International Journal of Molecular Biology Open Access最新文献

The comparison mechanisms of cancer immunotherapy in new method cancer treatment with some modern methods of cancer therapies 癌症治疗新方法中的癌症免疫疗法与一些现代癌症治疗方法的机制比较

International Journal of Molecular Biology Open Access Pub Date : 2024-07-09 DOI: 10.15406/ijmboa.2024.07.00175

Ponizovskiy Mr

{"title":"The comparison mechanisms of cancer immunotherapy in new method cancer treatment with some modern methods of cancer therapies","authors":"Ponizovskiy Mr","doi":"10.15406/ijmboa.2024.07.00175","DOIUrl":"https://doi.org/10.15406/ijmboa.2024.07.00175","url":null,"abstract":"There were compared the roles of the different methods of Cancer treatments, especially concentrating on advantage of new method of cancer treatment over some negative results appearances of modern methods cancer chemotherapy with large dosage cytotoxic drugs. Thus the current discoveries of influences on the interactions between proteins like CTLA-4, PD-1 and CD-28, CD-80 (B-7-1), CD-86 (B-7-2) on processes for regulation of T cells immune activities that induces to make comparisons to the positive conditions for Cancer immunotherapy versus negative condition for cancer immunotherapy. The immune processes in new method of cancer treatment through Prolonged medical Starvation 42 - 45 days via supporting by extracts of plants with giving very small quantity of cytotoxic substances demand the favourable conditions for concomitant Cancer immunotherapy versus negative conditions of Cancer chemotherapy being created with large dosage cytotoxic drugs. Also there were estimated comparisons of the following methods of Cancer therapies: Surgery therapy, local X-ray therapy, local therapy with radioactive ions. Besides there were shown the influences immune defensive mechanisms on regulatory processes of maintenance stability of Internal Energy in able-bodied organism causing Stationary State of an organism in norm as well as suppression of the influences immune defensive mechanisms on regulatory processes violating maintenance stability Internal Energy of an organism that causes Quasistationary pathologic State of cancer disease metabolic processes. Therefore the favourable condition of mechanism newer method of cancer treatment via “Prolonged medical Starvation (during 42-45 days)” via supporting by herbal extracts induces cancer depression, and using very small dosage weak cytotoxic substances cause destruction cancer cells forming normal condition for operation of immune T cells and macrophages for autophagy promoting best treatment of cancer diseases.","PeriodicalId":179796,"journal":{"name":"International Journal of Molecular Biology Open Access","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141835915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of MicroRNAs in regulating genes related to neurodegenerative diseases MicroRNA 在调节神经退行性疾病相关基因中的作用

International Journal of Molecular Biology Open Access Pub Date : 2024-07-01 DOI: 10.15406/ijmboa.2024.07.00174

Joao Vitor Carvalho Piasentim, Samya Nogueira Asseiss

引用次数: 0

Why are there so few studies on plant diversity chemical types? - how ecotypes and chemotypes can unlock insights into environmental and community health 为什么对植物多样性化学类型的研究如此之少？- 生态型和化学型如何揭示环境和社区健康的奥秘

International Journal of Molecular Biology Open Access Pub Date : 2024-02-27 DOI: 10.15406/ijmboa.2024.07.00162

Philippe Barreto de Almeida, Jéssica Regina Sales Felisberto, Cristiane Flora Villarreal, Renato Crespo Pereira, Davyson de Lima Moreira, Ygor Jessé Ramos

引用次数: 0

Evaluation of antibacterial and antioxidant properties of Lavandula officinalis extracts obtained by ultrasound 评估超声波提取的薰衣草提取物的抗菌和抗氧化特性

International Journal of Molecular Biology Open Access Pub Date : 2024-02-13 DOI: 10.15406/ijmboa.2024.07.00159

Wendy Yaneth Villastrigo López, Christian Javier Cabello Alvarado, M. D. Dávila Medina, A. C. Castañeda Facio, A. S. Galindo

{"title":"Evaluation of antibacterial and antioxidant properties of Lavandula officinalis extracts obtained by ultrasound","authors":"Wendy Yaneth Villastrigo López, Christian Javier Cabello Alvarado, M. D. Dávila Medina, A. C. Castañeda Facio, A. S. Galindo","doi":"10.15406/ijmboa.2024.07.00159","DOIUrl":"https://doi.org/10.15406/ijmboa.2024.07.00159","url":null,"abstract":"Lavandula officinalis is a Mediterranean plant to which antimicrobial and antioxidant properties are attributed. The objective of the research was to evaluate the chemical composition, antioxidant and antibacterial activity of dried leaves of Lavandula officinalis. For the extraction of bioactive compounds, ultrasound was used, which is an environmentally friendly technique, where a yield percentage of 15.51% was obtained. The chemical composition was evaluated by high-performance liquid chromatography coupled to mass (HPLC-MS), where it was demonstrated that it was possible to extract compounds such as rosmarinic acid, medioresinol, which are the main compounds to which the properties of this plant are attributed. The antioxidant evaluation was carried out by DPPH, ABTS, and FRAP, where inhibition percentages of 60.99 %, 79.86 %, and 0.07 mg Trolox/mL, respectively, were found. The antibacterial activity against Staphylococcus aureus and Escherichia coli was evaluated by the agar diffusion method, finding inhibition halos of 10 mm and 9 mm, respectively. This demonstrates that the bioactive compounds contained in Lavandula officinalis are an alternative to the synthetic compounds currently used in the cosmetic, food, and pharmaceutical industries due to the properties they possess.","PeriodicalId":179796,"journal":{"name":"International Journal of Molecular Biology Open Access","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140457181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterisation of pathogens isolated from egg samples 从鸡蛋样本中分离出的病原体的分子特征

