Journal of Venom Research最新文献_第3页

Recombinant disintegrin (r-Cam-dis) from Crotalus adamanteus inhibits adhesion of human pancreatic cancer cell lines to laminin-1 and vitronectin. 重组金刚蟾崩解素(r-Cam-dis)抑制人胰腺癌细胞系对层粘连蛋白-1和玻璃体粘连蛋白的粘附。

Journal of Venom Research Pub Date : 2015-04-26 eCollection Date: 2015-01-01

Montamas Suntravat, Henriquez S Barret, Cameron A Jurica, Sara E Lucena, John C Perez, Elda E Sánchez

{"title":"Recombinant disintegrin (r-Cam-dis) from Crotalus adamanteus inhibits adhesion of human pancreatic cancer cell lines to laminin-1 and vitronectin.","authors":"Montamas Suntravat, Henriquez S Barret, Cameron A Jurica, Sara E Lucena, John C Perez, Elda E Sánchez","doi":"","DOIUrl":"","url":null,"abstract":"Pancreatic cancer is a malignant cancer common worldwide having poor prognosis, even when diagnosed at its early stage. Cell adhesion plays a critical role in cancer invasion and metastasis. Integrins are major mediators of cell adhesion and play an important role in invasion and metastatic growth of human pancreatic cancer cells. Snake disintegrins are the most potent ligands of several integrins and have potential therapeutic applications for cancers. We have previously cloned and expressed a new recombinant RGD-disintegrin from Crotalus adamanteus (r-Cam-dis). This recently published r-Cam-dis has an extra nine amino acids derived from the vector (SPGARGSEF) at the N-terminus end and has strong anti-platelet activity. However, this r-Cam-dis contains the contamination of the cleavage of the N-terminal end of the pET-43.1a cloning vector. In this study, we have cloned r-Cam-dis in a different cloning vector (pGEX-4T-1) showing five different amino acids (GSPEF) at the N-terminal part. This new r-Cam-dis was expressed and tested for inhibition of platelet aggregation, specific binding activity with seven different integrins, and inhibition of adhesion of three different pancreatic cancer cell lines on laminin-1 and vitronectin. The r-Cam-dis showed potent binding to αvβ3 integrin, but was moderate to weak with αvβ5, αvβ6, α2β1, and α6β1. Interestingly, the inhibition of r-Cam-dis on pancreatic cancer cell lines adhesion to laminin-1 was more effective than that to vitronectin. Based on our binding results to integrin receptors and previous adhesion studies using function-blocking monoclonal antibodies, it is suggested that r-Cam-dis could be inhibiting adhesion of pancreatic cancer cell lines through integrins α2β1, α6β1, αvβ5, and αvβ6. ","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":"6 ","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2015-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d0/e0/JVR-06-01.PMC4440708.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33361743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plectreurys tristis venome: A proteomic and transcriptomic analysis. 斑马病:蛋白质组学和转录组学分析。

Journal of Venom Research Pub Date : 2014-09-20 eCollection Date: 2014-01-01

Pamela A Zobel-Thropp, Emily Z Thomas, Cynthia L David, Linda A Breci, Greta J Binford

