Jundishapur Journal of Medical Sciences最新文献_第6页

Evaluation of different biochemical factors in patients with coronavirus in Imam Khomeini Hospital, Selseleh, Lorestan Province 洛勒斯坦省Selseleh伊玛目霍梅尼医院冠状病毒感染患者不同生化指标评价

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-06 DOI: 10.32598/jsmj.20.6.2254

S. Bahramikia, Parvaneh Hemmati Hassan Gavyar, S. Amiri

{"title":"Evaluation of different biochemical factors in patients with coronavirus in Imam Khomeini Hospital, Selseleh, Lorestan Province","authors":"S. Bahramikia, Parvaneh Hemmati Hassan Gavyar, S. Amiri","doi":"10.32598/jsmj.20.6.2254","DOIUrl":"https://doi.org/10.32598/jsmj.20.6.2254","url":null,"abstract":"Coronaviruses, scientifically known as Coronaviruses, are a large family of viruses and subtypes of coronaviruses that range from the common cold virus to the cause of more serious illnesses such as SARS, Mers, and Covid. Symptoms of a new coronavirus that causes COVID-19 usually begin a few days after a person becomes infected with the virus. But in some people, the symptoms may appear a little later. According to statistics and research, symptoms can include fever (in 43.8% of people at admission and 88.7% in hospitalized), dry cough and shortness of breath. In addition to these clinical symptoms, after exposure to the virus, a series of blood factors change, and the measurement of these factors helps to treat and prevent the progression of the disease. The aim of this study was to investigate the changes in various blood factors in coronary patients admitted to Imam Khomeini Hospital in Selseleh, Lorestan province from Feb 20, 2020 to April 3, 2020. In this study, biochemical tests of 50 coronary patients, including 30 males and 20 females ranging in age from 30 to 90 years, were performed. Factors examined included WBC, RBC, hemoglobin, platelets, lymphocytes, ESR, CRP, LDH, and CKMB. The results showed that among the mentioned factors, the amount of lymphocytes in all patients decreased compared to normal range and the amount of ESR, CRP, CKMB and LDH factors increased compared to normal range.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90727221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in insulin-like growth factor 1 and quadriceps muscle size in follicular stage compared to luteal stage in adaptation to resistance training in young women 胰岛素样生长因子1和股四头肌大小在卵泡期和黄体期适应阻力训练的变化

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-06 DOI: 10.32598/jsmj.20.6.2447

H. Masjedi, H. Rajabi, P. Motamedi

{"title":"Changes in insulin-like growth factor 1 and quadriceps muscle size in follicular stage compared to luteal stage in adaptation to resistance training in young women","authors":"H. Masjedi, H. Rajabi, P. Motamedi","doi":"10.32598/jsmj.20.6.2447","DOIUrl":"https://doi.org/10.32598/jsmj.20.6.2447","url":null,"abstract":"Introduction: Menstrual cycle may affect the training of strength and muscle size. The aim of the present study was to determine the effect of exercise volume distribution in the 2 follicular and luteal phases of the menstrual cycle on serum IGF-1 and muscle mass in women. Materials and Methods: 20 women with a mean age of 22 ± 5 years, without contraceptive use and a history of eight weeks of resistance training were selected. Each subject had 8 training sessions on one leg in the first 14 days of menstruation and 2 training sessions on the second 14 days of menstruation (fruiting in the follicular period) and the other leg had 2 training sessions on the first 14 days of menstruation and 8 training sessions on the second 14 days of menstruation (Fertility in the luteal period). Results: Muscle diameter in follicular exercise (0.67 ± 0.34 cm) was higher than luteal exercise (0.46. 0.41 cm). Also, insulin-like growth factor increased in both groups (13%) regardless of the type of exercise schedule. Conclusion: Therefore, although fluctuations in sex hormones during the menstrual cycle may affect muscle growth, but this study showed that at least this different muscle growth is not due to an increase in insulin-like growth factor 1. As a result, it is recommended that menopausal women who do not take birth control pills schedule their strength training based on their individual menstrual cycle.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85458805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The effect of an aerobic exercise period on levels of depression and interleukin-6 cytokine during pregnancy and postpartum in NMRI mice NMRI小鼠孕期和产后有氧运动对抑郁和白细胞介素-6细胞因子水平的影响

