Biomedicine and Chemical Sciences最新文献_第2页

Molecular Identification of Four Eruca Sativa L. Cultivars using RAPD Markers 利用RAPD标记对4个油菜品种进行分子鉴定

Biomedicine and Chemical Sciences Pub Date : 2023-07-01 DOI: 10.48112/bcs.v2i3.564

Rusul Abd Al Mahdi Faisal Al Kufee, Attyaf Jameel Thamir

引用次数: 0

Boron Concentration Measurement in Well Water 井水硼浓度测定

Biomedicine and Chemical Sciences Pub Date : 2023-07-01 DOI: 10.48112/bcs.v2i3.520

M. A. Algrifi, T. Salman

引用次数: 0

Indirect Spectrophotometric Method for Determination of Methyldopa in Pure and Pharmaceutical Formulation 间接分光光度法测定纯制剂和制剂中甲基多巴的含量

Biomedicine and Chemical Sciences Pub Date : 2023-04-01 DOI: 10.48112/bcs.v2i2.380

S. Fathi

引用次数: 0

Biomarkers are Significant Clinical Features in Obsessive-Compulsive Disorder 生物标志物是强迫症的重要临床特征

Biomedicine and Chemical Sciences Pub Date : 2023-04-01 DOI: 10.48112/bcs.v2i2.349

A. Mohammadi

引用次数: 0

Autoimmune Polyglandular Syndrome Type 1 自身免疫性多腺综合征1型

Biomedicine and Chemical Sciences Pub Date : 2023-04-01 DOI: 10.48112/bcs.v2i2.452

Haitham S. El Messallamy

引用次数: 0

The Role of Gold Nanoparticles/Au-PEG-PAMAM as Drug Delivery System for Treatment of Breast Cancer 金纳米颗粒/Au-PEG-PAMAM作为治疗乳腺癌的药物传递系统的作用

Biomedicine and Chemical Sciences Pub Date : 2023-04-01 DOI: 10.48112/bcs.v2i2.456

Murtadha M-Hussein A-kadhim, Ali Kelkawi

{"title":"The Role of Gold Nanoparticles/Au-PEG-PAMAM as Drug Delivery System for Treatment of Breast Cancer","authors":"Murtadha M-Hussein A-kadhim, Ali Kelkawi","doi":"10.48112/bcs.v2i2.456","DOIUrl":"https://doi.org/10.48112/bcs.v2i2.456","url":null,"abstract":"To enhance the cellular uptake and chemotherapeutic efficacy of a current chemotherapeutic medication, a nanoparticle drug carrier technology has been designed. Due to their distinctive electrical and optical characteristics, gold nanoparticles (Au NPs) have recently demonstrated intriguing medical and military uses. In the event that they come into touch with a biological system, little is known about their biocompatibility. Metallic nanoparticles have been successfully utilized for a kind of biological applications. A drug delivery system known as Au – PEG – PAMAM – DOX was produced by conjugating the dendrimer with the anti-cancer chemical doxorubicin (DOX) via an amide bond. The amount of DOX released from Au – PEG – PAMAM – DOX at a natural pH was negligible, but this amount significantly increased in an environment with a weak acidic milieu, according to studies on the release of medicines from acellular sources. A research into the intracellular release of the medication was carried out with the assistance of confocal laser scanning microscopy (CLSM). Recently conjugation to the nanosystem, in vitro viability experiments revealed an increase in the associated DOX cytotoxicity that could not be attributable to carrier components. This indicates that the effectiveness of the DOX was increased. In light of this, it has been hypothesized that the newly created pH-triggered multifunctional Au NPs- DOX nanoparticle system could pave the way for a viable platform for the intracellular delivery of a range of anticancer medicines. In the current study, the common Au NPs synthesis techniques and their well-established uses in diverse needs, particularly in biological sensing applications.","PeriodicalId":176903,"journal":{"name":"Biomedicine and Chemical Sciences","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134085579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, Identification and Antibacterial Activity of Alkaloid Compound N-Methylcytisine from Cyanobacterium Hapalosiphon Aureus 金黄色蓝藻生物碱化合物n -甲基胞氨酸的分离鉴定及抑菌活性研究

Biomedicine and Chemical Sciences Pub Date : 2023-04-01 DOI: 10.48112/bcs.v2i2.447

