Journal of Venom Research最新文献_第4页

Analysis of nociceptive effects of neurotoxic phospholipase A2 from Vipera nikolskii venom in mice. 蝰蛇毒液中的神经毒性磷脂酶 A2 对小鼠痛觉效应的分析

Journal of Venom Research Pub Date : 2013-01-01 Epub Date: 2013-03-15

Igor A Dyachenko, Arkadii N Murashev, Tatyana V Andreeva, Victor I Tsetlin, Yuri N Utkin

{"title":"Analysis of nociceptive effects of neurotoxic phospholipase A2 from Vipera nikolskii venom in mice.","authors":"Igor A Dyachenko, Arkadii N Murashev, Tatyana V Andreeva, Victor I Tsetlin, Yuri N Utkin","doi":"","DOIUrl":"","url":null,"abstract":"Phospholipases A2 are represented in snake venoms by several types and possess diverse biological activities including neurotoxicity. Previously, we isolated and characterized two neurotoxic phospholipases A2 (HDP-1 and HDP-2) from the venom of Nikolski's viper (Vipera nikolskii), which were heterodimers composed of two non-covalently bound subunits. Each heterodimer consisted of an enzymatically active basic subunit and an inactive acidic subunit. In this work, we studied the in vivo biological activity of HDP-2 in mice. The acute toxicity (LD50 = 0.38 μg/gm) and maximal tolerated dose (0.1 μg/gm) were determined. In the hot plate test, HDP-2 at the maximal tolerated dose, reliably prolonged the time of the mouse staying on the plate. However, taking into account the neurotoxicity of HDP-2, we believe that this effect may be explained by a general intoxication rather than specific decrease of pain sensitivity. In this respect HDP-2 differs from other heterodimeric phospholipases A2 like crotoxin, which possess analgesic activity. This difference can be explained by the dissimilarity in the structure of the acidic subunits, suggesting an important role of this subunit in analgesic activity.","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":"4 ","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/78/24/JVR-04-001.PMC3616638.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31442854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mass landscapes of seven scorpion species: The first analyses of Australian species with 1,5-DAN matrix. 七种蝎子的质量景观：首次利用 1,5-DAN 基质对澳大利亚物种进行分析。

Journal of Venom Research Pub Date : 2012-01-01 Epub Date: 2012-10-23

Jennifer J Smith, Alun Jones, Paul F Alewood

引用次数: 0

Local and systemic biochemical alterations induced by Bothrops atrox snake venom in mice. 蛇毒诱导小鼠局部及全身生化改变。

Journal of Venom Research Pub Date : 2012-01-01 Epub Date: 2012-10-25

Carlos At de Souza, Anderson M Kayano, Sulamita S Setúbal, Adriana S Pontes, Juliana L Furtado, Fábio H Kwasniewski, Kayena D Zaqueo, Andreimar M Soares, Rodrigo G Stábeli, Juliana P Zuliani

引用次数: 0

The synergistic cytotoxic effect of cisplatin and honey bee venom on human ovarian cancer cell line A2780cp. 顺铂与蜂毒对人卵巢癌细胞系A2780cp的协同细胞毒作用。

Journal of Venom Research Pub Date : 2012-01-01 Epub Date: 2012-10-23

Masoumehzaman Alizadehnohi, Mohammad Nabiuni, Zahra Nazari, Zahra Safaeinejad, Saeed Irian

