Marie Grulichová, Veronika Sedláková, V. Trojan, P. Hanáček, T. Vyhnánek
{"title":"Correlation of photosynthetic pigments content with indicators of seed quality in the seeds of carrot, celery, dill, parsley, and parsnip","authors":"Marie Grulichová, Veronika Sedláková, V. Trojan, P. Hanáček, T. Vyhnánek","doi":"10.1590/2317-1545v44260767","DOIUrl":"https://doi.org/10.1590/2317-1545v44260767","url":null,"abstract":"Abstract: The content of photosynthetic pigments, especially chlorophylls, has a significant effect on the quality, viability, and storability of seeds. Determination of photosynthetic pigments together with the correlation with seed quality parameters, such as germination and radicle emergence, lead to the possibility of using the pigments content as a new indicator of seed quality. The photosynthetic pigments content was determined spectrophotometrically from extracts of commercial mature seeds of carrot, celery, dill, parsley, and parsnip. The content of chlorophyll a, chlorophyll b, β-carotene, and lutein varied among species of family Apiaceae and among varieties within species. Spectrophotometry was verified as easy, quick, and inexpensive method that can be used for the determination of photosynthetic pigments in mature seeds. The individual pigments content was compared to seed quality parameters such as standard germination, germination speed index (GSI), and radicle emergence (RE), which was carried out at 72, 96, 120, 144, and 168 h. Based on the correlation of photosynthetic pigments content with seed quality parameters (GSI and RE), chlorophyll b and lutein content were selected, and can be used as the new markers of seed quality.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Scarification and doses of Acadian®, Stimulate® and Trichoderma spp. promote dormancy overcoming in Hymenaea courbaril L. seeds?","authors":"O. J. Smiderle, A. D. G. Souza","doi":"10.1590/2317-1545v44250043","DOIUrl":"https://doi.org/10.1590/2317-1545v44250043","url":null,"abstract":"Abstract: The present study establishes the following research problem: Can different concentrations of Acadian®, Stimulate®, and Trichoderma spp. be effective in promoting the emergence and vigor of Hymenaea courbaril seedlings with or without mechanical scarification? The experimental design used was completely randomized, in a 4 x 3 x 2 factorial scheme, corresponding to four concentrations (0, 5.0, 10.0, and 15.0 mL.L-1), three hormones (Acadian®, Stimulate® and Trichoderma spp.), with and without mechanical scarification, with four replicates, each of which consisting of 25 seeds. Acadian® at a concentration of 5.0 mL.L-1 in scarified seeds of H. courbaril leads to a greater increase in the percentage of emergence and percentage of normal seedlings. Stimulate® at concentrations of 10.0 and 15.0 mL.L-1 is efficient in obtaining more than 85% emergence seedlings with seeds subjected to mechanical scarification. Trichoderma spp. from 9.0 mL.L-1 does not favor gains in the percentage of normal seedlings of Hymenaea courbaril grown from seeds with and without mechanical scarification. The dose of maximum technical efficiency (DMTE) of the bioregulator Acadian® is 8.50 mL.L-1 and, in scarified seeds of H. courbaril, it increases the percentage of seedling emergence.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. S. Martins, Nelson Augustos dos Santos Junior, C. J. Barbedo
{"title":"Seed pathology of non-domesticated species of tropical ecosystems","authors":"R. S. Martins, Nelson Augustos dos Santos Junior, C. J. Barbedo","doi":"10.1590/2317-1545v44262592","DOIUrl":"https://doi.org/10.1590/2317-1545v44262592","url":null,"abstract":"Abstract: Seed pathology is an area of study that began in the last century and has been developing since then, with increasing contribution especially to agricultural production systems. However, in the environmental area, studies began much later, but showed equal importance, especially for plant restoration and germplasm conservation programs. In this review, information about the knowledge on the pathology of seeds of non-domesticated species from tropical ecosystems is presented and the benefits and gaps of these studies are discussed.