Journal of submicroscopic cytology and pathology最新文献_第6页

An ultrastructural study of sperm of the genus Bufo (Amphibia, Anura, Bufonidae). 蟾蜍属(两栖纲，无尾目，蟾蜍科)精子的超微结构研究。

Journal of submicroscopic cytology and pathology Pub Date : 2004-07-01

S V Braz, A P Fernandes, S N Báo

引用次数: 0

Evaluation of the in vitro effect of albendazole, metronidazole and nitazoxanide on viability and structure of Giardia lamblia cysts. 阿苯达唑、甲硝唑和硝唑尼特对兰氏贾第鞭毛虫囊体活力和结构影响的体外评价。

Journal of submicroscopic cytology and pathology Pub Date : 2004-07-01

R Bernal-Redondo, L G Martínez-Méndez, A Mendoza-Chavez, D Velasco-Perales, B Chavez-Munguia

引用次数: 0

Morphological aspects of muscle regeneration in the Nile tilapia (Oreochromis niloticus). 尼罗罗非鱼肌肉再生的形态学方面。

Journal of submicroscopic cytology and pathology Pub Date : 2004-07-01

A A P Camargo, R F Carvalho, V Dal-Pai, C H Pellizzon, M Dal-Pai-Silva

引用次数: 0

A new antigen retrieval method using citraconic anhydride for immunoelectron microscopy: localization of surfactant pro-protein C (proSP-C) in the type II alveolar epithelial cells. 免疫电镜下用柠檬酸酐提取抗原的新方法:在II型肺泡上皮细胞中定位表面活性剂前蛋白C (proc -C)。

Journal of submicroscopic cytology and pathology Pub Date : 2004-07-01

W Dai, S Sato, M Ishizaki, K Wakamatsu, S Namimatsu, Y Sugisaki, M Ghazizadeh

{"title":"A new antigen retrieval method using citraconic anhydride for immunoelectron microscopy: localization of surfactant pro-protein C (proSP-C) in the type II alveolar epithelial cells.","authors":"W Dai, S Sato, M Ishizaki, K Wakamatsu, S Namimatsu, Y Sugisaki, M Ghazizadeh","doi":"","DOIUrl":"","url":null,"abstract":"In the attempt to develop a pre-embedding immunoelectron microscopic method with improved ultrastructral morphology, we examined an antigen retrieval (AR) method using citraconic anhydride, and compared the effects of glutaraldehyde fixation with routine paraformaldehyde fixation in the pre-embedding immunoelectron microscopic method with reference to the localization of surfactant-associated pro-protein C (proSP-C) in the lung. The glutaraldehyde-fixed tissues were immunostained after AR in 0.05% citraconic anhydride solution, pH 7.4, at 98 degrees C for 60 min. In routine pre-embedding immunoelectron microscopic method using paraformaldehyde fixation, proSP-C positive products were distributed sporadically in the type II alveolar epithelial cells. In glutaraldehyde-fixed tissues without the AR method, proSP-C products were not detected, however after AR in citraconic anhydride proSP-C positive products were distributed specifically, in rough endoplasmic reticulum membranes, Golgi complex membranes, multivesicular bodies and osmiophilic lamellar bodies. The positive proSP-C products also showed lattice-like structures in the alveoli. Thus, the present AR method provides successful pre-embedding immunoelectron microscopy of glutaraldehyde-fixed tissues.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":"36 3-4","pages":"219-24"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25294215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acellular dermal tissue study: an ultrastructural evaluation of human and porcine derived tissues in a rat model. 脱细胞真皮组织研究:大鼠模型中人类和猪源性组织的超微结构评价。

