温度对金黄色葡萄球菌变异BOVIS特异性噬菌体SAVB14裂解活性的影响

Y. Horiuk, M. Kukhtyn, V. Horiuk, V. Mizyk
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引用次数: 0

摘要

在吞噬治疗的情况下,治疗效果是由与传染有关的噬菌体的溶解活性程度决定的。影响噬菌体裂解活性的因素有温度、pH、保质期等。温度在附着、渗透、繁殖和潜伏期等方面起着重要的作用。本研究的目的是了解不同温度对与金黄色葡萄球菌变异牛相关的噬菌体噬菌体SAvB14裂解活性的影响。为了提高噬菌体滴度,使用从亚临床乳腺炎患者乳腺分泌物样本中提取的金黄色葡萄球菌变种牛1491f(菌株证书- #736,2019年3月5日)。为了确定温度的影响(0、4、8和45、55、65°C),按普遍接受的方法,在确定的间隔内用双琼脂播种等分。37℃孵育18-24小时。研究重复了三次。根据研究结果,在45°oС温度下用噬菌体加热试管,在30分钟的影响下,噬菌体的活性降低了1.7倍。温度55和65 oС的影响更大,在前30分钟的影响中,活性噬菌体的数量分别减少了2,5和5,3倍,在60分钟的影响中,噬菌体噬菌体SAvB14的破坏率分别为89,9和93.3%。所得结果证明了噬菌体SAvB14的耐热性是制备噬菌体的重要因素。噬菌体SAvB14的最佳贮藏条件是温度在4℃和8℃范围内。在此温度下,与初始噬菌体数量相比,裂解活性仅下降1,5 - 1,7倍,而在0℃情况下,裂解活性下降2,4倍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Temperature on the lytic activity of Bacteriophage PHAGE SAVB14, specific for STAPHYLOCOCCUS AUREUS VARIANT BOVIS
Therapeutic effect in case of phagotherapy is determined by the degree of lytic activity of bacteriophages in relation to contagions. Various factors influence the lytic activity of phages including temperature, pH, shelf-life, etc. Temperature plays a considerable role in attachment, penetration, reproduction and duration of latent period. The purpose of this research was to learn the influence of different temperature on lytic activity of bacteriophage Phage SAvB14 which is active in relation to Staphylococcus aureus variant bovis. For the increase of bacteriophage titre Phage SAvB14 the strain of gold Staphylococcus aureus var. bovis 1491f (Certificate for the strain - #736, from 05.03.2019) was used which was abstracted from the sample of the secretion of mammary gland of the patient with subclinical form of mastitis. For determination of temperature influence (0, 4, 8 and 45, 55, 65 °C) aliquots were sown by a double agar in definite intervals by the generally accepted methods. The cups were incubated at temperature 37 °C during 18-24 hours. Research was repeated three times. According to the results of research it was determined that warming up of test tubes with bacteriophages at temperature 45 oС reduced the activity of phages in 30 minutes of influence in 1,7 times. Temperature 55 and 65 oС had more harmful effect, during the influence for the first 30 minutes the amount of active phages diminished in 2,5 and 5,3 times, and for 60 minutes destroyed the bacteriophage Phage SAvB14 by 89,9 and 93,3% respectively. The obtained results testify the thermolability of Phage SAvB14 which is an important factor in making phage preparation. The most optimum terms for storage of Phage SAvB14 are temperature regime within the limits of 4 and 8 °C. In case of such temperature lytic activity diminished only in 1,5 – 1,7 times, while in case of 0 °C in 2,4 times compared to the initial amount of bacteriophages.
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