KSBB Journal最新文献_第9页

Separation and Purification of Antimicrobial Substance from Rheum palmatum L. against Vaginitis-inducing Strains 掌大黄抗阴道炎病原菌抗菌物质的分离纯化

KSBB Journal Pub Date : 2021-03-31 DOI: 10.7841/KSBBJ.2021.36.1.16

G. Song, H. Ko, Hyun-Soo Kim

{"title":"Separation and Purification of Antimicrobial Substance from Rheum palmatum L. against Vaginitis-inducing Strains","authors":"G. Song, H. Ko, Hyun-Soo Kim","doi":"10.7841/KSBBJ.2021.36.1.16","DOIUrl":"https://doi.org/10.7841/KSBBJ.2021.36.1.16","url":null,"abstract":"The present study aimed to explore natural substances as an alternative to antibiotics that result in adverse effects, such as reduced counts of essential lactic acid bacteria in the vagina and compromised vaginal function. For this purpose, Rheum palmatum L. methanol extracts were used to separate substances that do not inhibit lactic acid production in the vagina while inhibiting the pathogens causing vaginitis using different methods used in previous studies. Extraction was performed to separate the substance in the R. palmatum L. methanol extract with antibacterial activity against pathogens causing vaginitis. The maximum antibacterial substance was detected in the ethyl acetate layer extract on TLC (Thin Layer Chromatography). Fractionation by silica gel column chromatography to separate the antibacterial substance from the R. palmatum L. ethyl acetate layer extract revealed that fraction 4 had the maximum antibacterial substance. In addition, on analyzing fraction 4 using HPLC, two peaks were identified and separated at Rt 12.372 and Rt 13.44 min, with peak purities of 82.8% and 92.9%, respectively. After concentrating the fractions from both the peaks to 10 mg/mL, their antibacterial activity was tested against pathogens causing vaginitis and a beneficial strain found in the vagina. Inhibitory zones of 7 mm and 6.5 mm against Sphingomonas paucimobilis KCTC 2834 and Proteus mirabilis KCTC 2510, respectively, were identified. No antibacterial activity was found against the vaginal strain Lactobacillus coleohominis KCTC 21007.","PeriodicalId":17865,"journal":{"name":"KSBB Journal","volume":"6 1","pages":"16-22"},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88379625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation of High Salinity Fishery Wastes in a 150-L Reactor by Bacillus licheniformis TK3-Y for Reutilization as Liquid Fertilizer 地衣芽孢杆菌TK3-Y在150-L反应器中降解高盐度渔业废弃物作为液体肥料的研究

KSBB Journal Pub Date : 2020-12-31 DOI: 10.7841/KSBBJ.2020.35.4.288

K. Kang, Hee Jin Lee, Taejin Park, S. Han, kim seung-young

{"title":"Biodegradation of High Salinity Fishery Wastes in a 150-L Reactor by Bacillus licheniformis TK3-Y for Reutilization as Liquid Fertilizer","authors":"K. Kang, Hee Jin Lee, Taejin Park, S. Han, kim seung-young","doi":"10.7841/KSBBJ.2020.35.4.288","DOIUrl":"https://doi.org/10.7841/KSBBJ.2020.35.4.288","url":null,"abstract":"High salinity fishery wastes (HFW) containing seaweed and fish waste cause great influence on the local environment. However, the fishery wastes contain biodegradable components such as carbohydrates, proteins and lipids that are able to be converted to useful bioactive substances by useful bacterium. Therefore, we evaluated large scale biodegradation property of 17.5% of salinity HWF containing green seaweed and anchovy waste by Bacillus licheniformis TK3-Y in 150-L fermenter and utilization of HFW biodegraded product as liquid fertilizer. During 7-day biodegradation, the pH increased with increasing cell density. The decrease in DO (dissolved oxygen) and ORP (oxidation-reduction potential) meant that the HFW was actively biodegraded. CODcr (chemical oxygen demand) and T-N (total nitrogen) were also removed by 87.3 and 87.1%, respectively. In addtion, B.licheniformis TK3-Y showed 0.45 U/mL cellulolytic, 1,016 U/mL proteolytic, and 5.92 U/mL lipolytic activities. The 500and 1000fold diluted supernatant from the culture of HFW had no phytotoxicity. In barley hydroponic culture, the 1,000-fold diluted culture supernatant showed a good fertilizing ability. This study indicates the large-scale treatment of HFW and the biodegraded products are sufficient for agricultural value.","PeriodicalId":17865,"journal":{"name":"KSBB Journal","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84289533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antioxidant and Anti-inflammatory Effects of Machilus thunbergii Leaves and Branch Extracts with Different Extraction Methods 不同提取方法对浙柏叶、枝提取物抗氧化、抗炎作用的影响

KSBB Journal Pub Date : 2020-12-31 DOI: 10.7841/KSBBJ.2020.35.4.310

Nain Kim, Moon-Hee Choi, Shin, Hyun-Jae

引用次数: 0

Development of Ultrasound-assisted Extraction for Production of Bioactive Compounds with Whitening and Anti-wrinkle Effects from Sargassum horneri 马尾藻美白抗皱活性物质超声辅助提取的研究进展

