Screening Method Synthesis of AgNPs Using Petroselinum crispum (parsley) Leaf: Spectral Analysis of the Particles and Antibacterial Study

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
S. Alshahrani, A. Alameri, R. Zabibah, A. Jalil, Omid Ahmadi, G. Behbudi
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引用次数: 1

Abstract

Abstract. The synthesis and applications of nanomaterials are an excitingand emerging field of scientific research. In this paper, silver nanoparticles (AgNPs) were synthesized using parsley leaf extract via the screening method (autoclave hydrothermal heating, and heater), and their microbial activities were studied. An extract of chopped parsley leaves was prepared. AgNO3 salt with a concentration of 1 mM was used. In every two methods of autoclave hydrothermal heating and heater, 5 mL of the Parsley leaf extract was mixed with 25 mL of silver nitrate salt. By FTIR analysis, the presence of Petroselinum crispum (parsley) leaf extract in NPs was identified. By using a hydrothermal autoclave and heater to synthesize AgNPs at the lowest wavelength, the size of NPs was achieved at 403 nm and 414 nm, respectively. All samples synthesized by different techniques had suitable zeta potential and acceptable stability. By giving the zeta potential of NPs synthesized by autoclave (-14.3 mV), results showed that this method is suitable for the production of AgNPs. Comparing the antibacterial attributes of the synthesized Ag NPs by different techniques (autoclave and heater) on Gram-negative bacteria (E. coli) showed 16±1, and 12±1, respectively.   Resumen. La síntesis y las aplicaciones de los nanomateriales son un campo de investigación científica emocionante y emergente. En esta investigación, se sintetizaron nanopartículas de plata (AgNP) utilizando extracto de hoja de perejil a través del método de selección (calentamiento hidrotermal en autoclave y calentador), y se estudiaron sus actividades microbianas. Se preparó un extracto de hojas de perejil picadas. Se utilizó AgNO3 a una concentración de 1 mM y se utilizaron los métodos de calentamiento en autoclave y calentador; se mezclaron 5 mL del extracto de hoja de Perejil con 25 mL de sal de nitrato de plata. Mediante análisis FTIR se identificó la presencia de extracto de hoja de Petroselinum crispum (perejil) en NPs. El tamaño de las NP se logró a 403 nm y 414 nm, con los métodos de calentamiento en autoclave y calentador, respectivamente. Todas las muestras sintetizadas por diferentes técnicas tuvieron un potencial zeta adecuado y una estabilidad aceptable. Al dar el potencial zeta de las NP sintetizadas en autoclave (-14,3 mV), los resultados mostraron que este método es adecuado para la producción de AgNP. La comparación de los atributos antibacterianos de las AgNP sintetizadas por diferentes técnicas (autoclave y calentador) en bacterias Gram-negativas (Escherichia coli) dieron 16±1 y 12±1, respectivamente.
用欧芹叶合成AgNPs的筛选方法:颗粒的光谱分析及抗菌研究
摘要纳米材料的合成和应用是一个令人兴奋的新兴科学研究领域。本文以欧芹叶提取物为原料,通过筛选法(热压釜热液加热和加热器加热)合成了纳米银,并对其微生物活性进行了研究。制备了切碎的欧芹叶提取物。选用浓度为1mm的AgNO3盐。在热压罐热液加热和加热器两种方法中,将5ml欧芹叶提取物与25ml硝酸银盐混合。通过红外光谱(FTIR)分析,鉴定出NPs中含有欧芹叶提取物。利用热液热压罐和加热炉在最低波长合成AgNPs,分别在403 nm和414 nm处获得了纳米颗粒的尺寸。不同工艺合成的样品均具有合适的zeta电位和可接受的稳定性。通过对高压灭菌法合成的纳米粒子的zeta电位(-14.3 mV)测定,结果表明该方法适用于AgNPs的制备。比较不同工艺(高压灭菌法和加热法)合成的Ag NPs对革兰氏阴性菌(大肠杆菌)的抑菌性能,分别为16±1和12±1。Resumen。纳米材料的应用研究进展:investigación científica emocionante y emergenente。En esta investigacion se sintetizaron nanoparticulas德银(AgNP)使用extracto de hoja de perejil traves del metodo de seleccion (calentamiento hidrotermal En高压釜y calentador), y se estudiaron sus对于microbianas。见preparó unextracto de hojas de perejil picadas。Se utilizó AgNO3 a una concentración de 1 mM / mM / mM / mM / mM / mM / mM / mM / mM / mM / mM / mM / mM用mezclaron 5 mL de hoja de Perejil萃取物和25 mL de nitrode plata。Mediante análisis FTIR se identificó la presencia de extracto de hoja de Petroselinum crispumm (perejil) en NPs。El tamaño de las NP se logró a 403 nm和a 414 nm,分别采用高压灭菌法和热压灭菌法对其进行了改进。今天的研究表明,不同的人的生活方式不同,他们的生活方式不同,潜在的生活方式也不同,这是不可接受的。在高压灭菌器(- 143,3 mV)中,使用Al - dar电位zeta de las NP,其结果为mostraron que este m todo es adecuado para la producción de AgNP。La comparación de los atributos antibacterios de las AgNP sinintetizas与 δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ δ。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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