Journal of Venom Research最新文献_第5页

Characterization of crude Echis carinatus venom-induced cytotoxicity in HEK 293T cells. 粗鲫鱼毒液诱导HEK 293T细胞毒性的研究。

Journal of Venom Research Pub Date : 2011-01-01 Epub Date: 2011-12-28

Rebecca D Pierce, Ethan S Kim, Lance W Girton, Jonathan L McMurry, Joshua W Francis, Eric A Albrecht

引用次数: 0

Purification of a phospholipase A(2) from Daboia russelii siamensis venom with anticancer effects. 从具有抗癌作用的暹罗豚毒液中纯化出一种磷脂酶 A(2)

Journal of Venom Research Pub Date : 2011-01-01 Epub Date: 2011-10-22

Suchitra Khunsap, Narumol Pakmanee, Orawan Khow, Lawan Chanhome, Visith Sitprija, Montamas Suntravat, Sara E Lucena, John C Perez, Elda E Sánchez

引用次数: 0

Presynaptic effect of a methanolic extract of toad (Rhinella schneideri) poison in avian neuromuscular preparation. 蟾蜍毒甲醇提取物在鸟类神经肌肉制剂中的突触前作用。

Journal of Venom Research Pub Date : 2011-01-01 Epub Date: 2011-09-22

Sandro Rostelato-Ferreira, Cháriston A Dal Belo, Maria Alice da Cruz-Höfling, Stephen Hyslop, Léa Rodrigues-Simioni

引用次数: 0

The in vitro toxicity of venoms from South Asian hump-nosed pit vipers (Viperidae: Hypnale). 南亚驼鼻蝮蛇（蝰科：Hypnale）毒液的体外毒性。

Journal of Venom Research Pub Date : 2011-01-01 Epub Date: 2011-06-08

Kalana Maduwage, Wayne C Hodgson, Nicki Konstantakopoulos, Margaret A O'Leary, Indika Gawarammana, Geoffrey K Isbister

{"title":"The in vitro toxicity of venoms from South Asian hump-nosed pit vipers (Viperidae: Hypnale).","authors":"Kalana Maduwage, Wayne C Hodgson, Nicki Konstantakopoulos, Margaret A O'Leary, Indika Gawarammana, Geoffrey K Isbister","doi":"","DOIUrl":"","url":null,"abstract":"Hump-nosed pit vipers (Genus Hypnale) are venomous snakes from South India and Sri Lanka. Envenoming by Hypnale species may cause significant morbidity and is characterized by local envenoming and less commonly coagulopathy and acute renal failure. Currently there are three nominal species of this genus: H. hypnale, H. zara and H. nepa. This study investigates the biochemical and pharmacological properties of the venoms from the three Hypnale species in Sri Lanka. The three Hypnale venoms had similar chromatographic profiles using reverse phase high performance liquid chromatography and fractions with procoagulant activity were identified. Hypnale venoms had potent cytotoxicity in cultured rat aorta smooth muscle cells with similar IC(50) values. The venoms had weak neurotoxic and myotoxic activity in the isolated chick biventer muscle preparation. They had mild procoagulant activity with close MCC(5) values and also phospholipase activity. Locally available polyvalent antivenom did not neutralise any venom effects. The study demonstrates that the three Hypnale venoms are similar and cytotoxicity appears to be the most potent effect, although they have mild procoagulant activity. These findings are consistent with clinical reports.","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":" ","pages":"17-23"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2f/5c/JVR-02-017.PMC3114463.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29940674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypericum brasiliense plant extract neutralizes some biological effects of Bothrops jararaca snake venom. 巴西金丝桃植物提取物可中和刺蒺藜蛇毒的部分生物效应。

Journal of Venom Research Pub Date : 2011-01-01 Epub Date: 2011-05-25

Mariane Assafim, Eduardo Coriolano de Coriolano, Sérgio Eufrázio Benedito, Caio Pinho Fernandes, Jonathas Felipe Revoredo Lobo, Eladio Florez Sanchez, Leandro Machado Rocha, André Lopes Fuly

