Thais Silva Sales, V. C. A. Andrade Júnior, A. M. Azevedo, Marcelo Augusto Vieira Santos, Soryana Gonçalves Ferreira de Melo, M. C. Nery
{"title":"Tetrazolium test to assess the viability of kale seeds","authors":"Thais Silva Sales, V. C. A. Andrade Júnior, A. M. Azevedo, Marcelo Augusto Vieira Santos, Soryana Gonçalves Ferreira de Melo, M. C. Nery","doi":"10.1590/2317-1545v44261928","DOIUrl":"https://doi.org/10.1590/2317-1545v44261928","url":null,"abstract":"Abstract: Kale (Brassica oleracea var. acephala) has great importance due to its easy propagation, acceptability and nutraceutical properties. The aim of the present work was to make methodological adjustments to conduct the tetrazolium test in kale seeds. Pre-tests were initially carried out to assess the priming time at 20 °C for 10 and 14 hours (times defined by the imbibition curve) and methods of removing the coat of kale seeds (total removal of the seed coat; cut in the distal region to the embryonic axis; longitudinal cut along the longest axis and whole seeds). Subsequently, the most efficient methodologies were evaluated using different concentrations of the tetrazolium salt solution (0.075%; 0.2%; 0.5% and 1.0%) and times (2, 4 and 6 h) of seed immersion in the solution, using four lots. The seeds were analyzed individually and classified as viable or non-viable. The tetrazolium test is efficient for evaluating the viability of kale seeds, providing results correlated with germination. Kale seeds should be primed between paper for 10 hours at 20 °C, and the seed coat should be removed for immersion in a 0.5% tetrazolium salt solution for 4 hours at 30 °C.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tiago Darlan Grahl, A. P. Rockenbach, A. Casassola
{"title":"Outcomes of water deficit stress on the physiological quality of soybean seeds","authors":"Tiago Darlan Grahl, A. P. Rockenbach, A. Casassola","doi":"10.1590/2317-1545v44260716","DOIUrl":"https://doi.org/10.1590/2317-1545v44260716","url":null,"abstract":"Abstract: This study aimed to compare the physiological seed quality of soybean cultivars produced under or without water deficit stress. Seed samples of three soybean cultivars produced in the 2019/2020 crop season were collected in a rural property and in a company that obtains the cultivars. In the rural property, the seeds were produced under water deficit stress and high temperatures during the crop’s and were characterized by the presence of greenish seeds. In the company that obtains the cultivars, the sample consisted of a certified seed lot produced under normal rainfall and temperature conditions, without greenish seeds. Germination, average germination time, germination speed index, average germination speed, the number of normal and abnormal seedlings, the seedling height and dry weight, the root evaluations (length, surface area, volume, average diameter and dry mass) and the total seedling dry mass were also evaluated. Soybean seeds produced under water deficit stress showed a reduction in physiological quality directly proportional to the percentage of greenish seeds present in each seed lot. The occurrence of water deficit stress on the soybean in the reproductive and maturation stages negatively impacts the soybean crop installation in the subsequent season due to the low physiological seed quality.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
José Ricardo Bagateli, Ricardo Mari Bagateli, J. J. Franco, G. Meneghello, F. A. Villela
{"title":"Soybean sowing under low water availability conditions: impacts of seed treatment and soil management","authors":"José Ricardo Bagateli, Ricardo Mari Bagateli, J. J. Franco, G. Meneghello, F. A. Villela","doi":"10.1590/2317-1545v44256103","DOIUrl":"https://doi.org/10.1590/2317-1545v44256103","url":null,"abstract":"Abstract: The aim of this work was to evaluate the impact of soybean sowing in soil with low water availability, under two management systems, using seeds with and without chemical treatment, on the physiological quality of seeds and seedling emergence. For this, two experiments were set up, one under no-tillage system (NTS) and the other under conventional tillage system (CTS), in a randomized block design, both in a 2x6 factorial scheme, with two seed treatment conditions (with and without) and six periods of seed permanence in the soil (0, 24, 48, 72, 96 and 120 h), with four replications. Germination, vigor, percentage of abnormal seedlings and percentage of dead seeds were evaluated. Seed treatment made it possible to maintain germination at acceptable levels for up to five days of permanence of the seeds in the soil, even in CTS. Sowing in soils with low water availability can lead to reductions of 0.2 and 0.5 percentage points per hour of exposure to dry soil in germination and vigor of soybean seeds, respectively.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Pereira, F. Nery, Rafael Agostinho Ferreira, V. F. D. Silva, Mateus Moreira Bernardes, H. Santos, E. M. Bicalho
