M. Gholami-Ahangaran, A. Hjazi, HA Hussny, AA Amir, Abdulhussien Alazbjee, M. Karimi-Dehkordi, Haj Salehi, A. Ahmadi-Dastgerdi
{"title":"The effect of bromhexine on mucosal immune response against avian coronavirus infectious bronchitis vaccine in chickens","authors":"M. Gholami-Ahangaran, A. Hjazi, HA Hussny, AA Amir, Abdulhussien Alazbjee, M. Karimi-Dehkordi, Haj Salehi, A. Ahmadi-Dastgerdi","doi":"10.12681/jhvms.25477","DOIUrl":"https://doi.org/10.12681/jhvms.25477","url":null,"abstract":"Avian coronavirus infectious bronchitis (IB) is one of the most important and contagious disease in chickens, all over the world. Using of chemical compounds that improve the mucosal immune response to IB vaccine can increase resistance against the virus. To examine the effect of Bromhexine on the mucosal immune response against IB vaccine, 360 one-day broiler chicks were allocated to eight groups, randomly. Group one was the control group with no vaccine so that the group only used Bromhexine. Groups 2, 3, and 4 received IB vaccine for once, twice, and three times at the age of 5, 15, and 25 days, respectively. These groups received Bromhexine from 48 hrs before the vaccination to 24 hrs after receiving the vaccine in drinking water. Group five was the negative control group and did not receive Bromhexine and IB vaccine. Sampling in group five was at the age of 15 days. Groups six, seven, and eight were the main treatment control groups and received IB vaccine at the age of 5, 15, and 25 days without Bromhexine. The chickens were slaughtered 10 days after the vaccination and nose and trachea samples were collected and the mucus surface of respiratory was rinsed. Afterwards, specific immunoglobin A (IgA) level against IB vaccine in the respiratory mucus was measured through ELISA using chicken IgA specific antiglobulin. The results showed that the use of Bromhexine on the first vaccination had no effect on mucosal immune response. However, with the second and third vaccinations, antibody titter to IB vaccine was higher than chickens received vaccine without Bromhexin. It appears that using Bromhexine in booster IB vaccination can improve specific mucosal immune response.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139613125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Toward paratuberculosis control in animals: current updates and future perspectives","authors":"AA El-Sayed, M. Kamel, M. Zschoeck","doi":"10.12681/jhvms.27358","DOIUrl":"https://doi.org/10.12681/jhvms.27358","url":null,"abstract":"Paratuberculosis is a chronic non-curable disease, that affects domesticated and wild ruminants, pets and even humans. This disease causes significant economic losses worldwide. Many countries have implemented control programs to eradicate the disease. Such programs face great challenges due to the nature of the pathogen itself, the immune response, the method of pathogen shedding in susceptible animals, and the absence of accurate diagnostic tools, efficient vaccines, and curative medication. However, some control programs succeeded in eradicating the disease, others were less effective and achieved less success. The present review discusses the elements required in disease control protocols and highlights the importance of disease elimination. These control strategies include optimum application of management measures and proper use of combined diagnostic techniques to accurately identify MAP infected animals with high sensitivity and specificity. Nanotechnology has shown promising results in the diagnosis and control of paratuberculosis. All available vaccines do not completely protect the population, but they actually reduce the clinical signs, pathogen shedding and provoke cellular and humoral immune responses. Although the test and slaughter policy of paratuberculosis is considered an effective way for its control, several obstacles hinder its application.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139612431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"sTREM-1, MR-Pro-Adrenomedullin, clinical biochemistry and hematology in calves with neonatal sepsis","authors":"M. Sezer, G. Gökce","doi":"10.12681/jhvms.31046","DOIUrl":"https://doi.org/10.12681/jhvms.31046","url":null,"abstract":"This study aimed to determine the changes in biochemical and hematological parameters and the expression of soluble triggering receptor expressed on myeloid cells-1 (sTREM 1) and mid-regional-pro-adrenomedullin (MR-Pro-ADM) on myeloid cells during treatment in calves with neonatal sepsis. The study group comprised 21 calves, aged 1–16 days, that fit the sepsis criteria. The control group comprised 10 healthy calves of the same age. The clinical examinations of the calves in the study group were performed at certain hours before the treatment (hour 0) and during the treatment (12th, 24th, 48th, and 72nd