{"title":"Hypericum brasiliense plant extract neutralizes some biological effects of Bothrops jararaca snake venom.","authors":"Mariane Assafim, Eduardo Coriolano de Coriolano, Sérgio Eufrázio Benedito, Caio Pinho Fernandes, Jonathas Felipe Revoredo Lobo, Eladio Florez Sanchez, Leandro Machado Rocha, André Lopes Fuly","doi":"","DOIUrl":"","url":null,"abstract":"Alternative treatments for snake bite are currently being extensively studied, and plant metabolites are considered good candidates for such purpose. Here, the ability of a crude ethanolic extract of Hypericum brasiliense plant in neutralizing Bothrops jararaca snake venom was investigated by in vitro (coagulation, hemolysis or proteolysis) and in vivo (hemorrhage, lethality and edema) biological assays. We describe for the first time the ability of H. brasiliense extracts to inhibit some pharmacological effects of a Brazilian snake venom. Inhibitory assays were performed by incubating B. jararaca venom with H. brasiliense extracts for 30min at room temperature before the assays were performed. The results showed that H. brasiliense extracts impaired lethality, edema, hemorrhage, hemolysis, proteolysis as well as fibrinogen or plasma clotting induced by B. jararaca venom. This indicates that H. brasiliense extracts can provide promising agents to treat B. jararaca envenomation.","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":" ","pages":"11-6"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3108466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29921869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibition of Bothrops jararacussu venom activities by Plathymenia reticulata Benth extracts. 网状白兜草提取物对鼠足虱毒液活性的抑制作用。

Journal of Venom Research Pub Date : 2011-01-01 Epub Date: 2011-12-28

Farrapo Nicole M, Silva Gleidy Aa, Costa Karine N, Silva Magali G, Cogo José C, Belo Cháriston A Dal, Santos Márcio G Dos, Groppo Francisco C, Oshima-Franco Yoko

{"title":"Inhibition of Bothrops jararacussu venom activities by Plathymenia reticulata Benth extracts.","authors":"Farrapo Nicole M, Silva Gleidy Aa, Costa Karine N, Silva Magali G, Cogo José C, Belo Cháriston A Dal, Santos Márcio G Dos, Groppo Francisco C, Oshima-Franco Yoko","doi":"","DOIUrl":"","url":null,"abstract":"Hexane (HEX), dichloromethane (DM), ethyl acetate (EA) and methanol (M) extracts (0.1, 0.2 and 0.4mg/ml) were obtained via Soxhlet from Plathymenia reticulata barks (Pr). These extracts were evaluated against the myotoxicity (58%) and the irreversible in vitro neuromuscular blockade of Bothrops jararacussu (Bjssu) venom (40μg/ml) in a mouse phrenic-nerve diaphragm preparation, by using light-microscopy and conventional myographic techniques. Thin layer chromatography (TLC) was used to access the basic composition of extracts. The efficacy of the extracts was analyzed by Student's t-test or repeated measures ANOVA. The significance level was set at 5%. The Pr extracts showed a higher polyphenols content (3.75%), from which tannins take part, around 20 times more than flavonoids content (0.16%). Qualitatively, via TLC, DM and EA extracts showed higher tannins concentration than the HEX and M extracts. Pharmacologically, at 0.4mg/ml, DM was more effective (92 ± 6.2%) than EA (81.3 ±10%) = HEX, 77.2 ±4.7%) > M (54 ±10%) against the toxic effects of the venom. Morphologically, DM extract preserved intact 52.8% of the muscle fibers in the presence of the venom. We concluded that P. reticulata extracts are able to inhibit toxic effects of B. jararacussu venom, whose protective mechanism could be mediated by tannins.","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":" ","pages":"52-8"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/49/7e/JVR-02-052.PMC3269806.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30458087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ontogenesis, gender, and molting influence the venom yield in the spider Coremiocnemis tropix (Araneae, Theraphosidae). 个体发生、性别和蜕皮影响蜘蛛Coremiocnemis tropix(蜘蛛目，刺蛛科)的毒液产量。

