Journal of the National Medical Association最新文献

{"title":"Disproportionate Racial Impact against Hepatic Cancer Patients: NIS 2020","authors":"Narathorn Kulthamrongsri MD,&nbsp;Kanthajan Tatchaya MD,&nbsp;Kulthamrongsri Narathorn MD,&nbsp;Prasitsumrit Vitchapong MD","doi":"10.1016/j.jnma.2024.07.055","DOIUrl":"10.1016/j.jnma.2024.07.055","url":null,"abstract":"<div><h3>Introduction</h3><p>Hepatobiliary cancer is the second leading cause of cancer-related death worldwide. Prior data showed mixed results against racial impact in this population group, with limited specific results regarding clinical outcomes. We aim to utilize a large national database to provide comprehensive data on the clinical outcomes of hepatobiliary cancer about race.</p></div><div><h3>Method</h3><p>The 2020 National Inpatient Sample was utilized in this study. patients with hepatobiliary cancer were selected by ICD-10 CM code. Inpatient mortality and associated complications, including acute kidney injury (AKI), heart failure, and mechanical ventilation use were analyzed. Logistic regression analysis was used to assess the association between race and complications.</p></div><div><h3>Results</h3><p>We surveyed 20,670 hospitalizations with hepatobiliary cancer. Caucasians accounted for 60.7%, whereas African Americans accounted for 12%. The mean age was 65.38 years, with 66% being male. African Americans had a significantly higher inpatient mortality rate (aOR 1.61, 95%CI (1.10, 2.35), p &lt;0.005) as well as higher odds of acute kidney injury (aOR 1.22; 95%CI (1.10-1.35), p&lt;0.005) compared to Caucasians. We found increased, but not statistically significant in other clinical outcomes compared to Caucasians.</p></div><div><h3>Conclusion</h3><p>African Americans are at a higher risk of experiencing worsening clinical outcomes and mortality rates. Future longitudinal studies could shed light on these long-term connections.</p></div>","PeriodicalId":17369,"journal":{"name":"Journal of the National Medical Association","volume":"116 4","pages":"Page 435"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Case-Based Social Determinants of Health Curriculum for Graduate Medical Education","authors":"Brittany C Flemming MD,&nbsp;Duane D. Kim MD,&nbsp;Sean P. Meagher MD,&nbsp;Dimas C. Espinola MD","doi":"10.1016/j.jnma.2024.07.033","DOIUrl":"10.1016/j.jnma.2024.07.033","url":null,"abstract":"<div><h3>Introduction</h3><p>Social Determinants of Health (SDOH) are conditions in environments where people live, learn, work, play, worship, and age that affect a wide range of health, functional, and quality-of-life outcomes and risks, contributing to health disparities and inequities. Several agencies endorse the importance of SDOH, but limited data is available on effective teaching tools for incorporating SDOH into Graduate Medical Education (GME). We developed a case-based curriculum to increase comfort of trainees and faculty in identifying, researching, and educating on SDOH, and utilized it to promote and assess commitment to clinical practice change.</p></div><div><h3>Methods</h3><p>Kern's six-step methodology guided development of the curricular framework. A selected resident-faculty pair reviewed a subspecialtyspecific case through the lens of an assigned SDOH domain using evidence-based medicine. The pair created an interactive learning module to discuss the case, review evidence, investigate whether the standard of care addresses the SDOH domain, and provide a relevant clinical toolkit item.</p></div><div><h3>Results</h3><p>Pre-curriculum assessments showed gaps between resident-perceived SDOH education frequency and staff comfort with SDOH education. Qualitative feedback demonstrated that the curriculum improved resident-perceived education and comfort of faculty in educating on subspecialty-specific SDOH. Nearly 50 commitment-to-clinical practice change goals were created.</p></div><div><h3>Conclusion</h3><p>Integrated SDOH instruction in GME is a priority, but residents report insufficient instruction and faculty feel ill-equipped to provide such education. Our case-based SDOH curriculum presents a dynamic, sustainable, and clinically relevant solution to bridge both gaps and a unique opportunity for programs to reimagine SDOH education and foster a cadre of healthcare professionals equipped to deliver SDOH-competent care.