KSBB Journal Pub Date : 2020-12-31 DOI: 10.7841/KSBBJ.2020.35.4.294

D. Gam, J. Hong, S. Jung, Dong-Ho Baek, Kim, Jinw

{"title":"Development of Ultrasound-assisted Extraction for Production of Bioactive Compounds with Whitening and Anti-wrinkle Effects from Sargassum horneri","authors":"D. Gam, J. Hong, S. Jung, Dong-Ho Baek, Kim, Jinw","doi":"10.7841/KSBBJ.2020.35.4.294","DOIUrl":"https://doi.org/10.7841/KSBBJ.2020.35.4.294","url":null,"abstract":"Statistically-based optimization using response surface methodology was performed to derive optimal extraction conditions for the extraction of bioactive compounds with antioxidant activity, skin whitening, and anti-wrinkle effects from Sargassum horneri, marine waste, using the ultrasoundassisted extraction (UAE). The main UAE process variables, extraction time (5.3~18.7 min, X1), extraction temperature (22.4~79.6C, X2), and ethanol concentration (0~99.5% v/v, X3), were selected through preliminary experiments and optimized using central composite design at five levels. Total phenolic content (TPC), total flavonoid content (TPC), DPPH radical scavenging activity (RSA), tyrosinase activity inhibition (TAI), and collagenase activity inhibition (CAI) were analyzed and second-order polynomial models were generated for predicting the responses. The optimum UAE conditions for enhancing antioxidants, skin whitening, and antiwrinkle effects were extraction time of 11.7 min, extraction temperature of 54.8C, and ethanol concentration of 73.0%, under which RSA, TAI, and CAI were 29.1%, 78.5%, and 83.6%, respectively. In this study, we proposed an extraction process for the production of cosmetic materials that have a combination of antioxidant, whitening, and wrinkle effects from S.horneri, which are marine waste.","PeriodicalId":17865,"journal":{"name":"KSBB Journal","volume":"25 1","pages":"294-302"},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86088798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Anti-inflammatory Property of Chicken Feet, Acanthopanax, and Eucommia ulmoides Oliver Mixture Extract in LPS-stimulated RAW 264.7 Macrophage Cells 鸡爪、刺五加和杜仲混合提取物对lps刺激的RAW 264.7巨噬细胞的抗炎作用

KSBB Journal Pub Date : 2020-12-31 DOI: 10.7841/KSBBJ.2020.35.4.330

J. Baek, Kang Ki Sung, Lee Ah Young, H. Kim

{"title":"Anti-inflammatory Property of Chicken Feet, Acanthopanax, and Eucommia ulmoides Oliver Mixture Extract in LPS-stimulated RAW 264.7 Macrophage Cells","authors":"J. Baek, Kang Ki Sung, Lee Ah Young, H. Kim","doi":"10.7841/KSBBJ.2020.35.4.330","DOIUrl":"https://doi.org/10.7841/KSBBJ.2020.35.4.330","url":null,"abstract":"This study was to investigate the anti-inflammatory effect of chicken-feet, Acathopanax, and Eucommia ulmoides Oliver mixture (CAEM) extract against lipopolysaccharide (LPS) in RAW 264.7 macrophage cells. We examined cytotoxicity of CAEM extract on RAW 264.7 cells, and their anti-inflammatory properties by measuring nitric oxide (NO) and pro-inflammatory cytokine production. In addition, inflammation-related gene expression was analyzed by real time-PCR in LPS-stimulated RAW 264.7 macrophage cells. Treatment of CAEM extract at concentrations of 0.31-2.5 mg/ mL did not have any cytotoxic effect in RAW 264.7 cells. Treatment of LPS significantly increased the NO production, whereas CAEM extract (1.25, 2.5 mg/mL) significantly decreased the levels of NO, compared to only LPS-treated cells. Furthermore, treatment with CAEM extract significantly suppressed the LPS-induced interleukin (IL)-6 and tumor necrosis factor-α (TNF-α) production. In order to confirm whether CAEM extract regulates inflammation-related gene expression, we analyzed the mRNA levels of IL-6, TNF-α, IL-1β, and inducible NO synthase (iNOS) by real time-PCR. Exposure of RAW 264.7 cells on LPS upregulated the mRNA levels of IL-6, TNF-α, IL-1β, and iNOS, compared to control group. However, those of expression were downregulated by treatment with CAEM extract. These findings suggest that CAEM extract may contribute to the prevention and treatment of inflammatory-related diseases.","PeriodicalId":17865,"journal":{"name":"KSBB Journal","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83128557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effect of Foot and Mouth Disease Virus Serotype A and O Epitope-constituted Recombinant Protein Vaccine 口蹄疫病毒A型和O型表位重组蛋白疫苗的保护作用

KSBB Journal Pub Date : 2020-12-31 DOI: 10.7841/KSBBJ.2020.35.4.319

M. Park, H. Lee, H. Lee, Dae Seog Lim, S. Moon

引用次数: 0

Production of 3-Hydroxypropionic Acid by Engineered Kluyveromyces marxianus 工程克鲁维酵母生产3-羟基丙酸的研究

KSBB Journal Pub Date : 2020-12-31 DOI: 10.7841/KSBBJ.2020.35.4.337

Jae-Bum Park, kang-kyoung-gon, D. Kwon, S. Ha

引用次数: 1

Research Trend and Product Development Potential of Non-animal Leather 非动物皮革的研究趋势及产品开发潜力

KSBB Journal Pub Date : 2020-12-31 DOI: 10.7841/KSBBJ.2020.35.4.281

S. Heo, Hyun-Jae Shin