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Scanning electron microscopy of normal rabbit aorta: Injury or artifact? 正常兔主动脉扫描电镜:损伤还是伪影?
Journal of ultrastructure research Pub Date : 1985-05-01 DOI: 10.1016/0889-1605(85)90067-9
Mary Richardson, Mark W.C. Hatton, Michael R. Buchanan, Sean Moore
{"title":"Scanning electron microscopy of normal rabbit aorta: Injury or artifact?","authors":"Mary Richardson,&nbsp;Mark W.C. Hatton,&nbsp;Michael R. Buchanan,&nbsp;Sean Moore","doi":"10.1016/0889-1605(85)90067-9","DOIUrl":"10.1016/0889-1605(85)90067-9","url":null,"abstract":"<div><p>The reported morphology of both normal and “injured” aortic endothelium differs significantly from one study to another. To better understand these disparities, we examined the effects of manipulating the preperfusion conditions on the subsequent morphology of normal aortic endothelium in the rabbit. Glutaraldehyde perfusion without any preperfusion with an electrolyte solution, induced vasoconstriction and the accumulation of cellular and plasma protein on the luminal surface of the aorta. Prolonged preperfusion by Ringer-Locke solution and to a lesser extent, by Krebs-Henzleit solution or modified Eagles medium, induced changes in the endothelium similar to those reported as the response to injury induced by other stimuli. Profound vessel wall alterations also occurred in animals which were shocked (through acute blood loss) or killed (by overdose of anesthetic) prior to fixation. These observations may explain, in part, the discrepancies in previous descriptions of the appearance of “normal” rabbit aortic endothelium in SEM, and suggest that the type and duration of the preperfusion must be considered to avoid morphological artifacts.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 2","pages":"Pages 159-173"},"PeriodicalIF":0.0,"publicationDate":"1985-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90067-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15199751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
Connections between the cristae and the surface of rat heart muscle mitochondria 嵴与大鼠心肌线粒体表面的联系
Journal of ultrastructure research Pub Date : 1985-04-01 DOI: 10.1016/0889-1605(85)90074-6
Fritiof S. Sjöstrand, Robert C. Candipan
{"title":"Connections between the cristae and the surface of rat heart muscle mitochondria","authors":"Fritiof S. Sjöstrand,&nbsp;Robert C. Candipan","doi":"10.1016/0889-1605(85)90074-6","DOIUrl":"10.1016/0889-1605(85)90074-6","url":null,"abstract":"<div><p>Contrary to the generally accepted rule that there are only two fracture faces associated with a membrane, the analysis of double replicas at rat heart muscle mitochondria revealed three pairs of complementary replicas with one face in each pair exposing the outer surface membrane. The replicas must then expose the surfaces of the outer surface membrane and in two of the pairs the fracture had passed between the two surface membranes in two alternative ways, either clearly between the two membranes or the fracture deviated into and through the inner surface membrane at regularly spaced intervals. This deviation reveals that at these sites the connection between the two surface membranes is particularly firm. The analysis led to the conclusion that these sites correspond to those where the stalk-like connections extending from the cristae are connected to the inner surface membrane. This way proteinaceous pathways connect the cristae to the surface of the mitochondria.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 1","pages":"Pages 38-50"},"PeriodicalIF":0.0,"publicationDate":"1985-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90074-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15169383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Morphology of the pericellular capsule in articular cartilage revealed by hyaluronidase digestion 透明质酸酶消化显示的关节软骨细胞周囊形态
Journal of ultrastructure research Pub Date : 1985-04-01 DOI: 10.1016/0889-1605(85)90071-0
C. Anthony Poole, Michael H. Flint, Brent W. Beaumont
{"title":"Morphology of the pericellular capsule in articular cartilage revealed by hyaluronidase digestion","authors":"C. Anthony Poole,&nbsp;Michael H. Flint,&nbsp;Brent W. Beaumont","doi":"10.1016/0889-1605(85)90071-0","DOIUrl":"10.1016/0889-1605(85)90071-0","url":null,"abstract":"<div><p>To allow a more valid comparison between our previous ultrastructural data and the immunolocalization of type IX and other minor collagen species in cryosectioned cartilage, we examined both normal and testicular hyaluronidase-digested canine tibial cartilage by electron microscopy. Removal of matrix proteoglycans caused the pericellular capsule to collapse against the cell surface, suggesting that its normal anatomical position is mediated by pericellular matrix hydration. Detailed examination of the pericellular capsule and pericellular channel revealed fine, faintly banded fibrils and an amorphous component somewhat similar in structure to basement membrane collagens. Matrix vesicle and the electron-dense material of the interterritorial matrix were only partially digested by hyaluronidase. We propose that the pericellular capsule is composed of a “felt-like” network of minor collagen species which act synergistically to maintain both the composition of the pericellular matrix and the integrity of the chondrocyte/pericellular matrix complex during compressive loading.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 1","pages":"Pages 13-23"},"PeriodicalIF":0.0,"publicationDate":"1985-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90071-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15170482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 33
