Veterinary science, technologies of animal husbandry and nature management最新文献

{"title":"Therapeutic efficiency of antigelminth preparations at skrjabinemosis of sheep","authors":"V. Melnychuk","doi":"10.31890/vttp.2019.04.23","DOIUrl":"https://doi.org/10.31890/vttp.2019.04.23","url":null,"abstract":"The intensive and sometimes uncontrolled use of anthelmintic agents to control the nematodoses of the digestive tract of animals has led to the emergence of resistant helminth strains, which has become an important problem for scientists in many European countries, including Ukraine. Thus, the drugs most commonly used to treat animals are not effective enough against the agents of sheep helminthiasis. In this case, the question of the actual effectiveness of anthelmintics in relation to specific pathogens remains important for both scientists and veterinary practitioners. The aim of the study was to study the therapeutic efficacy of anthelmintics, registered on the territory of Ukraine which belong to different chemical groups for skrjabinemosis sheep. Experimental testing of modern anthelmintic preparations was carried out: Brovalsen powder, Albendazole-250 tablets and Albendazole suspension 10 % (benzimidazole group); Brovalevamisole powder 8 % and Levavet injection solution 10 % (imidotiazole group); Univerm powder and Ivermekvet injection solution 1 % (macrolide group); Kombitrem emulsion and Kloziveron injection solution (combination group). Studies have shown that all the tools used in the experiments have an anti-helminthic effect against the pathogen of sheep skrjabinemosis (EE – 70–100 %, IE – 77.49–100 %). The most effective were the chemicals from the group of combination preparations (Kombitrem and Kloziveron)in which EE and IE reached 100 %. The effectiveness of drugs from the group of macrocyclic lactones depended on the method of their setting. The use of Ivermekvet injection solution 1 % and the individual feeding of the Univerm powder resulted in 100 % efficiency of the agents. The group feeding of the Univerm powder proved to be less effective (EE – 90 %, EE – 92.54 %). A similar effect has been reported for the use of preparations of the imidotiazole group. Injection of Ivermekvet 1 % and individual feeding of Brovalevamisole 8 % showed 100 % effectiveness. Group administration of Brovalevamisole 8 % led to a decrease in performance (EE – 80 %, IE – 86.99 %). It was determined that the use of various methods of benzimidazole group (individual or group) did not destroy 100 % of the pathogens of sheep skrjabinemosis in the body of sheep. The EE and IE ratios of Brovalsen powder, Albendazole-250 tablets and Albendazole suspension 10 % ranged from 70–90 % and 77.49–95.70 %, respectively.","PeriodicalId":173736,"journal":{"name":"Veterinary science, technologies of animal husbandry and nature management","volume":"120 1-3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114026134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The analysis of canine parvovirus strains based on the sequence VP-2 GENE","authors":"P. Yurko, O. Shcherbak, V. Borovkova, I. P. Danilov","doi":"10.31890/vttp.2019.04.33","DOIUrl":"https://doi.org/10.31890/vttp.2019.04.33","url":null,"abstract":"One of the most dangerous diseases of dogs is canine parvovirus enteritis. The causative agent of enteritis is Canine Parvovirus type 2 (CPV-2) of the subgroup Feline panleukopenia virus (FPV) of the species Carnivore protoparvovirus 1 of the family Parvoviridae. For prevention both in the world and in Ukraine vaccination is used. Despite this measure, parvovirus enteritis still causes death of dogs with a clinical and pathological pattern characteristic of the disease. The sequence of dog parvovirus strains was analyzed, which are presented in the GenBank database: No. MH213140.1, KP881653.1, GU212792.1, GU212790.1, FJ011098.1, GU212791.1, MG264079.1, GU392239.1, KF539801.1, MH491947.1, MH491855.1, DQ025952.1, MH329287.1, AY262281.1, KY921606.1. Among the given strains, 5 (GU212792.1, GU212790.1, FJ011098.1, GU212791.1, KY921606.1) are used for the manufacture of vaccines registered in Ukraine. Pathogenic isolates were isolated in Italy, Hungary, France, Taiwan, China, South Korea, etc. As a result of the studies, SNPs (Single Nucleotide Polymorphism) were revealed in the sequences and studied, and a phylogenetic tree was constructed (method of attachment of neighbors). The smallest distances were established between the vaccine strains VAC_M primodog (GU212790.1), VAC_P vanguard (GU212791.1), VAC_C quantum (GU212792.1). Intervet vaccine strains (FJ011098.1) and MX-VACVBC / 17 (KY921606.1) are in a separate cluster from the other three vaccine strains. They form a separate cluster. Also, the closest distances are established between