</p></div>","PeriodicalId":17369,"journal":{"name":"Journal of the National Medical Association","volume":"116 4","pages":"Page 425"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting MACE Through Systemic Inflammation Response Index: NHANES Based Analysis","authors":"Chutawat Kookanok MD,&nbsp;Methavee Poochanasri MD,&nbsp;Tatchaya Kanthajan MD,&nbsp;Voramol Rochanaroon MD,&nbsp;Sethapong Lertsakulbunlue MD,&nbsp;Irin Jariyayothin MD,&nbsp;Nicha Wareesawetsuwan MD,&nbsp;Vitchapong Prasitsumrit MD,&nbsp;Vichayut Chayapinun MD,&nbsp;Nisha Wanichwecharungruang MD,&nbsp;Tulaton Sodsri MD,&nbsp;Adivitch Sripusanapan MD,&nbsp;Kamonluk Rodsom MD,&nbsp;Urairat Chuenchaem MD,&nbsp;Ekamol Tantisattamo MD, MPH","doi":"10.1016/j.jnma.2024.07.058","DOIUrl":"10.1016/j.jnma.2024.07.058","url":null,"abstract":"<div><h3>Purpose</h3><p>Inflammation is widely recognized for its significant association with major adverse cardiovascular events (MACE). Our study aims to evaluate this relationship and predictive efficacy using the Systemic Inflammation Response Index (SIRI).</p></div><div><h3>Method</h3><p>In our study, we analyzed 9,450 adults aged 18 years and older from NHANES 2017-2018. We evaluated inflammatory status using the Systemic Inflammation Response Index (SIRI) and conducted ROC analysis to determine its predictive ability. Additionally, we employed three logistic regression models to assess the association of SIRI with Major Adverse Cardiovascular Events (MACE). The first model considered SIRI alone, the second model combined SIRI with hs-CRP and ferritin, and the third model included additional factors such as age, gender, race, education, asthma, diabetes, hypertension, and estimated glomerular filtration rate.</p></div><div><h3>Result</h3><p>ROC analysis was used to determine the SIRI cut-off points for predicting non-fatal myocardial infarction, stroke, angina, and heart failure, yielding values of 1.1195 (AUC=0.639, 95% CI: 0.606-0.672), 1.0594 (AUC=0.583, 95% CI: 0.549-0.616), 0.9882 (AUC=0.524, 95% CI: 0.506-0.543), and 1.1074 (AUC=0.646, 95% CI: 0.607-0.685), respectively. Despite various influencing factors in Model 3, SIRI showed significant associations with each MACE. These events included myocardial infarction (AOR=1.979, 95% CI: 1.537-2.548), stroke (AOR=1.399, 95% CI: 1.093-1.790), angina (AOR=1.979, 95% CI: 1.537-2.548), and heart failure (AOR=2.586, 95% CI: 1.742-3.837).</p></div><div><h3>Conclusion</h3><p>SIRI shows strong associations with all outcomes but only predicts non-fatal MI and heart failure. Despite this limitation, its cost-effectiveness and accessibility indicate potential as an early screening tool for improving risk assessment and intervention in high-risk individuals.</p></div>","PeriodicalId":17369,"journal":{"name":"Journal of the National Medical Association","volume":"116 4","pages":"Page 436"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicians’ perception on using a multi-cancer early detection blood test to reduce disparities in cancer screening","authors":"Garfield A. Clunie ,&nbsp;Sharon D. Allison-Ottey ,&nbsp;Joy D. Calloway ,&nbsp;Marie L. Borum","doi":"10.1016/j.jnma.2024.07.011","DOIUrl":"10.1016/j.jnma.2024.07.011","url":null,"abstract":"&lt;div&gt;&lt;h3&gt; Introduction&lt;/h3&gt;&lt;p&gt;&lt;span&gt;Cancer causes significant morbidity and mortality in the United States. It is the second most common cause of death in the United States, after heart disease. African Americans are disproportionately affected by malignancy, with overall higher death rates compared to other racial and ethnic groups. Screening tests can identify early stage malignancy allowing for timely intervention. However, African Americans less frequently undergo &lt;/span&gt;cancer screening. Advancement in genomic technology has led to the identification of signals for cancer in the blood. This has resulted in the development of multi-cancer early detection (MCED) tests which evaluate for circulating cell-free DNA (cfDNA). This study evaluated physicians’ perception of the use of a multi-cancer early detection test (MCED).