hours). The blood samples were taken from the vena jugularis before and during treatment from the sick calves and once from the healthy calves. The sTREM-1 levels before treatment (0 h), during treatment (12th, 24th, 48th, and 72nd hours) and control group was follows 59.17±2.58 ng/L, 57.07±1.65 ng/L, 57.27±2.07 ng/L, 56.64±1.97 ng/L, 57.58±2.18 ng/L and 75.60±2.57 ng/L,39 respectively. The MR-Pro-ADM levels before treatment (0 h), during treatment (12th, 24th, 48th, and 72nd hours) and control group was follows; 117.88±7.75 ng/L, 113.63±5.18 ng/L, 109.97±5.25 ng/L, 114.50±5.35 ng/L, 111,84±5.63 ng/L,65.31±2.81 ng/L, respectively. The serum MR-Pro-ADM levels in calves with sepsis were significantly higher than those in the control group before and during treatment (P < 0.05). Also, the serum sTREM-1 level was significantly lower before and after treatment in the sepsis group compared with the control group (P < 0.05). MR-Pro-ADM and sTREM-1 were more important, especially in terms of diagnosis in evaluating calves with neonatal sepsis.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139613724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Moosavian, H. Esmaeilzadeh, M. Fazli, CA Thompson, A. Farajzadeh
{"title":"Presumptive injection-site sarcoma in a white tiger (Panthera tigris tigris): A case report","authors":"H. Moosavian, H. Esmaeilzadeh, M. Fazli, CA Thompson, A. Farajzadeh","doi":"10.12681/jhvms.30244","DOIUrl":"https://doi.org/10.12681/jhvms.30244","url":null,"abstract":"A 6-year-old female White Tiger (Panthera Tigris Tigris) was presented with a history of a recurrent ulcerating mass on the right lumbar wall which initially, was removed by surgery one year before presentation. Cytological and histological findings were consistent with a cutaneous injection site sarcomas. Immunohistochemistry results revealed that the neoplastic cells were strongly positive for vimentin and weakly positive for alpha-smooth muscle actin (SMA), but negative for Cytokeratin AE1/AE3, s100, and Desmin. Positive staining for vimentin and SMA is consistent with myofibroblast reactivity and reflects a continuous inflammatory response seen in feline injection-site sarcomas. Feline injection-site sarcomas are the most serious adverse effect following injection of the vaccine or some other pharmaceutical substances and should be considered in the differential diagnosis of skin swelling in injection sites in the Felidae family. Limited data are available for these tumors in animals outside of domesticated cats. To the best of our knowledge, there exists no available published information on the injection-site sarcoma in tigers. Similar to domestic felids, this case highlights the importance of considerations and management options in vaccination schedules for the reduction of sarcoma risk in wild felids.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139614346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of GnRH and hCG Treatment Following a Short-Term Estrus Synchronization Protocol on Ovulation and the Fertility in Merino Ewes, During the Breeding Season","authors":"I. Dogan, MB Toker, A. Aktar, MM Yilmaz","doi":"10.12681/jhvms.31541","DOIUrl":"https://doi.org/10.12681/jhvms.31541","url":null,"abstract":"This study aimed to compare the effect of hCG or GnRH administered 36 h following a short-term estrus synchronization treatment on the ovulation time and pregnancy rates of Merino ewes during the breeding season. The estrus cycles of ewes were synchronized with an intravaginal sponge containing 60 mg of medroxyprogesterone acetate for 6 days, and an injection of 400 IU of eCG and 125 µg of d-cloprostenol 24 h before sponge removal. Thirty-six h after the sponge removal, ewes were injected intramuscularly either 1 ml of physiological saline solution (control-group; n=14), 100 IU of hCG (hCG-group; n=14) or 0.004 mg of buserelin acetate (GnRH-group; n=14). The estrus behavior was observed using teaser rams and the ovulation time was monitored using transrectal ultrasonography twice daily for 96 h after the sponge removal. Ewes in estrus were allowed a single mating using fertile rams. Estrus response was higher (P<0.05) in the control group (92.86%) than in the GnRH group (50.00%). The interval from sponge removal to ovulation and from hCG to ovulation were shorter in the control group than in the hCG group (70.55, 80.83 h; 34.55, 44.83 h, respectively, P<0.05). In conclusion, administration of hCG or GnRH at 36 h following a short-term estrus synchronization protocol did not affect estrus behaviors, ovarian findings and pregnancy rates in ewes during the breeding season.