从鸡蛋样本中分离出的病原体的分子特征

Sagadevan P, Jenifer Christina, Raghunath M, Nagarajan M, Premalatha S, Preeethi R, Janarthanan P
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引用次数: 0

摘要

根据本研究的生化分析(类似于甲基红、Voges-Proskauer、吲哚和柠檬酸盐等一系列生化测试),确定这些微生物为克雷伯氏菌。在本研究中,伊诺拉菌素(50%)和头孢他啶(80%)的耐药率最高,与头孢他啶(85%)和头孢他啶(68%)的最高耐药率相似,其 MIC 值介于 32 至 256 毫克/升之间。本研究中分离出的假单胞菌和克雷伯氏菌质粒的分子量分别为 2.6 kb 和 2.7 kb,这与大多数分离物携带多个质粒的情况相似,质粒大小从约 2 kb 到超过 150 kb 不等。有 79 个分离株含有大于 50 kb 的质粒,其中一些还含有多个大质粒。本研究发现,假单胞菌和克雷伯氏菌的分子量分别为 356 碱基对和 359 碱基对,与通过 PCR 扩增抗消毒基因 qacE1 和 ceA 筛选出的 85 个肺炎克雷伯氏菌临床分离株的分子量相似。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular characterisation of pathogens isolated from egg samples
ceA on the biochemical analysis of this study, which is similar to a battery of biochemical tests including methyl red, Voges-Proskauer, indole and citrate, the organisms were determined to be Klebsiella. In the present study, the highest resistance rate was observed for inoflavuxin (50%) and ceftazidimine (80%), which is similar to the highest resistance rate for cefotaxime (85%) and ceftazidime (68%), with the MIC ranging from 32 to 256 mg/l. The Pseudomonas and Klebsiella plasmid isolation in the present study was found to have a molecular weight of 2.6 kb and 2.7 kb, which is similar to most isolates harboring multiple plasmids ranging in size from about 2 kb to over 150 kb included. Seventy-nine isolates contained plasmids larger than 50 kb, and some contained multiple large plasmids. In the present study, the molecular weight of Pseudomonas and Klebsiella was found to be 356 base pairs and 359 base pairs, respectively, which is similar to that of the 85 clinical isolates of Klebsiella pneumonia screened by PCR amplification of the antiseptic resistance genes qacE1 and ceA.
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