{"title":"The synergistic cytotoxic effect of cisplatin and honey bee venom on human ovarian cancer cell line A2780cp.","authors":"Masoumehzaman Alizadehnohi, Mohammad Nabiuni, Zahra Nazari, Zahra Safaeinejad, Saeed Irian","doi":"","DOIUrl":"","url":null,"abstract":"Ovarian cancer is considered to be one of the most important causes of death among women. Cisplatin is one of the oldest chemotherapeutical compounds used for treating ovarian cancer. Previous studies have shown the inhibitory effects of bee venom on certain types of cancer. The aim of the present study was to evaluate the cytotoxic effect of bee venom alone and its synergistic cytological effects in combination with cisplatin on ovarian cancerous cisplatin resistant A2780cp cells. To investigate the cytotoxic effect of bee venom on A2780cp cells and its synergetic effect with cisplatin, MTT assay, morphological examination, DNA fragmentation assay, flowcytometric and immunocytochemical analysis were performed. MTT assay revealed that 8µg/ml bee venom, 25mg/ml cisplatin and 4µg/ml bee venom/10mg/ml cisplatin cause an approximately 50% A2780cp cell death after 24hr. Morphological and biochemical analysis indicated an apoptotic type of cell death induced by bee venom and cisplatin, separately and in combination. Immunocytochemistry demonstrated a reduction in the levels of the Bcl2 protein. Overall, our findings suggest that components of bee venom may exert an anti-tumor effect on human ovarian cancer and that has the potential for enhancing the cytotoxic effect of the antitumor agent cisplatin.","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":" ","pages":"22-7"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ad/e9/JVR-03-022.PMC3522379.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40221606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoparticle-conjugated animal venom-toxins and their possible therapeutic potential. 纳米颗粒结合的动物毒液毒素及其可能的治疗潜力。

Journal of Venom Research Pub Date : 2012-01-01 Epub Date: 2012-10-23

Archita Biswas, Aparna Gomes, Jayeeta Sengupta, Poulami Datta, Santiswarup Singha, Anjan Kr Dasgupta, Antony Gomes

{"title":"Nanoparticle-conjugated animal venom-toxins and their possible therapeutic potential.","authors":"Archita Biswas, Aparna Gomes, Jayeeta Sengupta, Poulami Datta, Santiswarup Singha, Anjan Kr Dasgupta, Antony Gomes","doi":"","DOIUrl":"","url":null,"abstract":"Nano-medical approaches to develop drugs have attracted much attention in different arenas to design nanoparticle conjugates for better efficacy of the potential bio-molecules. A group of promising candidates of this category would be venom-toxins of animal origin of potential medicinal value. Traditional systems of medicine as well as folklores mention the use of venom-toxins for the treatment of various diseases. Research has led to scientific validation of medicinal applications of venoms-toxins and many active constituents derived from venoms-toxins are already in clinical use or under clinical trial. Nanomedicine is an emerging field of medicine where nanotechnology is used to develop molecules of nano-scale dimension, so that these molecules can be taken up by the cells more easily and have better efficacy, as compared to large molecules that may tend to get eliminated. This review will focus on some of the potential venoms and toxins along with nanoparticle conjugated venom-toxins of snakes, amphibians, scorpions and bees, etc., for possible therapeutic clues against emerging diseases.","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":"3 ","pages":"15-21"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2d/68/JVR-03-015.PMC3518302.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31121024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The pharmacokinetics of Hemiscorpius lepturus scorpion venom and Razi antivenom following intramuscular administration in rat. 半角蝎毒和拉兹抗蛇毒在大鼠肌肉注射后的药动学研究。

Journal of Venom Research Pub Date : 2012-01-01 Epub Date: 2012-04-24

Amir Jalali, Nazanin Bavarsad-Omidian, Mohammad Babaei, Hossein Najafzadeh, Saeed Rezaei