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139615178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Abnormal twins: dog and cat","authors":"A. Pourlis, A. Tsingotjidou","doi":"10.12681/jhvms.35089","DOIUrl":"https://doi.org/10.12681/jhvms.35089","url":null,"abstract":"A survey of congenital malformations in dog and cat relating to their abnormal embryonic twinning was carried out. According to the degree, sites and angle of fusion, they have various external variation and are classified as free asymmetric, conjoined symmetric or asymmetric twins and unequal conjoined twins (heteropagus or parasitic twins). This manuscript aims to describe and summarize these defects. Among the recorded duplications in dog, a number of common defects relates to cephalothoracopagus phenotype whereas some cases of caudal duplication or parasitic twins have been surveyed. Among the recorded publications in cat, a number of craniofacial duplications have been encountered, whereas, some cases of thoracopagus phenotype have also been described. The pathogenetic mechanisms of this condition, reported in veterinary practice, are discussed. The importance in clinical practice lies in the fact that abnormal embryonic twinning is commonly associated with dystocia. Treatment of the diseased animals is also of veterinary practice consideration. The manuscript finally introduces a framework of an essential national registry for the malformed companion animals.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139616094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Controlling the photoperiod to raise the melatonin content of sheep milk. Photoperiod control and milk melatonin content","authors":"M. Keskin, S. Gül, İ. Karaaslan, A. Yakan","doi":"10.12681/jhvms.31879","DOIUrl":"https://doi.org/10.12681/jhvms.31879","url":null,"abstract":"The aim of this study was to determine the effect of changing photoperiod time on the melatonin content of sheep milk. The animal material of the study consisted of 40 heads of Awassi sheep that gave birth in May 2021. The sheep were given roughage containing dry matter with 2.5% of their body weight and concentrated feed (2400-2600 kcal ME and 15-16% Crude Protein) as the 40% of their milk yield each day during the lactation period. The experimental ewes were randomly assigned into two groups with 20 animals in each. While the control group sheep were managed with normal day-time length during the lactation, the photoperiod restriction group were kept in the shed with 16 hours of darkness and 8 hours of daylight for 2 months. At the end of the two months, ewes of the both groups were managed in normal daylight until the end of lactation period. During the photoperiod restriction term, milk samples were taken at an interval of 15 days, while the milk composition was determined by a milk analyser and melatonin contents were determined by using LC MS/MS. In addition, lactation milk yield and lactation periods were estimated for all sheep. Statistical evaluation of the obtained data was done by using the SPSS package program. At the end of the study, the application of the extended dark period was found to be affected milk yield (P<0.05) as well as melatonin amount in the milk (P<0.001). Significantly higher melatonin amount was observed in the milk of sheep kept in a long-term dark environment compared to those exposed to normal photoperiod (11.1±7.24 pg/ml vs 6.1±4.55 pg/ml; P<0.001). As a result, it was determined that the prolonged dark period during a day increases the milk melatonin content. It can be also stated that the sheep's milk to be produced with this application could be a product that could take place in the functional food market.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139616101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ö. Hazman, ME Göçeri, I. Fıçıcı, B. Demirer, L. Aksoy
{"title":"The effect of Melissa officinalis on diet-induced hyperlipidemia, hypercholesterolemia, oxidative stress and inflammation","authors":"Ö. Hazman, ME Göçeri, I. Fıçıcı, B. Demirer, L. Aksoy","doi":"10.12681/jhvms.30235","DOIUrl":"https://doi.org/10.12681/jhvms.30235","url":null,"abstract":"The Lamiaceae family is an essential medicinal plant family. Melissa officinalis L. is a well-known medicinal plant of Lamiaceae. It was aimed to investigate the effects of Melissa officinalis infusion given to rats with hyperlipidemia and hypercholesterolemia in this study. It is seen that the high-fat diet (HFD) group causes weight gain from the third week. VLDL, LDL, HDL and cholesterol levels were found statistically significant differences (p˂0.05), fed a cholesterol-enriched high-fat diet group (HFD) compared to the control. It is seen that the cholesterol level of the high-fat diet+Melissa officinalis infusion (HFD+MOI) group was statistically significantly (p˂0.05). lower than the HFD group. In addition, the group in which Melissa officinalis was applied had positive effects on the antioxidant system and reduced the total oxidant system. The fact that the IFN-γ level of the HFD group was higher than the