the isolates according to the territorial distribution. The results obtained may indicate differences in the antigenic structure of circulating pathogenic strains of dog parvovirus. The lack of data from epizootological monitoring of the disease using molecular genetic methods in Ukraine shows the need for such studies.","PeriodicalId":173736,"journal":{"name":"Veterinary science, technologies of animal husbandry and nature management","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130328316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution and principles of treatment of cats ehrlichiosis in the conditions of the metropolis of Kharkov","authors":"I. Yevtushenko, E. S. Makarova","doi":"10.31890/vttp.2019.04.11","DOIUrl":"https://doi.org/10.31890/vttp.2019.04.11","url":null,"abstract":"The article presents data on the distribution of ehrlichiosis among cats in the metropolitan city of Kharkiv and the basic principles of its treatment. Recently, the number of cases of domestic pets with transmissive diseases has been increasing in Ukraine. One of these is the naturally occurring, transmissive diseases of cats and dogs caused by rickettsiae of the genus Ehrlichia, known as ehrlichiosis. In recent years, there has been an increasing number of publications on the incidence of cats with ehrlichiosis. \u0000Ehrlichia are gram-negative, obligate, intracellular agents. Their average size is 0,5-1,5 microns. Plasma monocytes are parasitize in the form of morules, which are colored blue according to Romanovsky-Giemsa. Ixodidae ticks, primarily Rhipicephalus sanguineus, transmit the pathogen agents of this disease. \u0000As a result of the studies, it was found that ehrlichiosis was registered in 20,6 % of the examined animals of different ages, sex and breed. In cats aged 2-5 years old, the disease has registered more often. It should be noted that 11,26 % of animals were found to be infected with several pathogens of vector-borne diseases (babesiosis and ehrlichiosis). Monitoring of clinical symptoms in cats with ehrlichiosis was characterized by specific clinical symptoms: increase in body temperature to 39,8-41,0 °C, apathy, anorexia, periodic vomiting. In some cases, paleness or jaundice of the mucous membranes, hemoglobinuria, nasal bleeding, limping, and weakness of the hind limbs were registered. For the diagnosis, direct microscopy of peripheral blood smears was performed following the procedure using a Leukodif 200 blood smear rapid stain kit. To confirm the diagnosis, the material was examined for the presence of the pathogen DNA in the polymerase chain reaction of LLC \"AGROGEN NOVO\" Kharkov. The strategy of treating cat ehrlichiosis included the complex application of modern medicines (antibacterials, immunostimulants, metabolic correctors, hepatoprotectors, probiotics). This treatment scheme allows to achieve complete elimination of the pathogen from the animal.","PeriodicalId":173736,"journal":{"name":"Veterinary science, technologies of animal husbandry and nature management","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126319050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of nervous processes on calcium-phosphorus ratio in blood of cows in different seasons","authors":"O. Zhurenko","doi":"10.31890/vttp.2019.04.14","DOIUrl":"https://doi.org/10.31890/vttp.2019.04.14","url":null,"abstract":"In animals with different types of higher nervous activity (HNA), the ratio of total calcium to inorganic phosphorus in blood serum stayed within the physiological limits and was 1.6–2.0 r. u. Despite of the season, the index in cows of weak type of HNA was 6.3–13.3% (p <0.05) higher than in SBM type of cows. Compared to cows with SBM, SBI and SU type of HNA the total calcium to inorganic phosphorus ratio in blood serum was not significantly different regardless of season. In cows with SU and a weak type of HNA, in winter this index in the serum of blood was higher by 17.3% (p<0.001) and 14.9% (p<0.05) than in warm season. Whereas in cows with balanced types of HNA (SBM and SBI), only the corresponding trend was observed in the range of 8–13%. Therefore, the strength of nervous processes more significantly limited the calcium-phosphorus ratio in the blood serum of cows in summer, whereas the balance – in cold season. Movement of nervous processes in cows does not significantly influence on the calcium-phosphorus ratio in the serum of cows. It was found that the strength of the nervous processes both in summer and winter was inversely related to the calcium-phosphorus ratio in the blood (r = -0.51–0.58; p<0.05-0.01). Nervous process balance is inversely related to the calcium-phosphorus ratio in winter (r = -0.52; p<0.05), whereas in summer this is insignificant (r = -0.29). Movement of nerve