Journal of the Mexican Chemical Society最新文献

{"title":"Pennyroyal Essential Oil as a Green Pesticide for Tribolium castaneum (Herbst) Management and its Effects on Substrate Quality and Acetylcholinesterase Inhibition","authors":"O. Bachrouch, Youkabed Zarroug, S. Bourgou, K. Charradi, J. Sriti, K. Msaada, Slim Jallouli, Kabas Chaibi, S. Haouel Hamdi, M. Abderraba, J. Mediouni Ben Jemâa","doi":"10.29356/jmcs.v67i2.1875","DOIUrl":"https://doi.org/10.29356/jmcs.v67i2.1875","url":null,"abstract":"Abstract. Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) is the most damaging beetle species destroying stored products. Their management are difficult because they are developing resistance to insecticide. Essential oil application as bio-pesticide is receiving renewed attention. Pennyroyal (Mentha pulegium L.) is a relevant crop in the production of EO. The insecticidal effect of Tunisian pennyroyal EO were investigated against T. castaneum adults on wheat flour substrate under different occupation space conditions after 30 and 60 days of storage. EO impacts on wheat flour quality, volatile compounds retained by the treated substrate and acetylcholinesterase (AChE) activity were examined. GC/MS analysis showed that EO major compounds was pulegone (39.15 %). Insect mortality was assessed when EO was applied at 196 µL/L air to the stored wheat flour at 50 % or 100 % container capacity. A highest mortality occurred at 50 % container capacity, with means at 32.8 % and 72.2 % after storing for 30 and 60 days, respectively. Volatile compounds retained by the treated wheat flour were characterized via Headspace analysis. Results underlined that long time of storage (mainly 60 days) allowed the retention of significant amounts of menthone. EO substrate treatment changed flour moisture and protein content. EO exhibited insecticidal activity via inhibiting acetylcholinesterase activity.\u0000 \u0000Resumen. Tribolium castaneum Herbst (Coleoptera: Tenebroideae) es la especie de escarabajo más dañina que destruye productos almacenados. Su manejo es difícil debido al desarrollo de resistencia a insecticidas. La aplicación de aceites esenciales (AE´s) como bio-pesticidas está recibiendo atención renovada. El poleo (Mentha pulegium L.) es un cultivo relevante en la producción de AE. El efecto insecticida del aceite esencial de poleo Tunecino fue investigado contra adultos de T. castaneum mantenidos en sustrato de harina de trigo bajo diferentes condiciones de espacio después de 30 y 60 días de almacenamiento. El AE impacta sobre la calidad de la harina de trigo, se evaluaron los compuestos volátiles retenidos en el sustrato tratado y la actividad de acetilcolinesterasa (ACE). El análisis por CG/EM mostró que el compuesto mayoritario en el AE fue la pulegona (39.15 %). La mortalidad de los insectos fue evaluada cuando el AE fue aplicado a 196 mL/L de aire a harina de trigo almacenada a un 50 % y 100 % de la capacidad del contenedor. La mortalidad más alta ocurrió en el contenedor a un 50 % de capacidad, con promedios de 32 % y 72.7 % después de 30 y 60 días de almacenamiento, respectivamente. Los compuestos volátiles retenidos por la harina de trigo fueron caracterizados mediante un análisis por espacio de cabeza (Headspace). Los resultados resaltaron que un tiempo largo de almacenamiento (principalmente 60 días) permite la retención de cantidades significativas de metona. El tratamiento del sustrato con AE cambió la humedad y el contenido de proteína de la hari","PeriodicalId":17377,"journal":{"name":"Journal of the Mexican Chemical Society","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83854199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial and Antioxidant activities of Algerian Juniperus phoenicea and Salvia officinalis Essential Oils","authors":"Noura Ait-Mimoune, Fatima Kada, Houda Drider","doi":"10.29356/jmcs.v67i2.1921","DOIUrl":"https://doi.org/10.29356/jmcs.v67i2.1921","url":null,"abstract":"Abstract. In this work, the chemical composition, antioxidant and antifungal activities of Juniperus phoenicea and Salvia officinalis essential oils (Eos) were evaluated. The Gas Chromatography-Mass spectrometry (GC-MS) identification of S. officinalis Eo revealed the predominance of cis-chrysanthenyl acetate (64.82 %), and α- thujone (14.7 %).  