Jundishapur Journal of Medical Sciences最新文献_第7页

Effect of Detraining Type on Telomere Length and TRF1& TRF2 Gene Expression of Skeletal Muscle in C57BL/6 Male Mice 去训练型对C57BL/6雄性小鼠骨骼肌端粒长度及trf1和TRF2基因表达的影响

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-01 DOI: 10.32598/jsmj.21.1.2719

Mostafa Khodadoost, S. Shakerian, S. Arjmand, M. Nikbakht

{"title":"Effect of Detraining Type on Telomere Length and TRF1& TRF2 Gene Expression of Skeletal Muscle in C57BL/6 Male Mice","authors":"Mostafa Khodadoost, S. Shakerian, S. Arjmand, M. Nikbakht","doi":"10.32598/jsmj.21.1.2719","DOIUrl":"https://doi.org/10.32598/jsmj.21.1.2719","url":null,"abstract":"Background and Objective: The aim this study was to investigate the effect of sedentary lifestyle on Telomere system of mice skeletal muscle. Methods: The subjects were C57BL/6 mice (n=24) that were randomly divided into four groups: Base control (n=6), control (n=6), LIIT (n=6), and HIIT (n=6) groups. The exercise includes 5 days a week for 8 weeks and then kept inactive for 4 weeks. The factors were measured after DNA and RNA extraction using Real time-PCR method. The data were evaluated using two-way ANOVA test. Results: The results of this study showed that there wasn’t a significant difference between the expression of TRF1 gene in ST and FT muscles (P=0.825), sedentary lifestyle type (P=0.062) and the interaction between muscle type and sedentary lifestyle type (P=0.408). There was no significant difference between the expression of TRF2 gene in ST and FT muscles (P=0.073), sedentary lifestyle type (P=0.309) and the interaction between muscle type and sedentary lifestyle type (P=0.093). In general, There was no significant difference between the telomere length in ST and FT muscles (P=0.763), sedentary lifestyle type (P=0.053) and the interaction between muscle type and sedentary lifestyle type (P=0.651). Conclusion: Applying sedentary lifestyle types, including inactivity after High and low-intensity exercise, affects the telomere system in skeletal muscle in both muscles the same. It seems that the effect of inactivity in people with an experience of exercise with different intensities does not have a different effect on the type of skeletal muscle tissue.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86941400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbiological and Chemical Properties of Falafel Samples Collected From Street Food Market in Ahvaz, Iran 伊朗阿瓦士街头食品市场沙拉三明治样品的微生物学和化学性质

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-01 DOI: 10.32598/jsmj.21.1.2708

Reza Zadeh-Dabbagh, M. Hashemi, Marzieh Massah, E. Taherian, Naji Sayyahi, Farkhondeh Haghparasti, M. Marhamati, L. Alavi, Nikoo Bahrami, Maliheh Karami, S. M. A. Noori

{"title":"Microbiological and Chemical Properties of Falafel Samples Collected From Street Food Market in Ahvaz, Iran","authors":"Reza Zadeh-Dabbagh, M. Hashemi, Marzieh Massah, E. Taherian, Naji Sayyahi, Farkhondeh Haghparasti, M. Marhamati, L. Alavi, Nikoo Bahrami, Maliheh Karami, S. M. A. Noori","doi":"10.32598/jsmj.21.1.2708","DOIUrl":"https://doi.org/10.32598/jsmj.21.1.2708","url":null,"abstract":"Background and Objectives Falafel is one of the most popular artisanal foods in Iran, especially in Khouzestan province. However, no comprehensive study has been performed to evaluate its microbiological and chemical properties. This study aims to evaluate microbiological and chemical properties of falafel samples collected from street food market in Ahvaz, Iran. Subjects and Methods Microbiological tests including total viable count (TVC), psychrophilic count, Escherichia coli test, staphylococcus aureus test, coliform count, bacillus cereus test, Salmonella test, and mold count were performed to evaluate microbiological properties of falafel samples (cooked and uncooked). The chemical properties were assessed by performing protein test, ash, carbohydrate, lipid, salt, peroxide, and acidity tests. All tests were done according to the methods described by the Institute of Standards & Industrial Research of Iran (ISIRI). Results Microbiological tests revealed that TVC (108 cfu/g), coliform count (3.7×103 cfu/g) and mold count (1.3×103 cfu/g) were significantly higher than the amounts set by the ISIRI (P<0.05), indicating high contamination of uncooked falafel samples. The major problems observed by chemical tests was the high content of lipid (20.11%) and peroxide value (47.85 meq/kg) which were significantly higher than the values set by the ISIRI (P<0.05). Conclusion Microbiological and chemical properties of falafel are poor, indicating the poor hygienic conditions of its preparation in street food market. We recommend more attention to and supervision on the preparation of artisanal foods such as falafel in Iran.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83687813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Effect Of Crocin and Safranal Components of Saffron on Skin Wound Healing in Streptozotocin-Induced Diabetic Rat 藏红花中藏红花素及藏红花酚类成分对链脲佐菌素诱导的糖尿病大鼠皮肤创面愈合的影响

