Journal of the Reticuloendothelial Society最新文献_第2页

Bursal and thymic reticular epithelial cells in the chicken: induction of B- and T-lymphocyte differentiation by in vitro monolayer cultures. 鸡法氏囊和胸腺网状上皮细胞:通过体外单层培养诱导B淋巴细胞和t淋巴细胞分化。

Journal of the Reticuloendothelial Society Pub Date : 1983-11-01

R L Boyd, H A Ward, H K Muller

{"title":"Bursal and thymic reticular epithelial cells in the chicken: induction of B- and T-lymphocyte differentiation by in vitro monolayer cultures.","authors":"R L Boyd, H A Ward, H K Muller","doi":"","DOIUrl":"","url":null,"abstract":"The role of chicken bursal and thymic reticular epithelial cells (REp cells) in the induction of B- and T-lymphocyte differentiation has been investigated using in vitro culture of these cells. Splenic adherent cells were used as controls. Precursor populations were obtained predominantly from embryonic bone marrow or spleen and incubated with REp cell monolayers for 72 hr. Differentiation was assessed by the expression of specific membrane antigens: chicken B lymphocyte-specific antigen (CBLA) and chicken T lymphocyte-specific antigen (CTLA). Bursal REp cells specifically induced approximately 20-30% of the embryonic cells to express CBLA but had no influence on CTLA expression. Conversely, CTLA, but not CBLA, was induced in approximately 20% of the embryonic cells by the thymic REp cell cultures. No effect on the expression of either antigen was observed with the splenic adherent cells. The induced cells correspond to the first stage of B- and T-lymphocyte differentiation found in vivo. The assay system also facilitated the study of shifts in precursor content of various organs with development. Whereas embryonic bone marrow of all ages studied contained both B- and T-lymphocyte precursors, these were not demonstrable in donors after hatching. Use of 12-day embryonic bursa and thymus suspensions as sources of stem cells indicated that precommitment to the B- or T-lymphocyte lineage is irreversible.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"34 5","pages":"383-93"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17476046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bursal and thymic reticular epithelial cells in the chicken: preparation of in vitro monolayer cultures. 鸡法氏囊和胸腺网状上皮细胞:体外单层培养的制备。

Journal of the Reticuloendothelial Society Pub Date : 1983-11-01

R L Boyd, H A Ward, H K Muller

{"title":"Bursal and thymic reticular epithelial cells in the chicken: preparation of in vitro monolayer cultures.","authors":"R L Boyd, H A Ward, H K Muller","doi":"","DOIUrl":"","url":null,"abstract":"To study the nature of reticular epithelial (REp) cells and their role in the specific microenvironments of the chicken bursa and thymus, a method was developed for the in vitro culture of purified preparations of these cells. For comparison, similar cultures of splenic adherent cells were also performed. REp cell-rich bursa medullary follicles and mildly trypsinized thymic fragments were X-irradiated (850 rad) to eliminate remaining lymphocytes and transferred to culture flasks. In bursal cultures, after 2-4 days incubation the basement membrane (BM) encapsulating the follicles disrupted and the immediately underlying epithelial cells grew out as a monolayer. By 10 days, REp cells at the periphery developed cytoplasmic processes; occasionally these cells appeared to \"bud-off\" and grow as isolated dendritic cells. Thymic REp cells were generally slower to proliferate but formed a monolayer by 10-14 days. Splenic adherent cells developed extensive growth within 4 days. REp cells were distinguished from fibroblasts, when present, morphologically and by their limited phagocytic ability. The former were also periodic acid-Sciffs reagent (PAS)-positive and produced reticulin granules. Bursal REp cells were also positive for a gut-associated mucin, but this may have been bound in vivo prior to culture. Neither T nor B lymphocyte-specific antigens were detectable on the cultured REp cells or splenic adherent cells, but they were all rich in cytoplasmic actin. A major feature of REp cells to emerge in this study was the obvious presence of subpopulations of these cells, which raises important questions as to their exact nature and lineage. The accompanying paper details the ability of the bursal and thymic REp cell cultures to induce B-or T-lymphocyte differentiation, respectively, in vitro.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"34 5","pages":"371-82"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17662389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of cellular and humoral mechanisms of carbaryl-induced reticuloendothelial phagocytic depression. 西巴威诱导网状内皮细胞吞噬抑制的细胞和体液机制的评价。