The main compounds of J. phoenicea oil were α-Pinene (64.4 %) and δ-3-Carene (7.02 %).  The antibacterial activity was evaluated using the agar well diffusion method.  The most susceptible bacteria was  Staphylococcus aureus.  The antifungal activity was tested against Aspergillus and Penicillium species by the poisoned food method. The two essential oils (Eos) exhibited an antifungal activity, with S. officinalis oil being the most potent one (8-82 % of inhibition). The antioxidant activity was characterized by the DPPH free radical scavenging method.  J. phoenicea and S.officinalis Eos had both a moderate antioxidant effect. Additionally, an antagonistic effect was observed between the Eos when used in combination.\u0000 \u0000Resumen. En este trabajo se evaluó la composición química, actividad antioxidante y antifúngica de los aceites esenciales de Juniperus phoenicea y Salvia officinalis (Eos). La identificación por cromatografía de gases-espectrometría de masas (GC-MS) de los aceites de S. officinalis reveló el predominio del cis-crisantenil acetato (64.82%) y α-tujona (14.7%). Los principales compuestos del aceite de J. phoenicea fueron α-pineno (64.4%) y el δ-3-careno (7,02 %). La actividad antibacteriana se evaluó mediante el método de difusión en pozo de agar. La bacteria más susceptible fue Staphylococcus aureus. La actividad antifúngica se probó contra especies de Aspergillus y Penicillium por el método de alimentos envenenados. Los dos aceites esenciales exhibieron actividad antifúngica, siendo el aceite de S. officinalis el más potente (8-82% de inhibición). La actividad antioxidante se caracterizó por el método de captación de radicales libres DPPH. J. phoenicea y S.officinalis tuvieron un efecto antioxidante moderado. Además, se observó un efecto antagónico entre los Eos cuando se usaban en combinación.","PeriodicalId":17377,"journal":{"name":"Journal of the Mexican Chemical Society","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75103420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Processing Stage on the Bioactives of Tomato Purees Supplemented Preserved by Microwaves","authors":"Gabriel Ruiz Flores, Maricela Quiroz-Bravo, Mario Márquz Lemus, D. M. Hernández-Martínez, M. López-Cortez","doi":"10.29356/jmcs.v67i2.1877","DOIUrl":"https://doi.org/10.29356/jmcs.v67i2.1877","url":null,"abstract":"Abstract. Tomatoes are a natural source of bioactive compounds. Most of these substances accumulate in the tomato skin and seeds and are scarce in the pulp, which is the main ingredient of tomato products. The application of technological processes as well as plant variety, cultivation, and harvesting conditions, can significantly affect the content and composition of phenolics in tomato fruit and tomato-based products. To increase the content of bioactive, in the present investigation a tomato puree supplemented with the seeds and skin of the fruit was prepared using tomatoes of Mexican origin. The bioactive compounds at each stage of the process were evaluated. The variation in bioactive compounds occurred during the tomato juice concentration stage. The retention of bioactive compounds was similar when conventional pasteurization and microwave treatment were applied independently, however, the microwave used a lower time. Tomato puree supplemented with seeds and skin presented a higher content of bioactive. There were no significant differences in the microbiological activity between pasteurization and microwave treatment since both heat treatments achieved similar microbial inactivation. Chlorogenic acid, rutin, resveratrol, quercetin, and naringenin were identified by HPLC. The microwave-treated tomato puree presented a less acidic taste, a more intense red color, and a more pleasant aroma compared to the pasteurized tomato puree. The PCA analysis showed that the processing stages that affect the polyphenolic content in the elaboration of tomato purees corresponded to the heat treatments appliednd and the concentration stage.