{"title":"Plectreurys tristis venome: A proteomic and transcriptomic analysis.","authors":"Pamela A Zobel-Thropp, Emily Z Thomas, Cynthia L David, Linda A Breci, Greta J Binford","doi":"","DOIUrl":"","url":null,"abstract":"Spider venoms are complex cocktails rich in peptides, proteins and organic molecules that collectively act to immobilize prey. Venoms of the primitive hunting spider, Plectreurys tristis, have numerous neurotoxic peptides called \"plectoxins\" (PLTX), a unique acylpolyamine called bis(agmatine)oxalamide, and larger unidentified protein components. These spiders also have unconventional multi-lobed venom glands. Inspired by these unusual characteristics and their phylogenetic position as Haplogynes, we have partially characterized the venome of P. tristis using combined transcriptomic and proteomic methods. With these analyses we found known venom neurotoxins U1-PLTX-Pt1a, U3-PLTX-Pt1a, and we discovered new groups of potential neurotoxins, expanding the U1- and ω-PLTX families and adding U4-through U9-PLTX as six new groups. The venom also contains proteins that are homologs of astacin metalloproteases that, combined with venom peptides, make up 94% of components detected in crude venom, while the remaining 6% is a single undescribed protein with unknown function. Other proteins detected in the transcriptome were found to be members of conserved gene families and make up 20% of the transcripts. These include cDNA sequences that match venom proteins from Mesobuthus and Hottentotta scorpions, Loxosceles and Dysdera spiders, and also salivary and secreted peptide sequences from Ixodes, Amblyomma and Rhipicephalus ticks. Finally, we show that crude venom has neurotoxic effects and an effective paralytic dose on crickets of 3.3µg/gm. ","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":"5 ","pages":"33-47"},"PeriodicalIF":0.0,"publicationDate":"2014-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d3/f8/JVR-05-33.PMC4231235.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32817428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hemolytic venoms from marine cnidarian jellyfish - an overview. 海洋刺胞水母的溶血毒液综述。

Journal of Venom Research Pub Date : 2014-07-23 eCollection Date: 2014-01-01

Gian Luigi Mariottini

引用次数: 0

Peptides with in vitro anti-tumor activity from the venom of the Eastern green mamba, Dendroaspis angusticeps (Elapidae). 从东部绿曼巴，Dendroaspis angusticeps毒液中提取具有体外抗肿瘤活性的肽。

Journal of Venom Research Pub Date : 2014-06-19 eCollection Date: 2014-01-01

J Michael Conlon, Manju Prajeep, Milena Mechkarska, Kholoud Arafat, Samir Attoub, Abdu Adem, Davinia Pla, Juan J Calvete

{"title":"Peptides with in vitro anti-tumor activity from the venom of the Eastern green mamba, Dendroaspis angusticeps (Elapidae).","authors":"J Michael Conlon, Manju Prajeep, Milena Mechkarska, Kholoud Arafat, Samir Attoub, Abdu Adem, Davinia Pla, Juan J Calvete","doi":"","DOIUrl":"","url":null,"abstract":"Two structurally related (48.6% amino acid sequence identity) peptides with cytotoxic activity against human non-small cell lung adenocarcinoma A549 cells were purified from the venom of the Eastern green mamba Dendroaspis angusticeps using reversed phase HPLC. The peptides were identified as members of the three-finger superfamily of snake toxins by mass fingerprinting of tryptic digests. The more potent peptide (LC50 against A549 cells = 56±4µg/ml) was identical to the previously described toxin C13S1C1 and the less active peptide (LC50 against A549 cells = 106±5µg/ml) was identical to toxin F-VIII. Toxin C13S1C1 was also cytotoxic against breast adenocarcinoma MDA-MB-231 cells (LC50 = 62±2µg/ml) and colorectal adenocarcinoma HT-29 cells (LC50 = 110±4µg/ml). Although the peptide was appreciably less hemolytic activity against human erythrocytes (LC50 >600µg/ml), it was cytotoxic to human umbilical vein endothelial HUVEC cells (57±3µg/ml) indicating no differential activity against cell lines derived from neoplastic tissues. Toxin F-VIII was not cytotoxic to MDA-MB-231, HT-29 cells, and HUVEC cells at concentrations up to 300µg/ml and was not hemolytic at concentrations up to 1mg/ml. Neither peptide inhibited growth of reference strains of Escherichia coli or Staphylococcus aureus (MIC values >200μg/ml). ","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":"5 ","pages":"16-21"},"PeriodicalIF":0.0,"publicationDate":"2014-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3d/98/JVR-05-16.PMC4102125.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32513502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuromuscular activity of Bothrops fonsecai snake venom in vertebrate preparations. 脊椎动物制剂中蛇毒的神经肌肉活性。