processes, despite of the season, is not connected with the indicator of calcium-phosphorus ratio in the blood of cows (r=-0.08–0.29). \u0000The formation of the calcium-phosphorus ratio in the blood of cows and main characteristics of nervous processes is determined by regression analysis. The coefficient of determination of the strength of nerve processes to the calcium-phosphorus ratio in whole blood of cows indicates that in summer up to 26% (p<0.05) and in winter up to 33% (p<0.05) variations can be determined by the strength of the nervous processes. \u0000Therefore, accurate connectivity and substantial influence of the main characteristics of cortical nerve processes and seasons on the calcium and phosphorus content in the blood of cows have been confirmed.","PeriodicalId":173736,"journal":{"name":"Veterinary science, technologies of animal husbandry and nature management","volume":"148 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116544272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics of some indexes of lipids metabolism between ewes of the east of Ukraine","authors":"P. Sharandak, O. Tymoshenko, G. Vikulina, D. Kibkalo, S. B. Borovkov","doi":"10.31890/vttp.2019.04.32","DOIUrl":"https://doi.org/10.31890/vttp.2019.04.32","url":null,"abstract":"Lipids' metabolism in sheep plays a great role, because it is the main part of the wool. Estimation of cholesterol content and its fractions in blood serum is the important aspect of dispensary system in sheep. The aim of the work is to study the state of lipids’ metabolism in sheep on the example of animals that were kept in the Lugansk region. \u0000The work was carried out on ewes of the Romanov breed in 2008-2014 based on the Lugansk National Agrarian University and farms of Lugansk Region: private of Krasnodon, NNVAK “Kolos” Lutugino, \"Agrofirm \" Aidar of Markivka, Agrofirm “Privillya” of Troitsk, PJSC \"Tribal Plant named after Litvinov\" of Slovyanoserbsk districts. The studies were carried out on ewes of different physiological status – in-lamb (n=19), lactating (n=19) and yield (n=7). Animals were kept in brick premises during the period of gestation and lactation. Yield ewes were in grazing hold. \u0000It was established that rations of ewes of Lutuginsky, Markovsky and Troitsky districts had an excessive proportion of roughage (74,7–85,2 %), Krasnodonsky and Slavyanoserbsky - concentrated (42,7 and 42,4 %). Juicy feed (corn silage) was only in the ration of ewes of the Markivka district, but it contained only a few concentrates (9,0 %). In addition, the following violations were found: a) excess of dry matter (2,37–3,0 kg at normal – 1,6 in in-lamb and 2,3 in lactating ewes) in combination with a lower concentration in 1 kg of dry matter of exchange energy (7,75–9,94 mJ for the norm of 10,3 and 10,5 mJ respectively), raw and digestible protein (48,1–84,9 g and 46,9–82,8 g of demand respectively), sugar (1,55–3,7 g, if necessary, 6,5–7,5), starch (1,95–17,7 g; norm – 20–21), phosphorus (1,4–2,66 g, norm 3,6–4,0), sulfur (1,36–2,36 g; norm – 2,6–2,7), copper (3,52–8,3 mg, norm – 8,8–9,0 mg), zinc (20,5–26,2 mg; norm – 34–36), manganese (23,4–45,9 mg, norm – 48–50) and iodine (0,09–0,35 mg rate – 0,4–0,5); b) a low relation between light-fermented carbohydrates (starch and sugar) and digestible protein (0,39–0,45; the norm is 0,7–0,8), which could cause disorder of rumen metabolism and development of liver pathology; c) violation of the ratio between calcium and phosphorus (2,25–5,9: 1, the norm is 1,5–2: 1). \u0000By a clinical study of ewes of three physiological groups (in-lamb, lactating, yield) no changes were found. In 45 animals which we have been examined, lipids’ metabolism was analysed using biochemical methods. The highest concentration of total cholesterol was in the blood serum of yield ewes – 1,53±0,14 mmol/l (0,87–1,82). Nevertheless, we have not established the probable difference (p<0,5) in the content of cholesterol in ewes of different groups. By calculating the mean square deviation (n=45, 2δ = ±0,75) it was found that 97,9 % of all values were in the range from 0,65 to 2,15 mmol/l. \u0000The content of triacylglycerols in the blood serum of clinically healthy in-lamb ewes varied significantly from 0,27 to 1,18 mmol/l. The smallest content ","PeriodicalId":173736,"journal":{"name":"Veterinary science, technologies of animal husbandry and nature management","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127912755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinically-hematological, biochemical and imunological figures dinamic of clinically healthy animals during the action of anthyhomotoxyc preparation Traumel","authors":"V. Kushnir, M. Todorov","doi":"10.31890/vttp.2019.04.20","DOIUrl":"https://doi.org/10.31890/vttp.2019.04.20","url":null,"abstract":"The