&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;p&gt;An anonymous, 29 question survey was administered to African American / Black physicians and medical students. Survey participants were identified through the National Medical Association and other professional organizations that included primarily African American physicians. Surveys were excluded from analysis if respondent was non-African American / Black or was not a physician or medical student.&lt;/p&gt;&lt;p&gt;The survey collected physician demographics, percentage of African American / Black patients in their practice, patient barriers to screening, potential use of MCED tests and factors influencing decision to recommend testing. Descriptive statistics were generated. Additional analysis was performed using Chi-Square with statistical significance set at p-value &lt;0.05. The survey was pilot tested for reliability and validity.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;1196 (681 female, 515 male) physicians and medical students completed the survey. 95.8 % were physicians who were or had been in clinical practice. Fifty-three percent of physicians reported that &gt;40 % of their patients were African American / Black. Barriers to cancer screening included lack of understanding of the importance (33.8 %), lack of or limited insurance coverage (23.5 %), socioeconomic factors unrelated to insurance coverage (16.2 %), fear of cancer (8.8 %), history of racism and bias in the health care system (7.4 %) with 8.8 % reporting ‘other’ and 1.5 % reporting no perceived barriers. There was a significant difference (p&lt;0.03) in the rate that physicians’ perceived racism and bias in the health care system as barrier for cancer screening in African American / Black patients when compared to other patients.&lt;/p&gt;&lt;p&gt;Most physicians and medical students indicated that a MCED test would benefit all patients (86.8 %), would encourage further cancer screening tests (83.8 %), and would be beneficial for minority and under-represented patients regardless of socioeconomics or health care access (83.8 %). Seventy-five percent of survey respondents indicated that a MCED test would be beneficial in promoting further cancer screeni","PeriodicalId":17369,"journal":{"name":"Journal of the National Medical Association","volume":"116 4","pages":"Pages 325-327"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141716274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Who declines “opt-out” HIV/HCV testing? Experience of an internal medicine resident continuity clinic serving a predominantly Black adult population in South Carolina.","authors":"Jodian Pinkney ,&nbsp;Yao Tong ,&nbsp;Susanne Hoeppner ,&nbsp;Caroline Derrick ,&nbsp;Gregg Talente ,&nbsp;Rocio Hurtado ,&nbsp;Christina Psaros ,&nbsp;Bisola O. Ojikutu ,&nbsp;Laura M. Bogart ,&nbsp;Helmut Albrecht ,&nbsp;Divya Ahuja ,&nbsp;Emily Hyle","doi":"10.1016/j.jnma.2024.07.009","DOIUrl":"10.1016/j.jnma.2024.07.009","url":null,"abstract":"<div><h3>Background</h3><p>Universal “opt-out” human immunodeficiency virus (HIV) or hepatitis C virus (HCV) testing involves testing individuals for HIV or HCV regardless of symptoms, unless they decline. Little is known about the characteristics of individuals who decline.</p></div><div><h3>Methods</h3><p>We conducted a retrospective, medical record review of adults evaluated at an outpatient clinic in South Carolina. “Opt-out” HIV/HCV testing was implemented in Feb 2019; we reviewed medical records of individuals evaluated in May - July 2019. We excluded individuals who did not meet age-based screening criteria (HIV: 18–65 years; HCV: 18–74 years), had a prior HIV/HCV diagnosis, were tested for HIV/HCV within the preceding 12 months, and whose “opt-out” decision was not documented. We used multivariable logistic regression to estimate adjusted odds ratios (aOR) and 95 % confidence intervals (CI) for “opt-out” decision, with age, sex, race/ethnicity, insurance status, visit type, and genitourinary vs. non-genitourinary chief complaints as predictors.</p></div><div><h3>Results</h3><p>The final analyses included 706 individuals for HIV and 818 for HCV. Most individuals were non-Hispanic Black (77 % and 78 %) and female (66 % and 64 %). The mean ages were 49.1 (±11.9) and 51.9 (±13.2). Nearly one-third of individuals declined HIV and HCV testing (31 % and 30 %). Black males were more likely to decline HIV and HCV testing than Black females (aOR = 1.61 [95 % CI. 1.08 - 2.40] and aOR = 1.50 [95 %CI. 1.04 - 2.16]).