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The efficiency of prechilling and gibberellic acid (GA3) for breaking thermodormancy in lettuce","authors":"Gamze Kaya","doi":"10.1590/2317-1545v44262833","DOIUrl":"https://doi.org/10.1590/2317-1545v44262833","url":null,"abstract":"Abstract: The study focused on increasing the germination of lettuce cultivars under high-temperature conditions by seed treatments. The seeds of lettuce cultivars Yedikule, Iceberg, Iri Kıvırcık, and BT Iri Kıvırcık with different leaf morphology were primed by different doses of gibberellic acid (GA3) (0, 250, 500, and 1000 ppm) and preconditioned by chilling and heating. The seeds were germinated at optimal (20 °C) and high (35 °C) temperatures. The results showed that high temperature adversely influenced the germination percentage of lettuce cultivars. The mean germination percentage was 16.8% at 35 °C and 93.4% at 20 °C. Also, there was a significant difference among lettuce cultivars for germination at 35 °C. Iri Kıvırcık and BT Iri Kıvırcık showed a severe thermodormancy and hardly ever germinated at 35 °C. Prechilling and preheating promoted germination at 35 °C compared to unprimed seeds, but prechilling resulted in higher germination than preheating. GA3 was found to be the most useful treatment for improving germination compared to the others. The germination percentage of Iceberg, Yedikule, and Iri Kıvırcık primed with GA3 doses reached over 80%. The results suggested that the germination performance of lettuce cultivars suffering from thermodormancy or wherever climatic conditions with high temperatures should be promoted by the application of 500 ppm de GA3.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. T. Pinheiro, D. Dias, José Maria da Silva, Guilherme Fontes Valory Gama, M. J. Z. León, L. J. Silva
{"title":"Assessment of the tolerance of soybean seeds to weathering deterioration in the pre-harvest phase by multivariate analysis","authors":"D. T. Pinheiro, D. Dias, José Maria da Silva, Guilherme Fontes Valory Gama, M. J. Z. León, L. J. Silva","doi":"10.1590/2317-1545v44263001","DOIUrl":"https://doi.org/10.1590/2317-1545v44263001","url":null,"abstract":"Abstract: Weathering deterioration in the pre-harvest phase impairs high-quality soybean seed production. The performance of several tests to infer seed quality is common in genotype selection, and multivariate statistics can assist in the interpretations. This study aimed to assess the efficiency of the principal component analysis (PCA) and canonical discriminant analysis (CDA) multivariate statistical methods in assessing the tolerance of seeds of different soybean cultivars to weathering deterioration in the pre-harvest phase under greenhouse conditions. Seeds of six soybean cultivars (DM 6563, BMX Apolo, BMX Potência, NA 5909, NS 5959, and TMG 1175) were produced. Different simulated precipitation levels (0, 60, 120, and 180 mm) were applied in the pre-harvest phase. The seeds were collected and assessed for physiological, physical, and biochemical analyses and the data were analyzed by PCA and CDA techniques. The results showed that PCA and CDA are efficient for assessing the tolerance to weathering deterioration in soybean seeds. PCA and CDA assisted in the recommendation of the tests first germination count, accelerated aging, tetrazolium, percentage of seeds with seed coat wrinkling, protein content, and protease activity in the pre-selection of genotypes for weathering deterioration. PCA and CDA also helped to identify the cultivars DM 6563 and BMX Potência as more susceptible and NA 5909 and TMG 1175 as more tolerant to weathering deterioration in the pre-harvest phase.