effect of antihomotoxic prepapation Traumel on the clinical condition, morphological, biochemical and immunological parameters of blood and blood serum of clinically healthy dogs is determined in the article on the basis of experimental studies. \u0000The problem of using antihomotoxyc preparations for the treatment of animal diseases is one of the most controversial issues in modern clinical medicine, but there is growing interest in finding alternative treatments in the world. Today there is a number of research and publications claiming that complex anthyhomotoxyc preparations have a correcting effect. Some scientists talk about the immunostimulatory effect of anthyhomotoxyc preparations. However, all of these studies were conducted mainly in humane medicine and in laboratory animals. Thorough studies of the effects of the anthyhomotoxyc preparation Traumel on the organism of clinically healthy dogs have not been conducted previously. Therefore, this work is relevant and timely. \u0000The purpose of our work is to study the effect of complex anthyhomotoxyc preparation Traumel on clinically healthy animals. \u0000The material for the study was a group of ten clinically healthy dogs, which before the use of anthyhomotoxyc preparation were determined based on the clinical condition, morphological, biochemical and immunological parameters of their blood. Then we injected the preparation. The preparations were given in a dose of 1 ml per animal per day. Blood and blood serum tests were performed on the first, third, sixth and thirtieth days. The results were compared with those obtained during the determination of the state of the organism before the preparation use. \u0000Laboratory studies were conducted at the laboratory of the Odessa Regional Clinical Hospital. \u0000The results of our research show that complex antigomotoxicin preparation Traumel has a moderate effect on the body of clinically healthy dogs, which has no pronounced clinical manifestations. Blood and blood serum levels do not go beyond physiological limits.","PeriodicalId":173736,"journal":{"name":"Veterinary science, technologies of animal husbandry and nature management","volume":"162 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115436547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increasing of stress resistance in the sport horse training system","authors":"I. A. Каbasova","doi":"10.31890/vttp.2019.04.16","DOIUrl":"https://doi.org/10.31890/vttp.2019.04.16","url":null,"abstract":"The studies to improve the training system and preparation of horses for competitions, taking into account the stress stability factor in order to more objective assessment of the working qualities of horses and conducting a better selection in breeding; enhancement of sport results of Ukrainian riders at National and International competitions have been conducted in this work. A set of measures was developed to increase the stress resistance during demonstrations and competitions, aimed at gradually adapting horses to stressors. \u0000For conducting experiments, 10 horses of the jumping group of Dergachi Children and Youth Equestrian Sport School, who had the experience of participating in the competition to overcome obstacles, were selected. Research of the types of HND of horses was carried out according to the methodical recommendations of VNIII horse breeding. Sport performance was assessed by the following indicators: average number of penalty points per start; % of starts without penalty points; % of starts with prize places; % of starts who were the fastest, but with a penalty points; % of disqualification. \u0000We have formed two equivalent groups of horses – control and experimental, with five horses in each, based on the results of the participation of horses in competitions, taking into account the types of higher nervous activity and sex-age indicators. A set of measures for increasing stress resistance was introduced into the training system of horses of the experimental group from the beginning of the preparatory period in December 2016. \u0000Studies have shown that the most advanced in show jumping are horses with a strong balanced mobile higher nervous activity. Horses of a strong balanced inert type of higher nervous activity are suitable for competing with beginners and as hobby-class horses. Horses of a strong unbalanced type of higher nervous activity have an untapped athletic potential, but they need a training system designed not only to develop their physical qualities but also to increase their stress resistance in competition. \u0000The results of the conducted researches allow to recommend the application of the developed complex of measures in the system of training horses