Journal of the Reticuloendothelial Society Pub Date : 1983-11-01

B Pipy, D de Maroussem, M Beraud, P Derache

{"title":"Evaluation of cellular and humoral mechanisms of carbaryl-induced reticuloendothelial phagocytic depression.","authors":"B Pipy, D de Maroussem, M Beraud, P Derache","doi":"","DOIUrl":"","url":null,"abstract":"The simultaneous injection of carbaryl and colloidal carbon phagocytized by the reticuloendothelial cells results in competition between the two substances in favor of the carbon particles. Experiments with opsonized carbaryl suggest that the decrease in carbaryl blood clearance by the colloid is mediated by a depletion of serum opsonins. Following blockade, the liver carbaryl uptake was depressed in the control group (17%), while it was increased in the opsonized group (12%). With all preparations of carbaryl, opsonized or non-opsonized, colloidal carbon produced a slight and variable increase in carbaryl uptake by the spleen and lungs. These results indicate that, besides the uptake of carbaryl by the hepatocytes, other clearance sites must also be considered such as the Kupffer cells and other liver sinusoidal cells. Moreover our results show that intravenous administration of carbaryl induces a state of phagocytic depression as indicated by impaired intravascular phagocytosis and depressed hepatic uptake of the reticuloendothelial (RE)-test colloidal suspension. The results obtained from injection of opsonized colloidal particles during carbaryl-induced RE-depression, and the fact that carbaryl and carbon are both opsonized by the same serum factor, suggest that the mechanisms of RE-blockade involve selective hepatic and splenic macrophage failure and depletion of serum opsonins. According to our enzymatic investigation, this failure of the RE system to incorporate colloids during carbaryl--RE-blockade could be due to a defect in the activity of macrophage membrane-bound serine esterase.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"34 5","pages":"395-412"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17476047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liposome-encapsulated cephalothin in the treatment of experimental murine salmonellosis. 脂质体包封头孢菌素治疗实验性小鼠沙门氏菌病。

Journal of the Reticuloendothelial Society Pub Date : 1983-10-01

J V Desiderio, S G Campbell

引用次数: 0

Resistance and susceptibility of mice to bacterial infection. IV. Functional specificity in natural resistance to facultative intracellular bacteria. 小鼠对细菌感染的抗性和易感性。对兼性胞内细菌自然抗性的功能特异性。

Journal of the Reticuloendothelial Society Pub Date : 1983-10-01

C Cheers, M Ho

{"title":"Resistance and susceptibility of mice to bacterial infection. IV. Functional specificity in natural resistance to facultative intracellular bacteria.","authors":"C Cheers, M Ho","doi":"","DOIUrl":"","url":null,"abstract":"The effect of opsonic antibody on resistance of susceptibility of three strains of mice, C57Bl/10, BALB/c, and CBA to the intracellular bacteria Listeria monocytogenes, Salmonella typhimurium, and Brucella abortus was tested. Bacteria were opsonized by serum treatment before their injection into mice, or the mice were preimmunized by injection with alcohol killed bacteria which induces antibody without macrophage activation. Antibody did not increase the rate of clearance of Listeria from the bloodstream, nor did it affect the subsequent growth of that organism in the spleen and liver. Blood clearance of S. typhimurium and of B. abortus was increased by preopsonization with specific antibody, indicating that opsonins were a limiting factor in resistance to these two bacteria. However, neither opsonization before infection nor immunization with alcohol killed vaccines had any effect on the strain distribution of resistance/susceptibility, which differs for each of the three intracellular pathogens. Thus, even in the presence of adequate opsonization the three strains of mice showed different patterns of resistance/susceptibility to Listeria, S. typhimurium, and B. abortus. This implies that each has a unique cellular mechanism of early nonspecific resistance.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"34 4","pages":"299-309"},"PeriodicalIF":0.0,"publicationDate":"1983-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17473041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human mononuclear phagocyte-associated antigens. III. Relationship of cell surface antigen phenotype between cultured monocytes and tissue macrophages. 人单核吞噬细胞相关抗原。3培养单核细胞与组织巨噬细胞细胞表面抗原表型的关系。