{"title":"Can priming with ascorbic acid or nitric oxide improve the germinability of stored sunflower seeds?","authors":"A. Pereira, F. Nery, Rafael Agostinho Ferreira, V. F. D. Silva, Mateus Moreira Bernardes, H. Santos, E. M. Bicalho","doi":"10.1590/2317-1545v44256600","DOIUrl":"https://doi.org/10.1590/2317-1545v44256600","url":null,"abstract":"Abstract: Sunflower (Helianthus annuus L.) is a relevant oilseed species used as feed in human and animal nutrition and in multiple industrial applications. However, oilseeds need to deal with the loss of vigor when used as a propagule, due to loss of seed physiological quality (viability and vigor) caused by deterioration events. We have evaluated in the present study the effects of seeds priming techniques (water, ascorbic acid, and sodium nitroprusside) on vigor, germination, antioxidant enzymes and membrane integrity of stored sunflower hybrid HELIO 251 seeds. Germination parameters showed that non-primed seeds (control) delayed germination in almost 72 h while all primed seeds germinated 100% in 24 h. Electrolyte leakage and malondialdehyde levels were higher in non-primed seeds indicating higher degree of membrane damage. An increase in the activity of catalase and ascorbate peroxidase was also observed after 8 h of imbibition of primed seeds compared to non-primed seeds. Regarding superoxide dismutase, there was no significant differences between treatments after 8 h of imbibition, whereas the highest activity was after 24 h of imbibition in non-primed seeds. Therefore, it can be concluded priming with ascorbic acid or sodium nitroprusside exhibited better performance in germination of stored sunflower seeds, probably resulting from lower accumulation of reactive oxygen species and consequent reduced oxidative damage due to an efficient antioxidant enzyme system.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. P. L. Cunha-Chiamolera, L. B. Lemos, A. P. Coelho, F. Mingotte
{"title":"Do the previous crop and top-dressing nitrogen fertilization change the yield and physiological and sanitary quality of common bean seeds?","authors":"T. P. L. Cunha-Chiamolera, L. B. Lemos, A. P. Coelho, F. Mingotte","doi":"10.1590/2317-1545v44257096","DOIUrl":"https://doi.org/10.1590/2317-1545v44257096","url":null,"abstract":"Abstract: The aim was to evaluate the effects of previous crop and top-dressing nitrogen fertilization on the yield and physiological and sanitary quality of common bean seeds. The design used was in randomized blocks in a split-plot scheme, with four replications. The plots were represented by three previous crops (sole maize, maize + Urochloa ruziziensis and sole U. ruziziensis), preceding common bean. The subplots consisted of N doses (urea) applied as top-dressing (0, 40, 80, 120 and 160 kg N.ha-1) when the common bean had four expanded leaves. Physiological quality and sanitary quality of the seeds were evaluated through germination tests, first count, emergence speed index, accelerated aging, seedling emergence in the field and pathogen incidence, in addition to crude protein content, 100-seed weight and yield. The study factors did not interfere in the sanitary quality of the seeds. Under U. ruziziensis straw, the highest seed yield was obtained with the N dose of 115 kg.ha-1, while under sole maize and intercropping straw, the increase in yield was linear. Previous crop with U. ruziziensis, whether cultivated as sole crop or intercropped with maize, promotes greater physiological quality of common bean seeds, while top-dressing nitrogen fertilization does not interfere with quality, weight and protein content of the seeds.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Everton Daniel Silva de Oliveira, T. Lima, Taise Almeida Conceição, R. M. Barbosa
