Molecular Identification of Four Eruca Sativa L. Cultivars using RAPD Markers

Abstract

The current study was conducted at University of Kufa, Faculty of Science, Biology Department for molecular identification of four Eruca sativa L. cultivars (1-Turish 2-Syrian 3-Turkish 4-Eygyptian) using eleven Random Amplified Polymorphic DNA. Results indicate primers OPA-04, OPA-10, OPA-02, OPW-04, OPC-09,v and OPD-13 success in giving all cultivars a unique fingerprint .Highest number of main bands , polymorphic bands and discriminatory value were 20 , 18 and 20.22% respectively produced by OPA-02. Highest value for amplified band number and monomorphic bands were 44 and band produced by primers OPX-17 and OPD-13 respectively. Highest value for both polymorphism and Efficiency were 94.11% and 0.48 respectively produced by primer OPA-04. Genetic relationship and genetic distance among E.sativa cultivars distribution unrelated to their geographical origin. RAPD markers are efficient tool in studying E.sativa germplasm.