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Wheat seed germination based on α-amylase activity to study promoting mechanism of Bacillus subtilis QM3","authors":"Q. Hu, Jingnan Guo, J. Liu","doi":"10.1590/2317-1545v44263126","DOIUrl":"https://doi.org/10.1590/2317-1545v44263126","url":null,"abstract":"Abstract: The mechanism of promoting wheat seed germination by Bacillus subtilis has been paid great attention by many scholars. The germination rate and α-amylase activity of wheat seeds were significantly increased after germinating with Bacillus subtilis QM3 in this paper. Inhibitor and promoter of α-amylase were used to study relationship between bacteria and α-amylase. Compared with inhibitor group (10 mmol.L-1 EDTA), α-amylase activity of seeds treated by B. subtilis QM3 (106 CFU.mL-1) increased by 19.8%. It indicates that the inhibition has been alleviated. Similarly, α-amylase activity of co-treated group (2 mmol.L-1 CaCl2 and 106 CFU.mL-1 B. subtilis QM3) was higher than that of the promoter alone, reaching 14.9%. Furthermore, the results of α-amylase isozyme electrophoresis showed that there were three isozyme types in the gels, and the expression of α-amylase isoenzyme was significantly increased after treatment with B. subtilis QM3 (106 CFUmL.-1), which was reflected in the width and brightness of band mainly, especially band Cα. In addition, germination rate, α-amylase activity and isozyme electrophoresis of other three kinds of wheat seeds were also tested and similar results were obtained. Therefore, one of the possible mechanisms by which B. subtilis QM3 promotes seed germination is as a potential exogenous factor that can enhance activity and expression of α-amylase.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soryana Gonçalves Ferreira de Melo, V. C. A. Andrade Júnior, R. Pires, Dayliane Bernardes de Andrade, R. A. Santana, M. C. Nery
{"title":"Dormancy and evaluation of the physical-physiological quality in sweet potato [Ipomoea batatas (L.) Lam.] seeds by image analysis","authors":"Soryana Gonçalves Ferreira de Melo, V. C. A. Andrade Júnior, R. Pires, Dayliane Bernardes de Andrade, R. A. Santana, M. C. Nery","doi":"10.1590/2317-1545v44261490","DOIUrl":"https://doi.org/10.1590/2317-1545v44261490","url":null,"abstract":"Abstract: For the germination of sweet potato (Ipomoea batatas) seeds to occur, it is necessary to overcome dormancy, which makes it difficult to assess their physiological potential. The aim of this research was to define a methodology to overcome the dormancy of sweet potato seeds and use image analysis to determine their physical and physiological quality. Four genotypes of sweet potato seeds were used, namely UFVJM-5, UFVJM-22, UFVJM-38 and UFVJM-65. To overcome dormancy, chemical scarification with 98% H2SO4 for 10 and 20 minutes, hot water at 95 °C for 10 and 20 minutes, and mechanical scarification with electric grinder were tested. To evaluate the physical quality, the seeds were analyzed with X-rays and classified as intact, empty and malformed seeds. Using the GroundEye® system, the characteristics of color dominance, seed geometry and seedling length were quantified. Treatments with mechanical scarification with grinder and 98% sulfuric acid, for an immersion period of 20 minutes, are efficient to break dormancy in sweet potato seeds. There are genetic divergences between sweet potato genotypes, promoting the formation of different groups. The characteristics of color and geometry of the seeds are the ones that most contribute to genetic diversity of genotypes.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. C. Belniaki, M. D. C. Molinari, F. A. Henning, Maristela Panobianco
{"title":"Systematic Review - State of the Art: strategies for vigor evaluation and production of high-vigor soybean seeds","authors":"A. C. Belniaki, M. D. C. Molinari, F. A. Henning, Maristela Panobianco","doi":"10.1590/2317-1545v44268364","DOIUrl":"https://doi.org/10.1590/2317-1545v44268364","url":null,"abstract":"Abstract: A systematic approach was employed with the objective of compiling updated and more promising information on the quality of soybean seeds, traits, treatments and analysis techniques. Among the 6,899 academic materials retrieved between 2017 and 2022, 57 of them were included in the study, separated into three groups: Group 1 - Theoretical materials addressing traits related to seed vigor, which can be used in biotechnological strategies and improvement of different species; Group 2 - Practical materials that apply techniques of treatment of soybean seeds; and Group 3 - Practical materials that apply techniques to evaluate vigor in soybean seeds. From the approach used, it was possible to observe that several genes, proteins and QTLs are associated with seed vigor. Seed treatment techniques focus on the use of biological and physical products, but there is a lack of studies validating these benefits in the field. Among the methods for evaluating the vigor of soybean seeds, the techniques of image analysis (visible light, LIBS, NIR, FTIR, FT-NIR, HSI-NIR, FHSI, Hyperspectral, Fluorescence spectral) and the use of software (Vigor-S®, SVIS®, SAPL®, IJCropSeed, Ilastik, VideometerLab®, MATLAB, ENVI®) stand out.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. C. Bianchi, Nelson Júnior Dias Vilela, E. R. Carvalho, R. Pires, H. Santos, A. T. Bruzi