control indicates that the diet may have an inflammatory effect. As a result, HFD application with cholesterol caused hyperlipidemia and hypercholesterolemia in rats. The cholesterol-lowering effect of Melissa officinalis is observed. Remarkably, Melissa officinalis did not alter serum calcium levels while lowering cholesterol. In addition, it can said that HFD has a strengthening effect on the antioxidant system on oxidative stress.","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139615510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ultrastructure of mice testes affected by orally administered crocin of crocus sativus","authors":"Arh Al-Fartwsy, AA Mohammadpour, A. Parham","doi":"10.12681/jhvms.30777","DOIUrl":"https://doi.org/10.12681/jhvms.30777","url":null,"abstract":"In this study, thirty-two adult male mice were divided into four groups control and experimental treatment groups received crocin at 4, 20, and 100 mg/kg daily for six weeks. In results, testosterone level was increased significantly from 18.5 ng/dl in the control to 49.3 ng/dl in group received crocin at 20 mg/kg. The regular features of seminiferous tubules with increased Sertoli cells have been observed in the 20mg/kg crocin group. \u0000 In the group received crocin at 100 mg/kg, many of the seminiferous tubules were atrophic, spermatogenic cells were discontinuous in the same areas while the space among them has been increased, and fewer interstitial tissue between seminiferous tubules were observed. The changes were markedly advanced by increasing the number of atrophied seminiferous tubules. \u0000Electron microscopic results showed the large mitochondria with normal crista and dilated rough endoplasmic reticulum in spermatid cells of the 20 mg/kg crocin group. \u0000In the 100 mg/kg crocin group, most of the cell cytoplasm showed vacuolated mitochondria with patchy matrix and distorted vision cristae, with many lysosomes, lipid droplets and vacuoles. Some sperms had abnormal swollen nuclear and irregular wavy acrosomal system. \u0000The results of the present study showed that crocin at 20 mg/kg has a protective effect on the germinal epithelium of seminiferous tubules while at 100 mg/kg had destructive effects on the histological structure of the treated mice testicles. \u0000In conclusion, treating healthy male mice with crocin for six weeks increased the testosterone concentration while at 100 mg/kg damaged the testicle’s structure","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139615745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Cetin, E. Cetin, D. Karakci, E. Ercetin, Bugdayci Kırmızı, DY Yeşilbağ, O. B. Kırmızı, D. Yesilbag, D. Yesilbag
{"title":"The effects of rosemary essential oil supplementation on growth performance, rumen flora and antioxidant blood parameters in growing Merino lambs","authors":"I. Cetin, E. Cetin, D. Karakci, E. Ercetin, Bugdayci Kırmızı, DY Yeşilbağ, O. B. Kırmızı, D. Yesilbag, D. Yesilbag","doi":"10.12681/jhvms.31728","DOIUrl":"https://doi.org/10.12681/jhvms.31728","url":null,"abstract":"Thirty-two male growing Merino lambs (78 ± 5 days of age) were used in a 45-day randomized complete block desing to determine the effects of rosemary essential oil (REO) on the growth performance, rumen fermentation, rumen protozoa population, plasma antioxidant enzyme parameters and fecal microbiology. The control group was fed a diet consisting of concentrate and alfalfa hay. The lambs in control group did not receive REO whereas each lamb in experimental groups supplemented with 250 mg/d (R250), 500 mg/d (R500) and 750 mg/d (R750) REO throughout the study. There weren’t statistically differences (P>0.05) in average initial weight, average final weight, live weight gain, average daily gain, daily feed intake, daily dry matter intake and feed conversion ratio between the control and experimental groups. There were also no differences (P>0.05) in the rumen pH, fecal pH, NH3N and rumen volatile fatty acid (VFA) profile of the control and experimental groups. At the end of the study, although the plasma superoxide dismutase (SOD) value increased significantly (P<0.05) in the experimental groups (R500 and R750), glutathione peroxidase (GPX) and catalase values did not differ between REO groups and control. In addition, the fecal microorganism profile did not differ statistically (P>0.05) between the control and experimental groups. The results of this study show that the addition of REO to Merino lamb rations is more effective on antioxidant parameters than on performance parameters and can be used as a natural antioxidant product","PeriodicalId":17314,"journal":{"name":"Journal of the Hellenic Veterinary Medical Society","volume":null,"pages":null},"PeriodicalIF":0.4,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139616102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}