Effect of Detraining Type on Telomere Length and TRF1& TRF2 Gene Expression of Skeletal Muscle in C57BL/6 Male Mice

Abstract

Background and Objective: The aim this study was to investigate the effect of sedentary lifestyle on Telomere system of mice skeletal muscle. Methods: The subjects were C57BL/6 mice (n=24) that were randomly divided into four groups: Base control (n=6), control (n=6), LIIT (n=6), and HIIT (n=6) groups. The exercise includes 5 days a week for 8 weeks and then kept inactive for 4 weeks. The factors were measured after DNA and RNA extraction using Real time-PCR method. The data were evaluated using two-way ANOVA test. Results: The results of this study showed that there wasn’t a significant difference between the expression of TRF1 gene in ST and FT muscles (P=0.825), sedentary lifestyle type (P=0.062) and the interaction between muscle type and sedentary lifestyle type (P=0.408). There was no significant difference between the expression of TRF2 gene in ST and FT muscles (P=0.073), sedentary lifestyle type (P=0.309) and the interaction between muscle type and sedentary lifestyle type (P=0.093). In general, There was no significant difference between the telomere length in ST and FT muscles (P=0.763), sedentary lifestyle type (P=0.053) and the interaction between muscle type and sedentary lifestyle type (P=0.651). Conclusion: Applying sedentary lifestyle types, including inactivity after High and low-intensity exercise, affects the telomere system in skeletal muscle in both muscles the same. It seems that the effect of inactivity in people with an experience of exercise with different intensities does not have a different effect on the type of skeletal muscle tissue.