{"title":"STUDIES ON MICROBIAL DIVERSITY OF POLYGALACTURONASE PRODUCING BACTERIA FROM THE SOIL OF VEGETABLES MARKET OF PATAN","authors":"Sanjay K. S. Patel, D. Pandya, S. Bhatt","doi":"10.1234/LSL.V59I0.208","DOIUrl":"https://doi.org/10.1234/LSL.V59I0.208","url":null,"abstract":"ABSTRACT: Present work is carried out to check the study microbial diversity of polygalacturonase producing bacteria from the soil of fruits and vegetables market. Soil which is continuously dumped with waste of fruits and vegetables are rich sources of biopolymer viz, pectin, cellulose and hemicellulose. Presence of such biopolymer selectively promotes the growth of microorganisms which have potential to degrade pectin. In this study more than one hundred and twenty bacterial species are isolated from the soil on the basis of their cell and colony morphology. From these isolates, forty five bacterial species found to produce polygalacturonase enzyme and same have check for capability to produce protease and amylase enzyme. This study provides useful information of the microbial diversity of the soil of fruits and vegetable market. Further it is helpful to study the metabolism of microorganisms to degrade diverse biopolymer.","PeriodicalId":18074,"journal":{"name":"LIFE SCIENCES LEAFLETS","volume":"201 1","pages":"16-21"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83465137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"SODIUM FLUORIDE ALTERATION OF PROTEIN CONTENT VIS-À-VIS ELECTROPHORETIC PATTERN OF MUSCLE ESTERASES (E.C.3.1.1.1) IN POECILIA RETICULATA PETERS ON CHRONIC EXPOSURE","authors":"Hitesh U. Shingadia and E.R. Agharia","doi":"10.1234/LSL.V59I0.206","DOIUrl":"https://doi.org/10.1234/LSL.V59I0.206","url":null,"abstract":"ABSTRACT: A progressive reduction in protein content observed in the muscle of fish in present study was both as a function of time as well as increase in the concentration of fluoride. During chronic exposure to sodium fluoride, the banding pattern of esterase diminished in the treated group of fish viz. lowest (5.75 ppm), lower intermediate (7.18 ppm), higher intermediate (9.58 ppm) and highest (14.37 ppm) concentration of the 24 hrs. LC 50 (115 ppm) value when compared with the control group. SDS-PAGE and staining of the gel revealed that esterase in muscle of fish from control group resolved into six bands (lane-1). Exposure of fluoride to all the four concentrations showed significantly faint and diffused banding pattern of esterases and complete loss of esterase band-1 (Lane 2-5), probably due to chronic stress induced by fluoride. The esterase from band-1 might be sensitive to fluoride intoxication, thus completely vanished during chronic treatment. However in the higher intermediate (9.58 ppm) and highest (14.37 ppm) sodium fluoride treatment groups, sixth band of esterase (lane 4-5) was found to be very faintly visible on staining. Decrement in protein content & diminution of certain esterase bands in the muscle tissue of the treated group suggest soft tissue (non-skeletal) fluorosis induced by sodium fluoride during chronic exposure period, probably could be due to inhibition of biosynthetic mechanism of proteins vis-a-vis esterases. The study of esterase in fish calls attention to sensitive indicator of the environmental pollutants and can be used as contrivance in study of environmental dilapidations. KEY WORDS: Protein, Esterase isozymes, Electrophoresis, Sodium fluoride, Poecilia reticulata.","PeriodicalId":18074,"journal":{"name":"LIFE SCIENCES LEAFLETS","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78228554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"SEED GERMINATION OF SANDAL (SANTALUM ALBUM LINN.): INFLUENCE OF LIGHT QUALITY AND SEED SCARIFICATION","authors":"N. VishalVijayan–Rahees","doi":"10.1234/LSL.V59I0.214","DOIUrl":"https://doi.org/10.1234/LSL.V59I0.214","url":null,"abstract":"ABSTRACT: Santalum album is a hemiparasitic tree disseminated rather widely in India. The influence of light quality on germination of sandal ( Santalum album Linn. ) was investigated in College of Forestry, Kerala Agricultural University during March – July 2014. The literature on how the light environment influences seed germination in Santalum album is contradictory. In our trials, seeds treated with GA, water and fire covered with black, blue red and clear plastic papers and at full light condition where carried to find out impact of distinctive light qualities. Highest germination percentages were acquired under full light condition (50.3%) when treated with GA followed by GA treated seeds covered with clear plastic paper or transparent light (49.3%). Red and blue light treatment indicates most minimal germination rate, virtually few germination occurred in the dark ambience.","PeriodicalId":18074,"journal":{"name":"LIFE SCIENCES LEAFLETS","volume":"44 1","pages":"90-92"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80915644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"INTERACTION OF COLLETOTRICHUM CAPSICI IN CHILLI VARIETY","authors":"S. Kumar, Vinay Kumar, Vineeta Singh","doi":"10.1234/LSL.V60I0.233","DOIUrl":"https://doi.org/10.1234/LSL.V60I0.233","url":null,"abstract":"ABSTRACT: Twelve isolates of Colletotrichum capsici from various places were characterized into three groups based on morphological characters and growth rate. Different isolates (Cc1,Cc4,Cc6, Cc7, Cc8, Cc10 and Cc12) are present in middle virulence (II group), isolates (Cc3,Cc5 and Cc9) are present in low virulence (III group) and isolate Cc2,Cc11 is present in High virulence (I group). The effect of Colletotrichum capsiciwas tested onfive chilli varieties for their disease interactions. The results revealed that these isolates could be differentiated in disease severity. The degree of disease severity as a size of necrotic symptom was different on each tested variety and varied from 0.47 to 2.68.The mean value of 12 isolates is varied from 1.67 to 4.01mm/lesion diameter while mean value of five variety is varied from 0.47 to 2.71 mm/lesion diameter and CD of five variety is 0.0 – 2.21 mm/lesion diameter. These varieties show 7.38 mm critical variance in five varieties.In twelve isolates the lesion diameter is varied from 3.09 to 21.01. KEY WORDS: Chilli, Anthracnose, Colletotrichum Capsici, Disease Severity, CD.","PeriodicalId":18074,"journal":{"name":"LIFE SCIENCES LEAFLETS","volume":"46 1","pages":"41-48"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85982327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Palanikumaran, K. Parthiban, I. Sekar, R. Umarani, D. Amirtham
{"title":"Variability studies for seed and seedling traits in Pongamia pinnata (L.) Pierre at Tamilnadu.","authors":"B. Palanikumaran, K. Parthiban, I. Sekar, R. Umarani, D. Amirtham","doi":"10.5958/0975-928X.2016.00084.3","DOIUrl":"https://doi.org/10.5958/0975-928X.2016.00084.3","url":null,"abstract":"The present investigation was carried out at Forest College and Research Institute, Mettupalayam, Tamil Nadu to identify the best half sibs of Pongamia pinnata across its natural distribution areas in south India for further collection of seeds for afforestation or breeding purpose. The seeds were collected from different agro climatic zones of Tamil Nadu. Seeds were measured for its length, width, thickness, test weight and volume and then sown in nursery to study the variation in germination and initial growth parameters and biomass viz., germination per cent, germination vigour, shoot length, root length and root and shoot dry weight of seedlings. Seeds collected from southern zone were superior compared to seeds from other parts in all the traits considered for the study. These seed sources can be further screened for tree improvement traits considering their immense value in yielding bio diesel.","PeriodicalId":18074,"journal":{"name":"LIFE SCIENCES LEAFLETS","volume":"10 1","pages":"160-168"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72878695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"THE DOCTRINE OF SIGNATURES: A WARFARE BETWEEN MYTH AND REALITY","authors":"D. Patil","doi":"10.1234/LSL.V59I0.210","DOIUrl":"https://doi.org/10.1234/LSL.V59I0.210","url":null,"abstract":"ABSTRACT:The plants and animals, from the very beginning of their