{"title":"Ethanol test as a determinant of physiological potential of forest species seeds: The case of Plathymenia reticulata Benth. (Leguminosae-Mimosoideae), a tree of interest for Atlantic Forest restoration","authors":"Everton Daniel Silva de Oliveira, T. Lima, Taise Almeida Conceição, R. M. Barbosa","doi":"10.1590/2317-1545v44262795","DOIUrl":"https://doi.org/10.1590/2317-1545v44262795","url":null,"abstract":"Abstract: The ethanol test supplies information on physiological problems related to seed deterioration. The test optimization is crucial for its application in the seed analysis routine. This study aimed to evaluate vigor test for Plathymenia reticulataseeds based on ethanol production in order to establish execution guidelines and verify its relationship with other seed vigor tests. Seed lots were subjected to germination and vigor tests, and seed coat evaluation. Ethanol production assessment was carried out based on two trials testing water volumes for imbibition (0.25; 0.5; 0.75; 1.00; and 1.25 mL), reading periods (2, 4, 6, 8, 24, and 48 h), and seed quantities (5, 10, and 15). There was a distinction in physiological potential between the lots, indicated by the ethanol test and other traditionally used tests. The ethanol test was efficient for vigor assessment in Plathymenia seeds, as were the other vigor tests, when conducted with 15 seeds imbibed in a volume of water 2.5 times their mass for 48 h. The vigor test based on ethanol production proved reliable and it is recommended for use in a system of seed production and commercialization in nurseries and forestry companies.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Elaine Cristina Batista, Gabriel Mendes Villela, R. Pires, H. Santos, E. R. Carvalho, A. T. Bruzi
{"title":"Physiological quality of soybean seeds and the influence of maturity group","authors":"Elaine Cristina Batista, Gabriel Mendes Villela, R. Pires, H. Santos, E. R. Carvalho, A. T. Bruzi","doi":"10.1590/2317-1545v44261325","DOIUrl":"https://doi.org/10.1590/2317-1545v44261325","url":null,"abstract":"Abstract: Productive soybean genotypes is one of the main objectives of soybean breeding programs. However, there are few studies on the selection of characteristics associated with the quality and performance of soybean seeds. The objective in this study was to predict physiological and genetic parameters associated with seed quality in segregating soybean populations derived from parents of differing maturity groups. Eight genotypes of the F2:3 segregating soybean population (numbered 163, 24, 57, 108, 164, 169, 157, and 81) cultivated in the 2018/19 harvest were evaluated. To conduct the experiment, a completely randomized design was used. The physiological quality of the seeds was evaluated by germination test, tetrazolium test, and image analysis. Statistical analyses were performed by using a mixed model approach. Greater seed vigor was observed in the genotypes 108, 164, and 169, whose results were among the best for most of the analyzed parameters. The greater mechanical damage and damage due to stink bugs can explain the lower vigor of some seeds, as observed in genotypes 57 and 163, while weathering damage did not correspond to the results of the seed vigor tests. The analyses indicated a greater influence of genetic characteristics than of relative maturity group on the responses of the genotypes. No effect of relative maturity group on soybean seed vigor was evident.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Giselle Márcia de Melo, H. Santos, Thaísa Fernanda Oliveira, Antônio Rodrigues da Cunha Neto, A. Pereira, Marilia Mendes dos Santos Guaraldo
{"title":"Effect of priming and different types of drying on the physiological quality of Urochloa ruziziensis seeds","authors":"Giselle Márcia de Melo, H. Santos, Thaísa Fernanda Oliveira, Antônio Rodrigues da Cunha Neto, A. Pereira, Marilia Mendes dos Santos Guaraldo","doi":"10.1590/2317-1545v43250046","DOIUrl":"https://doi.org/10.1590/2317-1545v43250046","url":null,"abstract":"Abstract: Seed vigor is one of the main limitations to meet the demanding forage market. In order to boost the productive potential and provide the satisfactory development of quality seeds and seedlings, it is necessary to develop techniques that provide quality and high productivity. With the Urochloa genus