 cells.","authors":"T C Suen, R U Rodriguez, M C Hung, J Klostergaard","doi":"","DOIUrl":"","url":null,"abstract":"Tumor necrosis factor (TNF-alpha) is a cytokine produced by macrophages and monocytes, and has been shown to have cytolytic, cytostatic or growth-stimulatory activity on transformed cells. However, the mechanism of these growth modulating activities of TNF-alpha is unknown. By studying the response of different oncogene-transformed NIH3T3 cells to TNF-alpha, we showed that the oncogene v-abl confers resistance to the cytostatic and cytolytic activities on TNF-alpha compared to the parental NIH3T3 cells. Most interestingly, v-abl expression also resulted in a growth-enhancing response to TNF-alpha at up to the highest dose of 6,400 units/ml. These altered properties were not due to the transformation event itself, since EJ-ras oncogene transformed NIH3T3 cells were more susceptible to TNF-alpha than the parental cells. Moreover, EMT-6, a mouse adenocarcinoma cell line, which responded similarly to NIH3T3 cells, did not show growth-enhancement at high TNF-alpha dosages. Though resistant to the direct cytotoxic activity of TNF-alpha, the v-abl transformed cell line was effectively killed by macrophages, as were the other cell lines. This suggests tumor cell killing by macrophages must involve mechanisms in addition to the secretion of TNF-alpha.","PeriodicalId":18130,"journal":{"name":"Lymphokine research","volume":"9 1","pages":"15-26"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13335170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of interleukin 1 beta gene expression using antisense phosphorothioate oligonucleotides. 利用反义硫代寡核苷酸调节白细胞介素1 β基因表达。

Lymphokine research Pub Date : 1990-01-01

J Manson, T Brown, G Duff

引用次数: 0

Development and characterization of a lyophilized dosage form of IL-1 beta. 白介素-1 β冻干剂型的研制和表征。

Lymphokine research Pub Date : 1990-01-01

G C Visor, K P Tsai, M D Miller, J Duffy, T Calderwood, D Lokensgard, J Killian, T Malefyt, L Gu, I Massey

引用次数: 0

Effect of stress-related hormones on macrophage receptors and response to tumor necrosis factor. 应激相关激素对巨噬细胞受体及肿瘤坏死因子反应的影响。

Lymphokine research Pub Date : 1990-01-01

L E Bermudez, M Wu, L S Young

引用次数: 0

Tolerance to endotoxin in vitro: independent regulation of interleukin-1, tumor necrosis factor and interferon alpha production during in vitro differentiation of human monocytes. 体外对内毒素的耐受:人单核细胞体外分化过程中白细胞介素-1、肿瘤坏死因子和干扰素α产生的独立调节。

Lymphokine research Pub Date : 1990-01-01

J L Lepe-Zuniga, J Klostergaard

{"title":"Tolerance to endotoxin in vitro: independent regulation of interleukin-1, tumor necrosis factor and interferon alpha production during in vitro differentiation of human monocytes.","authors":"J L Lepe-Zuniga, J Klostergaard","doi":"","DOIUrl":"","url":null,"abstract":"Bacterial lipopolysaccharide (LPS)-induced production of three known endogenous pyrogens, interferon alpha (IFN-alpha), interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF-alpha) was studied during in vitro differentiation of human peripheral blood monocytes. In freshly seeded cells, secretion of IL-1 and TNF-alpha, but not IFN-alpha were readily induced by LPS. After 24 hr and for up to 14 days of culture, monocytes became irreversibly tolerant to LPS for the release of IL-1. IFN-alpha secretion was not induced by LPS in cells cultured for up to 8 days. Monocytes also became tolerant to LPS for TNF-alpha production 48 hr after an initial stimulation with LPS. This tolerant state, however, was transient, lasting from 6 to 8 days, after which competence for TNF secretion resumed. These observations demonstrate that regulation of production of IL-1, TNF-alpha and IFN-alpha by human mononuclear phagocytes is mutually independent, related to the stage of cell differentiation and modulated by cell stimulation. Since in vitro tolerance to LPS mimics the in vivo tolerance to LPS with respect to fever, we speculate that they are closely related.","PeriodicalId":18130,"journal":{"name":"Lymphokine research","volume":"9 3","pages":"309-19"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13356926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytokine effects on the down regulation of cytolytic T cell responses in vitro. 细胞因子对体外细胞溶解性T细胞反应下调的影响。

Lymphokine research Pub Date : 1990-01-01

C Terajima, A Koontz, M Kelley, D R Webb, B H Devens

{"title":"Cytokine effects on the down regulation of cytolytic T cell responses in vitro.","authors":"C Terajima, A Koontz, M Kelley, D R Webb, B H Devens","doi":"","DOIUrl":"","url":null,"abstract":"Lymphocytes from mouse and men can be stimulated by a variety of cellular signals including concanavalin A and the interferons to generate cells capable of non-specific down regulation of the generation of immune responses. The studies reported here extend this finding to show that such regulatory cells are also generated during the course of a mixed lymphocyte response and following stimulation of the CD3 component of the T cell receptor with anti-CD3 antibody. Regulatory activity was assessed by the addition of putative regulatory cells to mixed lymphocyte cultures and measuring the generation of cytolytic T cell activity in these cultures. The action of such non-specific regulatory cells was blocked by antiserum to the N-terminal sequence of soluble immune response suppressor (SIRS). In addition, generation of the regulatory cell population was inhibited by antiserum to the 55 kd subunit of the IL-2 receptor. A survey of cytokines showed that while IL-2 was able to stimulate the generation of non-specific regulatory cell activity, IL-1,3,6,7, and 10 as well as TNF alpha and TGF beta were unable to generate such activity. IL-2 was unable to generate non-specific suppressive activity in the presence of anti-IL-2 receptor antibody. The regulatory activity of cells generated by IL-2 was inhibited by the anti-SIRS antiserum.","PeriodicalId":18130,"journal":{"name":"Lymphokine research","volume":"9 4","pages":"499-506"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13246269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A possible role for protein kinase C activity but not cyclic nucleotide-dependent protein kinases in human natural killer cell lytic activity. 蛋白激酶C活性而非环核苷酸依赖性蛋白激酶在人自然杀伤细胞裂解活性中的可能作用。