\u0000 \u0000Resumen. Los tomates son una fuente natural de bioactivos. La mayoría de estas sustancias se acumulan en la piel y las semillas del tomate y son escasas en la pulpa, que es el ingrediente principal de los productos derivados del tomate. La aplicación de procesos tecnológicos, así como la variedad de plantas, el cultivo y las condiciones de cosecha, pueden afectar significativamente el contenido y la composición de compuestos fenólicos en el fruto del tomate y los productos a base de tomate. Para aumentar el contenido de bioactivos, en la presente investigación se preparó un puré de tomate suplementado con las semillas y la piel del fruto utilizando tomates de origen mexicano. Se evaluaron los compuestos bioactivos en cada etapa del proceso. La variación de los compuestos bioactivos ocurrió durante la etapa de concentración del jugo de tomate. La retención de compuestos bioactivos fue similar cuando la pasteurización convencional y el tratamiento con microondas se aplicaron de forma independiente, sin embargo, el microondas usó un tiempo menor. El puré de tomate suplementado con semillas y piel presentó mayor contenido de bioactivos. No hubo diferencias significativas en la actividad microbiológica entre la pasteurización y el tratamiento con microondas ya que ambos tratamientos térmicos lograron una inactivación micro","PeriodicalId":17377,"journal":{"name":"Journal of the Mexican Chemical Society","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85650208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Conditions for Ultrasound-Assisted Extraction of the Betulinic Acid, Oleanolic Acid, and Ursolic Acid from the Jujube using Response Surface Methodology (RSM)","authors":"Zh. Khoshsima, A. A. Sharif, A. Akrami","doi":"10.29356/jmcs.v67i1.1862","DOIUrl":"https://doi.org/10.29356/jmcs.v67i1.1862","url":null,"abstract":"Pentacyclic triterpenic acids have potential effects in treating human diseases. Thus, it seems necessary to have an effective method to extract and separate triterpenic acids from plants and fruits such as jujube. To this end, this study optimized ultrasound-assisted extraction of Betulinic acid (BA), Oleanolic acid (OA), and Ursolic acid (UA) from Iranian jujube using response surface methodology (RSM) and quantified by high-performance liquid chromatography (HPLC). Box-Behnken Design (BBD) was used to model the response surface. The selected independent variables were ultrasonic bath temperature (T), sonication time (θ), and liquid to solid ratio (α). The P-value and R-squared (R2) for all extraction efficiencies indicated a good correlation between the experimental results and those predicted by the quadratic model. The analysis of variance (ANOVA) results showed the significant impact of linear coefficients (T, θ, α), quadratic coefficients (T2, θ2, α2), and interaction coefficients (Tθ, Tα, θα) of the model on the extraction of three triterpenic acids. The predicted optimal temperature, sonication time, and liquid to solid ratio were 40.34 °C, 34.63 min, and 14.85 mL/g. The maximum yields for BA, OA, and UA were 304.14, 170.61, and 195.23 µg/g, respectively. Extraction was carried out by the calculated rounded up optimal values of T=40 °C, θ=35 min, and α=15 mL/g. The extraction efficiencies for BA, OA, and UA were 303.83±0.85, 169.52±0.86, and 195.84±0.75 µg/g, respectively. These results were comparable to those calculated under model-optimized conditions, indicating the accuracy of our model.