stands out in the seed production and quality, this study aimed to evaluate the effect of seed priming with sodium nitroprusside and to define the best drying method that allows the maintenance of the changes of this treatment in Urochloa ruziziensis seeds. The seeds were conditioned in sodium nitroprusside solution, as a nitric oxide donor, for 42 hours and later submitted to the following procedures: no drying, initial reduction of water content, thermal shock, slow and fast drying. The design was completely randomized, with ten treatments and four replications. Water content, germination, emergence and biochemical evaluations were performed. As results, it was observed that all drying methods evaluated reduced the water content of the seeds to 12%, recommended range for storage, with the exception of the treatment without drying that presented 30%. Regarding germination parameters, the treatment of initial reduction and quick drying obtained the best performance in germination and emergence. Moreover, the types of drying presented high rate of enzymatic activity of the antioxidant system, thus helping in the maintenance of redox homeostasis. According to the enzymatic activity and germination rates, it suggests that the rapid drying method after priming with nitric oxide is the best method for Urochloa ruziziensis.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41537509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Respiratory rates and freezing tolerance of embryos of Inga vera Willd. subsp. affinis (DC.) T.D. Penn. from different regions","authors":"E. V. Lamarca, C. J. Barbedo","doi":"10.1590/2317-1545v43247247","DOIUrl":"https://doi.org/10.1590/2317-1545v43247247","url":null,"abstract":"ABSTRACT: Recalcitrant seeds are sensitive to desiccation and low storage temperatures, but there is a gradient of recalcitrance between different species and between populations of the same species. Therefore, tolerance to drying and low temperatures, as well as the respiratory rates of seeds, can be conditioned by the source of the material. The present study aimed to evaluate the relationship among desiccation and low temperature tolerance, and respiratory rates of Inga vera embryos from different regions. The embryos were submitted to three drying levels and incubated for up to fifteen days at -4, -2, 2 and 5 °C. Then, they were evaluated for respiration, germination and electrical conductivity. The embryos tolerated freezing at -4 °C since they were associated with certain levels of drying and a consequent change in the energy status of water. In conclusion, the conditions in which the embryos are formed define their degree of maturation at dispersal, hence their tolerance to desiccation and freezing.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43759035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Physiological performance of Coccoloba gigantifolia seeds subjected to desiccation and storage","authors":"S. A. D. N. Ferreira, D. Gentil, C. Ferreira","doi":"10.1590/2317-1545v43250757","DOIUrl":"https://doi.org/10.1590/2317-1545v43250757","url":null,"abstract":"Abstract: Coccoloba gigantifolia is recently described species, with an area of restricted occurrence and threatened with extinction, whose great ornamental potential is due to the size of its leaves and the architecture of the plant. The objective of this study was to evaluate the viability of its seeds under different water contents and storage temperatures, through two experiments. The first one used a completely randomized design (CRD) with six treatments (drying periods: 0, 1, 2, 3, 4 and 5 days) and four replications. The second experiment used a CRD in a 2 (storage temperatures: 8.0 ± 1.2 and 19.5 ± 0.7 °C) x 3 (storage periods: 1, 2 and 4 months) factorial scheme, in addition to a control treatment (without storage), with four replications. The seeds have a recalcitrant behavior, as they are sensitive to desiccation, with a marked reduction in viability with moisture content below 19.5%. Storage at temperatures of 8.0 and 19.5 °C did not maintain the physiological quality of the seeds, with 25.2% water, and the largest losses were found at a temperature of 8.0 oC.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2021-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45579210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}