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-06 DOI: 10.32598/jsmj.20.6.2525

Fatemeh Yegaaneh, S. Naghibi, M. Vatandoust, A. Zare

{"title":"The effect of an aerobic exercise period on levels of depression and interleukin-6 cytokine during pregnancy and postpartum in NMRI mice","authors":"Fatemeh Yegaaneh, S. Naghibi, M. Vatandoust, A. Zare","doi":"10.32598/jsmj.20.6.2525","DOIUrl":"https://doi.org/10.32598/jsmj.20.6.2525","url":null,"abstract":"Background & Objective: The aim of this study was to investigate the effect of an aerobic exercise period on levels of depression and IL-6 concentration during pregnancy and postpartum in NMRI female mice. Methods: In this experimental and baseline study, 40 female NMRI mice were divided into four groups: Non Exercise-Pregnancy (NE,Preg), Non Exercise-Postpartum (NE,PostP), Exercise-Pregnancy (E,Preg), Exercise-Postpartum (E,PostP). After seeing the vaginal plaque and confirming the day of pregnancy of the animals, two running wheels were placed inside the cage of each pregnant animal for aerobic activity. In order to induce depression during pregnancy and postpartum, pregnant mice from day 5 to 19 of pregnancy were exposed to stress for three sessions a day, and to assess depression levels, the rate of delay in access to food after 24 hours of food deprivation was measured in seconds. Results: moderate and low-intensity aerobic exercise during pregnancy as well as in the postpartum period led to a significant decrease in levels of depression and concentration of interleukin-6 as an inflammatory cytokine in trained mice compared to sedentary mice. Conclusion: As the results of the present study showed, the aerobic activity used was able to reduce the levels of depression and the concentration of inflammatory factor IL-6 during pregnancy and postpartum. Therefore, low-intensity aerobic activity can probably be used as a non-pharmacological intervention during this period to reduce depressive symptoms and improve immune function.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73895047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of body composition in lung function and the effect of exercise training on lung function in adolescent girls with different body mass index

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-06 DOI: 10.32598/jsmj.20.6.2494

Mahsa Farpoor, F. Nazem

{"title":"The role of body composition in lung function and the effect of exercise training on lung function in adolescent girls with different body mass index","authors":"Mahsa Farpoor, F. Nazem","doi":"10.32598/jsmj.20.6.2494","DOIUrl":"https://doi.org/10.32598/jsmj.20.6.2494","url":null,"abstract":"Background and Aim: Background: Obesity and a sedentary lifestyle factors related to pulmonary function and cardio-respiratory requirements. The aim of this study was to determine the effect of regular aerobic training on cardiac and respiratory function in adolescent girls with different body mass index. Methods: Methods: In the present quasi-experimental study, which was conducted with pre-test and post-test design, 45 female students in the age range of 13 to 18 years old were selected and added to 3 groups of 15 people with normal, overweight and obese body mass index. The training protocol included 10 weeks of aerobic training, 3 sessions per week and each exercise session for 20-45 minutes of. Pulmonary function was measured by spirogram and VO2max by modified treadmill bulk test. Results: There were significant differences in FVC, FEV1, PEF, FEV1 / FVC and VO2max in groups with normal body mass index, overweight and obese (P <0.001). After the training period, a significant improvement was observed in spirometry indices (FVC, FEV1, PEF FEV1 / FVC) and VO2max compared to the pre-test (P <0.001), but still significantly lower than the post-test values in the was normal group. Also significant decreased was observed in BMI in obese and overweight groups (P <0.001). Conclusion: The results of the present study showed that obesity is one of the effective factors in reducing pulmonary function and aerobic exercise improves pulmonary function and cardiorespiratory endurance in adolescent girls with different body mass index.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"59 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73101866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the complications and recurrence rate after herniorrhaphy by open and laparoscopic methods in pediatric patients: A case-control study 儿科开放性和腹腔镜疝修补术后并发症和复发率的比较:一项病例对照研究