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-01 DOI: 10.32598/jsmj.21.1.2358

Maliha Goli, Saeede Samare Mousavi, R. Rahbarian, Majid Rajabiyan

{"title":"The Effect Of Crocin and Safranal Components of Saffron on Skin Wound Healing in Streptozotocin-Induced Diabetic Rat","authors":"Maliha Goli, Saeede Samare Mousavi, R. Rahbarian, Majid Rajabiyan","doi":"10.32598/jsmj.21.1.2358","DOIUrl":"https://doi.org/10.32598/jsmj.21.1.2358","url":null,"abstract":"Background and Objectives Herbal medicines have fewer side effects than chemical drugs. In this regard, researchers are looking to find herbal ingredients to heal diabetic wounds. Saffron is a powerful antioxidant that can be effective in treating diabetic wounds. This study aims to evaluate the effect of saffron’s main ingredients (crocin and safranal) on skin wound healing in streptozotocin-induced diabetic rats. Subjects and Methods In this experimental study, 30 rats were divided into six groups. One control group, one diabetic group, two diabetic groups treated with crocin (300 and 600 mg/kg body weight), and two diabetic groups treated with safranal (300 and 600 mg/kg body weight). The diabetes was induced by intraperitoneal injection of streptozotocin (55 mg/kg body weight). Sampling was performed for histological studies to measure the number of neutrophils, macrophages, blood vessels, and epithelium thickness on days 3, 7, 15 and 20 after wound creation. Results The collected data were analyzed by one-way analysis of variance and the least significant difference test, considering the significance level of P<0.05. Conclusion Crocin and safranal components of saffron can accelerate the wound healing process in diabetic rats by affecting the number of neutrophils, macrophages, blood vessels, and epithelial thickness.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78570453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing the Recognition of Stop Consonants in Persian Between Adults Aged 30-39 and 40-49 Years 30-39岁成人与40-49岁成人波斯语停辅音识别的比较

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-01 DOI: 10.32598/jsmj.21.1.2354

A. Mohammadzadeh, Ali Dehghan Ahmadabad, Z. Sharafi, Farzad Hamzehpour

{"title":"Comparing the Recognition of Stop Consonants in Persian Between Adults Aged 30-39 and 40-49 Years","authors":"A. Mohammadzadeh, Ali Dehghan Ahmadabad, Z. Sharafi, Farzad Hamzehpour","doi":"10.32598/jsmj.21.1.2354","DOIUrl":"https://doi.org/10.32598/jsmj.21.1.2354","url":null,"abstract":"Background and Objectives Due to the improvement of living conditions in recent decades, there is an important issue called quality of life (QoL). One of the important factors that affect the QoL is the ability to communicate with others. Everyday communication is usually interfered by noise. Consonants play an important role in understanding the meaning of words. Since stop consonants are dependent on the following vowels, they are more vulnerable to the masking effects of noise. The present study aims to evaluate the effect of age, signal-to-noise ratio (SNR), and concurrent vowels on the recognition of stop consonants in Persian. Subjects and Methods The present cross-sectional study was conducted on 60 adults with normal hearing in two age groups of 30-39 and 40-49 years. After the auditory and speech assessments, the recognition of stop consonants in the form of consonant-vowel-consonant syllables and in the presence of babble noise at three SNRs was compared between two age groups. Results The difference in the recognition score of stop consonants was significant between two age groups at the SNRs of 0, -5, and -10. There was also a significant difference between the two age groups regarding the recognition of stop consonants in the presence of some vowels at three SNRs. Conclusion With increasing age, the recognition ability of stop consonants in the presence of babble noises decreases. Increasing noise also reduces the recognition of stop consonants, especially those with some vowels.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74118254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Three Different Types Of Recovery After Intensive Training on the Aldolase Level and Some Hematological Indicatorsin Female Runners 高强度训练后三种不同恢复方式对女性跑步者醛缩酶水平及一些血液学指标的影响