Journal of Venom Research Pub Date : 2010-12-15

Volker Herzig

{"title":"Ontogenesis, gender, and molting influence the venom yield in the spider Coremiocnemis tropix (Araneae, Theraphosidae).","authors":"Volker Herzig","doi":"","DOIUrl":"","url":null,"abstract":"The demand for spider venom increases along with the growing popularity of venoms-based research. A deeper understanding of factors that influence the venom yield in spiders would therefore be of interest to both commercial venom suppliers and research facilities. The present study addresses the influence of several factors on the venom yield by systematically analyzing the data obtained from 1773 electrical milkings of the Australian theraphosid spider Coremiocnemis tropix. Gender and ontogenesis were found to cause a major effect on the venom yield, as adult female C. tropix yielded significantly more venom than adult males. During ontogenesis, the venom yield increased with increasing size of the spiders. Furthermore, a significant reduction in the venom yield during the 50-day time interval preceding a molt was found. On the other hand, extended milking intervals (up to 449 days) and different states of nutrition (as an indication of how well the spider was fed) did not significantly affect the venom yield. Overall, the present findings suggest that venom production in spiders is carefully balanced between the demand for venom and the energy costs associated with its production. It can therefore be concluded that, in line with the venom optimization hypothesis, venom is a precious resource for spiders, which have implemented control mechanisms to ensure economical venom production and usage.","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":"1 ","pages":"76-83"},"PeriodicalIF":0.0,"publicationDate":"2010-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e2/cb/JVR-01-076.PMC3086189.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29859644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deep coma and hypokalaemia of unknown aetiology following Bungarus caeruleus bites: Exploration of pathophysiological mechanisms with two case studies. 小黄鼠咬伤后的深度昏迷和原因不明的低钾血症:两个病例的病理生理机制探讨。

Journal of Venom Research Pub Date : 2010-12-14

Indika Bandara Gawarammana, Senanayake Abeysinghe Mudiyanselage Kularatne, Keerthi Kularatne, Roshita Waduge, Vajira Senaka Weerasinghe, Sunil Bowatta, Nimal Senanayake

{"title":"Deep coma and hypokalaemia of unknown aetiology following Bungarus caeruleus bites: Exploration of pathophysiological mechanisms with two case studies.","authors":"Indika Bandara Gawarammana, Senanayake Abeysinghe Mudiyanselage Kularatne, Keerthi Kularatne, Roshita Waduge, Vajira Senaka Weerasinghe, Sunil Bowatta, Nimal Senanayake","doi":"","DOIUrl":"","url":null,"abstract":"Bungarotoxin present in Bungarus caeruleus (BC) causes life threatening respiratory muscle paralysis. Deep coma and hypokalaemia have been observed in a significant proportion of patients, but the cause is unknown. We postulate the likely mechanism behind these two phenomena. We studied clinical details of two patients admitted with deep coma and performed electroencephalograms (EEG) and brain stem auditory and visual evoked potentials (BAEP and VEP). Daily serum potassium was measured along with urinary potassium excretion as a marker of total extracellular body potassium. Both patients had no brain stem reflexes on admission and the EEG revealed absent alpha and delta activity and presence of dominant theta activity. Alpha rhythm returned on the 3(rd) day in one patient, while in the other it did not, and the latter patient died on the 13(th) day due to disseminated intravascular coagulation. BAEP were delayed and VEP were absent in the deceased patient. Both had low serum potassium and low urinary potassium excretion. Replacement of potassium (up to 1.5mmol/kg/day) did not improve serum potassium and urinary potassium excretion. Absent alpha and delta activity in EEG and delayed BAEP and absent VEP are suggestive of a central action of the venom on both the cortical and brain stem neurones. Persistently low serum potassium and reduced urinary potassium excretion are suggestive of intracellular shift as the causative mechanism of hypokalaemia.","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":"1 ","pages":"71-5"},"PeriodicalIF":0.0,"publicationDate":"2010-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/87/de/JVR-01-071.PMC3086184.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29859643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in venom toxicity and antigenicity between females and males Tityus nororientalis (Buthidae) scorpions. 雌雄北方雪蝎毒液毒性和抗原性的差异。

Journal of Venom Research Pub Date : 2010-10-21

Leonardo De Sousa, Adolfo Borges, Aleikar Vásquez-Suárez, Huub Jm Op den Camp, Rosa I Chadee-Burgos, Mirna Romero-Bellorín, Jorge Espinoza, Leonardo De Sousa-Insana, Oscar Pino-García