Nida J. Al-Mousawi, Iqbal J. Al-Assadi, M. J. Al-Aarajy

{"title":"Isolation, Identification and Antibacterial Activity of Alkaloid Compound N-Methylcytisine from Cyanobacterium Hapalosiphon Aureus","authors":"Nida J. Al-Mousawi, Iqbal J. Al-Assadi, M. J. Al-Aarajy","doi":"10.48112/bcs.v2i2.447","DOIUrl":"https://doi.org/10.48112/bcs.v2i2.447","url":null,"abstract":"In this study, one species of algae, belongated to cyanophyta : Hapalosiphon aureus west and west 1897 were isolated, identified and purified. The extracts of algal isolates were testing to clarify their ability on growth inhibition on each of gram +ve and gram –ve bacteria E.coli & S. aureus, filamentous fungi A. fumigatus, and yeasts C.albican. The extracted alkaloid of species H.aureus showed higher inhibition activity. In our literature survey, the alkaloid compound analog N- methylcytisine was isolated for the first time from the H.aureus . This compound was characterized using Thin Layer Chromatography (TLC), Ultra Violet Spectrum (UV), Infrared Spectrum (IR), Proton Nuclear Magnetic Resonance Spectroscopy (1HNMR), Melting Point and Solubility in organic and inorganic solvents. The biological activity of this alkaloid was determined using the Minimal Inhibitory Concentration (MIC) against six bacterial isolates. The results showed inhibition in 33.3% growth of bacteria on 150 μg/ml concentration level, while the MIC against six fungi isolates showed inhibition of growth 33.3% at 50 μg/ml compared to standard antibiotic. The median lethal dose (LD50) showed no toxicity, no cytotoxicity and no effect on human red blood cells for 18 hours.","PeriodicalId":176903,"journal":{"name":"Biomedicine and Chemical Sciences","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123541471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification, Determination and Spectral Study 鉴定、测定和光谱研究

Biomedicine and Chemical Sciences Pub Date : 2023-04-01 DOI: 10.48112/bcs.v2i2.444

Wallada H. Ibrahim, H. Mahmood

{"title":"Identification, Determination and Spectral Study","authors":"Wallada H. Ibrahim, H. Mahmood","doi":"10.48112/bcs.v2i2.444","DOIUrl":"https://doi.org/10.48112/bcs.v2i2.444","url":null,"abstract":"Corona viruses resist many antiviral agents which reduces the antiviral therapies. Remdesivir is an antiviral drug efficient against single-stranded RNA viruses. Analytical determinations of this drug are very benefit in selecting the correct dose and getting real information and formulas of this compound. In this work, remdesivir has been identified at 239 nm in aqueous alkaline (1M NaOH), at 245 nm in ethanol, and at 246 nm. in methanol, it has also been followed at 239 nm using the alkaline medium and determined in injection, the linearity range was from 1to 60 µg/ml with molar absorptivity 2.036×104 l.mol-1.cm-1, and Sandell’s sensitivity index is 0.0295 µg.cm-2, LOD is 0.2613 and LOQ is 0.871 µg/mL. I. R spectrum of solid remdesivir shows band at 1639.96 cm -1 due to the carbonyl group and at 3350.79 cm-1refer to the hydroxy group adjacent to NH2 group which appears at about 3300 cm-1, the aromatic was identified at 1660-2000 cm-1 and C-O of ester at 1153cm-1. These bands in the alkaline medium were slightly shifted to be 1637 cm -1 due to carbonyl group and at 3318 cm-1refer to hydroxy group adjacent to NH2 group which appeared at about 3264 cm-1, the aromatic was identified at 2131 cm-1, and C-O of ester at 1379cm-1.","PeriodicalId":176903,"journal":{"name":"Biomedicine and Chemical Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128705389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect Of Hepatitis E Virus Open Reading Frame 3 Protein on The Expression Levels of IFN-β,IL6,TNF-α and RANTES in SW480 Colon Cancer Cells 戊型肝炎病毒开放阅读框3蛋白对SW480结肠癌细胞中IFN-β、il - 6、TNF-α和RANTES表达水平的影响

Biomedicine and Chemical Sciences Pub Date : 2023-04-01 DOI: 10.48112/bcs.v2i2.442

