Journal of the Hellenic Veterinary Medical Society最新文献

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First report of vancomycin resistant Enterococcus faecalis and Enterococcus faecium isolated from water buffalo clotted cream in Turkey 土耳其首次报道从水牛凝乳中分离出耐万古霉素的粪肠球菌和粪肠球菌
4区 农林科学
Journal of the Hellenic Veterinary Medical Society Pub Date : 2023-10-18 DOI: 10.12681/jhvms.30877
E Seker, YN Dogan
{"title":"First report of vancomycin resistant Enterococcus faecalis and Enterococcus faecium isolated from water buffalo clotted cream in Turkey","authors":"E Seker, YN Dogan","doi":"10.12681/jhvms.30877","DOIUrl":"https://doi.org/10.12681/jhvms.30877","url":null,"abstract":"This study aimed to isolate the Enterococcus species from homemade water buffalo clotted cream sold in Afyonkarahisar, investigate the vancomycin resistance genes in isolated strains by PCR and determine the antibiotic resistance of strains to some antibiotics commonly used in Turkey. A total of 107 buffalo clotted cream samples sold in public bazaars of Afyonkarahisar were collected. Following the phenotypic identification using a commercial identification kit, PCR was applied to strains using by species specific primers. Forty Enterococcus strains were obtained from 107 samples by PCR. Of 40 strains, 31 (77.5%) and 9 (22.5%) were identified to be E. faecalis and E. faecium, respectively. One vanA (2.5%) and 14 vanB (35%) genes were determined in tested 40 strains. While of strains having the vanB gen, 10 and four were found to be E. faecalis and E. faecium, respectively, one E. faecium strain also harboured the vanA gen. Thus, while the vanB gen positivity was found to be 32.3% in 31 E. faecalis strains and 44.4% in 9 E. faecium strains, the vanA gen positivity was determined to be 11.1% in 9 E. faecium strains. It was not found the vanC1 and vanC2/C3 genes in none of the strains. While all of strains were phenotypically resistant to streptomycin and fusidic acid, high resistance rates were also determined in the strains to kanamycin (87.5%), cephalothin (80%), erythromycin (80%), gentamicin (77.5%), tetracycline (75%) and vancomycin (60%). Resistance to all tested antibiotics except teicoplanin was determined in E. faecalis strains and except chloramphenicol in E. faecium strains harbouring the vancomycin resistance genes. In conclusion, it was thought the buffalo clotted creams offered for consumption in Afyonkarahisar may have potential risk for public health in terms of VRE species. To our knowledge, this is the first study showing the presence of VRE in the clotted cream samples by PCR in Turkey.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of caseous lymphadenitis agent (corynebacterium pseudotuberculosis) isolated from superficial abscesses of sheep on oxidative stress factors 绵羊浅表脓肿分离的干酪样淋巴结炎剂(假结核棒状杆菌)对氧化应激因子的影响
4区 农林科学
Journal of the Hellenic Veterinary Medical Society Pub Date : 2023-10-18 DOI: 10.12681/jhvms.31218
E Polat, E Kaya, B Karagülle, H Akin
{"title":"Effects of caseous lymphadenitis agent (corynebacterium pseudotuberculosis) isolated from superficial abscesses of sheep on oxidative stress factors","authors":"E Polat, E Kaya, B Karagülle, H Akin","doi":"10.12681/jhvms.31218","DOIUrl":"https://doi.org/10.12681/jhvms.31218","url":null,"abstract":"In this study, it was aimed to determine the incidence of Corynebacterium pseudotuberculosis (C. pseudotuberculosis) in caseous lymphadenitis cases in superficial lymph nodes of sheep and to evaluate its effect on oxidative stress factors. Thus, it was aimed to determine the early diagnosability of superficial and visceral caseous lymphadenitis cases and to prevent the spread of diseases and related economic losses. A total of 103 sheep, 50 of which were healthy and 53 of which had caseous lymphadenitis, were evaluated in the study. Microbiological examinations were performed by taking 3-5 ml of pyogenic aspirate from the superficial lymph nodes of sheep with caseous lymphadenitis. Blood samples were taken from sheep with C. pseudotuberculosis isolated in microbiological examinations and the levels of oxidative stress factors were determined. C. pseudotuberculosis was isolated in 23 of the pyogenic aspirates of 53 sheep with caseous lymphadenitis. When sheep isolated from C. pseudotuberculosis were compared with healthy sheep, it was determined that there was a statistically significant decrease in the levels of antioxidant molecules such as GSH-pX (14.62%), GSH (23.81%), SOD (4.70%), CAT (22.23%) (p˂0.001). The level of toxic malondialdehyde (MDA) (18.62%), the end product of lipid peroxidation, was found to be statistically significantly increased in sheep isolated from C. pseudotuberculosis (p˂0.001). As a result, it was determined that oxidative stress factors showed statistically significant variability in cases of superficial caseous lymphadenitis (caused by C.pseudotuberculosis). For this reason, by determining the levels of oxidative stress factors in suspected herds, it was possible to make early diagnosis of the superficial and visceral forms of caseous lymphadenitis, and a basis was established for future studies.