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Structural modifications in the extracellular investment ofNostoc commune Vauch. during the life cycle I. Motile and nonmotile normogonium, biseriate stage 植物胞外投资的结构改变。活动和非活动正原体，双生期
Journal of ultrastructure research Pub Date : 1985-05-01 DOI: 10.1016/0889-1605(85)90068-0
G. Bazzichelli, N. Abdelahad, Franco Ventola
{"title":"Structural modifications in the extracellular investment ofNostoc commune Vauch. during the life cycle I. Motile and nonmotile normogonium, biseriate stage","authors":"G. Bazzichelli, N. Abdelahad, Franco Ventola","doi":"10.1016/0889-1605(85)90068-0","DOIUrl":"https://doi.org/10.1016/0889-1605(85)90068-0","url":null,"abstract":"","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"111 1","pages":"174-181"},"PeriodicalIF":0.0,"publicationDate":"1985-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84097429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Effects of vinblastine and colchicine on the rat adrenal cortex: Morphometric and cytochemical studies 长春碱和秋水仙碱对大鼠肾上腺皮质的影响:形态计量学和细胞化学研究
Journal of ultrastructure research Pub Date : 1985-05-01 Epub Date: 2004-11-30 DOI: 10.1016/0889-1605(85)90066-7
Maria C. Magalha˜es, A. Bonito Vitor, M.M. Magalha˜es
{"title":"Effects of vinblastine and colchicine on the rat adrenal cortex: Morphometric and cytochemical studies","authors":"Maria C. Magalha˜es,&nbsp;A. Bonito Vitor,&nbsp;M.M. Magalha˜es","doi":"10.1016/0889-1605(85)90066-7","DOIUrl":"10.1016/0889-1605(85)90066-7","url":null,"abstract":"<div><p>The effects of administration of anti-microtubular drugs—vinblastine and colchicine—on the ultrastructure of the zona fasciculata cells of young rat adrenal were studied. Young male rats were injected with vinblastine and sacrificed 2 hr later or with colchicine and sacrificed 3 hr after drug administration. Animals injected with isotonic saline in same experimental conditions served as controls. Ultrastructural alterations provoked by both drugs, vinblastine or colchicine, were identical and were most prominent in the Golgi areas. They appeared enlarged and crowded with round, or slightly elongated light vesicles, acid phosphatase, and osmium negatives. The Golgi dictyosomes, although keeping their normal morphology, were less numerous and presented cisternae which were narrower and shorter than controls. Electron-dense vesicles, round or elongated, and acid phosphatase positive—lysosomes—were observed in great number in the Golgi areas, intermingled with light vesicles. The relative volume of light vesicles and lysosomes of the treated animals was significantly increased when compared with controls, but the relative volume of dictyosomes was significantly decreased. Also the numerical density of light vesicles and lysosomes of the injected rats was significantly increased when compared with controls. These alterations are highly suggestive of the Golgi involvement in the adrenal secretory process.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 2","pages":"Pages 149-158"},"PeriodicalIF":0.0,"publicationDate":"1985-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90066-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13566009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Structural modifications in the extracellular investment ofNostoc commune Vauch. during the life cycle I. Motile and nonmotile normogonium, biseriate stage 诺托克公社Vauch细胞外投资的结构修饰。在生命周期中I.运动和非运动的正常性腺，双列期
Journal of ultrastructure research Pub Date : 1985-05-01 Epub Date: 2004-11-30 DOI: 10.1016/0889-1605(85)90068-0
Giorgio Bazzichelli , Nadia Abdelahad , Franco Ventola
{"title":"Structural modifications in the extracellular investment ofNostoc commune Vauch. during the life cycle I. Motile and nonmotile normogonium, biseriate stage","authors":"Giorgio Bazzichelli ,&nbsp;Nadia Abdelahad ,&nbsp;Franco Ventola","doi":"10.1016/0889-1605(85)90068-0","DOIUrl":"https://doi.org/10.1016/0889-1605(85)90068-0","url":null,"abstract":"<div><p>Electron microscopic observations carried out on<em>Nostoc commune</em> Vauch. in culture show that during development the extracellular investment undergoes evident modifications in size and structure. Since the investment of the motile hormogonium shows the characteristics