Lymphokine research最新文献_第4页

Synthetic macrophages: liposomes bearing antigen, class II MHC and membrane-IL-1. 合成巨噬细胞:含抗原脂质体，II类MHC和膜- il -1。

Lymphokine research Pub Date : 1990-01-01

O Bakouche, L B Lachman

{"title":"Synthetic macrophages: liposomes bearing antigen, class II MHC and membrane-IL-1.","authors":"O Bakouche, L B Lachman","doi":"","DOIUrl":"","url":null,"abstract":"Liposomes containing membrane-IL-1, Iak, and the antigen conalbumin were evaluated as \"synthetic macrophages.\" The role of these three molecules in macrophage-T cell interaction was studied by testing the ability of such synthetic macrophages to induce the proliferation of a T cell clone specific to conalbumin (the D10 cell line) or immune spleen cells sensitized three times in vivo with conalbumin. In the latter case, splenic macrophages were eliminated by adherence and a lysomotropic agent. The antigen conalbumin was presented on the surface of the liposomes in two forms: as native undigested protein or as a complex of Iak and naturally processed peptides isolated from peritoneal macrophages incubated with conalbumin. When the liposomes presented the Ia antigen associated with conalbumin-peptides and membrane-IL-1, the proliferation of both the D10 and the immune spleen cells was high and maximal. The proliferation response of both cell types was completely dependent on the presence of membrane-IL-1, since no proliferation occurred when liposomes were prepared with only conalbumin-peptides bound to Iak. Therefore, it could be concluded that processed antigen associated with class II MHC antigen and membrane-IL-1 were required for T cell activation. When the liposomes presented native conalbumin, Iak, and membrane-IL-1, significant proliferation occurred, but if the liposomes lacked membrane-IL-1, the proliferation of the T cell clone and the spleen cells decreased to only 40-50% of the previous signal. In this case native conalbumin and class II antigen alone were required for T cell activation, while membrane-IL-1 alone amplified the response. When the liposomes were made with only Iak and membrane-IL-1, lacking either form of conalbumin, there was no proliferation of antigen-specific target cells. These results indicated that in this synthetic system, membrane-IL-1 is essential for the proliferative response of antigen-specific T cells when conalbumin is presented as processed peptides in association with Iak.","PeriodicalId":18130,"journal":{"name":"Lymphokine research","volume":"9 3","pages":"259-81"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13547181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic behavior and distribution of the synthetic nonapeptide fragment 163-171 of human IL-1 beta. 人IL-1 β合成非肽片段163-171的代谢行为及分布

Lymphokine research Pub Date : 1990-01-01

G P Pessina, V Bocci, C Nicoletti, C Becherucci, R Presentini, L Parente, L Villa, A Tagliabue, D Boraschi

{"title":"Metabolic behavior and distribution of the synthetic nonapeptide fragment 163-171 of human IL-1 beta.","authors":"G P Pessina, V Bocci, C Nicoletti, C Becherucci, R Presentini, L Parente, L Villa, A Tagliabue, D Boraschi","doi":"","DOIUrl":"","url":null,"abstract":"The pharmacokinetic parameters and distribution of the adjuvant synthetic nonapeptide VQGEESNDK, corresponding to the fragment in position 163-171 in human IL-1, were analyzed after administration to rabbit through different routes. The radiolabeled peptide did not bind to plasma proteins and, when inoculated i.v., it disappeared very rapidly from the circulation, with a t1/2 alpha of 1 min and a t 1/2 beta of 166 min. Upon administration through i.m., s.c. and oral route, the Cmax was reached between 30 and 90 min after inoculum and ranged between 7 and 4% of the administered dose. Organ distribution showed that most of the radioactivity was concentrated in kidneys and excreted in urine. From Sephadex G-10 chromatography, about 60% of the peptide recovered in the urine 4h after i.v. inoculum was intact, whereas it was more than 85% degraded when administered by other routes. The amount of intact peptide recovered in the urine correlated with the biological effectiveness through different routes, suggesting that the adjuvant effect in vivo is exerted by the intact peptide, rather than by its metabolites.","PeriodicalId":18130,"journal":{"name":"Lymphokine research","volume":"9 3","pages":"371-9"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13547182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alpha-mannosidase treatment of NK cells does not inhibit NK cell lytic function. α -甘露糖苷酶处理NK细胞不抑制NK细胞的裂解功能。