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Survey on the gastrointestinal parasites in Passeriformes and Psittaciformes with a focus on zoonotic parasites 过路虫和鹦鹉形虫胃肠道寄生虫的调查,以人畜共患寄生虫为重点
4区 农林科学
Journal of the Hellenic Veterinary Medical Society Pub Date : 2023-10-18 DOI: 10.12681/jhvms.31398
F Talazadeh, MH Razijalali, N Roshanzadeh, P Davoodi
{"title":"Survey on the gastrointestinal parasites in Passeriformes and Psittaciformes with a focus on zoonotic parasites","authors":"F Talazadeh, MH Razijalali, N Roshanzadeh, P Davoodi","doi":"10.12681/jhvms.31398","DOIUrl":"https://doi.org/10.12681/jhvms.31398","url":null,"abstract":"Considering the increase in the keeping of pet birds and the importance of gastrointestinal parasitic infection, especially zoonotic parasites, this study was conducted to determine the prevalence of gastrointestinal parasites in different species of Passeriformes and Psittaciformes. In this study, one hundred and twenty fresh fecal samples were collected from different species of captive pet birds in Ahvaz from Apr 2021 to Aug 2021. The collected samples were analyzed using Clayton-Lane methods, Modified Ziehl–Neelsen, and Trichrome staining. Light microscopic morphometry was used for the identification of helminth eggs and oocysts. The results of this study showed that 37 samples (30.83%) were infected with gastrointestinal parasites. Among the helminthic parasites, three samples (2.5%) were infected with Hymenolepis spp.. Among protozoan parasites, 24 samples (20%) were infected with Eimeria spp., three samples (2.5%) were infected with Isospora spp., two samples (1.66%) were infected with Cryptosporidium spp., and five samples (4.16%) were infected with Giardia spp.. The results of the present study showed that the highest rate of gastrointestinal parasitic infection was in Passeriformes kept in pet shops and the prevalence of gastrointestinal parasitic infection was higher in the zebra finch, common mynah, and canary compared to other pet birds. But the highest rate of zoonotic parasitic infection was in Psittaciformes. According to the results, zoonotic parasites are present in pet birds in the Ahvaz area and should be considered by the owners of these pet birds, breeders, veterinarians, and public health organizations.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nutritive value and in-vitro digestibility of peels and pomaces of different citrus species 不同柑橘品种果皮和果渣的营养价值及体外消化率
4区 农林科学
Journal of the Hellenic Veterinary Medical Society Pub Date : 2023-10-18 DOI: 10.12681/jhvms.31044
OO Olowu, S Yaman Firincioglu
{"title":"Nutritive value and in-vitro digestibility of peels and pomaces of different citrus species","authors":"OO Olowu, S Yaman Firincioglu","doi":"10.12681/jhvms.31044","DOIUrl":"https://doi.org/10.12681/jhvms.31044","url":null,"abstract":"The nutritive value and invitro digestibility of agro-industrial byproducts of Citrus aurantium, Citrus paradisi, Citrus reticulata, Citrus limon and Citrus sinensis were assessed for their feed modification potential. Nutritive value assessment was done through chemical analysis while the ANKOM Daisy II incubator method was used to determine the invitro digestibility. At p>0.05, there was no statistical significance for nutritive value of the citrus byproducts for the five species. The agro-industrial by-products had significantly different phenolic content. Invitro digestibility of the peels significantly differed from the pomaces (p<0.001). The byproducts of the five citrus species showed potential to be considered as non-forage energy sources in ruminant feed. Lemon byproducts had high protein but not sufficient as a single protein source while bitter orange pomace may be considered for antioxidant capacity and high digestibility. Abundance of citrus agro-industrial byproducts, nutritive value potentials and their digestibility makes it a good strategy for ruminant feed modification and utilization.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135942824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The influence of various rates of royal jelly enriched extenders on frozen/thawed sperm quality and fertility in goats 不同蜂王浆添加量对山羊冻/融精子质量和生育能力的影响