of a strong balanced inert and severe unbalanced types of higher nervous activity; that will allow the best selection of the best animals in breeding by the main selection criterion – ability to work and to receive more competitive horses for participation in National and International competitions for classical equestrian sport, which will increase the level of implementation of horses, price indices and the economic efficiency of the work of breeding farms in general.","PeriodicalId":173736,"journal":{"name":"Veterinary science, technologies of animal husbandry and nature management","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133649664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hematological profile of dry cows in different seasons of the year","authors":"L. Koreyba, Y. Duda","doi":"10.31890/vttp.2019.04.17","DOIUrl":"https://doi.org/10.31890/vttp.2019.04.17","url":null,"abstract":"The study of metabolism in cows, depending on the physiological state, conditions of retention and feeding at different seasons of the year is a necessary condition for directed action on their reproductive capacity and productivity. The season of the year significantly affects on the change in the number of erythrocytes, leukocytes, hemoglobin content, hematocrit, and a total blood amount of cows, despite of their breed. These indices are the lowest in the winter. \u0000The goal of the work was to study the hematological profile of dry cows in different seasons of the year. \u0000Materials and methods. Studies were conducted on cows of the Bos taurus Holstein cows with milk productivity of 5–6 thousand kg for lactation during the physiological course of the dry period (day 11 to 53 of gestation) in different seasons of the year. \u0000Hematologic studies were performed according to the following indicators: (erythrocytes, hemoglobin, color index, SSE, leukocytes, monocytes, lymphocytes, rod and segmented neutrophils, basophils, and eosinophils), following conventional methods. \u0000Results of research and discussion. Analysis of hematologic indices shows that the cellular composition of blood is different at each season of the year. A significant decrease in hemoglobin content presented in the spring (117,61 ± 3,63g / l), compared with summer (139,49 ± 4,73g / l, p <0,01), autumn (134,35 ± 4.66 g / l, p <0.01) and winter (132.40 ± 4.95 g / l, p <0.05) in the blood test of dry cows. At the same time, the highest hemoglobin content compared with the low erythrocyte counts increased by 1.33 times (p <0.01), compared to spring. In summer the tendency of increased leukocytes to 10,33 ± 0,46 g / l was observed but remained within the limits of the physiological norm, and in autumn decreased to a minimum - 8,33 ± 0,31g / l. Analysis of the leukocyte formula showed that in summer the highest percentages of lymphocytes (74.67 ± 2.40%) and basophils (2.58 ± 0.33%) were observed compared to other periods of the year. \u0000The content of segmented nuclear neutrophils was increased to 21.10 ± 1.24 % in spring months, to 20.25 ± 1.33 % in autumn, because of decrease of rod - nucleated neutrophils, in spring to 2.70 ± 0.46% and in autumn to 4.30 ± 1.02%, indicating a physiological change of the neutrophil formula to the left during these periods of the year. It is known that the number of eosinophils increases in stressful situations. Thus, a comparative analysis of the number of eosinophils in the blood of animals showed that their largest value in the leukocyte formula was in winter, and during the transition from winter to spring period, a significant decrease of eosinophil was found in 1.62 times (p <0.05). \u0000Conclusions and prospects for further research. Seasonal variations of hematological status of calving cows of Holstein breed were revealed: in winter-spring period hemoglobin decreased to 117.61 ± 3.63 g / l and its growth in summer (139.49 ± 4.73 g / l); in summer months t","PeriodicalId":173736,"journal":{"name":"Veterinary science, technologies of animal husbandry and nature management","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132857865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modelling and treatment of the uveitis in rabbit","authors":"V. Doroshchuk","doi":"10.31890/vttp.2019.04.09","DOIUrl":"https://doi.org/10.31890/vttp.2019.04.09","url":null,"abstract":"Modelling of the toxico-allergic uveitis is better conducted on rabbits. For the treatment, contrary to corticosteroids that generate a number of side effects (e.g. suppression of reaction, immune inhibitions etc.), one can successfully use nonsteroid drugs, particularly Voltaren and Vetofluxin, which render phagocytes stimulation and immunoglobulin control. As a result, use of these drugs brings to recovery within 7 days. \u0000The