of a “slime” and those of the nonmotile hormogonium and the biseriate stage show the characteristics of a “sheath,” the meaning of these two terms is discussed. In the slime, the existence of a reticular structure with polygonal meshes is recognized and is to be considered the structural basis of the organization of the investment. The transformation from slime to sheath takes place through a gradual diminution in the size of the meshes with a consequent increase of the fibril aggregation. The possibility is considered that slime-sheath reversibility as well as cessation of motility are related with modifications in the cellular secretion.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 2","pages":"Pages 174-181"},"PeriodicalIF":0.0,"publicationDate":"1985-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90068-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72215328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Sputter shadowing improved by using a tungsten target 使用钨靶改善了溅射阴影
Journal of ultrastructure research Pub Date : 1985-05-01 Epub Date: 2004-11-30 DOI: 10.1016/0889-1605(85)90065-5
William R. Colquhoun, Lynne U. Cassimeris
{"title":"Sputter shadowing improved by using a tungsten target","authors":"William R. Colquhoun,&nbsp;Lynne U. Cassimeris","doi":"10.1016/0889-1605(85)90065-5","DOIUrl":"10.1016/0889-1605(85)90065-5","url":null,"abstract":"<div><p>This work builds upon a previous paper (<span>W. Colquhoun, 1984</span>,<em>J. Ultrastruct. Res.</em><strong>87</strong>, 97) in which a sputter shadowing device was briefly described. The device allowed TEM specimens to be shadowed in a conventional sputter coater. Images obtained by sputter shadowing with a standard Au/Pd target were of good quality but were slightly inferior to the best that could be obtained by e<sup>−</sup>-beam evaporation of tungsten. Here we show that construction and use of a tungsten target greatly improves the quality of the sputter shadowed deposit. Images of DNA and ribosomal subunits contrasted by sputter shadowing with tungsten are shown. The DNA images indicate that sputter shadowing with tungsten is a gentle contrasting technique. The sputter shadowed images of the 30 S ribosomal subunits show the major features of the particle revealed by evaporation shadowing using the most sophisticated of methods in that technology. Advantages of sputter shadowing are discussed and a rationale for the improved grain obtained by sputtering tungsten is suggested.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 2","pages":"Pages 138-148"},"PeriodicalIF":0.0,"publicationDate":"1985-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90065-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14072194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Antikeratin antibody staining on ultrathin sections of epidermal cells prepared by low-denaturation embedding 低变性包埋法制备的表皮细胞超薄切片的抗角蛋白抗体染色
Journal of ultrastructure research Pub Date : 1985-05-01 Epub Date: 2004-11-30 DOI: 10.1016/0889-1605(85)90061-8
Kenneth D. Marenus
{"title":"Antikeratin antibody staining on ultrathin sections of epidermal cells prepared by low-denaturation embedding","authors":"Kenneth D. Marenus","doi":"10.1016/0889-1605(85)90061-8","DOIUrl":"10.1016/0889-1605(85)90061-8","url":null,"abstract":"<div><p>Direct immunological identification of cellular components has not been possible in tissues prepared for electron microscopy by conventional methods. This may be attributed, in part, to the relatively harsh reagents employed. Using an approach to preparation of biological specimens for transmission electron microscopy that aims for minimal perturbation of native protein conformation, we have obtained specimens that may be stained with antibodies. Recent investigations using these methods have revealed new information regarding the organization of epidermal cells.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 2","pages":"Pages 92-103"},"PeriodicalIF":0.0,"publicationDate":"1985-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90061-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13566010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Centriole size modifications during the cell cycle of the amoebae of the myxomycetePhysarum polycephalum 黏菌多头绒泡菌变形虫细胞周期中中心粒大小的改变