existence, are being studied by two widely distinct standpoints viz., (i) the philosophical and (ii) the utilitarian. The plants especially from the latter standpoint were/are studied as a source of medicine and agriculture. The science of medicine was/is based on ‘cause and effects’, apart from certain other theories and philosophies. One such philosophy is ‘the doctrine of signatures’. However, it has been criticized since its beginning. The present communication is the result of analysis of the knowledge of ancients and author’s own observations. It is aimed at inviting attention of intellectuals to revive and point out relevance in its totality.The paper sheds light on history and development of this doctrine, contributions of pioneers, types of signature, background of planetary influence, comparison of signatures from ethnomedicine to modern medicine, echo of doctrine of signatures on common plant names in various human societies, various views of proponents and opponents of the doctrine, etc. It is concluded that the drug sources based on the doctrine alongwith verification on scientific lines is a better way to human welfare.KEY WORDS: Doctrine of signatures, History, Myth, Reality.","PeriodicalId":18074,"journal":{"name":"LIFE SCIENCES LEAFLETS","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83213212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ANTIOXIDANT ACTIVITY IN CALLUS CULTURES AND IN VITRO REGENERATED PLANTS OF ARTEMISIA NILAGIRICA (CLARKE) PAMP.- AN IMPORTANT MEDICINAL PLANT SPECIES","authors":"A. Baliarsingh, L. Samanta, S. Mohanty","doi":"10.1234/LSL.V60I0.230","DOIUrl":"https://doi.org/10.1234/LSL.V60I0.230","url":null,"abstract":"ABSTRACT: Antioxidant potential of in vitro callus and regenerated plants of Artemisia nilagirica was investigated using several biochemical assay techniques for scavenging of 1,1-diphenyl -2-picryl hydrazyl (DPPH), nitric oxide, superoxide and hydroxyl radicals as well as lipid peroxidation. The internodal explants from A. nilagirica were cultured on Murashige and Skoog’s (1962) basal medium (MS) supplemented with various concentrations and combinations of plant growth regulators. A synergistic coupling of 0.5 mg/L 2,4- dichlorophenoxyacetic acid (2,4-D) with 1.0 mg/L Kinetin (Kin) yielded maximum callogenic response. Shoot organogenesis in callus cultures was most favoured in MS containing 2.0 mg/L 6-benzylaminopurine (BAP) and 0.5 mg/L indole-3-acetic acid (IAA). In vitro regenerated plantlets, emerged from culture medium, were acclimatized and the survival rate of ex vitro plants after soil transplantation was 80-83% with no apparent phenotypic variations. The antioxidant potential of natural (in vivo) plants, callus tissues and in vitro regenerated plants before and after field transplantation (ex vitro) plants were compared. DPPH scavenging activity was the highest in aqueous extracts of 10 week-old ex vitro plants than other sources. Superoxide anion and nitric oxide radical scavenging activity was the highest in ethanolic extracts of 10 week-old ex vitro plants where as the hydroxyl radical was the maximum in 6 week-old in vivo plants. Lipid peroxidation was neither observed in calli nor in regenerated plants of A. nilagirica.","PeriodicalId":18074,"journal":{"name":"LIFE SCIENCES LEAFLETS","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85267279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Degradation of textile dyes by white rot basidiomycetes.","authors":"B. Parmar, P. Mervana, B. Vyas","doi":"10.1234/LSL.V59I0.212","DOIUrl":"https://doi.org/10.1234/LSL.V59I0.212","url":null,"abstract":"ABSTRACT: Dyes released by the textile industries pose a threat to environmental quality. Ligninolytic white-rot basidiomycetes can effectively degrade colored effluents and conventional dyes. White-rot fungi produce various isoforms of extracellular oxidases including laccase, Mn peroxidase and lignin peroxidase (LiP), which are involved in the degradation of lignin in their natural lignocellulosic substrates. The textile industry, by far the most avid user of synthetic dyes, is in need of eco-efficient solutions for