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-01 DOI: 10.32598/jsmj.21.1.2711

M. Rahmani Ghobadi, Seyyed Ali Hoseini, Ghobad Hasanpour

{"title":"Effect of Three Different Types Of Recovery After Intensive Training on the Aldolase Level and Some Hematological Indicatorsin Female Runners","authors":"M. Rahmani Ghobadi, Seyyed Ali Hoseini, Ghobad Hasanpour","doi":"10.32598/jsmj.21.1.2711","DOIUrl":"https://doi.org/10.32598/jsmj.21.1.2711","url":null,"abstract":"Background and Objectives The aim of this study was to Acute changes in aldolase activity and some hematologic parameters compared to different recoveries. Subjects and Methods The research is of applied type and quasi-experimental research method which was conducted in field-laboratory form with pre-test-post-test design with control group on female semiprofessional athletes in athletics (runners) in Tehran. Sampling was done by simple random sampling, so that after informing and inviting interested people and passing the preliminary stages, 30 runners were selected as the research sample and randomly in 3 groups of 10 people (active recycling group (running), inactivated recycling group (sports massage) and inactive sitting). The main activity included a sports competition. Blood samples were taken from the samples in 3 stages (pre-test (fasting), post-race and post-recovery). In this study, one-way analysis of variance and SPSS statistical software v. 22 were used to examine the data at a significance level of P<0.05. Results The results of this study showed that there is no significant difference between the three types of recycling methods in changing the amount of aldolase and erythrocyte factors (P>0.05), but recycling by massage has an effect on reducing aldolase levels after strenuous exercise. There is a significant difference between the three types of recycling methods (passive, jogging, massage) in reducing the number of white blood cells and hemoglobin in favor of recovery by the massage group. Conclusion According to the results, it can be said that among the recovery methods, the use of massage was more effective in reducing the aldolase activity of female athletes after the run competition.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"PP 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84165059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Teaching the Use of Inhaler to a Family Caregiver on its Correct Use By the Elderly Patients: A Randomized Clinical Trial 向家庭护理人员教授吸入器使用对老年患者正确使用的影响:一项随机临床试验

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-01 DOI: 10.32598/jsmj.21.1.2251

Roghayeh Eslami, H. Ebrahimi, Solmaz Talebi, Mehdi Mansouri, Hossein Bagheri

{"title":"The Effect of Teaching the Use of Inhaler to a Family Caregiver on its Correct Use By the Elderly Patients: A Randomized Clinical Trial","authors":"Roghayeh Eslami, H. Ebrahimi, Solmaz Talebi, Mehdi Mansouri, Hossein Bagheri","doi":"10.32598/jsmj.21.1.2251","DOIUrl":"https://doi.org/10.32598/jsmj.21.1.2251","url":null,"abstract":"Background and Objectives Due to the high rate of inhalers’ incorrect use by the elderly, this study aims to evaluate the effect of teaching the use of inhalers to a family caregiver on its correct use by the elderly patients. Subjects and Methods This is a parallel-group randomized clinical trial on 80 older people with a lung disease aged >60 years who use the inhaler incorrectly and their family caregivers. Participants were randomly assigned to group A (education) and group B (control) using a block randomization method (4×4 blocks). In group A, teaching of the correct use of inhaler was provided to the caregivers of the elderly orally and practically in one session, while in group B, training was provided only to the elderly. Three weeks later, the two groups were evaluated using a researcher-made inhaler use checklist. Results There was no significant difference in the use of inhaler before and after the intervention in group A (2.58±1.26) and B (2.55±1.22) (P<0.05). Pearson correlation test results showed a significant decrease in the correct use of inhaler with the increase of patients’ age (r=-0.24). Conclusion Further studies on the use of family members in elderly patients care, various educational programs by health care providers, and periodic evaluation of the performance of the elderly regarding the use of inhalers are recommended.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90215596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Functional Exercise Along With Online Nutritional Education on Inflammatory Biomarkers in Children With Autism Spectrum Disorder During Covid-19 Pandemic: A Randomized Clinical Trial 功能锻炼和在线营养教育对Covid-19大流行期间自闭症谱系障碍儿童炎症生物标志物的影响:一项随机临床试验