Journal of Venom Research Pub Date : 2014-06-18 eCollection Date: 2014-01-01

Carla T Fernandes, Vânia Ma Giaretta, Luiz S Prudêncio, Edvana O Toledo, Igor Rf da Silva, Rita Co Collaço, Ana M Barbosa, Stephen Hyslop, Léa Rodrigues-Simioni, José C Cogo

{"title":"Neuromuscular activity of Bothrops fonsecai snake venom in vertebrate preparations.","authors":"Carla T Fernandes, Vânia Ma Giaretta, Luiz S Prudêncio, Edvana O Toledo, Igor Rf da Silva, Rita Co Collaço, Ana M Barbosa, Stephen Hyslop, Léa Rodrigues-Simioni, José C Cogo","doi":"","DOIUrl":"","url":null,"abstract":"The neuromuscular activity of venom from Bothrops fonsecai, a lancehead endemic to southeastern Brazil, was investigated. Chick biventer cervicis (CBC) and mouse phrenic nerve-diaphragm (PND) preparations were used for myographic recordings and mouse diaphragm muscle was used for membrane resting potential (RP) and miniature end-plate potential (MEPP) recordings. Creatine kinase release and muscle damage were also assessed. In CBC, venom (40, 80 and 160μg/ml) produced concentration- and time-dependent neuromuscular blockade (50% blockade in 85±9 min and 73±8 min with 80 and 160μg/ml, respectively) and attenuated the contractures to 110μM ACh (78-100% inhibition) and 40mM KCl (45-90% inhibition). The venom-induced decrease in twitch-tension in curarized, directly-stimulated preparations was similar to that in indirectly stimulated preparations. Venom (100 and 200μg/ml) also caused blockade in PND preparations (50% blockade in 94±13 min and 49±8 min with 100 and 200μg/ml, respectively) but did not alter the RP or MEPP amplitude. In CBC, venom caused creatine kinase release and myonecrosis. The venom-induced decrease in twitch-tension and in the contractures to ACh and K(+) were abolished by preincubating venom with commercial antivenom. These findings indicate that Bothrops fonsecai venom interferes with neuromuscular transmission essentially through postsynaptic muscle damage that affects responses to ACh and KCl. These actions are effectively prevented by commercial antivenom. ","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":"5 ","pages":"6-15"},"PeriodicalIF":0.0,"publicationDate":"2014-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a4/c0/JVR-05-06.PMC4095944.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32509421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capillary damage in the area postrema by venom of the northern black-tailed rattlesnake (Crotalus molossus molossus). 北方黑尾响尾蛇(Crotalus molossus molossus)毒液对术后毛细血管的损伤。

Journal of Venom Research Pub Date : 2014-04-04 eCollection Date: 2014-01-01

David Meléndez-Martínez, Eduardo Macias-Rodríguez, Alejandra Vargas-Caraveo, Alejandro Martínez-Martínez, Ana Gatica-Colima, Luis Fernando Plenge-Tellechea

{"title":"Capillary damage in the area postrema by venom of the northern black-tailed rattlesnake (Crotalus molossus molossus).","authors":"David Meléndez-Martínez, Eduardo Macias-Rodríguez, Alejandra Vargas-Caraveo, Alejandro Martínez-Martínez, Ana Gatica-Colima, Luis Fernando Plenge-Tellechea","doi":"","DOIUrl":"","url":null,"abstract":"The Northern black-tailed rattlesnake (Crotalus molossus molossus) venom is mainly hemotoxic, hemorrhagic, and neurotoxic. Its effects in the central nervous system are unknown and only poorly described for all Viperidae species in general. This is why we are interested in describe the damage induced by C. m. molossus venom in rat brain, particularly in the area postrema capillaries. Four C. m. molossus venom doses were tested (0.02, 0.05, 0.10 and 0.20mg/kg) injected intramuscularly at the lower limb, incubated by 24 hours and the brains were harvested. Area postrema coronal sections were stained with Haematoxylin and Eosin, and examined to observe the venom effect in quantity of capillaries and porphology. Starting from the 0.10mg/kg treatment we observed lysed extravasated erythrocytes and also capillary breakdown, as a consequence of hemorrhages appearance. The number of capillaries decreased significantly in response to the venom dose increment. Hemorrhages could be caused by the metalloproteinase activity on the basal membrane and the apoptosis generated by L-amino acid oxidases. Hemolysis could be caused by phospholipase A2 hemotoxic effect. We conclude that C. m. molossus crude venom produces hemolysis, capillary breakdown, hemorrhages, and the reduction in number of capillaries in the area postrema. ","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":"5 ","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2014-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/08/72/JVR-05-01.PMC4102126.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32513501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biochemical and biological characterization of Naja kaouthia venom from North-East India and its neutralization by polyvalent antivenom. 产自印度东北部的蛇舌蛇毒的生化生物学特性及其多价抗蛇毒血清的中和作用。