4区 农林科学
Journal of the Hellenic Veterinary Medical Society Pub Date : 2023-10-18 DOI: 10.12681/jhvms.30798
N Coskun Cetin, F Karaca
{"title":"The influence of various rates of royal jelly enriched extenders on frozen/thawed sperm quality and fertility in goats","authors":"N Coskun Cetin, F Karaca","doi":"10.12681/jhvms.30798","DOIUrl":"https://doi.org/10.12681/jhvms.30798","url":null,"abstract":"The goal of this study was to detect the influence of royal jelly which has a strong antioxidant capacity supplementation in TRIS egg yolk and TRIS soybean lecithin extenders on quality and fertility. Sperm was collected from 5 Damascus bucks via an electro ejaculator during the breeding season. After dilution and cooling, the lowest motility and membrane integrity were detected in TEY-1RJ, in terms of abnormal spermatozoon rate, the TEY-1RJ group was the highest in cooled semen (P<0.05). After dilution and cooling, osmolality decreased significantly when the RJ ratio increased (P<0.05). After freezing-thawing, in both TEY and TSL extenders, the lowest sperm quality was detected in the 1% RJ groups. In terms of intact acrosome ratio, the highest value was recorded in the 0.50% RJ group in TEY and TSL diluents (P<0.05). Fertility assessments were conducted with 150 goats synchronized with intravaginal sponges and goats were inseminated transcervical. There was no difference between pregnancy rates between TEY and TSL-control and RJ groups (P>0.05). As a result, it was deduced that RJ additions to TEY and TSL extenders did not produce any alternative to freezing on sperm quality in Damascus goat semen except for the intact acrosome ratio and had no positive effect on fertility.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of In-Ovo Glucose and Glutamine Treatment on Hatching Efficiency, Intestinal Histomorphology and Gene Expression of Digestive Enzymes in Broiler Chicks 蛋内葡萄糖和谷氨酰胺处理对肉仔鸡孵化效率、肠道组织形态和消化酶基因表达的影响
4区 农林科学
Journal of the Hellenic Veterinary Medical Society Pub Date : 2023-10-18 DOI: 10.12681/jhvms.30895
F Cönerr, P Saçaklı
{"title":"Effects of In-Ovo Glucose and Glutamine Treatment on Hatching Efficiency, Intestinal Histomorphology and Gene Expression of Digestive Enzymes in Broiler Chicks","authors":"F Cönerr, P Saçaklı","doi":"10.12681/jhvms.30895","DOIUrl":"https://doi.org/10.12681/jhvms.30895","url":null,"abstract":"The purpose of the present study was to identify the effects of in-ovo injection of glucose and glutamine on hatching efficiency, hatching weight, liver and residual yolk weights, intestinal histomorphology and gene expressions of digestive enzymes. On the 17th day of incubation, NaCl (0.9 %), glutamine (0.5 ml 10%), glucose (0.5 ml 0.25 g/ml), and glutamine+glucose (0.25 ml 10%+0.25 ml 0.25 g/ml) were injected into the amniotic sac. In-ovo injection of glucose, glutamine and glucose+glutamine significantly decreased hatching efficiency in glucose and glutamin+glucose groups (p<0.001). This treatment did not affect hatching weight, liver and residual yolk weight in any of the groups. When the intestinal histomorphology was evaluated, in-ovo injection was found to increase the villi height/crypt depth ratio, villus width and the number of goblet cells in the jejunum; whereas villi height, crypt depth, and tunica muscularis thickness were not significantly affected by the treatments. Moreover, the ileum histomorphology in general appear not to be affected by any of the treatments as well. In-ovo glutamine injection significantly increased Suc-Iso and mTOR gene expressions (p≤0.05) compared to the control group, whereas SGLT1 gene expression was statistically indifferent. At the end of the study, while in-ovo glucose and glutamine injection had an overall negative effect on the hatching efficiency in broiler chicks.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Solid State Fermentation with Whey on Nutrient Composition of Pomegranate Peel Supplemented Sunflower Meal 乳清固体发酵对石榴皮添加向日葵粕营养成分的影响
4区 农林科学
Journal of the Hellenic Veterinary Medical Society Pub Date : 2023-10-18 DOI: 10.12681/jhvms.31023
B Dumlu, ŞC Bölükbaşı
