Journal of The Japanese Society for Horticultural Science最新文献

{"title":"Morphology of Sterile Anthers and Inheritance of Cytoplasmic-genetic Male Sterility in Zygotic Seedlings of Polyembryonic Acid Citrus","authors":"Prita Sari Dewi, A. Wakana, Y. Tanimoto, Y. Fujiwara, K. Sakai, Kohei Kajiwara","doi":"10.2503/JJSHS1.82.203","DOIUrl":"https://doi.org/10.2503/JJSHS1.82.203","url":null,"abstract":"In acid citrus, genetic control of male sterility, an important characteristic for breeding seedless fruit cultivars, is not precisely known because of the presence of barriers such as polyembryony and a long juvenile phase. In this study, 22 crosses with 16 male-fertile acid citrus cultivars were carried out and the zygotic seedlings were grafted onto adult satsuma mandarin trees to enhance flowering. Four crosses with two monoembryonic and male-sterile citrus plants (HY16 and ‘Kiyomi’) were also carried out and zygotic seedlings showing precocious flowering were used to examine the inheritance of male sterility. Of the 26 crosses, 21 with six cultivars as seed parents generated male-sterile and male-fertile zygotic seedlings with various segregation ratios, whereas five crosses with ‘Sudachi’ as a seed parent generated only male-fertile zygotic seedlings. The sterile anthers were categorized into undeveloped and underdeveloped by their size in most progenies. The result of these crosses showed that eight cultivars with male-sterile cytoplasmic factors of ‘Yuzu’, lemon, or satsuma mandarin generated male-sterile zygotic seedlings, while ‘Sudachi’ with male-fertile cytoplasmic factors of pummelo generated only male fertile zygotic seedlings. The result also suggested that a dominant nuclear fertility-restorer gene system comprising one epistatic gene R1 and two complementary genes R2 and R3 controls the restoration of male fertility and male-sterile anther size in acid citrus plants with sterile cytoplasm (S). The complementary gene R3 is located downstream from R2, and gene R1 is epistatic to R2 and R3 genes. Genotypes (S)r1r1r2r2-are responsible for undeveloped anthers while genotypes (S)r1r1R2-r3r3 are responsible for underdeveloped anthers. The identified genotypes for male sterility in major acid citrus cultivars will contribute to breeding seedless acid cultivars.","PeriodicalId":17343,"journal":{"name":"Journal of The Japanese Society for Horticultural Science","volume":"82 1","pages":"203-214"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69157546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Torenia (Torenia fournieri Lind. ex Fourn.) Novel Mutant 'Flecked' Produces Variegated Flowers by Insertion of a DNA Transposon into an R2R3-MYB Gene","authors":"T. Nishijima, Yasumasa Morita, K. Sasaki, M. Nakayama, H. Yamaguchi, N. Ohtsubo, T. Niki, T. Niki","doi":"10.2503/JJSHS1.82.39","DOIUrl":"https://doi.org/10.2503/JJSHS1.82.39","url":null,"abstract":"A novel torenia (Torenia fournieri Lind. ex Fourn.) mutant ‘flecked’, which bears variegated flowers, was obtained from ethyl methanesulfonate-treated M2 plants. The lower lip of this mutant has small violet spots with a pale violet background, while that of the normal type is solid violet. The mutant trait frequently reverted to a semicircular violet sector or solid violet lower lip. Germinal revertant plants with a solid violet lower lip also frequently occurred in S1 plants derived from self-pollinated mutant flowers. In the lower lip of the mutant type, anthocyanin concentration was much lower than in the normal type. This was attributed to decreased expression of the genes encoding anthocyanin biosynthesis enzymes, i.e. torenia chalcone synthase (TfCHS), flavanone 3hydroxylase (TfF3H), dihydroflavonol 4-reductase (TfDFR), anthocyanidin synthase (TfANS), and UDP-glucose 3-O-flavonoid glucosyltransferase (TfUFGT). In the lower lip of the mutant, expression of a gene encoding R2R3MYB transcription factor (TfMYB1, Torenia fournieri MYB1) was much lower than in the normal type and the revertants; this was caused by insertion of a Enhancer/Suppressor-Mutator (En/Spm)-like transposon (Ttf1, Transposon Torenia fournieri 1) in the 2nd intron of