decrease in the level of immunoglobulins in the blood serum during the treatment of uveitis compared with levels untreated animals was accompanied by a sharp attenuation of the inflammatory response in the blood test and taking into account the features of immunological protection of the eye (the presence of blood-ocular barrier between blood and chamber moisture, vascular membrane, retina, vitreous body) it should be emphasized that the body as a whole responds to the inflammatory process in the uveal tract. \u0000Considering toxico-allergic uveitis as an autoimmune disease and in respect that the positive effects of the drugs used on inflammatory and allergic processes, we can predict that the drugs we use are able to participate in the regulation of antibody-forming function of B-cells and inhibite autoimmune disorders. \u0000Both treatment schemes show the stimulating effect on phagocytosis, comparing with using phytohemagglutinin, which show no effect. In experimental toxico-allergic uveitis, the treatment regimen in the first experimental group not only did not reduce, but moreover, retained the potential effect on the phagocytic activity of leukocytes. \u0000Compared with phytohemagglutinin impact on phagocytosis, found that both represented treatment strategies stimulate the phagocytosis. In experimental toxico-allergic uveitis, Voltaren and Vetofluxin not only did not reduce, as is usually the case with corticosteroids, but moreover, clearly showed a stimulating effect on the phagocytic activity of peritoneal macrophages. \u0000Thus, studies have shown that in the treatment of such a severe inflammatory process as uveitis, in contrast to corticosteroids, the use of which is accompanied by a number of undesirable effects (inhibition of regeneration, inhibition of immune reactions, etc.), you can successfully use non-steroidal anti-inflammatory drugs, in particular Voltaren and Vetofluxin. The stimulating phagocytosis effect of Voltaren and Vetofluxin and ability to regulate the levels of immunoglobulins during inflammation has been established.","PeriodicalId":173736,"journal":{"name":"Veterinary science, technologies of animal husbandry and nature management","volume":"86 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124747337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Temperature on the lytic activity of Bacteriophage PHAGE SAVB14, specific for STAPHYLOCOCCUS AUREUS VARIANT BOVIS","authors":"Y. Horiuk, M. Kukhtyn, V. Horiuk, V. Mizyk","doi":"10.31890/vttp.2019.04.07","DOIUrl":"https://doi.org/10.31890/vttp.2019.04.07","url":null,"abstract":"Therapeutic effect in case of phagotherapy is determined by the degree of lytic activity of bacteriophages in relation to contagions. Various factors influence the lytic activity of phages including temperature, pH, shelf-life, etc. Temperature plays a considerable role in attachment, penetration, reproduction and duration of latent period. The purpose of this research was to learn the influence of different temperature on lytic activity of bacteriophage Phage SAvB14 which is active in relation to Staphylococcus aureus variant bovis. For the increase of bacteriophage titre Phage SAvB14 the strain of gold Staphylococcus aureus var. bovis 1491f (Certificate for the strain - #736, from 05.03.2019) was used which was abstracted from the sample of the secretion of mammary gland of the patient with subclinical form of mastitis. For determination of temperature influence (0, 4, 8 and 45, 55, 65 °C) aliquots were sown by a double agar in definite intervals by the generally accepted methods. The cups were incubated at temperature 37 °C during 18-24 hours. Research was repeated three times. \u0000According to the results of research it was determined that warming up of test tubes with bacteriophages at temperature 45 oС reduced the activity of phages in 30 minutes of influence in 1,7 times. Temperature 55 and 65 oС had more harmful effect, during the influence for the first 30 minutes the amount of active phages diminished in 2,5 and 5,3 times, and for 60 minutes destroyed the bacteriophage Phage SAvB14 by 89,9 and 93,3% respectively. The obtained results testify the thermolability of Phage SAvB14 which is an important factor in making phage preparation. The most optimum terms for storage of Phage SAvB14 are temperature regime within the limits of 4 and 8 °C. In case of such temperature lytic activity diminished only in 1,5 – 1,7 times, while in case of 0 °C in 2,4 times compared to the initial amount of bacteriophages.","PeriodicalId":173736,"journal":{"name":"Veterinary science, technologies of animal husbandry and nature management","volume":"703 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115307790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}