</p></div><div><h3>Conclusion</h3><p>Despite HIV/HCV testing being the standard of care, approximately one-third of eligible individuals may decline testing, the demographic characteristics of whom may overlap with individuals who are traditionally unaware of their status.</p></div><div><h3>Main Point</h3><p>Despite HIV/HCV testing being the standard of care, approximately one-third of eligible individuals may decline testing, the demographic characteristics of whom may overlap with individuals who are traditionally unaware of their status.</p></div>","PeriodicalId":17369,"journal":{"name":"Journal of the National Medical Association","volume":"116 4","pages":"Pages 351-361"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0027968424000841/pdfft?md5=3b3bcd6e6cd9ab775c7f339be186660d&pid=1-s2.0-S0027968424000841-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141857562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breathing Life Into Learning About Air Quality","authors":"Katie Hoskins Degrees MLS, Med,&nbsp;Molla Fahad BS","doi":"10.1016/j.jnma.2024.07.025","DOIUrl":"10.1016/j.jnma.2024.07.025","url":null,"abstract":"<div><h3>Background</h3><p>The San Joaquin Valley (SJV) comprises diverse populations that are medically underserved and experience significant air pollution.</p></div><div><h3>Objective</h3><p>Provide education to health careers pathway high school students on hazards of air pollution to respiratory health, steps they can take to protect their health, and how to access the National Library of Medicine resources for reliable health information.</p></div><div><h3>Methods</h3><p>Two-hour interactive outreach sessions were provided at three local high schools in Fresno and Madera to students in health careers pathways. Librarians and student doctors from a local osteopathic medical school guided high school students in health professions pathways through activities to understand the causes of poor air quality in the SJV, describe the different parts of the respiratory system and how air pollutants impact it, identify steps they can take to monitor the air quality and adjust their activities to protect their health, and how zip code is a fairly accurate predictor of health, and to access National Library of Medicine resources for reliable health information.</p></div><div><h3>Results</h3><p>A comparison of pre- and post-surveys showed a significant improvement in understanding the air quality index (AQI), how air pollutants impact different body systems, and lifestyle modifications to reduce the health impacts of air pollution. Outreach sessions to high school students can effectively increase knowledge of environmental health issues.</p></div><div><h3>Conclusions</h3><p>This sample population showed a positive result between pre- and post-surveys following education on a focused health topic.</p></div>","PeriodicalId":17369,"journal":{"name":"Journal of the National Medical Association","volume":"116 4","pages":"Page 421"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142086998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Black Hairstyles in Radiological Imaging: A Pilot Study","authors":"Angela Udongwo MPH,&nbsp;Jariatu Kamara BA,&nbsp;Candrice R. Heath MD,&nbsp;Alex Kleinmann,&nbsp;Alex Sandberg BS,&nbsp;Gary Cohen MD,&nbsp;Hillel S. Maresky MD","doi":"10.1016/j.jnma.2024.07.099","DOIUrl":"10.1016/j.jnma.2024.07.099","url":null,"abstract":"<div><h3>Background</h3><p>Hairstyles common in Black communities, braids, twists, locs present as artifacts, posing unique diagnostic challenges in interpreting radiological imaging, though limited research has been conducted on the current climate of their interpretation.</p></div><div><h3>Purpose</h3><p>Present a review of hairstyles as hair artifacts in literature and investigate the comfortability and familiarity of physicians and radiology technicians in assessing braids, twists, and locs as hair artifacts on radiological imaging.</p></div><div><h3>Methods</h3><p>Cross-sectional study was conducted in an academic hospital using an online platform to assess the experiences and awareness of hairstyles on imaging. Responses were collected between April 2023 through August 2023 and included demographic information, career length, how often they came across hair artifacts in imaging, comfort and familiarity with Black hairstyles as artifact, and a quiz to distinguish pathology from hair artifact on chest x-ray images.