Evaluation of the in vitro effect of albendazole, metronidazole and nitazoxanide on viability and structure of Giardia lamblia cysts.

R Bernal-Redondo, L G Martínez-Méndez, A Mendoza-Chavez, D Velasco-Perales, B Chavez-Munguia
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Abstract

Effect of albendazole, metronidazole and nitazoxanide on the viability and structure of Giardia lamblia cysts isolates from infected children. The viability was evaluated by inducing excystation in a low-pH solution followed by an incubation in TYI-S culture medium. Nitazoxanide exhibited potent inhibitory effect (100%), metronidazole (79%) and albendazole (31%). The analysis among groups indicated a rs = 0.75 and p < 0.05. By TEM the cysts incubated with albendazole did not show morphological changes; with metronidazole, the formation of residual bodies in the nucleus border was observed. Incubated with nitazoxanide the damage to the cyst wall was evident, with the formation of areas with a granular content and the presence of cytoplasmic components in the peritrophic space. Our results propose that nitazoxanide showed a high effect on the viability and structure of G. lamblia cysts.

阿苯达唑、甲硝唑和硝唑尼特对兰氏贾第鞭毛虫囊体活力和结构影响的体外评价。
阿苯达唑、甲硝唑和硝唑肼对感染儿童蓝氏贾第鞭毛虫囊分离株活力和结构的影响。通过在低ph溶液中诱导细胞脱落,然后在TYI-S培养基中孵育来评估其生存能力。硝唑尼特(100%)、甲硝唑(79%)和阿苯达唑(31%)表现出较强的抑制作用。组间比较,r = 0.75, p < 0.05。用阿苯达唑孵育后,透射电镜观察未见形态学改变;使用甲硝唑时,观察到核边界残体的形成。用硝唑昔尼特孵育后,囊壁损伤明显,形成颗粒状区域,胞质成分出现在营养间隙。我们的结果表明,硝唑尼特对兰氏包囊的活力和结构有很高的影响。
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