Journal of the Reticuloendothelial Society Pub Date : 1983-10-01

J C Waldrep, A M Kaplan, T Mohanakumar

{"title":"Human mononuclear phagocyte-associated antigens. III. Relationship of cell surface antigen phenotype between cultured monocytes and tissue macrophages.","authors":"J C Waldrep, A M Kaplan, T Mohanakumar","doi":"","DOIUrl":"","url":null,"abstract":"In order to determine the correlation between the in vitro model of monocyte differentiation and its in vivo counterparts, cell surface phenotypes of monocytes in culture and mature tissue macrophages were analyzed using monoclonal antibodies, M1/70, TA-1 anti-HLA-DR, and a heteroantisera prepared to macrophage cell line U937. Following 7 days in culture the reactivity of monocytes with M1/70 diminished from 70 +/- 9% to 31 +/- 8%. Similarly, the reactivity to TA-1 dropped from 88 +/- 8% to 23 +/- 7% and for anti-DR, from 79 +/- 5% to 41 +/- 16%. Reactivity with anti-U937 remained unchanged. This altered phenotype of cultured monocytes was found to approximate that of resident splenic macrophages (MO). In addition, freshly isolated monocytes and peritoneal exudate MO (PEMO) were found to be functionally similar in their inability to phagocytize via the C receptors. These results suggest that PEMO arriving recently into the peritoneal cavity from peripheral blood may be midway in transition between monocytes and mature MO, and that the phenotype and functional changes attributed to monocytes in culture may reflect changes that occur in vivo in the transition to tissue MO.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"34 4","pages":"323-30"},"PeriodicalIF":0.0,"publicationDate":"1983-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17675912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Some properties of the plasma membrane of macrophages from germ-free rats. 无菌大鼠巨噬细胞质膜的一些特性。

Journal of the Reticuloendothelial Society Pub Date : 1983-10-01

L Fornůsek, V Vĕtvicka, L Jarosková, R Stepánková

{"title":"Some properties of the plasma membrane of macrophages from germ-free rats.","authors":"L Fornůsek, V Vĕtvicka, L Jarosková, R Stepánková","doi":"","DOIUrl":"","url":null,"abstract":"The mobility of plasma membrane receptors for lectins concanavalin A (Con A), phytohemagglutinin (PHA), garden pea agglutinin (PSA), lentil agglutinin (LCA), peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), and perch spawn agglutinin (PFA), the presence of binding sites for sheep erythrocytes, the presence of Fc and complement receptors, as well as pinocytic and phagocytic activities were investigated in normal peritoneal macrophages from conventionally reared (CV) and germ-free (GF) rats. Differences varying according to the lectin used were found in lectin-receptor-complex lateral mobilities measured as a function of patch and cap formation. Germ-free-rat-derived macrophages showed a significant decrease in the average amount of SBA binding sites per cell as determined by 125I-SBA labeling. The percentage of complement- and Fc-receptor-bearing macrophages was lower in GF rats, in contrast to the higher percentage of macrophages forming spontaneous rosettes with sheep erythrocytes. The pinocytic activity as determined by the neutral red uptake assay exhibited a threefold increase in GF rat-derived macrophages in comparison to CV ones. On the other hand, phagocytosis was more intense in macrophages from conventional rats, as detected by the engulfing of CdCO3 microcrystals. Our results, together with other recent reports, indicate that the earlier opinion that the peritoneal macrophages of GF animals do not differ essentially from those of conventional ones needs to be revised.","PeriodicalId":17481,"journal":{"name":"Journal of the Reticuloendothelial Society","volume":"34 4","pages":"331-40"},"PeriodicalIF":0.0,"publicationDate":"1983-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17676689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppressive effects of Corynebacterium parvum on primary and secondary antibody responses in mice. 小棒状杆菌对小鼠一抗和二抗反应的抑制作用。

Journal of the Reticuloendothelial Society Pub Date : 1983-10-01

J D Ansell, K N Trail, C M McDougall, C J Inchley

引用次数: 0

Studies on the role of macrophages in Marek's disease of the chicken. 巨噬细胞在鸡马立克病中的作用研究。

Journal of the Reticuloendothelial Society Pub Date : 1983-10-01

P C Powell, K J Hartley, B M Mustill, M Rennie

引用次数: 0

Presence of the factor increasing monocytopoiesis (FIM) in rabbit peripheral blood during an acute inflammation. 兔急性炎症时外周血单核细胞增生(FIM)增加因子的存在。

Journal of the Reticuloendothelial Society Pub Date : 1983-09-01

W Sluiter, I Elzenga-Claasen, E Hulsing-Hesselink, R van Furth

引用次数: 0