</p></div><div><h3>Results</h3><p>46 participants from Temple Radiology (41.3%), Temple Emergency Medicine (21.7%), Temple Jeanes Campus (26.1%), and St. Lukes Bethlehem Radiology Group (2.2%), with the Radiologist cohort of an average experience of 9.40 years (SD=12.41), Emergency Medicine cohort 7.11 years (SD=7.08), and the Intern cohort had 0.5 years (SD=0.71). Career length (Row Factor: [9.8]; p &lt; 0.0001) and patient load (Row Factor: [3.1], p = 0.029; p &lt; 0.05) significantly affected self-reported scores, with longer careers and larger loads associated with higher score of comfort and familiarity among study participants.</p></div><div><h3>Conclusions</h3><p>Longer career lengths and a higher number of patients per day improved awareness of how Black hairstyles presents in medical imaging. This study highlighted a radiological phenomenon lacking in medical literature and provided valuable insights into the impact of experience on physicians' ability to identify and interpret radiological images affected by styles that can mimic pathology. An emphasis is put on the need for education earlier in training.</p></div>","PeriodicalId":17369,"journal":{"name":"Journal of the National Medical Association","volume":"116 4","pages":"Page 455"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142087947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Health Equity Implications of People Living With HIV","authors":"Jareese Stroud MPH,&nbsp;Maisha Standifer MPH, PhD,&nbsp;Evan Martin LMSW,&nbsp;Jammie Hopkins DrPH,&nbsp;Allyson Belton MPH,&nbsp;Donrie Purcell PhD,&nbsp;Eric Warren","doi":"10.1016/j.jnma.2024.07.048","DOIUrl":"10.1016/j.jnma.2024.07.048","url":null,"abstract":"<div><h3>Introduction</h3><p>HIV diagnosis rates are not evenly distributed and significantly impact black men and women residing in Georgia and Louisiana. Additionally, reporting in these specific states demonstrate late HIV diagnosis and a failure to link patients to care.</p></div><div><h3>Methods</h3><p>In response to this alarming issue, the “End the Epidemic” initiative l addresses structural barriers in HIV care impacting Black communities in Georgia, Louisiana by implementing: 1) Conduct asset mapping to examine the health systems gaps that prevent healthcare utilization for PLWHA. Train diverse healthcare professionals, learners and leaders, on the social/political determinants of health to develop sustainable and solutions-driven policies towards the achievement of health equity for PLWHA. 2) Assess jurisdictional policies which exacerbate/alleviate gaps in the HIV care continuum impacting PLWHA. Convene an HIV Equity Task Force to develop health equity strategies for retaining PLWHA. 3) Increase HIV data availability on the Health Equity Tracker to improve access to data for individuals and organizations. Provide technical assistance and policy assessments to jurisdictions concerning data practices, including tracking and measuring health inequities for PLWHA. Develop high-quality communications resources for community members, leaders, and influencers on HIV inequities. This initiative will apply a mixed methods approach to the data collection and analysis component of the project, as well as a process evaluation.</p></div><div><h3>Conclusion</h3><p>This novel approach will 1) Increase access and utilization of culturally competent healthcare to Black people impacted by the HIV epidemic 2) Gain insight into the disruption of the healthcare delivery system due to COVID-19 in the Black community and realign HIV service for PLWHA 3) Influence clinical provider training in HIV and 4) Initiate early engagement with experienced clinicians practicing in Black communities in the aforementioned cities.</p></div>","PeriodicalId":17369,"journal":{"name":"Journal of the National Medical Association","volume":"116 4","pages":"Pages 431-432"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding Cancer: PVT1 Intron 10’s homology with MCM3","authors":"Eleonora Achrak BS","doi":"10.1016/j.jnma.2024.07.017","DOIUrl":"10.1016/j.jnma.2024.07.017","url":null,"abstract":"<div><h3>Purpose</h3><p>Prostate cancer remains a leading cause of mortality among men, partly due to challenges in early detection. This study investigates Plasmacytoma Variant Translocation 1 (PVT1) intron 10, hypothesized to overexpress in prostate cancer tissues and exhibit structural homology with the DNA replication protein MCM3, potentially defying the notion of introns as mere non-coding sequences.