{"title":"Effect of Solid State Fermentation with Whey on Nutrient Composition of Pomegranate Peel Supplemented Sunflower Meal","authors":"B Dumlu, ŞC Bölükbaşı","doi":"10.12681/jhvms.31023","DOIUrl":"https://doi.org/10.12681/jhvms.31023","url":null,"abstract":"The aim of the study is to improve the nutritional value of sunflower meal by solid state fermentation (SSF) using agricultural wastes such as whey and pomegranate peel. \u0000Experiment was conducted with six groups.  First group SM (sunflower meal) + TW (tap water), the second group SM + W (whey), the third group SM + W + 0.5% PP, the fourth group SM + W + 1% PP, the fifth group SM + W + 1.5% PP and the sixth group SM + W + 2% PP, respectively. The mixtures were prepared to complete 100 grams of sunflower meal with 0, 0,5, 1,0, 1,5 and 2,0 grams of pomegranate peel (PP), respectively. Each group consisted of eight replicates. Prepared mixtures were placed in 500 ml erlenmayers and 120 ml tap water was added to the mixing of first group and 120 ml whey was added in other groups and then mixed homogeneously. Four of the erlenmayers prepared for each group without being fermented were dried at room temperature. The remaining erlenmayers were fermented in 32°C±2 for 48 hours. Dry matter, crude protein, ether extract, crude ash, crude fiber, phytic acid ratios and phytase activities, antioxidant activities and yeast (Saccharomyces cerevisiae) numbers were determined in fermented and non-fermented feed samples. \u0000As a result of solid state fermentation with whey by adding pomegranate peel to sunflower meal, crude protein ratio, antioxidant activity and yeast count increased, and phytic acid ratio decreased. In conclusion, sunflower meal with functional properties was obtained. In addition, wastes such as whey and pomegranate peel were used as feed and environmental pollution was prevented. \u0000Keywords: Sunflower meal; pomegranate peel; whey; solid state fermentation; functional meal","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135942821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Occurrence and molecular characterization of Salmonella Typhimurium and Salmonella Enteritidis isolates from contaminated food samples from Palestine 巴勒斯坦受污染食品中鼠伤寒沙门菌和肠炎沙门菌分离株的发生及分子特征分析
4区 农林科学
Journal of the Hellenic Veterinary Medical Society Pub Date : 2023-10-18 DOI: 10.12681/jhvms.31088
G Adwan, S Abuseir, O Khreishi, A Hussein, N Khraim, M Abed Al-Daym
{"title":"Occurrence and molecular characterization of Salmonella Typhimurium and Salmonella Enteritidis isolates from contaminated food samples from Palestine","authors":"G Adwan, S Abuseir, O Khreishi, A Hussein, N Khraim, M Abed Al-Daym","doi":"10.12681/jhvms.31088","DOIUrl":"https://doi.org/10.12681/jhvms.31088","url":null,"abstract":"Salmonella is one of the most frequently isolated foodborne pathogens. It is of major public health concern worldwide. Poultry meat and eggs represent an important source of Salmonellae organism for consumer health. This study aimed to evaluate the occurrence of Salmonella enterica serotype Typhimurium and Enteritidis using multiplex PCR (mPCR) among isolates collected from the local market and to assess genetic relationships between isolates of S. Typhimurium, which was the only serotype isolated from the tested food samples. This was done using virulence factors profiling and fingerprint profiling by random amplified polymorphic DNA (RAPD-PCR) and repetitive sequence PCR (REP-PCR) using enterobacterial repetitive intergenic consensus (ERIC-PCR) and interspersed repetitive DNA sequence BOXAIR-PCR.&#x0D; The overall occurrence percentage of S. Typhimurium and S. Enteritidis out of 51 isolates was 54.9% and 0.0%, respectively. Only 13 out of 17 virulence genes were detected in these isolates. The occurrence of the detected virulence genes among these isolates was 100%, 50.0%,46.4%, 39.3%, 35.7%, 35.7%, 32.1%, 25.0%, 25.0%, 17.6%, 14.3%, 14.3%, 3.6% for invA, sopB, prgH, sitC, pefA, tolC, cdtB, msgA, sifA, iroN, spiA, ipfC and pagC, respectively. The remaining virulence genes were absent in all of the isolates. Based on the combination of the presence and absence of virulence genes, eight profiles were detected among these isolates, the most common genetic profile was V5 (each 32.1%). Based on this genetic profile at cut-off point 96.0%, both ERIC and BOX primers allowed for discrimination into 4 and 6 clusters or clones of 16 S. Typhimurium isolates, respectively. Results of PCR typing methods showed that, three strains clustered together using both ERIC-PCR and BOX-PCR typing methods and they had the same virulotype (V1), while other four strains also clustered together by both typing methods and had the same virulotype (V8).