Journal of submicroscopic cytology and pathology Pub Date : 2004-07-01

E Hoyama, S A Schellini, E A Gregório, R Rossa

{"title":"Acellular dermal tissue study: an ultrastructural evaluation of human and porcine derived tissues in a rat model.","authors":"E Hoyama, S A Schellini, E A Gregório, R Rossa","doi":"","DOIUrl":"","url":null,"abstract":"The purpose of this study was to evaluate the host response of a human and a porcine derived acellular dermal tissue (ADT) implanted in the subcutaneous tissue of a rat model. Two subcutaneous pockets were surgically created along the dorsal midline of 25 rats (5 rats/group). The human ADT was placed superiorly and the porcine ADT, inferiorly. The animals were sacrificed at 07, 15, 30, 60 and 180 postoperative days (PO) and the ADTs and surrounding soft tissues were assessed for ultrastructural evaluation by transmission electron microscopy. The ultrastructural findings were similar in both materials. Normal collagen and elastic fibers bundles were observed during all experimental moments, as well as macrophages presenting cytoplasmic enlargements digesting cellular portions after 15 PO. From 30 until 180 PO, vacuolar structures filled with an amorphous, electron-transparent substance, were present inside and outside the fibroblasts. Both human and porcine ADT showed similar pattern of ultrastructural response when implanted in the subcutaneous tissue of rats. The porcine ADT appears as a good alternative to be used as a biomaterial.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":"36 3-4","pages":"305-8"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25121424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathobiology of familial hypercholesterolemic atherosclerosis. 家族性高胆固醇血症性动脉粥样硬化的病理生物学。

Journal of submicroscopic cytology and pathology Pub Date : 2004-07-01

G Aliev, R J Castellani, R B Petersen, G Burnstock, G Perry, M A Smith

{"title":"Pathobiology of familial hypercholesterolemic atherosclerosis.","authors":"G Aliev, R J Castellani, R B Petersen, G Burnstock, G Perry, M A Smith","doi":"","DOIUrl":"","url":null,"abstract":"Many factors play a role in the development of atherosclerotic lesions. One of the leading risk factors for development of atherosclerosis is familial hypercholesterolemia (FH). FH is a genetic disease characterized by a deficiency, and/or mutation, of receptors for low density lipoprotein (LDL) on the plasmalemma of endothelial cells (EC), a high level of low density lipoprotein in the plasma, and early, spontaneous development of atherosclerosis and skin xanthoma. In this review we describe Watanabe heritable hyperlipidemic (WHHL) rabbits, which represent such an animal model for human FH. This strain of the rabbits is characterized by a genetic deficiency or mutation of functional LDL receptors and develops severe atherosclerosis, which is pathologically similar to familial homozygous hyperlipidemic patients. The most completely characterized animal model is the Watanabe rabbit, a model of homozygous and heterozygous type IIa hypercholesterolemia related to an LDL receptor deficiency. Additional manipulation such as aortic injury in this rabbit model induces the development of atherosclerotic lesions that are structurally similar to those found in humans. Thus, this model of hypercholesterolemia fulfils the above criteria set, i.e. it is able to provide new insights for a better understanding of the pathogenesis of atherosclerosis and for testing new treatment strategies.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":"36 3-4","pages":"225-40"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25294216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lysosome abnormalities and lipofucsin content of nerve cells of oedematous human cerebral cortex. 人大脑皮质水肿神经细胞溶酶体异常及脂褐素含量。

Journal of submicroscopic cytology and pathology Pub Date : 2004-07-01

O J Castejón

{"title":"Lysosome abnormalities and lipofucsin content of nerve cells of oedematous human cerebral cortex.","authors":"O J Castejón","doi":"","DOIUrl":"","url":null,"abstract":"Lysosome alterations and lipofucsin content of nerve cells, capillary endothelial cells and pericytes were examined in the anoxic-ischaemic brain parenchyma of thirty two patients with congenital hydrocephalus, complicated brain traumatic injuries, brain tumours and vascular anomalies. Cortical biopsies of frontal, parietal and temporal cortex were processed for transmission electron microscopy. In oedematous non pyramidal and pyramidal nerve cells, lysosomes showed fragmentation of their limiting membranes and an associated dense granulation. Areas of cytoplasmic focal necrosis were observed surrounding the lysosomes. Lipofucsin granules were also observed in neonate and infant patients with congenital hydrocephalus, suggesting that lipofucsin formation is a life span process. Lysosomes coexisting with an increased amount of lipofucsin granules were observed in young and adult patients with brain trauma, tumours and vascular anomalies. Phagocytic astrocytes and activated oligodendroglial cells showed the overall spectrum of an altered endosomal/lysosomal system. Lipofucsin granules and multivesicular bodies also were distinguished in endothelial and pericyte cells. The role of released and activated lysosomal enzymes is discussed in relation with the cytoplasmatic focal necrosis of nerve cells and the genesis of moderate and severe oedema.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":"36 3-4","pages":"263-71"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25294220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sperm fine structure of the pikeperch, Sander lucioperca (Percidae, Teleostei). 刺鲈科刺鲈精的精细结构。

