{"title":"BLASTOCYSTIS SUBTYPE 3 AMONG ADOLESCENTS WITH GASTROINTESTINAL SYMPTOMS IN FAYOUM GOVERNORATE, EGYPT","authors":"Faten Mohamed, Kholoud Khalil","doi":"10.21608/jesp.2023.331732","DOIUrl":"https://doi.org/10.21608/jesp.2023.331732","url":null,"abstract":"Blastocystis is a common polymorphic protozoan infecting human intestine with a zoonotic potential. Despite the unclear pathogenicity, its existence may be linked to a wide range of gastrointestinal disorders. The present cross sectional study was conducted to analyze Blasto-cystis subtypes among 110 adolescents suffering from disturbing GIT symptoms in stool samples. Positive samples (21 (19.1%)) by microscopy were subjected to DNA isolation and sub-typed by Sequence Tagged Site (STS)-PCR analysis, which successfully subtyped 10 (62.5%) of 16 isolates. The dominant Blastocystis subtype was ST3 in 6 (40%) samples followed by 3 (30%) for ST2, but one sample (10%) was amplified for ST1. The results revealed significant association between nausea and vomiting and Blastocystis infection. However, there was no difference between the Blastocystis subtypes and demo-clinical characteristics.","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":"43 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139191579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"INTRANASAL DEXMEDETOMIDINE 2μG/KG VERSUS 3μG/KG ON PARENT SEPARATION IN DENTAL SURGERY: A RANDOMIZED CLINICAL TRIAL","authors":"Wael Hussein","doi":"10.21608/jesp.2023.331738","DOIUrl":"https://doi.org/10.21608/jesp.2023.331738","url":null,"abstract":"","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":"27 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139193524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ASSESSMENT OF NURSES’ KNOWLEDGE AND PRACTICE TO GASTROINTESTINAL ENDOSCOPY CARE TO ENSURE SAFETY IN A MILITARY HOSPITAL","authors":"M. Elnagar, S. Mohamed, Eman ABDEL FADIL","doi":"10.21608/jesp.2023.331740","DOIUrl":"https://doi.org/10.21608/jesp.2023.331740","url":null,"abstract":"","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":"19 45","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139193862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marwa Naguib, Azza El Hamshary, Ibrahim Baoumi, Azza M. Marei, Maha Fawzy, A. EL-GHANNAM
{"title":"THERAPEUTIC EFFICACY OF LACTOFERRIN LOADED ON NANO-PARTICLES IN TRICHINELLA SPIRALIS INFECTION IN MICE","authors":"Marwa Naguib, Azza El Hamshary, Ibrahim Baoumi, Azza M. Marei, Maha Fawzy, A. EL-GHANNAM","doi":"10.21608/jesp.2023.331737","DOIUrl":"https://doi.org/10.21608/jesp.2023.331737","url":null,"abstract":"Trichinellosis is a worldwide risky parasitic zoonosis caused by a nematode of the genus Trichinella . Anthelmintic such as albendazole and mebendazole commonly used to treat trichi-nellosis are badly water soluble and exhibit unfavorable bioavailability after oral administration increasing need for effective and safe drug. This study evaluated the therapeutic efficacy of lactoferrin loaded on silver nanoparticles in treating T. spiralis infection in mice by parasito-logical, immunological, and histopathological parameters. Forty albino mice were classified into 8 groups of 5 mice. G1: Non-infected non-treated (normal), (G2): Infected non-treated (infected control). G3: Infected and treated with albendazole, G4: Infected and treated with lactoferrin, G5: Infected and treated with silver nanoparticles, G6: Infected and treated with lactoferrin loaded on silver nanoparticles, G7: Infected and treated with albendazole in combination with lactoferrin, & G8: Infected and treated with albendazole in combination with lactoferrin loaded on silver nanoparticles. The results revealed a significant decline in all treated mice with the highest reduction rate of T. spiralis adult count in G8 treated with a combination of albendazole and lactoferrin loaded on silver nanoparticles (98.59%), followed by G7 treated with albendazole and lacto-ferrin (95.77%), G3 treated with albendazole (90.38%), G6 treated with lactoferrin loaded on silver nanoparticles (84.27%), G4 treated with lactoferrin (59.86%), and lowest reduction rate (22.3%) was in G5 treated with nanoparticles. Also, there was a significant decline in TNF-& IL-10 levels and an