\u0000 \u0000Resumen. Los ácidos triterpénicos pentacíclicos tienen potencialmente efectos en el tratamiento de enfermedades humanas. Por ello es necesario disponer de un método eficaz para extraer y separar los ácidos triterpénicos de plantas y frutos como el jujube (Ziziphus jujuba). Con este fin, en este estudio se optimizó la extracción asistida por ultrasonido de los ácidos betulínico (BA), oleanólico (OA) y ursólico (UA) del jujube iraní utilizando la metodología de superficie de respuesta (RSM) y cuantificada por cromatografía líquida de alta resolución (HPLC). Se utilizó el diseño Box-Behnken Design (BBD) para modelar la superficie de respuesta. Las variables independientes seleccionadas fueron la temperatura del baño ultrasónico (T), el tiempo de sonicación (θ) y la proporción de líquido a sólido (α). El valor P y R-cuadrática (R2) para todas las eficiencias de extracción indicaron una buena correlación entre los resultados experimentales y los predichos por el modelo cuadrático. Los resultados del análisis de varianza (ANOVA) mostraron el impacto significativo de los coeficientes lineales (T, θ, α), los coeficientes cuadráticos (T2, θ2, α2) y los coeficientes de interacción (Tθ, Tα, θα) del modelo en la extracción de los tres ácidos triterpénicos. La temperatura óptima predicha, el tiempo de sonicación y la proporción de líquido a sólido fueron 40.34","PeriodicalId":17377,"journal":{"name":"Journal of the Mexican Chemical Society","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76114263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved Knoevenagel Condensation Protocol for the Synthesis of Cyanoacrylates and their Anticancer Activity","authors":"Hari Babu Bolikolla, Santha Kumari Merugu","doi":"10.29356/jmcs.v67i1.1835","DOIUrl":"https://doi.org/10.29356/jmcs.v67i1.1835","url":null,"abstract":"DIPEAc (diisopropylethylammonium acetate) has been used as a catalyst in the Knoevenagel condensation of aldehydes and ethyl cyanoacetate to produce cyanoacrylate with high yields. The reaction's key characteristics are a shorter reaction time, a large substrate scope, the viability of different functional groups, ease of work-up, and high yields, which provide environmental benefits. Furthermore, the anticancer activity of the synthesized series of cyanoacrylate derivatives was tested against A549, HT-29, and HepG2 cell lines.\u0000 \u0000Resumen. El aetato de diisopropiletilamonio (DIPEAc) se empleó como catalizador en la reacción de condensación de Knoevenagel emplenado aldehídos y cianoacetato de etilo para producir cianoacrilatos en reindimientos elevados. Algunas características de esta aplicación es el que requiere tiempos cortos de reacción, se puede practicar en una amplia gama de sustratos, tolera varios grupos funcionales, el trabajo de la reacción es sencillo y tiene elvados rendimientos, lo que es compatible con el medio ambiente. Los productos obtenidos fueron probados con las líneas celulares A549, HT-29 y HepG2.","PeriodicalId":17377,"journal":{"name":"Journal of the Mexican Chemical Society","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89287825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the chemical composition and biological activities of essential oils from two Satureja species: Molecular docking studies","authors":"Behrouz Ezatpour, N. Dorosti, E. Rezaee, F. Ghaziani","doi":"10.29356/jmcs.v67i1.1816","DOIUrl":"https://doi.org/10.29356/jmcs.v67i1.1816","url":null,"abstract":"The Satureja species (family Lamiaceae) are economically important plants; they have been used as medicinal plants, flavoring in food, and cosmetic material for centuries. The volatile oils of two Satureja species, S. khuzistanica and S. rechingeri, were obtained by hydrodistillation method with Clevenger-type apparatus. The chemical composition of oils was analyzed by gas chromatography coupled with mass spectrometry (GC-MS). The major constituent of S. khuzistanica oil was Carvacrol (68.7%) and those of S. rechingeri oil were Thymol (51.28%) and Carvacrol (22.08%). Anticholinesterase and anticancer activities were screened by Ellman’s