Journal of submicroscopic cytology and pathology Pub Date : 2004-07-01

F Lahnsteiner, N Mansour

引用次数: 0

Light microscopical and ultrastructural studies on the vas deferens of the lizard Mabuya carinata. 壁虎输精管的光镜和超微结构研究。

Journal of submicroscopic cytology and pathology Pub Date : 2004-07-01

I Aranha, M Bhagya, H N Yajurvedi, B K Chandrashekar Sagar

{"title":"Light microscopical and ultrastructural studies on the vas deferens of the lizard Mabuya carinata.","authors":"I Aranha, M Bhagya, H N Yajurvedi, B K Chandrashekar Sagar","doi":"","DOIUrl":"","url":null,"abstract":"Adult male lizards (Mabuya carinata) were studied during breeding and non breeding seasons to determine the regional and seasonal differences if any in the vas deferens and to compare ultrastructural features of luminal epithelial cells with those of endotherms. The vas deferens of the lizard is a convoluted tube extending from the epididymis to the hemipenis passing over the kidney. Based on morphometric data of luminal diameter and epithelial cell height three distinct regions viz; proximal, middle and distal regions were identified in the vas deferens. The epithelium is surrounded by a thin layer of lamina propria, many layers of circular smooth muscle fibers and an outer layer of visceral pleuro peritoneum. Based on cell and nuclear morphology and ultrastructure, five different cell types viz; principal cell, basal cell, mitochondria rich cell, halo cell and narrow cell were identified in the epithelium during both breeding and non breeding season. Principal cells and basal cells were more abundant in both seasons. The types of luminal epithelial cells of vas deferens of M. carinata and their ultrastructural features are similar to those of mammals. Further, vas deferens of M. carinata differs from mammals in having only circular smooth muscles in contrast to circular and longitudinal muscles of mammalian vas deferens. To the best of our knowledge this is the first report describing cell types of vas deferens, their ultrastructure and ultrastructural seasonal variations in reptiles.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":"36 3-4","pages":"247-56"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25294218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contrast enhancement of the retinal image and image distortion associated with macular degeneration. 视网膜图像的对比度增强和与黄斑变性相关的图像失真。

Journal of submicroscopic cytology and pathology Pub Date : 2004-07-01

F S Sjöstrand

{"title":"Contrast enhancement of the retinal image and image distortion associated with macular degeneration.","authors":"F S Sjöstrand","doi":"","DOIUrl":"","url":null,"abstract":"An analysis of image distortion in connection with macular degeneration revealed that seeing objects requires brightness contrast enhancement. Thus macular degeneration changes neural interaction controlling bipolar cell responses to light stimulating photoreceptors to reverse spatial brightness contrast enhancement with the consequence that objects cannot be seen. This shows that the contrast of the retinal images is too low for vision without enhancement. However images consisting of randomly arranged small spots of different brightness are seen because of cones enhancing bipolar cell responses to rod input and rods enhancing bipolar cell responses to cone input. This residual vision and the observation that image distortion disappears at low light intensities reveal that macular degeneration is a functional disorder with intact photoreceptor function. The affection may therefore be caused by a reduction of blood flow through the choriocapillaries associated with ageing. The analysis of image distortion associated with the affection led to a simple way to determine the size of the affected retinal area, making it possible to follow the progression of the affection in a direct and simple way. Basic aspects are described of synaptic interaction within the information processing unit that determines the responses of the bipolar cells to photoreceptor input. This unit is the first information processing unit that has been revealed thanks to the extension of the analysis of the nervous system to the nanometer level at which information is processed. The minute size of the information processing unit, the special conditions for synaptic transmission combined with the short distances separating the synapses create special conditions for neural interaction at the nanometer level, establishing conditions for high speed neural communication.","PeriodicalId":17136,"journal":{"name":"Journal of submicroscopic cytology and pathology","volume":"36 3-4","pages":"295-303"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25294221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0