Morphometry of the amount of smooth muscle cells in the media of various rabbit arteries 兔各种动脉介质中平滑肌细胞数量的形态测定
Journal of ultrastructure research Pub Date : 1985-04-01 DOI: 10.1016/0889-1605(85)90070-9
Odile Mathieu-Costello , Kitty Fronek
{"title":"Morphometry of the amount of smooth muscle cells in the media of various rabbit arteries","authors":"Odile Mathieu-Costello ,&nbsp;Kitty Fronek","doi":"10.1016/0889-1605(85)90070-9","DOIUrl":"10.1016/0889-1605(85)90070-9","url":null,"abstract":"<div><p>Our objective in this study was to evaluate the relative amount of smooth muscle cells in the medial layer of various rabbit arteries. The fixation of smooth muscle cells in the arterial wall is difficult and the differential effect of glutaraldehyde (GA) and fixative vehicle on cell ultrastructure in different tissues is controversial. We compared the effect of various concentrations of the vehicle and glutaraldehyde (osmolarity ranges for total fixative, 350–1030 mOsm) on the arterial wall ultrastructure. We found that a 600 mOsm GA solution (isotonic vehicle; 2.5% GA) adequately preserves arterial wall structures. The relative amount of smooth muscle cells in the media differed in various segments along the arterial tree. It ranged from 35% (thoracic aorta) to 74% (tibial artery). The importance of weighting the contractile response of different arteries<em>in vitro</em> to their relative smooth muscle cell content is discussed.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 1","pages":"Pages 1-12"},"PeriodicalIF":0.0,"publicationDate":"1985-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90070-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15047559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
An analysis of the structure of the bilaminar lamellae of the hamster egg 仓鼠卵双层片状结构的分析
Journal of ultrastructure research Pub Date : 1985-04-01 DOI: 10.1016/0889-1605(85)90073-4
D.P.L. Green
{"title":"An analysis of the structure of the bilaminar lamellae of the hamster egg","authors":"D.P.L. Green","doi":"10.1016/0889-1605(85)90073-4","DOIUrl":"10.1016/0889-1605(85)90073-4","url":null,"abstract":"<div><p>The unfertilized hamster egg contains a ubiquitous distribution of lamellate structures. Normally the lamellae are bilaminar, although occasionally both single sheets and multiple sheets are seen. Detailed analysis of the structure of a single bilaminar lamella shows that each sheet of the two-sheet structure is identical and made up of repeating rhombohedral units whose principal axes are in the ratio of 2:1. Each sheet lies at 90° to the other. Using this information, a single bilaminar lamella has been reconstructed. This reconstruction accounts for its major ultrastructural features.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 1","pages":"Pages 30-37"},"PeriodicalIF":0.0,"publicationDate":"1985-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90073-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15169382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
The effect of preparative protocol on the cytoskeleton ultrastructure observed in extracted whole-mount BHK cells 制备方案对提取的BHK细胞骨架超微结构的影响
Journal of ultrastructure research Pub Date : 1985-04-01 DOI: 10.1016/0889-1605(85)90076-X
M.J. Borrelli , S. Koehm , C.A. Cain , W.A.F. Tompkins
{"title":"The effect of preparative protocol on the cytoskeleton ultrastructure observed in extracted whole-mount BHK cells","authors":"M.J. Borrelli ,&nbsp;S. Koehm ,&nbsp;C.A. Cain ,&nbsp;W.A.F. Tompkins","doi":"10.1016/0889-1605(85)90076-X","DOIUrl":"10.1016/0889-1605(85)90076-X","url":null,"abstract":"<div><p>Extracted BHK cells (baby hamster kidney) were prepared for electron microscopy by air-drying (with Freon 113), critical-point-drying, and freeze-drying. Variations in the drying procedures had a marked effect on the resultant cytoskeleton ultrastructure. Air drying had to be done in a Freonsaturated atmosphere, residual water had to be removed from the dehydrating solutions and carbon dioxide for critical-point-dried specimens,and freeze-drying had to be done at temperatures lower than −90°C. Failure to exercise these precautions resulted in a cytoskeleton ultrastructure artifact, possibly caused by of the cytoskeleton filaments.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 1","pages":"Pages 57-65"},"PeriodicalIF":0.0,"publicationDate":"1985-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90076-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15019065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Mica sandwich technique for preparing macromolecules for rotary shadowing 制备旋转遮蔽大分子的云母夹层技术
Journal of ultrastructure research Pub Date : 1985-04-01 DOI: 10.1016/0889-1605(85)90077-1
A. Paul Mould, David F. Holmes, Karl E. Kadler , John A. Chapman