TfMYB1. Furthermore, it was found that the reversion of anthocyanin accumulation in the lower lips correlated to excision of Ttf1 from the TfMYB1. Overexpression of TfMYB1 in torenia caused anthocyanins to accumulate in the purple callus as a result of enhanced expression of the five structural genes mentioned above, demonstrating that TfMYB1 regulates these genes. Therefore, we concluded that a homozygous allele of Ttf1-inserted TfMYB1 caused the mutant phenotype. Ttf1 is a nonautonomous element because Ttf1 does not have the DNA sequence encoding transposase. Based on these results, potential uses of the flecked mutant for torenia breeding and transposon tagging are discussed.","PeriodicalId":17343,"journal":{"name":"Journal of The Japanese Society for Horticultural Science","volume":"82 1","pages":"39-50"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2503/JJSHS1.82.39","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69157644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variation of Dormancy and Early Flowering Ability in Lilium longiflorum and L. formosanum Populations in the Ryukyu Archipelago and Taiwan","authors":"N. Mojtahedi, J. Masuda, M. Hiramatsu, N. T. Hai, Y. Mizunoe, H. Okubo","doi":"10.2503/JJSHS1.82.234","DOIUrl":"https://doi.org/10.2503/JJSHS1.82.234","url":null,"abstract":"Variations in the dormancy and early flowering ability of seedlings of four populations of Lilium longiflorum, Yaku Shima (LYA), Kikai Jima (LKI), and Ishigaki Jima (LIS) in the Ryukyu Archipelago, Japan and Pitouchiao (LPI), Taiwan, and two populations of L. formosanum, Wulai (FWU), Taiwan and a domesticated Fukuoka population (FFU), Japan were investigated. Seedlings of each population were grown at 15°C for five months. They were then transplanted into an experimental open field for two years or at 15, 20, 25, and 30°C under natural day length for 22 weeks. In the field experiments, FWU, FFU, and LPI populations continued to develop new leaves even at >30°C. Flowering percentage for FFU and LPI was 90% and 19.7%, respectively. Leaf development of LIS, LKI, and LYA was completely arrested from early June in both years, and the flowering percentages were 28, 25, and, 10 in the second year, respectively. Under controlled temperature conditions, LKI and LYA populations produced new leaves only at 15°C. FFU and LPI continued growing at 25 and 30°C, whereas the other populations did not grow. FFU significantly produced the heaviest leaves and bulbs and the highest number of scales per bulb at any temperature. The results showed that high temperature induces bulb dormancy in northern L. longiflorum. Strong correlation with the early flowering ability and bulb dormancy was also found. It seems that the early flowering ability of L. formosanum is largely dependent on the lack or reduction of bulb dormancy after adaptation to the local southern climate. The latitudinal variation of this trait demonstrated the geographic gradient during species habituation in the Ryukyu Archipelago.","PeriodicalId":17343,"journal":{"name":"Journal of The Japanese Society for Horticultural Science","volume":"82 1","pages":"234-241"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69157727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-compatibility in Interspecific Hybridization of Lychnis (Caryophyllaceae) and Characterization of Interspecific Hybrids between L. fulgens and L. sieboldii","authors":"M. Nakano, S. Kuwayama, E. Oka, Megumi Asano, Dong-sheng Han, T. Godo","doi":"10.2503/JJSHS1.82.57","DOIUrl":"https://doi.org/10.2503/JJSHS1.82.57","url":null,"abstract":"Lychnis belongs to Caryophyllaceae and contains a number of horticulturally attractive species. In order to widen their variations in horticultural traits, interspecific cross-pollination and subsequent immature seed culture were carried out using 8 Lychnis species, L. chalcedonica, L. coronata, L. fulgens, L. gracillima, L. kiusiana, L. miqueliana, L. sieboldii, and L. wilfordii. Enlarged fruits containing immature seeds were obtained in the 26 cross-combinations 4 weeks after pollination. Immature seeds were isolated from the fruits and cultured