improvement of the intestinal histopathological changes in mice treated with a combination of albendazole and lactoferrin loaded on silver nanoparticles.","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":"25 1-2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139188698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"HUMAN LICE INFESTATION, DIAGNOSIS AND TREATMENT: A MINI-REVIEW","authors":"Tosson Morsy, Areej AL-GHABBAN","doi":"10.21608/jesp.2023.331736","DOIUrl":"https://doi.org/10.21608/jesp.2023.331736","url":null,"abstract":"","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":"24 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139188953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"SOIL-TRANSMITTED HELMINTHS CO-INFECTED WITH SCHISTOSOMA IN FLOOD-PLAIN COMMUNITIES, SOUTHERN, NIGERIA","authors":"E. Ito, A. Egwunyenga","doi":"10.21608/jesp.2023.331745","DOIUrl":"https://doi.org/10.21608/jesp.2023.331745","url":null,"abstract":"This study evaluated the soil transmitted helminths (STH) and Schistosoma co-infection and its epidemiological factors in flood subjected communities. Fecal samples from 672 consented individuals between 5-24 years in Aviara, Igbide, Otor-Owhe and Owhelogbo community were examined using Kato-Katz technique and questionnaires. Of 672 samples, 434(64.58%) were infected with STHs and Schistosoma mansoni co-infections . The parasites in a descending were Ascaris lumbricoides (56.40%), Trichuris trichiura (54.32%), hookworm (38.69%) and S. mansoni (7.59%), with significant differences. Female 325 (48.36%) were more infected than male 317(47.17%). But, among all communities, there was no significant difference in gender prevalence. The general prevalence correlated to infection intensity. Ages of 5-9 years old were more vulnerable to STHs infections. Helminthes was not significant ( p<0.05 ) with age of children except A. lumbricoides which significantly different with age (t = 5.16, 95% CI = 5.44 and 60.12, P = 0.036). Age-community prevalence accounted for <0.1% of total variance (P = 0.859, F = 0.033). Effect of toilet type, toilet paper and washing of hands after defe-cation was significant (P<0.05). ANOVA showed a strong significant impact ( p <0.005) in maternal educational and water source in each community. This study revealed that STH and intestinal schistosomiasis were highly prevalent among children of 5-14 years. There is need for prompt wash and mass drug administration (MDA) to reduce prevalence/intensity and morbidity in the study area","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":"25 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139190838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Rashed, Mona E. Nasr, Atef H. ABD EL HAMID, Nagwa S. M. Aly, A. Barakat, Kawkab A. Ahmed, Safaa H. Youssif, Ghada Omar
{"title":"ASSESMENT OF THE THERAPUTIC EFECT OF LATE AZITHROMYCIN TREATMENT ON CHRONIC MURINE TOXOPLASMOSIS","authors":"G. Rashed, Mona E. Nasr, Atef H. ABD EL HAMID, Nagwa S. M. Aly, A. Barakat, Kawkab A. Ahmed, Safaa H. Youssif, Ghada Omar","doi":"10.21608/jesp.2023.331734","DOIUrl":"https://doi.org/10.21608/jesp.2023.331734","url":null,"abstract":"Toxoplasmosis is a widespread, neglected disease with significant morbidity and mortality. The current study aimed to investigate the potential effectiveness of azithromycin against the cystogenic Me 49 nonvirulent strain of Toxoplasma gondii after the 6 th week post-infection (PI) in chronically infected mice. Fifty laboratory-bred female Swiss albino mice were divided into five groups: G1: Non-Infected, G2: Infected non-treated, G3: Infected and treated by azithromycin 200 mg/kg after the 6 th week PI for 3 days, G4: Infected and treated by azithromycin 200 mg/kg after the 6 th week PI for 10 days and G5: Infected and treated by spiramycin 200 mg/kg after the 6 th week PI for 2 weeks, brain cysts number, size and histopathological changes were evaluated after 2 months. The results showed that treating mice after the 6 th week post-infection with azithromycin for 10 days reduced number and size of brain cysts by 64% & 30%, respectively, but without significant difference as compared with spiramycin-treated ones. Azithromycin treatment for 3 days achieved a greater reduction (34%) in