method and MTT assay, respectively. Besides, the role of non-covalent interactions in cholinesterase enzyme (ChE) inhibition by the main ingredient, Carvacrol, was studied through docking calculations. The inhibitory activity of S. khuzistanica oil was higher than those of S. rechingeri oil with IC50: 377.14±2.36 and 251.37±1.88 µg/ml against acetylcholinesterase enzyme (AChE) and butyrylcholinesterase enzyme (BChE). S. rechingeri essential oil was found to possess relatively moderate cytotoxic activity with IC50 values of 488.96±3.19 µg/ml and 767.22±3.19 µg/ml on A2780 and PC-3 cells, respectively. The role of hydrogen bonding and π…π stacking interactions in enzyme inhibition by a common ingredient, Carvacrol, was characterized.\u0000 \u0000Resumen. Las especies Satureja (familia Lamiaceae) son plantas económicamente relevantes; durante siglos se han utilizado como plantas medicinales, saborizantes en alimentos y material cosmético. Se obtuvieron los aceites volátiles de dos especies de Satureja, S. khuzistanica y S. rechingeri, empleando el método de hidrodestilación con un aparato tipo Clevenger. La composición química de los aceites se analizó mediante cromatografía de gases acoplada a espectrometría de masas (GC-MS). El componente principal del aceite de S. khuzistanica fue el carvacrol (68,7 %) y los del aceite de S. rechingeri fueron el timol (51,28 %) y el carvacrol (22,08 %). Se evaluó la actividad anticolinesterasa y anticancerígena emplenado el método de Ellman y el ensayo MTT, respectivamente. Además, se estudió el papel de las interacciones no covalentes en la inhibición de la enzima colinesterasa (ChE) por parte del ingrediente principal, Carvacrol, mediante cálculos de acoplamiento. La actividad inhibidora del aceite de S. khuzistanica fue superior a la del aceite de S. rechingeri con IC50: 377,14±2,36 y 251,37±1,88 µg/ml frente a la enzima acetilcolinesterasa (AChE) y la enzima butirilcolinesterasa (BChE). Se encontró que el aceite esencial de S. rechingeri posee una actividad citotóxica relativamente moderada con valores IC50 de 488,96±3,19 µg/ml y 767,22±3,19 µg/ml en células A2780 y PC-3, respectivamente. Se caracterizó el papel de los enlaces de hidrógeno y las interacciones de apilamiento π…π en la inhibición enzimática por el Carvacrol.","PeriodicalId":17377,"journal":{"name":"Journal of the Mexican Chemical Society","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86695552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alkanes and Chemical Markers Identified in the Essential Oil from Pericarp of Nanfengmiju (Citrus kinokuni Hort. ex Tanaka)","authors":"Jian Wang","doi":"10.29356/jmcs.v67i1.1819","DOIUrl":"https://doi.org/10.29356/jmcs.v67i1.1819","url":null,"abstract":"Based on the previously researches, this manuscript comprehensively analysed the chemicals in essential oil (EO) from pericarp of Nanfengmiju, a variety of Citrus kinokuni Hort. ex Tanaka. The isolated crystals from the EO were mainly composed of a series of alkanes. In total, 33 alkanes were identified, in which 14 ones were firstly reported in the peels EOs from Citrus L., to the best of my knowledge. Previously, alkanes were neglected and never thought as the chemical markers of peels EOs from Citrus. In fact, some of them can be chosen as the markers such as tricosane and pentacasane. Eight compounds including limonene, γ-terpinene, α-terpineol, α-farnesene, linalool, thymol, α-sinensal, and methyl-N-methyl anthranilate had already been identified as the markers of peels EOs from Citrus reticulata Blanco. α-Sinensal and methyl-N-methyl anthranilate were undetected in this study. At the same time, another 2 compounds β-terpineol and δ-cadinene were first selected, and 2 compounds such as spathulenol and isospathulenol were identified previously as the markers of peel EO from Nanfengmiju. In total, twelve markers were identified for peels EO from Nanfengmiju.