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-06 DOI: 10.32598/jsmj.20.6.2344

Mehran Peyvaste, S. Askarpour, Shaghayegh Sherafatmand, Mazdak Toghyani Dolatabadi

{"title":"Comparison of the complications and recurrence rate after herniorrhaphy by open and laparoscopic methods in pediatric patients: A case-control study","authors":"Mehran Peyvaste, S. Askarpour, Shaghayegh Sherafatmand, Mazdak Toghyani Dolatabadi","doi":"10.32598/jsmj.20.6.2344","DOIUrl":"https://doi.org/10.32598/jsmj.20.6.2344","url":null,"abstract":"Introduction: Indirect inguinal hernias in children are causing by the opening of the vaginal process and the entry of viscera into it. To compare the rate of complications and recurrence after open and laparoscopic hernia surgery. Methods: In this case-control study, the patients who underwent surgery by laparoscopic and open methods for 2018-2020 years. The data were extracted from a checklist based on the variables sex, age, infection rate, side effect, hydrocele rate, scar length, testicular swelling rate, and rate and recurrence. Results: A total of 261 patients were examined. The results showed that there was a statistically significant difference between the time of surgery in both surgical methods (Z = -9.903, p < 0.001). There is a significant statistical relationship between recurrence and surgical procedure (p = 0.021). None of the patients in the study had a positive infection, positive hydrocele, and positive testicular swelling after surgery. Conclusion: Both methods are safe that can be selected according to the patient's condition. It is recommended that further clinical studies be performed to evaluate and compare these two surgical procedures.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81261256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

evaluation of contrast alternation efficacy in diagnosis of vertical root fracture in conventional digital radiography 传统数字摄影造影剂交替诊断根垂直骨折的疗效评价

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-06 DOI: 10.32598/jsmj.20.6.2312

Ali Habibi Kia, Mohammadamin Javaheri, A. dabaghi, M. Sabaghian

{"title":"evaluation of contrast alternation efficacy in diagnosis of vertical root fracture in conventional digital radiography","authors":"Ali Habibi Kia, Mohammadamin Javaheri, A. dabaghi, M. Sabaghian","doi":"10.32598/jsmj.20.6.2312","DOIUrl":"https://doi.org/10.32598/jsmj.20.6.2312","url":null,"abstract":"Background and objective: Most digital imaging systems today offer a variety of image processing techniques. Due to the importance of the accuracy of this imaging and its widespread use in this study we investigate the effect of contrast change in the diagnosis of vertical root fracture in conventional digital radiography. Materials and methods: in this in vitro study 24 human single-rooted teeth induced vertical root fracture with underwent vrf. Digital images were prepared before and after induction vrf. Images with three low, medium and high contrasts were examined by two radiologists.Data were tested using kappa and MacNemar tests in SPSS software. Overall accuracy was calculated using Roc curve analyzes. Results: The highest sensitivity specificity and overall accuracy are related to images with moderate contrast (95.83%, 100% and 98% in the first observer and 100%, 100% and 100% in the second observer, respectively). In case of low and high contrast to detect vertical root fracture, there is no statistically significant difference with reality (p<0/001). Conclusion: Medium contrast can be used as a processing filter in the detection of vertical root fractures.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85941017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation and comparison of the antioxidant activity of extractable and non-extractable polyphenols of sour orange peel 酸橙皮可提取与不可提取多酚抗氧化活性的评价与比较

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-06 DOI: 10.32598/jsmj.20.6.2133