brain cyst size than the group treated with azithromycin for 10 days. But, it caused the least reduction in brain cyst number (10%). Azithromycin treatment for 10 days improved the histopathological changes in cerebral toxoplasmosis.","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":"49 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139192988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"EFFECTS OF INTRAMUSCULAR INJECTION OF PLATELET-RICH PLASMA ON LARVAL BURDEN OF TRICHINELLA SPIRALIS IN EXPERIMENTALLY INFECTED MICE","authors":"Fatma Shalaan, Tahany AL-ATTAR, Shereen Mahmoud, Huda ABD-ELHAFIZ, Yousra AL-GHALBAN, M. Faheem","doi":"10.21608/jesp.2023.331741","DOIUrl":"https://doi.org/10.21608/jesp.2023.331741","url":null,"abstract":"Trichinellosis (= trichinosis) is widespread food-borne zoonotic parasitosis caused by the nematode Trichinella spiralis triggering skeletal muscle injury. This study identified the therapeutic effects of platelet-rich plasma (PRP) as a safe choice in muscular phase of mice experimental infected with T. spiralis . Ninety male mice were divided into three main groups, GI: Negative con-trol, GII: Positive control and GIII: Trichinella infected PRP treated. All mice were sacrificed on 14 th day post infection (initiation of muscle cell invasion), on 21 st day (early stage of nurse cell formation), on 28 th day (formation of most nurse cells was completed), and on 35 th day PI (convalescent period). The parasitological examination showed a significant reduction in the number of larvae detected in the infected PRP-treated ones 35 th PI. Histopathological and immunohistochemical of vascular endothelial growth factor, caspase 3 analyses coincided with parasitological findings, on 35 th day PI as well. There was a significant improvement in the in-flammatory cells with fewer muscle fibers degradation, indicating that PRP injection in trichinellosis muscular phase improved inflammatory reaction and reduce T. spiralis larval burden.","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":"21 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139188745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mona Ali, N. El-Dib, Marwa Ghieth, Heba Ahmed, Mahmoud Hodeib, H. El-Askary, Samah S. Abdel Gawad
{"title":"CAPILLARIA PHILIPPINENSIS IDENTIFIED IN DIARRHEIC PATIENTS FROM BENI-SUEF UNIVERSITY HOSPITALS, EGYPT","authors":"Mona Ali, N. El-Dib, Marwa Ghieth, Heba Ahmed, Mahmoud Hodeib, H. El-Askary, Samah S. Abdel Gawad","doi":"10.21608/jesp.2023.331742","DOIUrl":"https://doi.org/10.21608/jesp.2023.331742","url":null,"abstract":"Capillariasis philippinensis is a debilitating nematode zoonotic parasite affecting all ages. Patients usually suffered from borborygmus, abdominal pain, chronic diarrhea, protein loss, anemia, and weight loss, as well as decrease in electrolyte levels of potassium (K), sodium (Na) and albumin due to enteropathy. The microscopic examinations showed that 39/450 (8.6%) were C . philippinensis patients. The different parasitic stages were also examined by SEM. Biochemical estimation of albumin, Na and K levels were assessed as well as blood examination for hemoglobin level, total leucocyte count and eosinophila. Parasitic males, females and egg stages were identified by light microscope and SEM. Only anemia in children was significant as compared to other patients ( P <0.05). Most cases were associated with lowered albumin","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":"68 3-4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139189785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A MINI-REVIEW ON LARGE LOCAL REACTIONS TO MOSQUITO BITES OR SKEETER SYNDROME TO SALIVA AND OTHERS' SALIVA","authors":"Tosson Morsy, Tarek KADER SALLAM, Suzan EL-SHAHAT","doi":"10.21608/jesp.2023.331730","DOIUrl":"https://doi.org/10.21608/jesp.2023.331730","url":null,"abstract":"No doubt, blood-sucking insect-vectors are rapidly increasing worldwide with climatic chang-es. Mosquitoes spread infective stages through bites, such as viruses, bacteria","PeriodicalId":17289,"journal":{"name":"Journal of the Egyptian Society of Parasitology","volume":"57 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139192937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}