Journal of Venom Research Pub Date : 2013-11-06 eCollection Date: 2013-01-01

Diganta Das, Nanjaraj Urs, Vilas Hiremath, Bannikuppe Sannanaik Vishwanath, Robin Doley

{"title":"Biochemical and biological characterization of Naja kaouthia venom from North-East India and its neutralization by polyvalent antivenom.","authors":"Diganta Das, Nanjaraj Urs, Vilas Hiremath, Bannikuppe Sannanaik Vishwanath, Robin Doley","doi":"","DOIUrl":"","url":null,"abstract":"This study describes biochemical and biological properties of Naja kaouthia (Indian monocled cobra) venom of North-East India. The LD50 of the crude venom was found to be 0.148mg/kg and neurotoxicitic symptoms like paralysis of lower limbs and heavy difficulty in breathing at sub-lethal dose in mice was observed. The venom exhibited PLA2, indirect hemolytic and myotoxic activities but showed weak proteolytic and low direct hemolytic activities. It did not exhibit any hemorrhage when injected intradermally to mice. Anticoagulant activity was prominent when recalcification, prothrombin and activated partial thrombinplastin time were tested on platelet poor plasma. Rotem analysis of whole citrated blood in presence of venom showed delay in coagulation time and clot formation time. Fibrinogen of whole citrated blood was depleted by venom when analyzed in Sonoclot. Crude venom at 10µg and after 16hr of incubation was found to degrade α chain of fibrinogen. Neutralization study showed that Indian polyvalent antivenom could neutralize some of the biochemical and biological activities as well as its fibrinogenolytic activity. ","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":"4 ","pages":"31-8"},"PeriodicalIF":0.0,"publicationDate":"2013-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1f/53/JVR-04-031.PMC3861878.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31965091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of selected Australian snake venoms on tumour-associated microvascular endothelial cells (TAMECs) in vitro. 澳大利亚蛇毒对体外肿瘤相关微血管内皮细胞(TAMECs)的影响

Journal of Venom Research Pub Date : 2013-10-19 eCollection Date: 2013-01-01

Emma Bateman, Michael Venning, Peter Mirtschin, Anthony Woods

{"title":"The effects of selected Australian snake venoms on tumour-associated microvascular endothelial cells (TAMECs) in vitro.","authors":"Emma Bateman, Michael Venning, Peter Mirtschin, Anthony Woods","doi":"","DOIUrl":"","url":null,"abstract":"The effects of various viperid and elapid venoms on the cellular biology of tumour-associated microvascular endothelial cells (TAMECs) were determined in the current study using cells isolated from a rat mammary adenocarcinoma. Previous studies to determine the effects of snake venoms on endothelial cells in vitro have in the main been performed on either human umbilical vein endothelial cells (HUVECs), bovine aortic endothelial cells (BAECs) or endothelial cell lines. These cell populations are accessible and easy to maintain in culture, however, it is well established that endothelial cells display vast heterogeneity depending upon the local microenvironment of the tissue from which they are isolated. Vascular targeting agents have been isolated from a variety of snake venoms, particularly from snakes of the Viperidae family, but it is yet to be established to what extent the venoms from Australian elapids possess similar vascular targeting properties. The present study used endothelial cells (ECs) isolated from the microvasculature of a rat mammary adenocarcinoma to determine the effects of a panel of snake venoms, including viperid venoms with known apoptotic activity and elapid venoms (both exotic and indigenous to Australia), on endothelial morphology and viability, paying specific attention to apoptotic responses. Three of the five Australian snake venoms investigated in this study elicited significant apoptotic responses in ECs which were in many ways similar to responses elicited by the selected viperid venoms. This suggests that these Australian elapids may possess vascular targeting components similar to those found within viperid venoms. ","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":"4 ","pages":"21-30"},"PeriodicalIF":0.0,"publicationDate":"2013-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/94/97/JVR-04-021.PMC3812074.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31833320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of intraspecific variation in venoms of Acanthophis antarcticus death adders from South Australia. 南澳大利亚南极棘蛛(Acanthophis antarcticus death蝰蛇)毒液种内变异分析。