Zahra Rezaei, A. Siahpoosh

{"title":"Evaluation and comparison of the antioxidant activity of extractable and non-extractable polyphenols of sour orange peel","authors":"Zahra Rezaei, A. Siahpoosh","doi":"10.32598/jsmj.20.6.2133","DOIUrl":"https://doi.org/10.32598/jsmj.20.6.2133","url":null,"abstract":"Purpose: Research on food polyphenols has experienced a huge development during the last century. Most studies on polyphenolic compounds have focused on extractable portions. However, an important polyphenolic fraction is ignored (non-extractable polyphenols) because they are trapped in the corresponding extraction residues. In this study, the antioxidant activity of extractable and non-extractable polyphenols of orange peel, extracted by aqueous solvent and acid hydrolysis (with two different solvent systems) respectively, are evaluated and compared. Method: Antioxidant activity was evaluated by four tests of radical inhibition of 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP) and iron chelating. The amount of polyphenolic compounds, flavonoids, oligomeric proanthocyanidins, and flavanones were respectively determined by folin-ciocalteu, AlCl3 and UV-visible spectrophotometer. Also, the anti-lipid peroxidation effect in rat liver was measured. Results: The results of antioxidant analysis showed that non-extractable polyphenolic extract, which extracted by methanol / sulfuric acid solvent had the highest level of antioxidant activity in sour orange peel. It can be due to the high polyphenolic compounds in this extraction compared to the others. Conclusion: All extracts had antioxidant activity and can be suggested as a potential natural source in food and pharmaceutical industries.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82352309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measurement of lead concentration in the blood of patients undergoing appendectomy and cholecystectomy 阑尾和胆囊切除术患者血铅浓度的测定

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-06 DOI: 10.32598/jsmj.20.6.2304

K. Kazemnia

{"title":"Measurement of lead concentration in the blood of patients undergoing appendectomy and cholecystectomy","authors":"K. Kazemnia","doi":"10.32598/jsmj.20.6.2304","DOIUrl":"https://doi.org/10.32598/jsmj.20.6.2304","url":null,"abstract":"Introduction:Lead poisoning is a life-threatening condition due to its acute and chronic effects.Therefore, preventive methods are very important to prevent the multiple toxic effects of this metal.Due to the lethal effects of lead and its increasing concentration in the environment, this study was designed and performed to measure the concentration of lead in the blood and tissue of patients undergoing appendectomy and cholecystectomy. Methods andmaterials: In a cross-sectional study that was performed on patients with appendectomy or cholecystectomy in Razi Hospital in Ahvaz during a period of twelve months from May 2019 to May 2020. We examined 120 patients in three groups of appendectomy (n = 40), cholecystectomy (n = 40) and control (n = 40).Blood samples were taken from all three groups. Then, after the operation, a pathology sample was taken from the appendix or gallbladder from patients in two groups who underwent appendectomy and cholecystectomy. X-ray imaging was used to check for lead. Results: Our results show well the mean blood lead concentration in the appendectomy group In the cholecystectomy group and in the control group, the difference between the three groups was statistically significant (<0.001). Also, lead was found in 21 samples(52.5%)from the appendectomy group and lead in 23 samples(57.5%) from the cholecystectomy group, but this difference was not statistically significant. ( p= 0.175). Conclusion: Based on the results of this study, it can be said that chronic exposure and lead poisoning may play a role in the occurrence and change of gallstones and appendicitis.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87987956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of Primary Immunodeficiency Diseases and Related Risk Factors Among Children Attending a Hospital in Ahvaz, Iran 在伊朗阿瓦士一家医院就诊的儿童中原发性免疫缺陷疾病的患病率及相关危险因素

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-01 DOI: 10.32598/jsmj.21.1.2273