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-01 DOI: 10.32598/JSMJ.21.1.2724

Kimia Moiniafshari, M. Gholami, Hamid Dalvand, M. Effatpanah, Saeed Rezaei

{"title":"The Effect of Functional Exercise Along With Online Nutritional Education on Inflammatory Biomarkers in Children With Autism Spectrum Disorder During Covid-19 Pandemic: A Randomized Clinical Trial","authors":"Kimia Moiniafshari, M. Gholami, Hamid Dalvand, M. Effatpanah, Saeed Rezaei","doi":"10.32598/JSMJ.21.1.2724","DOIUrl":"https://doi.org/10.32598/JSMJ.21.1.2724","url":null,"abstract":"Background and Objectives Autism spectrum disorder (ASD) is a neuro-developmental disorder which is mostly caused by deficits in social interactions. Lack of physical activity and poor nutritional habits are common problems in these patients which may be exaggerated by the Covid-19 pandemic. The study aims to assess the effect of functional training along with online nutrition education on inflammatory biomarkers in children with ASD. Subjects and Methods In this randomized controlled clinical trial, 80 children with ASD (age=9.73±1.29 years, weight=49.94±2.08 kg, height=146.08±40 cm, body mass index=24.71 ±1.48 kg/m2) were randomly divided into four groups of training, education, training+ education, and control. The interventions lasted for 8 weeks. The inflammatory biomarkers including white blood cell (WBC) count, C-reactive protein (CRP) level, neutrophil count, eosinophil count, and basophil count were assessed (using blood samples collected from antecubital vein) before and after the interventions. Results There was no significant difference between the groups before the interventions (P˃0.05). After the intervention, the results showed a significant decrease in WBC (P˂0.001), CRP (P=0.001), neutrophils (sig.=0.009), and eosinophil (P=0.003) in all groups. Basophil count decreased in all groups (P=0.01) except in the education group. Conclusion Functional training and online nutrition education are beneficial interventions for management of inflammatory biomarkers in children with ASD which can be used during the Covid-19 pandemic.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77275042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing of The Effect of Long-Term Caffeine Consumption Combined With Aerobic Exercise on Serum Interleukin-6 Level and Body Composition in Obese Men 长期咖啡因摄入与有氧运动对肥胖男性血清白细胞介素-6水平及体成分影响的比较

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-01 DOI: 10.32598/jsmj.21.1.2481

Jamal Rasoulpour, Hadi Gharani, Aziz Zinvand Lourestani, V. Taeid, MohammadReza Zoalfaghari, Najmedin Espandar, Jalal Shirazd