{"title":"Mica sandwich technique for preparing macromolecules for rotary shadowing","authors":"A. Paul Mould,&nbsp;David F. Holmes,&nbsp;Karl E. Kadler ,&nbsp;John A. Chapman","doi":"10.1016/0889-1605(85)90077-1","DOIUrl":"10.1016/0889-1605(85)90077-1","url":null,"abstract":"<div><p>The sandwich technique, in which a drop of sample solution is spread into a thin layer between two pieces of freshly cleaved mica, is a simple-to-use alternative to sprayng for depositing macromolecules onto mica. Test specimens of collagen molecules and actin filaments were found to suffer less shear-induced damage, they were more uniformly distributed, and only very small sample volumes were needed. Either drying from a glycerol solution (40–70% v/v) or freeze-drying can be employed. Glycerol-drying is simpler, but freeze-drying may offer better preservation of supramolecular assemblies.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 1","pages":"Pages 66-76"},"PeriodicalIF":0.0,"publicationDate":"1985-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90077-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15019066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 62
Crystallographical analysis of tooth enamel using milligram samples 用毫克样品对牙釉质进行晶体学分析
Journal of ultrastructure research Pub Date : 1985-04-01 DOI: 10.1016/0889-1605(85)90078-3
Toshiro Sakae, Ayako Okuda
{"title":"Crystallographical analysis of tooth enamel using milligram samples","authors":"Toshiro Sakae,&nbsp;Ayako Okuda","doi":"10.1016/0889-1605(85)90078-3","DOIUrl":"10.1016/0889-1605(85)90078-3","url":null,"abstract":"<div><p>An X-ray diffraction microanalytical method, in which sample is loaded onto a silver membrane filter, was applied to assess the crystal content in tooth enamel. Each enamel powder was first examined at room temperature, and then examined again at intervals after heating to 200, 400, 600, 800, and 1000°C. The hydroxyapatite composition weight and crystal weight of the samples were derived from the standard calibration curves. The “crystal content ratio” was defiend as the ratio of crystal weight to sample weight. The following results were obtained: (1) β-tricalcium phosphate(β-TCP) replaced the hydroxyapatite after heating at the high temperatures: (2) the “crystal content ratio” in the tooth enamel increased with the rise in temperature; and (3) the lattice parameters of the enamel apatite and the β-TCP were changed by the heating. The X-ray diffraction technique has the potential to analyze the crystal content using milligram samples.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 1","pages":"Pages 77-81"},"PeriodicalIF":0.0,"publicationDate":"1985-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90078-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14131075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Ultrastructure of the membranous layers enveloping the cell of the coccolithophoridEmiliania huxleyi 胡氏艾美耳球虫细胞膜层的超微结构
Journal of ultrastructure research Pub Date : 1985-04-01 DOI: 10.1016/0889-1605(85)90072-2
P. van der Wal , J.J.M. Leunissen-Bijvelt , A.J. Verkleij
{"title":"Ultrastructure of the membranous layers enveloping the cell of the coccolithophoridEmiliania huxleyi","authors":"P. van der Wal ,&nbsp;J.J.M. Leunissen-Bijvelt ,&nbsp;A.J. Verkleij","doi":"10.1016/0889-1605(85)90072-2","DOIUrl":"https://doi.org/10.1016/0889-1605(85)90072-2","url":null,"abstract":"<div><p><em>Emiliania huxleyi</em> is a marine unicellular alga that is covered by one or several layers of coccoliths (discs of crystalline calcium carbonate) or of organic scales. Immediately proximal to these layers are several membranous layers that surround the protoplast. In this study an ultrastructural analysis of this membranous envelope is presented using ultrathin-section and freeze-fracture electron microscopy. We found that the protoplast of<em>E. huxleyi</em> is surrounded in a proximal-to-distal direction by a membrane studded with intramembranous particles (IMPs), an intermediate structure consisting of several smooth layers, and an outermost membrane studded with IMPs. On freeze-fracturing the intermediate structure reveals two or three closely apposed smooth fracture faces. It is possible that this structure consists of two or three membranes that are devoid of IMPs. However, the possibility is also discussed that this structure is composed of three or four lipid monolayers so closely stacked that water is hardly or not present between them.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 1","pages":"Pages 24-29"},"PeriodicalIF":0.0,"publicationDate":"1985-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90072-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72251847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Ultrastructure of the membranous layers enveloping the cell of the coccolithophoridEmiliania huxleyi huxleyi球石藻细胞膜层的超微结构
Journal of ultrastructure research Pub Date : 1985-04-01 DOI: 10.1016/0889-1605(85)90072-2
P. Wal, J. Leunissen-Bijvelt, A. Verkleij
{"title":"Ultrastructure of the membranous layers enveloping the cell of the coccolithophoridEmiliania huxleyi","authors":"P. Wal, J. Leunissen-Bijvelt, A. Verkleij","doi":"10.1016/0889-1605(85)90072-2","DOIUrl":"https://doi.org/10.1016/0889-1605(85)90072-2","url":null,"abstract":"","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"13 1","pages":"24-29"},"PeriodicalIF":0.0,"publicationDate":"1985-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86357167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
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