F. Abolnezhadian, S. Iranparast, Bahareh Vojouhi

{"title":"Prevalence of Primary Immunodeficiency Diseases and Related Risk Factors Among Children Attending a Hospital in Ahvaz, Iran","authors":"F. Abolnezhadian, S. Iranparast, Bahareh Vojouhi","doi":"10.32598/jsmj.21.1.2273","DOIUrl":"https://doi.org/10.32598/jsmj.21.1.2273","url":null,"abstract":"Background and Objectives Primary immunodeficiency (PI) diseases are those occur because of a primary disorder in the immune system. Subjects and Methods This retrospective study was conducted on 70 children with PI diseases referred to the allergy and immunology department of Abuzar Children Hospital in Ahvaz, Iran from 2015 to 2018. Results The most common PI diseases in the majority of male children (56.92%) were severe combined immunodeficiency disease (21.42%) and chronic granulomatous disease (8.57%). Parents in most of patients (83%) were blood relatives. About 66.67% of all death cases (9.23%) was due to severe combined immunodeficiency disease; hypogammaglobulinemia and Griscelli syndrome accounted for about 16.67% of deaths. The most common manifestations were pneumonia, fever, diarrhea, and sepsis. Based on the family history, 33 children had allergies, 9 had malignancy, one had autoimmune disease, and 39 had mothers with a history of abortion. Most of children were under treatment with intravenous immunoglobulin and prophylactic antibiotics, (99.88% and 88.99%, respectively), while 2.81% were under interferon-gamma therapy and transplantation, and 1.40% were receiving corticosteroids and granulocyte colony-stimulating factor therapy. Conclusion The severe combined immunodeficiency disease, chronic granulomatous disease, and Bruton disease are the most common PI diseases in children living in Ahvaz.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"161 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73697084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-Vitro Evaluation of Anticancer and Antibacterial Properties of Pseudocerastes Persicus Snake Venom Fractions 伪槐蛇毒部分抗癌和抗菌性能的体外评价

Jundishapur Journal of Medical Sciences Pub Date : 2022-03-01 DOI: 10.32598/jsmj.21.1.1846

J. Zargan, Majid Mirzaei Nodushan, H. Sobati, Ashkan Haji Noormohammadi, H. Goodarzi, F. Ebrahimi

{"title":"In-Vitro Evaluation of Anticancer and Antibacterial Properties of Pseudocerastes Persicus Snake Venom Fractions","authors":"J. Zargan, Majid Mirzaei Nodushan, H. Sobati, Ashkan Haji Noormohammadi, H. Goodarzi, F. Ebrahimi","doi":"10.32598/jsmj.21.1.1846","DOIUrl":"https://doi.org/10.32598/jsmj.21.1.1846","url":null,"abstract":"Background and Objectives In recent years the isolation of effective molecules from snake venom as drug to treatment of some incurable diseases is considered by many biological research centers. The aim of this investigation was to determine electrophorotic and chromatographic patterns of Pseudocerastes persicus venom and in-vitro study of bactericidal and anti-cancer properties of its fractions in human hepatocellular liver carcinoma cell line (HepG2). Subjects and Methods The fractions of venom were isolated and collected using preparative RP-HPLC. The Bactericidal activity of fractions in 20μg/ml of protein concentration was investigated toward Gramnegative and Gram-positive bacteria using MTT and MIC assays. Then, the cytotoxic effects of fractions with higher antibacterial properties were measured by MTT reduction, neutral uptake and comet assays following exposure of HepG2 cancer cells to 20 and 40 μg/ml of protein concentration. Results SDS-PAGE pattern of the crude venom revealed 10 major bands with molecular weight ranging from 13.2 to 99.25 kDa and the separation of fractions from the venom by HPLC resulted in collection of 11 different fractions. The results of this study showed that two fractions (4 & 8) have a significant antibacterial activity against Gram-positive as compared to Gram- negative bacteria. The study of the anti-cancer effect of these two fractions also showed that fraction 8 was more toxic in HepG2 cells. Conclusion We for the first time reported bactericidal and anti cancer effects of isolated fractions of Persian horned viper venom. These properties make components of these fractions a favorable source for isolation of molecule (s) with antibacterial and anti tumor activities.","PeriodicalId":17808,"journal":{"name":"Jundishapur Journal of Medical Sciences","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75826646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1