The Effects of Gleditsia japonica Extract Following Biorenovation on Melanogenesis-related genes in B16F10 Melanoma Cells

Biorenovation is a method of modifying the structure of a broad range of substrates such as chemical compounds and plant extract by microbial enzymes with the potential benefits of reduced cytotoxicities and enhanced biological activities relative to its parent substrates. The purpose of this study was to generated Gleditsia japonica Miquel leaf (GJ) extract using Biorenovation technology and tested the whitening properties of its Biorenovation product (GJBR) in αmelanocyte-stimulating hormone (α-MSH) stimulated B16F10 melanoma cells. As a result of in vitro tyrosinase activity assay, GJBR showed significant whitening effect than GJ. Especially in B16F10 cells, we found that pre-treatment of GJBR efficiently prevented extracellular secretion of melanin with dose-dependent manner. Therefore, it suggests that maybe various components that converted by biorenovation contained in the GJBR affect multiple factors involved in α-MSH-induced melanogenesis in B16F10 cells. Then together, our results indicate that GJBR could be a new source for the ingredients of cosmetics.