Journal of ultrastructure research Pub Date : 1985-05-01 Epub Date: 2004-11-30 DOI: 10.1016/0889-1605(85)90064-3
C. Gely, M. Wright
{"title":"Centriole size modifications during the cell cycle of the amoebae of the myxomycetePhysarum polycephalum","authors":"C. Gely,&nbsp;M. Wright","doi":"10.1016/0889-1605(85)90064-3","DOIUrl":"https://doi.org/10.1016/0889-1605(85)90064-3","url":null,"abstract":"<div><p>Anterior and posterior centrioles of<em>Physarum</em> amoebae are indistinguishable by their size during interphase but there is a correlation between the size of the two centrioles in the same amoeba. The interphase length of centrioles in diploid amoebae possessing only one pair of centrioles was 11% longer than in the case of the haploid strain. Treatment with taxol led to a 23 and 32% increase of the mean length in interphase and blocked mitosis, respectively. Conversely, during control mitosis the parental centrioles showed a 12% decrease of their mean length while the size of the daughter centrioles increased progressively. Neither nocodazole nor cold treatment induce a decrease of centriole length. The mean length of the cartwheel structure (internal proximal part) although constant during mitosis could be increased 24% in the presence of taxol. Similarly there was a correlation between the number of anterior satellites and the centriole length.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 2","pages":"Pages 127-137"},"PeriodicalIF":0.0,"publicationDate":"1985-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90064-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72215327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Distribution of filipin-sterol complexes in the myelinated nerve fiber 菲律宾甾醇复合物在有髓神经纤维中的分布
Journal of ultrastructure research Pub Date : 1985-05-01 Epub Date: 2004-11-30 DOI: 10.1016/0889-1605(85)90062-X
G. Allt , C.E. Blanchard , M.L. MacKenzie , K. Sikri
{"title":"Distribution of filipin-sterol complexes in the myelinated nerve fiber","authors":"G. Allt ,&nbsp;C.E. Blanchard ,&nbsp;M.L. MacKenzie ,&nbsp;K. Sikri","doi":"10.1016/0889-1605(85)90062-X","DOIUrl":"10.1016/0889-1605(85)90062-X","url":null,"abstract":"<div><p>Using filipin as a cytochemical probe to reveal the distribution of cholesterol, myelinated peripheral nerve fibers were examined in freeze-fracture replicas. Filipin-sterol complexes were most abundant in the Schwann cell and axonal plasma membranes. In the Schwann cell plasma membrane there was no heterogeneity in complex distribution in relation to the subjacent cytoplasmic network. In myelin lamellae there was a decrease in complexes from outer to inner lamellae and some aggregation of complexes in individual lamellae. The density of complexes in cytoplasmic organelles varied from absent in mitochondria to high in lysosome-like bodies. The results are interpreted in terms of the related biochemical composition and biophysical properties of cell membranes, with particular reference to the myelinated nerve fiber. The influence of diffusion barriers and gradients on the formation of complexes by filipin is considered.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 2","pages":"Pages 104-111"},"PeriodicalIF":0.0,"publicationDate":"1985-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90062-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15198042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Microtubules and macrotubules in fish meiosis 鱼类减数分裂中的微管和大管
Journal of ultrastructure research Pub Date : 1985-05-01 Epub Date: 2004-11-30 DOI: 10.1016/0889-1605(85)90060-6
Xavier Mattei, Raymond Romand, Omar Thiom Thiaw