&#x0D; Contamination of food with Salmonellae especially with S. Typhimurium was high and indicated a bad microbiological quality of food. This emphasizes the need for rigorous public health and food safety methods to lower the human health hazard and risk associated with Salmonellae infection.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135942823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of low- and high-dose levothyroxine on the expression, protein level, and function of P-glycoprotein in mice 低、高剂量左甲状腺素对小鼠p -糖蛋白表达、蛋白水平及功能的影响
4区 农林科学
Journal of the Hellenic Veterinary Medical Society Pub Date : 2023-10-18 DOI: 10.12681/jhvms.31421
B Tras, K Uney, H Eser Faki, T Melik Parlak, Z Ozdemir Kutahya
{"title":"The effect of low- and high-dose levothyroxine on the expression, protein level, and function of P-glycoprotein in mice","authors":"B Tras, K Uney, H Eser Faki, T Melik Parlak, Z Ozdemir Kutahya","doi":"10.12681/jhvms.31421","DOIUrl":"https://doi.org/10.12681/jhvms.31421","url":null,"abstract":"The study was investigated the effect of different doses of levothyroxine on the mRNA expression, protein level and function of Pgp. Mice were divided into 6 groups as control, low dose levothyroxine, high dose levothyroxine, fexofenadine, low dose levothyroxine+fexofenadine and high dose levothyroxine+fexofenadine. Mice received levothyroxine at doses of 8 and 80 µg/kg daily for 21 days. Fexofenadine was administered at dose of 40 mg/kg at the 24 h following the last administration of levothyroxine. The mRNA levels and protein level of Pgp in liver and small intestine were determined by RT-PCR and western blot analysis, respectively. Plasma concentrations of fexofenadine were determined using HPLC. Levothyroxine at low and high doses caused an insignificant increase intestinal mRNA expression of mdr1a, while high dose levothyroxine+fexofenadine caused a significant increase. Levothyroxine caused a dose-dependent decrease in intestinal mRNA expression of mdr1b. In liver, levothyroxine caused a dose-dependent increase in the mRNA expression of mdr1a. Fexofenadine significantly reduced the effect of levothyroxine on mRNA expression of mdr1a in liver. Levothyroxine increased the protein level of Pgp in liver and decrease in intestines. Low dose levothyroxine significantly increased the plasma concentration of fexofenadine. The effects of levothyroxine on the mRNA expression of mdr1a and b in small intestine and liver and protein level of Pgp varied depending on the dose, tissue type, and fexofenadine administration.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135943705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Butorphanol with and without Medetomidine on Intraocular Pressure in Cats 布托啡诺加与不加美托咪定对猫眼压的影响
4区 农林科学
Journal of the Hellenic Veterinary Medical Society Pub Date : 2023-10-16 DOI: 10.12681/jhvms.26572
N Mohammadi, LE Yanmaz
{"title":"Effect of Butorphanol with and without Medetomidine on Intraocular Pressure in Cats","authors":"N Mohammadi, LE Yanmaz","doi":"10.12681/jhvms.26572","DOIUrl":"https://doi.org/10.12681/jhvms.26572","url":null,"abstract":"This study aimed to determine the effects of intramuscular administration of butorphanol with and without medetomidine on intraocular pressure (IOP) in cats. Sixteen clinically normal cats were used. Cats were randomly received two-treatment regimen. The first regimen was 0.2mg/kg butorphanol, and the second regimen was a mixture of 0.1mg/kg butorphanol and 0.05mg/kg medetomidine. IOP values were recorded before treatment (T0) and following the treatment at 10 (T10), 20 (T20), 30 (T30), and 40 min (T40) in both groups. Administration of butorphanol with and without medetomidine did not cause a statistically significant change in IOP values. The IOP did not change over time (p = 0.41). The mean values of IOP in butorphanol, and butorphanol-medetomidine groups were 20.00 ± 2.29 and 20.38 ± 2.35 mm Hg, respectively. In conclusion, intramuscular administration of butorphanol with or without medetomidine had no significant effect on IOP in cats.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136182115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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