Lymphokine research Pub Date : 1990-01-01

W H Chambers, T N Oeltmann

{"title":"Alpha-mannosidase treatment of NK cells does not inhibit NK cell lytic function.","authors":"W H Chambers, T N Oeltmann","doi":"","DOIUrl":"","url":null,"abstract":"Alpha-mannosidase was tested for its ability to inhibit lytic activity of nonadherent, mononuclear peripheral blood leukocytes (PBL) against K-562 target cells. Pretreatment of effector cells (60 min., 37 degrees C, pH 7.3) with this enzyme, prior to and after exhaustive dialysis, was examined. Nondialyzed enzyme preparations completely inhibited NK lytic function at all concentrations tested (1.0, 0.5, and 0.25 units/ml). On the other hand, dialyzed enzyme preparations had no inhibitory effect on NK lytic function over the same range of concentrations. The inhibitory effects of the nondialyzed enzyme were due to the presence of (NH4)2SO4, which could be removed by dialysis. Studies were also performed to determine whether enzyme treatment of effector cells resulted in hexose release from cell surface structures. Treatment of effector cells with alpha-mannosidase (dialyzed preparation) resulted in a dose dependent release of mannose. These data demonstrate that NK cell lytic function is not inhibited by pretreatment of effector cells with alpha-mannosidase even though mannose is quantitatively released from cell surface oligosaccharide structures. These results suggest that NK lytic function does not involve an effector cell surface structure bearing terminal alpha-linked mannose residues as previously reported.","PeriodicalId":18130,"journal":{"name":"Lymphokine research","volume":"9 2","pages":"239-45"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13488585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytokine regulation of HIV expression. 细胞因子调控HIV表达。

Lymphokine research Pub Date : 1990-01-01

A S Fauci

引用次数: 0

Induction of IL-1 by lipopolysaccharide (LPS) and its modulation by synthetic lipid A precursor Ia. 脂多糖(LPS)对IL-1的诱导及合成脂质A前体Ia对其的调节。

Lymphokine research Pub Date : 1990-01-01

H D Flad

引用次数: 0

Interferon production in sickle cell disease. 镰状细胞病中干扰素的产生

Lymphokine research Pub Date : 1990-01-01

S C Taylor, S J Shacks, S M Villicana, J Olivares, G A Dinkins

{"title":"Interferon production in sickle cell disease.","authors":"S C Taylor, S J Shacks, S M Villicana, J Olivares, G A Dinkins","doi":"","DOIUrl":"","url":null,"abstract":"There is limited data on cytokine production in sickle cell disease (SCD). In this study, both alpha (Poly IC-induced) and gamma (PHA-induced) interferon (IFN) were measured in 62 SCD steady state patients, and 21 in the crisis state associated with infections. Comparable normal controls (30 healthy and 14 with infections) were assessed in a similar manner. Gamma IFN production in both SCD groups, steady state (35 +/- 6 U/ml) and crisis state (24 +/- 11 U/ml) was significantly diminished when compared to the normal healthy controls (65 +/- 14 U/ml) with P less than .005. Both SCD groups were also less than normals with infections (56 +/- 23 U/ml) with P less than .005. On closer analysis of individual titers, 15/62 (24%) in the steady state, 11/21 (52%) of SCD patients with infection and 2/14 (14%) of normals with infection, showed impaired gamma IFN when compared to normals without infection (range 27-2187 U/ml). Alpha IFN production in the SCD groups; steady state (512 +/- 113 U/ml) and SCD crisis with infection (559 +/- 110 U/ml) was virtually equivalent to normals (524 +/- 170 U/ml) and normals with infection (509 +/- 116 U/ml). Analysis of individual titers, in contrast to gamma IFN, also showed no significant differences. These results indicate that a significant percentage of SCD patients in both the steady state and infectious state associated with crisis, have impaired gamma IFN production. In view of the known immunomodulatory functions of gamma IFN, this apparent defect may be another factor to explain the increased frequency and severity of infections in SCD.","PeriodicalId":18130,"journal":{"name":"Lymphokine research","volume":"9 3","pages":"415-23"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12862168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumor-induced tumor necrosis factor production in macrophages. 巨噬细胞中肿瘤诱导的肿瘤坏死因子的产生。