{"title":"Microtubules and macrotubules in fish meiosis","authors":"Xavier Mattei,&nbsp;Raymond Romand,&nbsp;Omar Thiom Thiaw","doi":"10.1016/0889-1605(85)90060-6","DOIUrl":"10.1016/0889-1605(85)90060-6","url":null,"abstract":"<div><p>During meiosis in the male of a cyprinodontid fish,<em>Aphyosemion splendopleure</em>, and during the organization of the spindle of division, the spindle is made of two types of tubules: microtubules (20–25 nm) and macrotubules (30–50 nm). The macrotubules are associated only with the polar region of the meiotic apparatus and are located outside the spindle of microtubules. At the end of meiosis, the spindle microtubules depolymerize whereas the macrotubules remain. One can find them throughout the entire process of spermiogenesis; later, they disappear only at the end of spermatid maturation. We have studied four populations from Cameroon, three of them with macrotubules.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 2","pages":"Pages 83-91"},"PeriodicalIF":0.0,"publicationDate":"1985-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90060-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15199752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Ultrastructural visualization of unfixed and unstained whole mounts by high-voltage electron microscopy at low temperatures 低温高压电镜观察未固定和未染色整片的超微结构
Journal of ultrastructure research Pub Date : 1985-05-01 Epub Date: 2004-11-30 DOI: 10.1016/0889-1605(85)90063-1
Mircea Fotino, Thomas H. Giddings
{"title":"Ultrastructural visualization of unfixed and unstained whole mounts by high-voltage electron microscopy at low temperatures","authors":"Mircea Fotino,&nbsp;Thomas H. Giddings","doi":"10.1016/0889-1605(85)90063-1","DOIUrl":"10.1016/0889-1605(85)90063-1","url":null,"abstract":"<div><p>A physical procedure for the visualization of cellular fine structures is described as an alternative to chemical preparative techniques. It consists of fixation by fast freezing followed by controlled etching in the cryostage of a million-volt transmission electron microscope. Whole mounts were thus observed under stable conditions with no use of chemical fixatives, solvents, or stains, with no exposure to the atmosphere, and with the improved penetration and resolution in thick specimens that characterize high-voltage electron microscopy. The preservation, contrast, and resolution exhibited by images of preparations obtained by this procedure are discussed.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 2","pages":"Pages 112-126"},"PeriodicalIF":0.0,"publicationDate":"1985-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90063-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15199750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Scanning electron microscopy of normal rabbit aorta: Injury or artifact? 正常兔主动脉扫描电镜:损伤还是伪影?
Journal of ultrastructure research Pub Date : 1985-05-01 Epub Date: 2004-11-30 DOI: 10.1016/0889-1605(85)90067-9
Mary Richardson, Mark W.C. Hatton, Michael R. Buchanan, Sean Moore
{"title":"Scanning electron microscopy of normal rabbit aorta: Injury or artifact?","authors":"Mary Richardson,&nbsp;Mark W.C. Hatton,&nbsp;Michael R. Buchanan,&nbsp;Sean Moore","doi":"10.1016/0889-1605(85)90067-9","DOIUrl":"10.1016/0889-1605(85)90067-9","url":null,"abstract":"<div><p>The reported morphology of both normal and “injured” aortic endothelium differs significantly from one study to another. To better understand these disparities, we examined the effects of manipulating the preperfusion conditions on the subsequent morphology of normal aortic endothelium in the rabbit. Glutaraldehyde perfusion without any preperfusion with an electrolyte solution, induced vasoconstriction and the accumulation of cellular and plasma protein on the luminal surface of the aorta. Prolonged preperfusion by Ringer-Locke solution and to a lesser extent, by Krebs-Henzleit solution or modified Eagles medium, induced changes in the endothelium similar to those reported as the response to injury induced by other stimuli. Profound vessel wall alterations also occurred in animals which were shocked (through acute blood loss) or killed (by overdose of anesthetic) prior to fixation. These observations may explain, in part, the discrepancies in previous descriptions of the appearance of “normal” rabbit aortic endothelium in SEM, and suggest that the type and duration of the preperfusion must be considered to avoid morphological artifacts.</p></div>","PeriodicalId":17593,"journal":{"name":"Journal of ultrastructure research","volume":"91 2","pages":"Pages 159-173"},"PeriodicalIF":0.0,"publicationDate":"1985-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0889-1605(85)90067-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15199751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
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