Rana Mohammed Abdulnabi, R. Othman, Hussein Katai Abdul Sada

{"title":"The Effect Of Hepatitis E Virus Open Reading Frame 3 Protein on The Expression Levels of IFN-β,IL6,TNF-α and RANTES in SW480 Colon Cancer Cells","authors":"Rana Mohammed Abdulnabi, R. Othman, Hussein Katai Abdul Sada","doi":"10.48112/bcs.v2i2.442","DOIUrl":"https://doi.org/10.48112/bcs.v2i2.442","url":null,"abstract":"The Hepatitis E virus (HEV) could induce chronic hepatitis and liver failure with high mortality through unclear mechanisms. The purpose of this study was to determine the effect of (recombinant fusion protein, ORF3 of genotype 1 HEV) on the expression levels of cytokine or chemokine genes in colon cancer cells (SW480) using a quantitative assay of “SYBR green\" real-time PCR. An ORF3 protein expression system (Orf3-GFP plasmid) was synthesized in the company of GenScript (USA) and used as an expression vector, SW480 cells were infected by (Orf3-GFP plasmid) or GFP- control vector. Result of this study was approved after assessing the expression level of the ORF3 gene in ORF3 expressing cells and in control groups using ORF3 specific primer to ensure that the transfection was performed successfully, The expression levels of three genes (IFN-β, IL-6, and TNF-α) were estimated in ORF3-expressing cells using specific primers for all genes. In addition, the role of HEV Orf3 in upregulating RANTES expression levels was revealed. In conclusion: In SW480 cells, this study found that the ORF3 protein of genotype 1 HEV is capable of regulating the expression of multiple immune response genes including Interferon- β (IFN-β), Interleukin-6 (IL-6) Tumor necrosis factor-α (TNF-α) and regulated on activation, normal T cell expressed and secreted (RANTES) which may enhance viral replication and pathogenicity.","PeriodicalId":176903,"journal":{"name":"Biomedicine and Chemical Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127241599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial and Antioxidant Activities of Syzygium Aromaticum Capparis Spinosa, and Some Novel Acylselenourea and Acylthourea Derivatives 香茅及一些新型酰基硒脲和酰基脲衍生物的抗菌和抗氧化活性

Biomedicine and Chemical Sciences Pub Date : 2023-04-01 DOI: 10.48112/bcs.v2i2.449

{"title":"Antibacterial and Antioxidant Activities of Syzygium Aromaticum Capparis Spinosa, and Some Novel Acylselenourea and Acylthourea Derivatives","authors":"","doi":"10.48112/bcs.v2i2.449","DOIUrl":"https://doi.org/10.48112/bcs.v2i2.449","url":null,"abstract":"This study aims to tested the antibacterial and antioxidant activities of two different plant extracts which were Syzygium aromaticum and Capparis spinosa, and five novel derivatives, i.e. [4-Nitro-N-((4-(N-(pyrimidin-2-yl) sulfamoyl) phenyl) carbamoselenoyl) Benz amide (1), 4-Methyl-N-((4-(N-(pyrimidin-2-yl) sulfamoyl) phenyl) carbamoselenoyl) Benz amide (2), 4-Methyl-N-((4-(N-(pyrimidin-2-yl) sulfamoyl phenyl) carbamothioyl) Benz amide (3), 4-Nitro-N-((4-nitrophenyl) carbamo selenoyl) Benz amide (4), N-(2,6-dioxo-1,2,3,6-tetrahydropyrimidine-1-carbonoselenoyl)-nitrobenzamide (5)] in six concentrations:1,5, 10,25, 50, and 100 ug/ml of plant extracts and in three concentrations. 500,750 ,1000ug/ml of novel synthesis compounds on gram-positive bacteria (Staphylococcus aureus) and gram-negative bacteria (Escherichia coli). The antibacterial activity was evaluated with antibiotics susceptible and resistant to microorganisms. The 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging technique was used to assess antioxidant activity. The findings showed that Syzygium aromaticum extract and synthesis compound 3 were more effective against Escherichia coli bacteria and less effective against Staphylococcus aureus bacteria, whereas Capparis spinosa extract and synthesis compounds (1, 2, 4, and 5) were more effective against Staphylococcus aureus bacteria and less effective against Escherichia coli bacteria. The oxidation of the synthesis compounds (1, 2, and 3) is effective at concentrations of 500, 750, and 1000 and ineffective at concentrations of 50, 100, and 250, while compound 4 is effective at concentrations of 100, 250, 500, 750, and 1000 and ineffective at concentrations of 50 only. Compound 5 was effective at all concentrations.","PeriodicalId":176903,"journal":{"name":"Biomedicine and Chemical Sciences","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115360853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1