on halfstrength Murashige and Skoog medium without plant growth regulators, on which germination occurred in 11 cross-combinations and seedlings were produced in 8 cross-combinations. However, green seedlings were obtained only in L. fulgens × L. sieboldii, and seedlings obtained from the other 7 cross-combinations, L. coronata × L. gracillima, L. gracillima × L. coronata, L. kiusiana × L. fulgens, L. kiusiana × L. sieboldii, L. kiusiana × L. wilfordii, L. wilfordii × L. kiusiana, and L. wilfordii × L. sieboldii, were albino and died during acclimatization. The hybridity of all 55 green seedlings obtained from L. fulgens × L. sieboldii was confirmed by random amplified polymorphic DNA analysis. Although some morphological variations were observed among hybrids, hybrid plants generally showed intermediate morphologies between the parents. Hybrids showed high pollen fertility and their selfpollination yielded viable seeds. The present study shows the possibility of interspecific cross-breeding in Lychnis. Further studies are necessary to improve the culture conditions of immature seeds and to examine interspecific cross-compatibility using a wide range of Lychnis species.","PeriodicalId":17343,"journal":{"name":"Journal of The Japanese Society for Horticultural Science","volume":"82 1","pages":"57-62"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69157741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Sample Preparation Method for Direct and Non-direct PCR in Woody Plants","authors":"S. Ohta, K. Yano, Yuki Kurita, M. Kita, T. Shimizu, H. Nesumi","doi":"10.2503/JJSHS1.82.14","DOIUrl":"https://doi.org/10.2503/JJSHS1.82.14","url":null,"abstract":"Despite recent advances in DNA analysis techniques, the process of acquiring purified DNA is particularly costly and time-consuming in woody plants. The ability to obtain purified DNA is frequently hindered by the presence of secondary compounds, including polyphenols and polysaccharides. In this study, we developed a sample preparation method for direct and non-direct PCR that is highly reproducible and cost-effective. This method entails pricking a leaf sample with a toothpick and dissolving the collected DNA in a PCR mixture or TE0.2 buffer solution. We confirmed the utility of the method using seedlings derived from backcrossed hybrids of Citrus hassaku hort. ex Y. Tanaka × Poncirus trifoliata (Linn.) Raf. Using a direct PCR method, we obtained 99% amplification success rate for the 1200 bp CTg12A region. PCR amplification from DNA extracts, which was also produced by our method, succeeded in 96–100% of samples for five different DNA markers, except for CTg12A, where it was only 86%. We were able to process and commence PCR amplification of 95 leaf samples in 1.5–2.0 h, in contrast to 23 h using conventional methods. We also demonstrated the broader applicability of this method by extending our study to 18 other fruit trees and ornamental woody plants. PCR products were generated for all 18 tested species using chloroplast accD and rbcL markers, and for Japanese chestnut, mango, and Japanese pear using their species-specific SSR markers. Our new method might be suitable for analyses which handle large number of samples, such as marker-assisted selection and cultivar discrimination, and holds promise for use in DNA marker typing in various kinds of woody plants.","PeriodicalId":17343,"journal":{"name":"Journal of The Japanese Society for Horticultural Science","volume":"82 1","pages":"14-21"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2503/JJSHS1.82.14","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69157447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Temperature in Dormancy Induction and Release in One-year-old Seedlings of Lilium longiflorum Populations","authors":"N. Mojtahedi, J. Masuda, M. Hiramatsu, N. T. Hai, H. Okubo","doi":"10.2503/JJSHS1.82.63","DOIUrl":"https://doi.org/10.2503/JJSHS1.82.63","url":null,"abstract":"The role of temperature in bulb dormancy induction and release was investigated in one-year-old seedlings of three populations of Lilium longiflorum, i.e., Yakushima (LYA) and Kikaijima (LKI) in the Ryukyu