International Journal of Molecular Biology Open Access Pub Date : 2024-01-10 DOI: 10.15406/ijmboa.2024.07.00157

Sagadevan P, Jenifer Christina, Raghunath M, Nagarajan M, Premalatha S, Preeethi R, Janarthanan P

{"title":"Molecular characterisation of pathogens isolated from egg samples","authors":"Sagadevan P, Jenifer Christina, Raghunath M, Nagarajan M, Premalatha S, Preeethi R, Janarthanan P","doi":"10.15406/ijmboa.2024.07.00157","DOIUrl":"https://doi.org/10.15406/ijmboa.2024.07.00157","url":null,"abstract":"ceA on the biochemical analysis of this study, which is similar to a battery of biochemical tests including methyl red, Voges-Proskauer, indole and citrate, the organisms were determined to be Klebsiella. In the present study, the highest resistance rate was observed for inoflavuxin (50%) and ceftazidimine (80%), which is similar to the highest resistance rate for cefotaxime (85%) and ceftazidime (68%), with the MIC ranging from 32 to 256 mg/l. The Pseudomonas and Klebsiella plasmid isolation in the present study was found to have a molecular weight of 2.6 kb and 2.7 kb, which is similar to most isolates harboring multiple plasmids ranging in size from about 2 kb to over 150 kb included. Seventy-nine isolates contained plasmids larger than 50 kb, and some contained multiple large plasmids. In the present study, the molecular weight of Pseudomonas and Klebsiella was found to be 356 base pairs and 359 base pairs, respectively, which is similar to that of the 85 clinical isolates of Klebsiella pneumonia screened by PCR amplification of the antiseptic resistance genes qacE1 and ceA.","PeriodicalId":179796,"journal":{"name":"International Journal of Molecular Biology Open Access","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140511142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Profiling of fungi associated with Lycopersicum esculentum mill. spoilage in Nigeria using internal transcribed spacer (its) sequence of rDNA method 与番茄有关的真菌图谱。利用rDNA内转录间隔段(its)序列法测定尼日利亚的腐败

International Journal of Molecular Biology Open Access Pub Date : 2023-08-31 DOI: 10.15406/ijmboa.2023.06.00149

Elijah Nya, Atim Peter, O. Udofia

{"title":"Profiling of fungi associated with Lycopersicum esculentum mill. spoilage in Nigeria using internal transcribed spacer (its) sequence of rDNA method","authors":"Elijah Nya, Atim Peter, O. Udofia","doi":"10.15406/ijmboa.2023.06.00149","DOIUrl":"https://doi.org/10.15406/ijmboa.2023.06.00149","url":null,"abstract":"Lycopersicum esculentum Mill often referred to as Tomato is among important vegetable fruits widely cultivated the world over. The fruits are made up of soft endocarp which make them susceptible to spoilage by fungi mold. The fungi produce mycotoxins that are injurious and detrimental to human health. This study is aimed at profiling fungi mold associated with Lycopersicum esculentum rot /spoilage and identify the putative fungi responsible for its spoilage in Nigeria, particularly Akwa Ibom State metropolis. Tomato fruits heavily infested with fungi mold were collected and marshed using a sterile electric blender. Serial dilution of sample in normal saline were made and grown on prepared plates of Potato Dextrose Agar (PDA) to obtain pure culture. High quality genomic DNA was isolated from the samples; it was quantified and checked for purity. The DNA samples were subjected to PCR amplification. The amplicons were gel eluted, sequenced and checked for homology by using Basic local alignment search tool - BLAST. Identification was obtained from the top similarility outcomes of the samples. Four fungal isolates identified were Aspergilus spp., Fusarium spp., Mucor spp. and Penicillium citrinium. The economic importance of fungi molds are not in doubt as some of them are important precursor of diseases as they produces mycotoxins which is capable of causing diseases in both humans and animals, while other sare important in the field of medicine, in industrial production of antibiotics especially Penicillin.","PeriodicalId":179796,"journal":{"name":"International Journal of Molecular Biology Open Access","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123384241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulatory long noncoding RNAs in cardiovascular development and congenital heart defects 调控长链非编码rna在心血管发育和先天性心脏缺陷中的作用