\u0000 \u0000Resumen. Con base en investigaciones previas, en este trabajo se analizan exhaustivamente los productos químicos presentes en el aceite esencial (EO) del pericarpio de Nanfengmiju, una variedad de Citrus kinokuni Hort. ex Tanaka. Los cristales aislados del EO estaban compuestos principalmente por una serie de alcanos. En total, se identificaron 33, entre los cuales, de acuerdo con lo que sabemos, 14 se informaron por primera vez en los aceites esenciales de cáscaras de Citrus L. En este trabajo se proponen algunos alcanos como marcadores químicos de los aceites esenciales de las cáscaras de cítricos, como el tricosano y el pentacasano. Ocho compuestos, incluidos limoneno, γ-terpineno, α-terpineol, α-farneseno, linalol, timol, α-sinensal y antranilato de metil-N-metilo, ya se han identificado como marcadores de AE de cáscaras de Citrus reticulata Blanco. En este estudio no se detectaron ni el α-sinensal ni el antranilato de metil-N-metilo. Al mismo tiempo, se seleccionaron otros 2 nuevos marcadores: β-terpineol y δ-cadineno, y compuestos como el espatulenol y el isospatulenol se identificaron previamente como marcadores del EO de la cáscara de Nanfengmiju. En total, se identificaron doce marcadores para EO de Nanfengmiju.","PeriodicalId":17377,"journal":{"name":"Journal of the Mexican Chemical Society","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83477563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinetic Study of Ru(III) Promoted Oxidation of L-Tryptophan in an Anionic Surfactant Medium by Hexacyanoferrate(III)","authors":"A. Srivastava, Manjusha, N. Srivastava, R. Naik","doi":"10.29356/jmcs.v67i1.1829","DOIUrl":"https://doi.org/10.29356/jmcs.v67i1.1829","url":null,"abstract":"The kinetic investigation of Ru(III) promoted oxidation of L-tryptophan (Trp) by [Fe(CN)6]3- has been performed in anionic sodium dodecyl sulfate (SDS) micellar medium by recording the decrease in absorbance at 420 nm, corresponding to [Fe(CN)6]3- using UV-visible spectrophotometer. Pseudo-first-order condition has been used to examine the progress of reaction as a function of [Fe(CN)63−], ionic strength, [OH-], [SDS], [Ru3+], [Trp], and temperature by changing one variable at a time. The results exhibit that [OH-], [SDS], and [Trp] are the decisive parameter showing an appreciable effect on reaction rate. The reaction exhibits first-order kinetics in the studies concentration range of Ru(III), [Fe(CN)6]3− and at lower [Trp] and [OH-]. The incremental trend observed in the reaction rate with electrolyte concentration shows a positive salt effect. The reaction rate is almost ten times faster in SDS micellar medium compared to the aqueous medium. [Fe(CN)6]3- does not show any appreciable effect on the critical micellar concentration (CMC) of SDS as the polar head of SDS and [Fe(CN)6]3- both are negatively charged. The K+ obtained from K3[Fe(CN)6] and KNO3 decreases the repulsion between the negatively charged heads of the surfactant molecules thereby decreasing the CMC of SDS. The activation parameters also support the outer-sphere electron transfer mechanism as proposed by us.\u0000 \u0000Resumen. El estudio cinético de la oxidación de L-tryptofano (Trp) con [Fe(CN)6]3- asistida por Ru(III), se llevó a cabo en un medio micelar de dodecilsulfato de sodio aniónico (SDS) y se monitoreó utilizando espectrometría de UV-visible midiendo la disminución de la absorbancia a 420 nm, correspondiente al [Fe(CN)6]3-. Para examinar el avance de la reaccción se utilizaron condiciones de pseudo-primer orden en función de [Fe(CN)63−], fuerza iónica, [OH-], [SDS], [Ru3+], [Trp], y temperatura, variando siempre una sola una variable. Los resultados indican, que los parametros decisivos que tuvieron un efecto apreciable sobre la velocidad de la reacción son [OH-], [SDS], y [Trp]. La reacción sigue una cinética de primer orden en el rango de concentraciones de estudio de Ru(III), [Fe(CN)6]3− y a bajas concentraciones de [Trp] y [OH-]. La tendencia de incremento de velocidad de la reacción con