Lymphokine research Pub Date : 1990-01-01

C C Hager, K C Petroni, M A Boyce, L D Forester, T N Oeltmann

引用次数: 0

Cholera and pertussis exotoxins protect mice against the lethal Schwartzman reaction and antagonize the effects of lipopolysaccharide on second messenger systems. 霍乱和百日咳外毒素保护小鼠免受致命的施瓦茨曼反应，并拮抗脂多糖对第二信使系统的影响。

Lymphokine research Pub Date : 1990-01-01

Y Cabeza-Arvelaiz, C Dillen, H Heremans, J Van Damme, J Cailleau, A Billiau, G Opdenakker

{"title":"Cholera and pertussis exotoxins protect mice against the lethal Schwartzman reaction and antagonize the effects of lipopolysaccharide on second messenger systems.","authors":"Y Cabeza-Arvelaiz, C Dillen, H Heremans, J Van Damme, J Cailleau, A Billiau, G Opdenakker","doi":"","DOIUrl":"","url":null,"abstract":"Pertussis toxin, and also cholera toxin are capable of inhibiting the effects of LPS in the elicitation of the generalized Schwartzman reaction. This is a potentially lethal generalized thrombo-haemorrhagic hypersensitivity and inflammatory-type response that occurs after two consecutive injections of LPS. The two exotoxins furnish significant protection against the lethal outcome of this reaction. It is known that the acute haematological and haemodynamic changes are accompanied by alterations in the levels of various endogenous mediators: glucocorticoid hormones, prostaglandins, arachidonic acid metabolites, cytokines and proteases. In vitro effects of LPS on murine leukocyte cell lines can be antagonized by pertussis toxin, implicating a Gi-like regulatory protein in the mediation of these effects. Experiments designed to study the involvement of particular second messenger systems (cAMP and phosphatidylinositol) used by LPS in vivo, revealed that the protective effects conferred by these exotoxins are associated with the antagonization of alterations caused by LPS. No correlation was found between the levels of IL-6 and the mortality rate in this experimental mouse model. The results indicate that G proteins play a role in the generation of the Schwartzman reaction and open a new approach for pharmacological intervention in endotoxemia and in clinical settings with Gram-negative sepsis.","PeriodicalId":18130,"journal":{"name":"Lymphokine research","volume":"9 2","pages":"125-35"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13313398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between the circulating concentrations of interleukin 6 (IL-6), tumor necrosis factor (TNF) and the acute phase response to elective surgery and accidental injury. 循环白细胞介素6 (IL-6)、肿瘤坏死因子(TNF)浓度与择期手术和意外损伤急性期反应的关系

Lymphokine research Pub Date : 1990-01-01

E A Pullicino, F Carli, S Poole, B Rafferty, S T Malik, M Elia

{"title":"The relationship between the circulating concentrations of interleukin 6 (IL-6), tumor necrosis factor (TNF) and the acute phase response to elective surgery and accidental injury.","authors":"E A Pullicino, F Carli, S Poole, B Rafferty, S T Malik, M Elia","doi":"","DOIUrl":"","url":null,"abstract":"The possible role of Interleukin 6 (IL-6) and tumour necrosis factor (TNF) in the regulation of the acute phase response to injury was studied in thirteen subjects undergoing elective surgery or suffering from multiple accidental injuries. The temporal changes in the circulating concentrations of these cytokines were related to the circulating acute phase protein concentrations (C-reactive protein and alpha 1 antichymotrypsin), white cell count, blood loss and duration of surgery. Circulating acute phase protein concentrations rose in all subjects during a thirty hour period following injury but none of the subjects showed a detectable rise in circulating concentrations of TNF. Peak circulating concentrations of IL-6 were detected between 4-6 hours after surgery/trauma but these showed a poor correlation with blood loss, fever, white cell count or duration of surgery. The peak concentrations of IL-6 typically occurred before the rise in circulating acute phase protein concentration. The data do not suggest a role for circulating TNF in the regulation of the acute phase response to injury. In contrast, IL-6 appears to be variably involved in the acute phase response but its precise role in mediating fever, leucocytosis and synthesis of acute phase proteins is as yet uncertain.","PeriodicalId":18130,"journal":{"name":"Lymphokine research","volume":"9 2","pages":"231-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12857096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adjuvanticity of recombinant bovine interleukin-1 beta: influence on immunity, infection, and latency in a bovine herpesvirus-1 infection. 重组牛白细胞介素-1 β的佐剂性:对牛疱疹病毒-1感染的免疫、感染和潜伏期的影响

Lymphokine research Pub Date : 1990-01-01

D N Reddy, P G Reddy, H C Minocha, B W Fenwick, P E Baker, W C Davis, F Blecha

引用次数: 0