{"title":"Soybean seed size: how does it affect crop development and physiological seed quality?","authors":"M. C. Bianchi, Nelson Júnior Dias Vilela, E. R. Carvalho, R. Pires, H. Santos, A. T. Bruzi","doi":"10.1590/2317-1545v44255400","DOIUrl":"https://doi.org/10.1590/2317-1545v44255400","url":null,"abstract":"Abstract: The size of soybean seeds needs further study because its relationships with physiological quality, field establishment, and yield are controversial. The aim of this study was to evaluate the effects of seed size on soybean establishment and seed physiological quality. In the field, a 2x3x3 factorial design was used, with two cultivars (M 5947 IPRO and 59HO124 IPRO), three seed sizes (5.5, 6.0, and 6.5 mm), and three sowing densities (280,000, 320,000, and 360,000 plants.ha-1). Agronomic traits and grain yield were evaluated. After harvest, only seeds from plots with a population of 320,000 plants were classified by size using circular sieves with 5.5-mm, 6.0-mm, 6.5-mm, and 7.0-mm mesh sizes. For the evaluation of physiological quality, a 2x4 factorial design was applied, with two cultivars and four seed sizes. Germination, seedling emergence, accelerated aging, seedling dry matter, and emergence speed index were evaluated. Smaller seeds gave rise to shorter plants with a lower first pod insertion height. Larger seeds had higher physiological quality. The M 5947 IPRO cultivar showed better seed performance and quality.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fabiano França-Silva, S. M. Cicero, F. G. Gomes‐Junior, A. D. Medeiros, J. França-Neto, D. Dias
{"title":"Quantification of chlorophyll fluorescence in soybean seeds by multispectral images and their relationship with physiological potential","authors":"Fabiano França-Silva, S. M. Cicero, F. G. Gomes‐Junior, A. D. Medeiros, J. França-Neto, D. Dias","doi":"10.1590/2317-1545v44258703","DOIUrl":"https://doi.org/10.1590/2317-1545v44258703","url":null,"abstract":"Abstract: The multispectral image analysis technique to detect chlorophyll fluorescence (CF) in soybean seeds was studied to assess the relationship between CF signals and seed physiological potential. Eight treatments, corresponding to 0%, 2%, 4%, 6%, 8%, 10%, 12%, and 14% green seeds, were used on two cultivars, BMX Desafio RR 8473 RSF and 96R10 IPRO, which passed through different seed quality tests. Initially, the CF of the seeds was determined using 660 nm and 730 nm spectra, and then the germination, electrical conductivity, accelerated aging with saturated NaCl solution, tetrazolium, and computerized seedling image analysis (Vigor-S) tests were performed on the same seeds. A completely randomized design was used, as well as replications of each treatment. Analysis of variance (ANOVA) was performed on the data from germination, vigor, and CF tests using the R® software, and the means were grouped by the Scott-Knott test (p ≤ 0.05). Pearson’s linear correlation coefficients (r) were calculated for all combinations among the evaluations with significance of the r values determined by the t-test (p ≤ 0.05), and multivariate analysis of the principal components was performed. Proportional increases in green seeds contribute to an increase in chlorophyll fluorescence signals and have a negative correlation with seed physiological quality; levels above 4% green seeds in the samples result in marked losses in physiological potential. Therefore, the chlorophyll fluorescence detected through multispectral images is inversely related to the physiological potential of soybean seeds.","PeriodicalId":17113,"journal":{"name":"Journal of Seed Science","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67324175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}