Archipelago, Japan and Pitouchiao (LPI), Taiwan, and two populations of L. formosanum, Wulai (FWU), Taiwan and domesticated Fukuoka population (FFU), Japan. LYA and LKI undergo deep summer dormancy, while LPI, FWU and FFU do not. Four weeks of high temperature induced dormancy in LYA and LKI, whereas two weeks of high temperature was insufficient. Dormancy was not induced in LPI and FFU even after eight weeks of high temperature. Re-growth after dormancy was initiated in LKI and LYA six to eight weeks after shifting to 15°C. In the temperature-controlled experiment, all the populations continued developing new leaves at 15°C for 22 weeks, and stopped leaf production at 20 and 25°C in winter, while FFU produced new leaves at 30°C. It is considered that summer dormancy induction and release in one-year-old seedlings of northern ecotypes of L. longiflorum is determined by the duration of high and low temperature, respectively. It was proved that development of nonor weakly dormant ecotypes of L. longiflorum and L. formosanum is associated with the lack or reduction of sensitiveness to high temperature.","PeriodicalId":17343,"journal":{"name":"Journal of The Japanese Society for Horticultural Science","volume":"82 1","pages":"63-68"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2503/JJSHS1.82.63","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69158252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality Maintenance of Postharvest Horticultural Crops by Stress Treatments and Approach for the Elucidation of Its Mechanism","authors":"N. Yamauchi","doi":"10.2503/JJSHS1.82.1","DOIUrl":"https://doi.org/10.2503/JJSHS1.82.1","url":null,"abstract":"This review deals with the effectiveness and mechanism of stress treatments, such as heat and UV treatments (hot air, hot water, and UV-B), on the quality maintenance of postharvest green horticultural crops such as broccoli florets and green sour citrus fruits (lime, Nagato-yuzukichi, and yuzu), in which chlorophyll (Chl) degradation is a main factor in quality deterioration. Postharvest stress treatments effectively suppressed Chl degradation with senescence during storage. In broccoli florets and lime fruit, hydrogen peroxide, which is produced by postharvest stress treatments, could induce the activation of the ascorbate-glutathione (AsA-GSH) cycle, and enhancement of the AsA-GSH cycle might be involved in the suppression of senescence in such produce. The mechanism of peroxidase-mediated Chl degradation and the control of Chl-degrading enzymes by stress treatments were determined using broccoli florets and Nagato-yuzukichi fruit. Peroxidase oxidized phenolic compounds such as apigenin, naringin and p-coumaric acid, which have a hydroxyl group at the p-position, to form phenoxy radicals, and Chl a could be degraded by the formed phenoxy radicals to colorless low-molecularweight compounds. It was found that the cationic isoperoxidase related to Chl degradation, which is localized in the chloroplast, increased with senescence during storage and enhancement of the activity was effectually inhibited by stress treatments. In addition to peroxidase, the activities and gene expression of other Chl-degrading enzymes were controlled by stress treatments. Thus, stress treatments seem to be a useful method for controlling Chl degradation in stored green fruits and vegetables.","PeriodicalId":17343,"journal":{"name":"Journal of The Japanese Society for Horticultural Science","volume":"82 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2503/JJSHS1.82.1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69157053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome Analysis of Giant Pear Fruit with Fruit-specific DNA Reduplication on a Mutant Branch","authors":"K. Nashima, H. Takahashi, M. Nakazono, T. Shimizu, C. Nishitani, Toshiya Yamamoto, A. Itai, K. Isuzugawa, T. Hanada, T. Takashina, Mari Kato, S. Matsumoto, A. Oikawa, K. Shiratake","doi":"10.2503/JJSHS1.82.301","DOIUrl":"https://doi.org/10.2503/JJSHS1.82.301","url":null,"abstract":"Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan Okitsu Citrus Research Station, NARO Institute of Fruit