Journal of Venom Research Pub Date : 2013-08-25 eCollection Date: 2013-01-01

Volker Herzig, Maxie Kohler, Kai F Grund, Shane Reeve, A Ian Smith, Wayne C Hodgson

引用次数: 0

In vitro anticancer effect of venom from Cuban scorpion Rhopalurus junceus against a panel of human cancer cell lines. 古巴蝎(Rhopalurus junceus)毒液对一组人类癌细胞的体外抗癌作用。

Journal of Venom Research Pub Date : 2013-06-12 eCollection Date: 2013-01-01

Alexis Díaz-García, Luis Morier-Díaz, Yahima Frión-Herrera, Hermis Rodríguez-Sánchez, Yamira Caballero-Lorenzo, Dianeya Mendoza-Llanes, Yanelis Riquenes-Garlobo, José A Fraga-Castro

{"title":"In vitro anticancer effect of venom from Cuban scorpion Rhopalurus junceus against a panel of human cancer cell lines.","authors":"Alexis Díaz-García, Luis Morier-Díaz, Yahima Frión-Herrera, Hermis Rodríguez-Sánchez, Yamira Caballero-Lorenzo, Dianeya Mendoza-Llanes, Yanelis Riquenes-Garlobo, José A Fraga-Castro","doi":"","DOIUrl":"","url":null,"abstract":"In Cuba the endemic species of scorpion Rhopalurus junceus has been used in traditional medicine for cancer treatment. However, there is little scientific evidence about its potential in cancer therapy. The effect of a range of scorpion venom concentrations (0.1, 0.25, 0.5, 0.75 and 1mg/ml) against a panel of human tumor cell lines from epithelial (Hela, SiHa, Hep-2, NCI-H292, A549, MDA-MB-231, MDA-MB-468, HT-29), hematopoietic origins (U937, K562, Raji) and normal cells (MRC-5, MDCK, Vero) was determined by the MTT assay. Additionally, the effect of venom on tumor cell death was assayed by Fluorescence microscopy, RT-PCR and western blot. Only the epithelial cancer cells showed significant cell viability reduction, with medium cytotoxic concentration (IC50) ranging from 0.6-1mg/ml, in a concentration-dependent manner. There was no effect on either normal or hematopoietic tumor cells. Scorpion venom demonstrated to induce apoptosis in less sensitive tumor cells (Hela) as evidenced by chromatin condensation, over expression of p53 and bax mRNA, down expression of bcl-2 mRNA and increase of activated caspases 3, 8, 9. In most sensitive tumor cells (A549), scorpion venom induced necrosis evidenced by acridine orange/ethidium bromide fluorescent dyes and down-expression of apoptosis-related genes. We concluded the scorpion venom from R. junceus possessed a selective and differential toxicity against epithelial cancer cells. This is the first report related to biological effect of R. junceus venom against a panel of tumor cells lines. All these results make R. junceus venom as a promise natural product for cancer treatment. ","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":"4 ","pages":"5-12"},"PeriodicalIF":0.0,"publicationDate":"2013-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/59/8c/JVR-04-005.PMC3717326.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31658322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0