Lymphokine research Pub Date : 1990-01-01

D N Männel, R Jänicke, U Westenfelder, B Echtenacher, A Kist, W Falk

引用次数: 0

Functional expression of native and chimeric human and murine IL-2 receptor p70 chains in an IL-3-dependent murine cell line. 人-鼠IL-2受体p70链在il -3依赖性小鼠细胞系中的功能表达

Lymphokine research Pub Date : 1990-01-01

B D Freimark, R J Robb

{"title":"Functional expression of native and chimeric human and murine IL-2 receptor p70 chains in an IL-3-dependent murine cell line.","authors":"B D Freimark, R J Robb","doi":"","DOIUrl":"","url":null,"abstract":"Full length cDNAs encoding the human and murine p70 genes were isolated using polymerase chain reaction (PCR) and conventional cDNA library screening techniques, respectively. To validate their functional potential, expression vectors containing human, murine and chimeric human/murine p70 cDNAs were transfected into the murine IL-3-dependent cell line FDC-P1. Transfected cells expressed a combination of high and low-affinity IL-2 binding sites while parental cells displayed only the low-affinity sites associated with expression of endogenous p55 IL-2 receptor chains. The role of the transfected p70 chains in formation of the high-affinity receptor sites was confirmed by the finding that the species-specific inhibitory antibody TU27 blocked high-affinity binding to human p70 and chimeric human/murine p70-expressing cells while having no effect on cells transfected with the murine p70 cDNA construct. As consequences of the expression of the transfected p70 chains, the cells responded to IL-2 with increased levels of endogenous p55 receptor subunit and both short and long-term proliferation. These studies substantiate the role of the p70 receptor chain in functional responses to IL-2 and provide a model system for future dissection of the structure/function relationships of the IL-2 receptor.","PeriodicalId":18130,"journal":{"name":"Lymphokine research","volume":"9 4","pages":"507-15"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13246270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production, characterization and use of five monoclonal antibodies to human IL-4. 五种人IL-4单克隆抗体的制备、鉴定和应用。

Lymphokine research Pub Date : 1990-01-01

M G Scott, A D Levine, A W Butch, M B Bowen, K A Macke, M H Nahm

引用次数: 0

Responses of pokeweed mitogen-stimulated peripheral mononuclear cells to human recombinant interleukins 3 and 4. 商陆有丝分裂原刺激的外周单核细胞对人重组白细胞介素3和4的反应。

Lymphokine research Pub Date : 1990-01-01

M Kato, M Fujii, T Ito, K Kumagai

{"title":"Responses of pokeweed mitogen-stimulated peripheral mononuclear cells to human recombinant interleukins 3 and 4.","authors":"M Kato, M Fujii, T Ito, K Kumagai","doi":"","DOIUrl":"","url":null,"abstract":"Peripheral blood mononuclear cells (PBMC) that had been stimulated with a polyclonal B cell activator, pokeweed mitogen (PWM), were examined for mitogenic responsiveness to interleukins (IL) and colony stimulating factors (CSF) or other cytokines using recombinant preparations from E. coli or Cos cells expressing their cloned cDNAs. PWM-stimulated PBMC (PWM-blasts) were found to be responsive to IL-3 depending upon the concentrations of the agent. Mitogenic response of PWM-blasts in the reaction to IL-3 was suppressed by the addition of a specific monoclonal antibody against IL-3 but not by anti-IL-2 serum, excluding the possibility that the DNA replication induced by IL-3 is a result of the proliferative response of IL-2 responder cells to IL-2, which might be produced during the culture of PWM-blasts in the presence of IL-3. The PWM-blasts were also responsive to IL-2 and IL-4 but not to other interleukins, namely, IL-1, IL-5 and IL-6, nor to granulocyte/macrophage (GM)-, granulocyte (G)- nor macrophage (M)-CSFs. They were also not responsive to interferon gamma. Similar responsiveness to IL-3 and IL-4 was found in the PBMC stimulated with a B-cell mitogen, Staphylococcus aureus Cowan 1 (Sac-1), but not in the PBMC stimulated with T cell mitogens, PHA or ConA. These results suggest that the human PBMC stimulated with B cell mitogens such as PWM or Sac-1 contain activated IL-3- and IL-4- responsive cells in sufficient number to detect these lymphokine activities.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":18130,"journal":{"name":"Lymphokine research","volume":"9 2","pages":"247-55"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12857097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0