{"title":"The pharmacokinetics of Hemiscorpius lepturus scorpion venom and Razi antivenom following intramuscular administration in rat.","authors":"Amir Jalali, Nazanin Bavarsad-Omidian, Mohammad Babaei, Hossein Najafzadeh, Saeed Rezaei","doi":"","DOIUrl":"","url":null,"abstract":"Hemiscorpius lepturus (H. lepturus) is one of the most dangerous scorpions in Iran. Intramuscular administration (IM) of available Razi antivenom to H. lepturus venom is used by many of Iranian clinicians. The purpose of the current study was to investigate the efficiency of IM route for treatment of envenomed patients by H. lepturus. We compared the pharmacokinetics parameters of venom and antivenom via subcutaneous (SC) and IM administration, respectively. The blood samples were taken at various predetermined time intervals, i.e., 10, 40, 60, 180, 210, 360 and 400min following 5μg (131)I-labeled venom and 5, 10, 40, 120 and 360min following 0.2ml of (131)I-labeled antivenom administration. The radio-iodination was carried out using the chloramin-T method. The results showed that pharmacokinetic parameters of the venom were T(elimination half-life) = 103.25min; Vd/F (apparent volume of distribution) = 14.9ml/kg; Cl/F (total blood clearance) = 0.04ml/kg/min; mean resident residual time (MRT) = 244.3min, and for the antivenom T(1/2) = 628.59min, Vd = 666.66ml/kg, Cl = 0.13ml/kg/min and MRT = 1292min. A comparison of pharmacokinetic profiles indicated that the intramuscular administration was helpful in the referral less than 2hr to clinical centers but not those exceeding 3hr. Overall, the data showed that immunotherapy against H. lepturus stings was likely to be more effective through intravenous administration.","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":"3 ","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1b/10/JVR-03-001.PMC3399132.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30785676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suramin inhibits the early effects of PLA(2) neurotoxins at mouse neuromuscular junctions: A twitch tension study. 苏拉明抑制PLA(2)神经毒素在小鼠神经肌肉连接处的早期作用:一项抽搐张力研究。

Journal of Venom Research Pub Date : 2011-01-02

Behrooz Fathi, Alan L Harvey, Edward G Rowan

{"title":"Suramin inhibits the early effects of PLA(2) neurotoxins at mouse neuromuscular junctions: A twitch tension study.","authors":"Behrooz Fathi, Alan L Harvey, Edward G Rowan","doi":"","DOIUrl":"","url":null,"abstract":"Several phospholipase A(2) (PLA(2)) neurotoxins from snake venoms can affect acetylcholine release at the neuromuscular junction. In isolated nerve-muscle preparations three distinct phases have been described for this phenomenon: An initial transient decrease in twitch tension; a second facilitatory phase during which twitch height is greater than control twitch height; and the last phase which causes a reduction in twitch height that finally results in paralysis. Suramin has been reported to inhibit the toxic effects of β-bungarotoxin and another PLA(2) neurotoxin, crotoxin in vitro and in vivo. We have further examined the effects of suramin on the three phases of the effects of the presynaptic PLA(2) neurotoxins β-bungarotoxin, taipoxin and ammodytoxin on mouse phrenic nerve-hemidiaphragm preparations. When preparations were pre-treated with suramin (0.3mM), the early biphasic effects (depression followed by facilitation) were abolished, and the time taken for final blockade induced by β-bungarotoxin, taipoxin and ammodytoxin A was significantly prolonged. In contrast, suramin did not significantly affect the facilitation induced by the potassium channel blocking toxin dendrotoxin I when applied under the same conditions. In addition, application of 0.3mM suramin did not prevent the facilitatory actions of 3,4-diaminopyridine (3,4-DAP) and tetraethylammonium chloride (TEA). Overall, the mechanism whereby suramin reduces the effects of PLA(2) neurotoxins remains elusive. Since suramin reduces both enzyme-dependent and enzyme-independent effects of the toxins, suramin is not acting as a simple enzyme inhibitor. Furthermore, the observation that suramin does not affect actions of standard K(+) channel blockers suggests that suramin does not stabilise nerve terminals.","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":" ","pages":"6-10"},"PeriodicalIF":0.0,"publicationDate":"2011-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a2/d8/JVR-02-006.PMC3086180.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29859704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TTX, cations and spider venom modify avian muscle tone in vitro. TTX、阳离子和蜘蛛毒液对禽类肌张力的体外调节作用。

Journal of Venom Research Pub Date : 2011-01-02

Volker Herzig, Wayne C Hodgson, Edward G Rowan

引用次数: 0

The neuromuscular activity of Micrurus pyrrhocryptus venom and its neutralization by commercial and specific coral snake antivenoms. 红嘴蛇毒液的神经肌肉活性及其在商用和专用珊瑚蛇毒血清中的中和作用。