International Journal of Molecular Biology Open Access Pub Date : 2023-06-14 DOI: 10.15406/ijmboa.2023.06.00147

Marlin Touma

引用次数: 0

Genetic characterization of patas monkey (Erythrocebus patas) in new-bussa, niger state, Nigeria 尼日利亚尼日尔州新布萨地区赤猴(Erythrocebus patas)的遗传特征

International Journal of Molecular Biology Open Access Pub Date : 2023-04-18 DOI: 10.15406/ijmboa.2023.06.00145

Grace Oluwatosin Ajayi

{"title":"Genetic characterization of patas monkey (Erythrocebus patas) in new-bussa, niger state, Nigeria","authors":"Grace Oluwatosin Ajayi","doi":"10.15406/ijmboa.2023.06.00145","DOIUrl":"https://doi.org/10.15406/ijmboa.2023.06.00145","url":null,"abstract":"This study was carried out to identify, organize and characterize the Patas monkey (Erythrocebus patas) genome. Minimally invasive technique was used to obtain the blood samples for analysis. The blood samples were taken with the assistance of a professional veterinary doctor. They were transferred immediately to blood tubes which contain 95% ethanol for preservation and then stored in refrigerator before laboratory analysis. Genetic analyses revealed that the mitogenome was 16,678 base pair (bp) in length, with an overall base composition of 31.8% A, 25.8% T, 29.7% C, and 12.7% G. The A+T content (57.6%) was greater than the G+C content (42.4%). Phylogenetic analysis was based on the COI sequences of 9 individuals from different locations. The newly sequenced individuals are from Benin Republic, South West, Nigeria and North Central, Nigeria, West Africa; while other sequences were downloaded from the National Center for Biotechnology Information (NCBI database). The nucleotide diversity shows both the frequency and differences between haplotypes, hence, it is a more suitable parameter to estimate the genetic diversity in populations than haplotype diversity. The Kimura-2-parameter (K2P) was used to estimate the genetic distances among the aligned sequences. The study indicates significant genetic differentiation among all of the geographical locations which indicates high genetic distances. The findings revealed a relatively low frequency polymorphisms and absence of variants of alleles among the population of patas. This exemplified a stable demographic history with a stable population size. Hence, the study provides a useful database for analyzing the phylogenetic relationship of Erythrocebus patas. However, further genetic study is recommended for improved conservation of primates in Nigeria.","PeriodicalId":179796,"journal":{"name":"International Journal of Molecular Biology Open Access","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126036103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Egg quality traits, phenotypic correlations, egg and yolk weights prediction using external and internal egg quality traits of Japanese quails reared in Calabar, Nigeria 利用尼日利亚卡拉巴尔饲养的日本鹌鹑的外部和内部蛋品质状、表型相关性、蛋重和蛋黄重进行预测

International Journal of Molecular Biology Open Access Pub Date : 2020-03-31 DOI: 10.15406/ijmboa.2020.05.00126

Okon B, Ibom La, Dauda A, Ebegbulem Vn

{"title":"Egg quality traits, phenotypic correlations, egg and yolk weights prediction using external and internal egg quality traits of Japanese quails reared in Calabar, Nigeria","authors":"Okon B, Ibom La, Dauda A, Ebegbulem Vn","doi":"10.15406/ijmboa.2020.05.00126","DOIUrl":"https://doi.org/10.15406/ijmboa.2020.05.00126","url":null,"abstract":"Three hundred freshly laid eggs collected from 12 weeks old brown Japanese quails on deep litters fed 18% CP and 2700 kcal ME/kg ration and water ad libitum. External egg quality traits measured included egg (weight and width) and yolk (weight, height and width). Data collected were used to estimate descriptive statistics, phenotypic correlations and to predict egg and yolk weights. Results of phenotypic correlation of external egg traits investigated expressed mostly non-significant (p>0.05) both positive and negative low values with the exceptions of two higher, negative significant (p<0.01) correlations observed between egg weight and egg length (r= – 0.721) and egg weight and shell thickness (r= – 0.616). Similarly, results of phenotypic correlations of internal egg traits expressed mostly non-significant (p>0.05) both positive and negative low values with the exceptions of very high negative correlation coefficient (r= – 0.802) between yolk weight and albumen width and moderate negative correlation coefficient (r= – 0.561) between albumen height and albumen width. The only moderate positive significant correlation (r=0.519) obtained in this study was between yolk weight and albumen height. The regression estimates of parameters and coefficient of determination for simple (one trait) and multiple (two traits and for predicting egg and yolk weight sh6wed very low non-significant associations/interrelationship. But with three to five traits fitted into the multiple linear regression equations, highly significant (p<0.001) associations/interrelationships were obtained for egg and yolk weights. Egg and yolk weights can be predicted using external and internal egg traits measurements with two external traits (egg length and egg width) and two internal traits (albumen height and albumen width) best predicted egg and yolk heights respectively.","PeriodicalId":179796,"journal":{"name":"International Journal of Molecular Biology Open Access","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141219897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2