{"title":"Comparing of The Effect of Long-Term Caffeine Consumption Combined With Aerobic Exercise on Serum Interleukin-6 Level and Body Composition in Obese Men","authors":"Jamal Rasoulpour, Hadi Gharani, Aziz Zinvand Lourestani, V. Taeid, MohammadReza Zoalfaghari, Najmedin Espandar, Jalal Shirazd","doi":"10.32598/jsmj.21.1.2481","DOIUrl":"https://doi.org/10.32598/jsmj.21.1.2481","url":null,"abstract":"Background and Objectives Caffeine is a purine alkaloid with anti-oxidant and anti-inflammatory effects. This study aims to evaluate the effect of long-term caffeine consumption and aerobic exercise on serum interleukin-6 (IL-6) level and body composition in obese men. Subjects and Methods In this quasi-experimental study, 40 obese men (Mean age=32.8±2.74 years, body mass index=30.98±1.37 kg/m2) were randomly divided into four Group of 10 including placebo, supplementation, placebo + exercise, and supplementation + exercise. The aerobic exercise program included running for 8 weeks, 3 sessions per week, each for 25-45 minutes with an intensity of 55-85% of the maximum heart rate. The supplementation group received 500 mg caffeine daily and the placebo group received 500 mg capsules of 2% maltodextrin. Blood samples were taken following 12 hours of fasting before and 48 hours after the last training session to measure IL-6 level. Data were analyzed using oneway ANOVA and Tukey’s post hoc test. The significance level was set 0.05. Results In the exercise+supplementation and placebo+exercise groups significant decreases were reported in the serum level of IL-6 compared to the supplementation and placebo groups in the post-test phase (P<0.05). There was also a significant decrease in body composition of groups placebo+exercise, exercise+supplementation, and supplementation compared to placebo group in the post-test phase (P<0.05). Conclusion Aerobic exercise alone and in combination with long-term caffeine consumption can reduce IL-6 level and improve body composition in obese people.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74237952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The First Endoscopic Diverticulectomy for a Large Zenker’s Esophageal Diverticula in Southwest of Iran: A Case Report 伊朗西南部首次内镜下憩室切除术治疗一个较大的Zenker食管憩室1例报告

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-01 DOI: 10.32598/jsmj.21.1.2667

P. Alavinejad, Azam Sattari, S. Mohammadi

引用次数: 0

Effect of Alpha and Beta Adrenergic Receptors on the Expression of Stk4, Caspase-3, and Sox2 Genes in Neural Stem Cells Derived From the Hippocampus and Treated With Norepinephrine in Rats α和β肾上腺素受体对大鼠海马神经干细胞Stk4、Caspase-3和Sox2基因表达的影响

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-01 DOI: 10.32598/jsmj.21.1.2815

Zakiye Davar, I. Jafari Anarkooli, Mohammadjavad Fridoni, A. Abdanipour

{"title":"Effect of Alpha and Beta Adrenergic Receptors on the Expression of Stk4, Caspase-3, and Sox2 Genes in Neural Stem Cells Derived From the Hippocampus and Treated With Norepinephrine in Rats","authors":"Zakiye Davar, I. Jafari Anarkooli, Mohammadjavad Fridoni, A. Abdanipour","doi":"10.32598/jsmj.21.1.2815","DOIUrl":"https://doi.org/10.32598/jsmj.21.1.2815","url":null,"abstract":"Background and Objectives Uncontrolled stress affects hippocampal-dependent memory through altering the morphology and the proliferation rate of hippocampal progenitor cells. Subjects and Methods In this experimental study, neural stem cells (NSCs) were isolated from the hippocampus of newborn rats and cultivated in a serum-free medium to generate neurosphere. To confirm the induced NSCs, immunocytochemistry and antibody nestin were used. The rate of cell proliferation and cytotoxicity caused by norepinephrine were measured by the MTT assay. NSCs were assigned in the following groups: Control (untreated NSCs), norepinephrine (NSCs treated with norepinephrine), propranolol (NSCs treated with beta receptor blocker propranolol plus norepinephrine), prazosin (NSCs treated with Alfa receptor blocker prazosin plus norepinephrine), and propranolol/ prazosin (NSCs treated with both propranolol and prazosin plus norepinephrine). Real-time PCR was conducted to measure the expression levels of Stk4, Caspase-3 and Sox2 genes. Results The flow cytometry study revealed that NSCs were nestin positive. Real-time PCR results showed that the expression level of Sox2 gene increased by norepinephrine. In addition, the expression level of Stk4 and Caspase-3 genes increased in the groups treated with prazosin. Conclusion The effect of norepinephrine on hippocampus-derived NSCs is receptor-dependent. The increase of norepinephrine under chronic stress can lead to either cell proliferation or apoptosis in NSCs; it acts as a double-edged sword.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75972751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0