aumento de la concentración del electrolito muestra un efecto salino positivo. La velocidad de la reacción en el medio micelar de SDS es casi diez veces mayor que en solución acuosa. [Fe(CN)6]3- no muestra ningún efecto appreciable en la concentración crítica micelar (CMC) de SDS debido a que el grupo polar del SDS (SO3-, cabeza) y el [Fe(CN)6]3- tienen ambos carga negativa. Los cationes K+ provenientes del K3[Fe(CN)6] y KNO3 disminuyen la repulsión entre las cabezas con cargas negativas del surfactante, bajando así la CMC del SDS. Los parámetros de activación apoyan también el mecanismo de transferencia de electrones de la esfera exterior propuesto.","PeriodicalId":17377,"journal":{"name":"Journal of the Mexican Chemical Society","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76416911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Were biogenic volatile organic compounds mainly responsible for ozone pollution during the COVID-19 lockdown In Mexico City?","authors":"E. Beristain-Montiel, A. Gavilán-García, Sandra Carime Maldonado-Cabrera","doi":"10.29356/jmcs.v67i1.1840","DOIUrl":"https://doi.org/10.29356/jmcs.v67i1.1840","url":null,"abstract":"During the COVID-19 lockdown in Mexico City (March 23 to May 20, 2020), criteria air pollutants decreased their concentration from -19 % (PM10) to -54 % (NO), except for ozone which increased its concentration by +6 % in comparison with the same period of 2019. After the lockdown, the concentration of criteria air pollutants returned to levels before COVID-19, while ozone remained unchanged. As meteorological variables did not modify their trends from 2017 to 2021, all differences in concentration during the lockdown were linked to changes in human activities. The spatial trends during the COVID-19 lockdown were similar to previous years, where the north and center of Mexico City were the most polluted sites, except for ozone which had the highest concentrations in the south and west of the city. During the lockdown, O3 concentration was related to high wind speeds in the direction of green areas, which was attributed to O3 formation from the reaction between biogenic and domestic volatile organic compounds with local NOx emissions. Besides, we did not find evidence that criteria air pollutants were related to COVID-19 spreading. Significant correlations were found only between the number of infections and nitrogen oxides, which may reflect that infected people mobility transmitted the virus in both external and internal spaces. We encourage authorities to pay more attention to other sources besides vehicular for regulation as we demonstrated that although human activities were restricted, pollution could remain at high levels in focused sources.\u0000 \u0000Resumen. Durante el confinamiento por COVID-19 en la Ciudad de México (23 de marzo al 20 de mayo de 2020), los contaminantes atmosféricos criterio disminuyeron su concentración de -19 % (PM10) a -54 % (NO), excepto el ozono que aumentó su concentración +6 %. en comparación con el mismo período de 2019. Después del confinamiento, la concentración de contaminantes atmosféricos criterio volvió a los niveles anteriores al COVID-19, mientras que el ozono se mantuvo sin cambios. Como las variables meteorológicas no modificaron sus tendencias de 2017 a 2021, todas las diferencias de concentración durante el confinamiento estuvieron vinculadas a cambios en las actividades humanas. El análisis espacial mostró tendencias similares a años previos donde el norte y centro de la Ciudad de México fueron los sitios más contaminados excepto ozono, el cual tuvo las concentraciones más elevadas en el sur y oeste de la ciudad. Durante el confinamiento, la concentración de O3 estuvo relacionada con las altas velocidades del viento en dirección a las áreas verdes, lo que se atribuyó a la formación de O3 a partir de la reacción entre los compuestos orgánicos volátiles biogénicos y domésticos y las emisiones locales de NOx. Además, no se