{"title":"Differences in venom toxicity and antigenicity between females and males Tityus nororientalis (Buthidae) scorpions.","authors":"Leonardo De Sousa, Adolfo Borges, Aleikar Vásquez-Suárez, Huub Jm Op den Camp, Rosa I Chadee-Burgos, Mirna Romero-Bellorín, Jorge Espinoza, Leonardo De Sousa-Insana, Oscar Pino-García","doi":"","DOIUrl":"","url":null,"abstract":"Venom from male and female specimens of the medically important Venezuelan scorpion Tityus nororientalis have been compared. Males showed a significantly higher venom yield (2.39mg/individual) compared to female scorpions (0.98mg/individual). Female venom was significantly more toxic than that of males, with a median lethal dose (LD(50)) in C57BL/6 mice of 9.46 μg venom protein/gm body weight [95% confidence interval (8.91-9.94)] whereas LD(50) for males was 13.36(12.58-14.03) μg/gm. Mass spectral analyses by MALDI-TOF revealed differences in venom composition between males and females. From a clinical standpoint, the time course of toxicity course indicated a tendency, in the case of the female venom, to elicit the earlier occurrence of severe signs such as sialorrhea, dyspnea (bradypnea/apnea) and exophthalmus particularly in the late toxicity phase. Female venom was significantly less efficient than male venom to inhibit the binding of anti-T. discrepans antibodies to immobilized T. discrepans venom in ELISA assays, suggesting sex-related differences in the bioactive surfaces of T. nororientalis toxins. These results indicate that males and females of T. nororientalis produce venoms with different composition and activity which may have epidemiological implications.","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":"1 ","pages":"61-70"},"PeriodicalIF":0.0,"publicationDate":"2010-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/69/2f/JVR-01-061.PMC3086188.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29859642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heparin at low concentration acts as antivenom against Bothrops jararacussu venom and bothropstoxin-I neurotoxic and myotoxic actions. 低浓度的肝素可作为抗蛇毒血清，对抗鼠血蛇毒和鼠血蛇毒- 1的神经毒性和肌毒性作用。

Journal of Venom Research Pub Date : 2010-10-15

Sandro Rostelato-Ferreira, Gildo Bernardo Leite, Adélia Cristina Oliveira Cintra, Maria Alice da Cruz-Höfling, Léa Rodrigues-Simioni, Yoko Oshima-Franco

{"title":"Heparin at low concentration acts as antivenom against Bothrops jararacussu venom and bothropstoxin-I neurotoxic and myotoxic actions.","authors":"Sandro Rostelato-Ferreira, Gildo Bernardo Leite, Adélia Cristina Oliveira Cintra, Maria Alice da Cruz-Höfling, Léa Rodrigues-Simioni, Yoko Oshima-Franco","doi":"","DOIUrl":"","url":null,"abstract":"Heparin has been shown to antagonize myotoxic effects of crotaline venoms. Here a very low heparin concentration (LHC) was examined in its ability to antagonize the neurotoxic/myotoxic effects of Bothrops jararacussu venom and its phospholipase A(2) myotoxin, bothropstoxin-I (BthTX-I), in an in vitroz nerve-muscle preparation and in mice gastrocnemius. Normalization of results was done by assays with commercial antibothropic antivenom (CBA). LHC (1IU/ml) added to the incubation bath reduced by 4- and 4.5-fold (vs 2.8- and 2.5-fold by CBA) the neuromuscular paralysis, by 5.4 and 4.4-fold (vs 2.5- and 13.3-fold by CBA) the percentage of fibers damaged and by 6- and 1.7-fold (vs 30- and 1.6-fold by CBA) the CK activity induced by B. jararacussu and BthTX-I, respectively. Protamine sulphate added 15min after the incubation of the preparation with LHC+venom, avoided the LHC neutralizing effect against venom neurotoxicity. This strongly attests that given the polycationic nature of protamine, it probably complexed with the polyanionic heparin making it unattainable for binding to basic components of venom, reducing toxicity. Since heparin antagonism is generally stronger against venom effects than is myotoxin we discuss that other venom components than the BthTX-I are likely target for the antagonism promoted by the polyanionic heparin.","PeriodicalId":17653,"journal":{"name":"Journal of Venom Research","volume":"1 ","pages":"54-60"},"PeriodicalIF":0.0,"publicationDate":"2010-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c2/ae/JVR-01-054.PMC3086186.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29859641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0