</p></div><div><h3>Methods</h3><p>An analysis of 550 patient-derived prostate adenocarcinoma transcripts was conducted. Transcript overexpression was quantified using TSVdb, and genetic/structural homology was determined through BLAST against major databases. HotSpot Wizard assessed 3D structural homology, while SpliceRover detected splicing motifs. Clinical relevance was verified by comparing expression levels in the dbGaP database.</p></div><div><h3>Results</h3><p>Significant overexpression of PVT1 intron 10 was found, with a 4.3-fold increase noted in specific exons and introns. Proteomic homology analysis revealed an 85% similarity with essential proteins, including a 68.8% structural alignment with MCM3. The 3D structural comparisons supported these findings. Database validation confirmed a 5.3-fold overexpression of intron 10 in cancerous tissues.</p></div><div><h3>Conclusion</h3><p>PVT1 intron 10′s significant overexpression and structural similarity to MCM3 protein in prostate cancer tissue suggests a potential functional role, disrupting the conventional understanding of intronic regions. These insights position intron 10 as a promising biomarker and therapeutic target, offering new directions in prostate cancer diagnostics and treatment through a refined understanding of alternative splicing and protein homology.</p></div>","PeriodicalId":17369,"journal":{"name":"Journal of the National Medical Association","volume":"116 4","pages":"Page 417"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Follicular Lymphoma Stem Cell Biomarkers","authors":"Daniel P Ashley MPA, BSc,&nbsp;Theodor Borgovan MD,&nbsp;Hyung Sun Yoo MBA,&nbsp;Xin Zhang MD, PhD,&nbsp;Samantha Ahrens,&nbsp;Qingyang Luo,&nbsp;Li Huan MD,&nbsp;John Cole MD,&nbsp;Li Li MD, PhD","doi":"10.1016/j.jnma.2024.07.051","DOIUrl":"10.1016/j.jnma.2024.07.051","url":null,"abstract":"<div><h3>Introduction</h3><p>Despite recent advances in identifying clinical risk factors for follicular lymphoma (FL), there remains a need for prognostic and predictive biomarkers. In this study, our objective was to identify biomarkers that are reliable indicators of FL relapse and overall survival via high-throughput screening using tissue microarray (TMA).</p></div><div><h3>Methods</h3><p>Records of patients diagnosed with FL between 1982 and 2009 were examined, with results sorted based on survival post-diagnosis. Corresponding patient biopsies were collected to create TMAs for high-throughput immunohistochemistry (IHC) screening of putative FL cancer stem cell (F-SC) markers (ABCG2, Ki67, OCT3/4) and CD20. Positivity was analyzed via digital batch processing method using Image-Pro software and microscopy.</p></div><div><h3>Results</h3><p>Fifty-nine patients were partitioned into short -(&lt;5 years, n=26) and long- (&gt;15 years, n=33) survival groups. IHC results showed there was no statistically significant difference in CD20 expression (p=0.6378). The results showed an increased expression pattern with significance for all 3 FL-SC markers. Compared with the long-survival group, the short-survival group had significantly higher expression levels of Ki67 (p=0.0275), ABCG2 (p=0.0251) and OCT3/4 (p=0.0226), as well as the combination of all three biomarkers (p=0.0229).</p></div><div><h3>Conclusion</h3><p>The prognostic biomarkers for FL identified may be used to distinguish those patients who are at greatest risk of relapse and in need of the most aggressive and novel therapies. Qualified prognostic markers for FL may direct clinical decision paradigms on which patients are favorable candidates for early therapy, and will obtain additional insight in the development of targeted regiments and treatment protocols to ameliorate outcomes.</p></div>","PeriodicalId":17369,"journal":{"name":"Journal of the National Medical Association","volume":"116 4","pages":"Page 433"},"PeriodicalIF":2.5,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}