encontró evidencia de que los contaminantes atmosféricos criterio estuvieran relacionados con la propagación de COVID-19, ya que solo se encontraron correlaciones significativas entre el núme","PeriodicalId":17377,"journal":{"name":"Journal of the Mexican Chemical Society","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89266058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of the p53 Gene Mutation Using an Ultra-sensitive and Highly Selective Electrochemical DNA Biosensor","authors":"Luis Fernando Garcia-Melo, Norma Andrea Chagoya Pio, M. Morales-Rodríguez, E. Madrigal-Bujaidar, E. Madrigal-Santillán, I. Álvarez-González, R. N. Pineda Cruces, N. Batina","doi":"10.29356/jmcs.v67i1.1880","DOIUrl":"https://doi.org/10.29356/jmcs.v67i1.1880","url":null,"abstract":"Abstract. The p53 gene— “the guardian of the genome”—is responsible for maintaining the integrity of the genome, along with cell cycle regulation, apoptosis, and cell differentiation. New analytical devices are needed to recognize the main alterations this gene could suffer, since it is one of the most frequent in human cancer. For this reason, we developed an electrochemical DNA biosensor with high sensitivity and specificity to monitor the 175p2 mutation of the p53 gene. We modified a screen-printed gold electrode (SPGE) by immobilizing a thiolated DNA probe sequence with 11-mercaptoundecanoic acid to detect its complementary sequence through the hybridization reaction. Doxorubicin (Dox) was used to increase the sensitivity of the biosensor, and the entire process was evaluated using the Cyclic Voltammetry (CV) technique. The measurement range of the developed device is from 1 fM to 100 nM of the p53 gene mutation with a limit of detection (LOD) of 2.2 fM. In the presence of Dox, the LOD increased up to 175 aM, becoming one of the highest efficiency devices in the field. The electrochemical DNA biosensor selectively detects the p53 suppressor gene mutation; it distinguishes between different non-complementary and complementary sequences. Our results indicate a high potential of our sensor for the p53 gene 175p2 mutation detection, which is convenient in the early diagnosis of diseases related to this gene.\u0000 \u0000Resumen. El gen p53—“guardián del genoma”—es responsable de mantener la integridad del genoma, así como de la regulación del ciclo celular, la apoptosis, y la diferenciación celular. Es necesario desarrollar nuevos dispositivos analíticos para reconocer las principales alteraciones que este gen podría sufrir, ya que es uno de los más frecuentes en el cáncer humano. En este sentido, se desarrolló un sensor electroquímico de ADN de alta sensibilidad y especificidad para identificar la mutación 175p2 del gen p53. Para ello, se formó una monocapa sobre un electrodo de oro que contenía secuencias sonda de ADN tiolado junto con ácido 11-mercaptoundecanoico, las cuales se emplearon para detectar la mutación del gen a través de la reacción de hibridación. Finalmente, se utilizó doxorrubicina (Dox) para aumentar la sensibilidad del biosensor; el proceso se evaluó mediante la técnica de Voltamperometría Cíclica (VC). El rango de medición del dispositivo desarrollado es de 1 fM a 100 nM de la mutación del gen p53 con un límite de detección (LOD) de 2.2 fM. En presencia de Dox, el LOD aumentó hasta 175 aM, convirtiéndose en uno de los dispositivos de mayor eficiencia en el campo. El biosensor electroquímico de ADN detecta selectivamente la mutación del gen supresor p53 y es capaz de distinguir entre diferentes secuencias complementarias y no complementarias. Nuestros resultados indican un alto potencial del biosensor para la detección de la mutación 175p2 del gen p53, lo cual es conveniente en el diagnóstico oportuno de enfermedades relacionadas con es","PeriodicalId":17377,"journal":{"name":"Journal of the Mexican Chemical Society","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85083961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}