Journal of Venom Research Pub Date : 2011-01-01 Epub Date: 2011-06-24

Thiago Magalhães Camargo, Adolfo Rafael de Roodt, Maria Alice da Cruz-Höfling, Léa Rodrigues-Simioni

{"title":"The neuromuscular activity of Micrurus pyrrhocryptus venom and its neutralization by commercial and specific coral snake antivenoms.","authors":"Thiago Magalhães Camargo, Adolfo Rafael de Roodt, Maria Alice da Cruz-Höfling, Léa Rodrigues-Simioni","doi":"","DOIUrl":"","url":null,"abstract":"The neuromuscular activity ofMicrurus pyrrochryptus venom was studied in chick biventer cervicis (BC) and mouse phrenic nerve-diaphragm (PND) preparations. The venom (0.5-50μg/ml) caused irreversible, time- and concentration-dependent blockade, with BC being more sensitive than PND (50% blockade with 10μg/ml in 22±;3min and 62±4min, respectively; mean±SEM, n=6; p<0.05). In BC preparations, venom (0.5μg/ml) progressively abolished ACh-induced contractures, whereas contractures to exogenous KCl and muscle twitches in curarized preparations were unaffected. The venom neither altered creatine kinase release (venom: 25.8±1.75IU/l vs control: 24.3±2.2IU/l, n=6, after 120min), nor it caused significant muscle damage (50μg of venom/ml vs control: 3.5±0.8% vs 1.1±0.7% for PND; 4.3±1.5% vs 1.2±0.5% for BC, n=5). The venom had low PLA(2) activity. Neurotoxicity was effectively neutralized by commercial Micrurus antivenom and specific antivenom. These findings indicate that M. pyrrhocryptus venom acts postsynaptically on nicotinic receptors, with no significant myotoxicity.","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":" ","pages":"24-31"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a0/0f/JVR-02-024.PMC3132105.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30090880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological characterization of Bothrops marajoensis snake venom. 马拉霍Bothrops蛇毒的生物学特性。

Journal of Venom Research Pub Date : 2011-01-01 Epub Date: 2011-10-19

Walter Lg Cavalcante, Saraguaci Hernandez-Oliveira, Charlene Galbiatti, Priscila Randazzo-Moura, Thalita Rocha, Luis Ponce-Soto, Sérgio Marangoni, Maeli Dal Pai-Silva, Márcia Gallacci, Maria A da Cruz-Höfling, Léa Rodrigues-Simioni

{"title":"Biological characterization of Bothrops marajoensis snake venom.","authors":"Walter Lg Cavalcante, Saraguaci Hernandez-Oliveira, Charlene Galbiatti, Priscila Randazzo-Moura, Thalita Rocha, Luis Ponce-Soto, Sérgio Marangoni, Maeli Dal Pai-Silva, Márcia Gallacci, Maria A da Cruz-Höfling, Léa Rodrigues-Simioni","doi":"","DOIUrl":"","url":null,"abstract":"This study describes the effects of Bothrops marajoensis venom (Marajó lancehead) on isolated neuromuscular preparations of chick biventer cervicis (CBC) and mouse phrenic nerve-diaphragm (PND). At low concentrations (1µg/ml for CBC and 5µg/ml for PND), the venom exhibited a neuromuscular blocking without any damaging effect on the muscle integrity. At higher concentration (20μg/ml for PND), together with the neuromuscular blockade, there was a moderate myonecrosis. The results show differences between mammalian and avian preparations in response to venom concentration; the avian preparation was more sensitive to venom neurotoxic effect than the mammalian preparation. The possible presynaptic mechanism underlying the neuromuscular blocking effect was reinforced by the observed increase in MEPPs at the same time (at 15min) when the facilitation of twitch tension occurred. These results indicate that the B. marajoensis venom produced neuromuscular blockade, which appeared to be presynaptic at low concentrations with a postsynaptic component at high concentrations, leading to muscle oedema. These observations demand the fractionation of the crude venom and characterization of its active components for a better understanding of its biological dynamics.","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":" ","pages":"37-41"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4c/eb/JVR-02-037.PMC3211074.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30260835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0