Tree Science, Shizuoka 424-0292, Japan NARO Institute of Fruit Tree Science, Tsukuba 305-8605, Japan Faculty of Agriculture, Tottori University, Tottori 680-8550, Japan Horticultural Experiment Station, Yamagata Integrated Agricultural Research Center, Sagae 991-0043, Japan Riken Plant Science Center, Tsuruoka 997-0052, Japan Faculty of Agriculture, Yamagata University, Tsuruoka 997-8555, Japan","PeriodicalId":17343,"journal":{"name":"Journal of The Japanese Society for Horticultural Science","volume":"82 1","pages":"301-311"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2503/JJSHS1.82.301","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69157413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of the Timing of Fertilizer Application on the Number of Flowers of Japanese Pepper","authors":"T. Maeda, Y. Yonemoto, H. Higuchi, M. Hossain, Masayuki Taniguchi, Kazunari Hattori","doi":"10.2503/JJSHS1.82.11","DOIUrl":"https://doi.org/10.2503/JJSHS1.82.11","url":null,"abstract":"This study was conducted in three consecutive years to determine the appropriate timing of fertilizer application to increase the flower number of the Japanese pepper, Zanthoxylum piperitum (L.) DC. f. inerme Makino (known as ‘Budousanshou’ in Japan) cultivated in Wakayama Prefecture. The timing experiment involved three treatment options: fertilizer application in spring with two equal applications in May and June, a summer/autumn treatment of two equal applications in August and September, or a spring/summer/autumn treatment of four equal applications in May, June, August, and September. Each treatment consisted of an annual application of 300 kg ⋅ ha − 1 nitrogen. The spring treatment resulted in increased flower number. It was suggested that the application of fertilizer in the May/June spring treatment resulted in a focused accumulation of nutrients prior to the floral evocation period and led to the subsequent increase in flower number and cluster number relative to the summer/autumn and spring/summer/autumn treatments.","PeriodicalId":17343,"journal":{"name":"Journal of The Japanese Society for Horticultural Science","volume":"17 1","pages":"11-13"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69157113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in Cell Number, Osmotic Potential and Concentrations of Carbohydrates and Inorganic Ions in Tweedia caerulea during Flower Opening","authors":"R. Norikoshi, H. Imanishi, K. Ichimura","doi":"10.2503/JJSHS1.82.51","DOIUrl":"https://doi.org/10.2503/JJSHS1.82.51","url":null,"abstract":"The number of epidermal cells, osmotic potential, and carbohydrate and inorganic ion concentrations in petals during development and opening of Tweedia caerulea D. Don flowers was studied. The number of adaxial epidermal cells was greater than that of abaxial epidermal cells at all stages. The increase in cell number stopped at the stage just before flower opening. The size of adaxial and abaxial epidermal cells increased during flower development and opening. The results indicate that petal growth before flower opening depended on cell division and expansion, and petal growth during flower opening was attributable to petal cell expansion. Osmotic potential decreased and fructose, glucose and sucrose concentrations in the petals gradually increased during flower opening. Starch content and total inorganic ion concentration were almost constant during flower opening. Decreased osmotic potential is mainly attributed to increased glucose, fructose and sucrose concentrations. It is concluded that an increase in these sugar concentrations largely contributes to the decrease in osmotic potential. This decrease may facilitate water influx to cells, thereby maintaining pressure potential, which is apparently involved in petal cell expansion associated with flower opening.","PeriodicalId":17343,"journal":{"name":"Journal of The Japanese Society for Horticultural Science","volume":"82 1","pages":"51-56"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.2503/JJSHS1.82.51","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69157691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}