Adjuvanticity of recombinant bovine interleukin-1 beta: influence on immunity, infection, and latency in a bovine herpesvirus-1 infection.

Lymphokine research Pub Date : 1990-01-01
D N Reddy, P G Reddy, H C Minocha, B W Fenwick, P E Baker, W C Davis, F Blecha
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Abstract

Recombinant bovine interleukin-1 beta (rBoIL-1 beta) was administered to calves in conjunction with a bovine herpesvirus-1 (BHV-1) vaccine. All calves were immunized against BHV-1 and three groups received rBoIL-1 beta at 33, 100, or 330 ng/kg on days 1 and 15; control animals received physiological saline. All calves were challenged with BHV-1 on day 22. Total leukocytes were increased by rBoIL-1 beta, primarily by causing neutrophilia and monocytosis; CD4/CD8 ratios tended to be increased in rBoIL-1 beta-treated animals. Serum neutralizing antibody titers and cytotoxic responses against BHV-1-infected bovine kidney fibroblasts were increased and virus excretion was decreased in rBoIL-1 beta-treated calves. On days 58 and 59, control and 100 ng/kg rBoIL-1 beta-treated calves were injected with dexamethasone (.04 mg/kg). Virus excretion was less and clinical signs of BHV-1 infection were lower in rBoIL-1 beta-treated calves after dexamethasone injection. These data suggest that rBoIL-1 beta may be an effective adjuvant to BHV-1 immunization.

重组牛白细胞介素-1 β的佐剂性:对牛疱疹病毒-1感染的免疫、感染和潜伏期的影响
重组牛白细胞介素-1 β (rBoIL-1 β)与牛疱疹病毒-1 (BHV-1)疫苗一起施用于犊牛。所有犊牛均接种BHV-1疫苗,3组犊牛分别在第1天和第15天接种剂量为33、100和330 ng/kg的rBoIL-1 β;对照动物接受生理盐水。所有犊牛在第22天注射BHV-1。rBoIL-1 β增加了白细胞总数,主要是通过引起嗜中性粒细胞增多和单核细胞增多;在rBoIL-1 β处理的动物中,CD4/CD8比值趋于升高。rBoIL-1 β处理小牛的血清中和抗体滴度和抗bhv -1感染的牛肾成纤维细胞的细胞毒反应增加,病毒排泄减少。在第58天和第59天,对照和100 ng/kg rBoIL-1 β处理的犊牛注射地塞米松。04毫克/公斤)。注射地塞米松后,经rBoIL-1 β处理的小牛体内的病毒排泄量较少,BHV-1感染的临床症状也较低。这些数据表明rBoIL-1 β可能是BHV-1免疫的有效佐剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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