its colored effluents. White rot basidiomycetous fungi comprise the only group of organisms known to completely degrade lignin. Ligninolytic enzymes have potential applications in a large number of fields, including the chemical, fuel, food, agricultural, paper, textile, cosmetic industrial sectors and more. This ligninolytic system of white-rot fungi is also directly involved in the degradation of various xenobiotic compounds apart from textile dyes. Their capacities to remove xenobiotic substances make them a useful tool for bioremediation purposes. This paper reviews involvement of ligninolytic enzymes of white rot basidiomycetes in the degradation of textiles dyes and xenobiotic compounds for their industrial and biotechnological applications.","PeriodicalId":18074,"journal":{"name":"LIFE SCIENCES LEAFLETS","volume":"30 1","pages":"62-75"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84184206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Chavan, Kerthika Armughum, A. Jha, A. Handa, R. Newaj, S. Dhyani
{"title":"Growth performance of 12 year old air layered Madhuca latifolia","authors":"S. Chavan, Kerthika Armughum, A. Jha, A. Handa, R. Newaj, S. Dhyani","doi":"10.1234/LSL.V60I0.184","DOIUrl":"https://doi.org/10.1234/LSL.V60I0.184","url":null,"abstract":"Madhuca latifolia commonly known as mahua belongs to the family Sapotaceae is an important economic tree growing throughout India and most preferable in day to day activity of tribal people. It is distributed in warm and humid regions of Central and south India such as eastern Uttar Pradesh, Chhattisgarh, Maharashtra, Bihar, Jharkhand, Orissa and Andhra Prades. It is concluded that, the growth of air-layered Mahua is better when compared to published literature on seedling originated mahus. Based on this study, it is proved that air layering of mahua is cheap and best method to produce quality planting material. Further large scale investigation on performance of air-layered mahua plantations will provide early returns in terms of flowers and seed to sustain local livelihood. The stature of air layered mahua is well suited for agroforestry plantations.","PeriodicalId":18074,"journal":{"name":"LIFE SCIENCES LEAFLETS","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87024212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"NON TIMBER FOREST PRODUCTS UTILIZATION DURING ADVERSE CLIMATIC CONDITIONS : A CASE OF SAORA TRIBES OF GANJAM DISTRICT, ODISHA","authors":"M. Mishra, P. A. Jadhav, M. Shrivastava","doi":"10.1234/LSL.V54I0.134","DOIUrl":"https://doi.org/10.1234/LSL.V54I0.134","url":null,"abstract":"Saoras are one of the most ancient tribes in Odisha mentioned in Hindu myths and classics are also expert climbers and hunters. Saoras of Ganjam district mostly depend on forest for their livelihood and the area is rich in biodiversity and also have rare medicinal flora. They eat several NTFPs, wild roots, rhizome, seeds, fruits and mushrooms during harsh climatic conditions like severe drought. They use stored wild tubers mostly Dioscoreas like Bowla ( Dioscorea bulbifera L.), Bonda, Chun aru ( D. daemona Roxb.), Karondi aru ( D.bellophylla L.), Pani aru ( D. oppositifolia L.) and Arguna ( Cycas spp. ) to fulfill their food, medicinal and nutritional requirements particularly in harsh climatic conditions. They eat various wild leafy vegetables like Kolod ( Lathyrus sativa L.),Bilo ( Pisum sativum L.), Kulthi ( Microtylum uniflorum Lam.), Banana stem ( Musa superb a L.) Ban poi ( Basella alba L.). They also utilize NTFPs like Bamboo karda ( Bambusa vulgaris Schrad exJC Wendl.), Moha ( Madhuca indica J.F Gmel.), Amla ( Emblica officinalis Gaertn.), fruits. Some wild mushrooms like Banschattu, Ambachattu ( Agaricus spp. ) after drying and crushing were eaten by them. Indigenous practices of Saora tribes to cope up extreme environmental conditions and utilization of forest products during drought were discussed in the paper.","PeriodicalId":18074,"journal":{"name":"LIFE SCIENCES LEAFLETS","volume":"119 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75803708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}