Journal of Stem Cell Research and Tissue Engineering最新文献

{"title":"The effect of chitosan on phenolic compounds, rosmarinic acid and expression of key genes involved in rosmarinic acid biosynthesis in cell suspension culture of Melissa officinalis L.","authors":"A. Mahdavianfard, M. Dahajipour Heidarabadi, K. Malekzadeh, SR ahhafi","doi":"10.52547/jct.11.4.243","DOIUrl":"https://doi.org/10.52547/jct.11.4.243","url":null,"abstract":"","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84802471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Long Non-coding RNA CRNDE Expression in Iranian Patients with Colorectal cancer","authors":"S. Gholaminejad, Z. Deilami khiabani, A. Salehzadeh, A. Jalali","doi":"10.52547/jct.11.4.293","DOIUrl":"https://doi.org/10.52547/jct.11.4.293","url":null,"abstract":"","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77035553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect Of Mangrove Leaf Extract Dosage Rhizophora Mucronata Lmk. On The Viability Of Hela Cells","authors":"Rohadatul Alsy","doi":"10.20473/jscrte.v5i1.29382","DOIUrl":"https://doi.org/10.20473/jscrte.v5i1.29382","url":null,"abstract":"This study aims to determine the IC50 value of the mangrove leaf extract type Rhizophora mucronata Lmk. against the viability of Hela cells. The samples extracted with three types of solvents were previously conducted preliminary research on phytochemical compounds and their toxicity values with the Meted Brine Shrimp Letality Test (BSLT). In the toxicity test, the highest level of toxicity was obtained in the ethanol extract with a value of 166.72± 7.72 ppm, then the sample was continued for the cytotoxicity test using the MTT method. The dosage variants used in this study were 62.5 ppm; 125 ppm; 250 ppm; 500 ppm; and 1000 ppm. The variation in dosage shows an effect on the viability of Hela cells, namely a decrease in the percentage of living cells along with the addition of the ethanol extract dose of Rhizophora mucronata Lmk leaves. which is given. And the IC50 value obtained from this study was 63.67 µg / mL with the toxic category","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"279 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77577802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect Of Mangrove Leaf Extract Dosage Sonneratia Alba On Hela Cell Viability","authors":"Fitria Devi Suryaningrum","doi":"10.20473/jscrte.v5i1.29383","DOIUrl":"https://doi.org/10.20473/jscrte.v5i1.29383","url":null,"abstract":"Cervical cancer is caused due to infection from the Human Papilloma Virus (HPV) which attacks the sexually active female reproductive organs. Treatment is carried out alternatively using natural ingredients such as mangroves. Sonneratia alba is a type of mangrove plant that has been used in alternative medicine because of its potential as an anticancer. This study aims to determine the effect of Sonneratia alba mangrove extract on heLa cell viability. The Sonneratia alba leaf powder was extracted using stratified maceration. The solvents used include n-hexane, ethyl acetate, and ethanol. The results showed that the LC50 value was 3.59 ppm, this means that the ethyl acetate extract has toxic properties. Phytochemical test results of Sonneratia alba leaf extract contain alkaloid compounds, steroids / triterpenoids, and tannins. The results of the test yield extract were 12.60%, extract water content was 21.24%, and total phenol was 504.08 mg / g GAE Test The results of the LC-MS test resulted in the suspicion of compounds including diosmetin, caffeine, and turmeron. The ethyl acetate extract of Sonneratia alba leaves was cytotoxic against heLa cell viability with the resulting IC50 value of 478.630 µg / mL","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78976404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Dose Effect of Mangrove Leaf Extract (Rhizophora apiculata) on Anticancer Activity in HeLa Cells","authors":"Dwi Mahfud Maulana","doi":"10.20473/jscrte.v5i1.29380","DOIUrl":"https://doi.org/10.20473/jscrte.v5i1.29380","url":null,"abstract":"Disease cancer caused by abnormal growth of tissue where there has been an error, fast and out of control. Judging from the fact of gender, more than 270,000 women die every year caused by cervical cancer. To inhibit the growth of cancer cells, a compound is needed that causes the cell cycle to stop so that the ability of cell proliferation decreases. Alkaloid compounds can inhibit proliferation through oxidative inhibition processes that can cause cancer. Mangrove plants have potential as anticancer, antimicrobial, and antioxidant. The content of chemical compounds found in mangroves are flavonoids, steroids, alkaloids, phenolites, saponins and tannins. These compounds show high antioxidant activity and are shown to have a real relationship with the properties of the material's bioactivity against cancer cells. One of the mangrove species is Rhizophora apiculata. The purpose of this study was to determine the IC50 value produced by Rhizophora apiculata mangrove leaf extract on HeLa cell viability and to see the effect of Rhizophora apiculata mangrove leaf extract dosage on HeLa cell viability. The method used in this research is the experimental method. The research parameters included yield, proximate test, phytochemical test, toxicity test, total phenol test, cytotoxicity test and LC-MS test. The experimental design used was a simple and complex completely randomized design (CRD) with the Tukey test.The results of this study showed that the highest yield was in the ethanol extract of 5.91%, while the n-hexane and ethyl acetate extracts respectively had yields of 1.18% and 1.31%. The results of the proximate test on the water content of leaves and powder were 64.53% and 13.86%, respectively, the results of the ash content in the leaves and powder of Rhizophora apiculata were 3.94% and 8.41%, respectively. while the water content in the extract obtained the highest yield in the ethanol extract of 21.42%, while the n-hexane extract and ethyl acetate extract were 11.08% and 15.42%, respectively. For phytochemical results, it was found that n-hexane extract only contained alkaloids, flavonoids and steroids. Ethyl acetate extract contains steroid compounds. Meanwhile, the ethanol extract contains the most bioactive compounds, namely saponins, flavonoids, tannins and triterpenoids. The toxicity test using the Brine Shrimp Lethality Test (BSLT) method resulted in the lowest IC50 of ethanol extract at 49.45 ppm while the n-hexane and ethyl acetate extracts were 251.63 ppm and 920.45 ppm respectively. In the total phenol test, the n-hexane extract was 66.79 mg GAE / 100 gr, 222.97 mg GAE / 100 gr ethyl acetate extract and 929.04 mg GAE / 100 gr ethanol extract. HeLa cell cytotoxicity testing using the MTT method (3- (4,5-dimethiltiazol-2-yl) -2,5-dipheniltetra zolium bromide) assay resulted in the highest cell viability value at a dose of 125 ppm of 46.97%. As for the doses of 250 ppm, 500 ppm 1000 ppm, and 2000 ppm resulted in a percentage of viability","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75443587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect Of Alpha-Tocopherol On Spermatozoa Death Of Apoptosis and Necrosis in Rats (Rattus Norvegicus) Exposed 2,3,7,8- Tetrachlorodibenzo-P-Dioxin","authors":"Valderama Gomang","doi":"10.20473/jscrte.v5i1.29381","DOIUrl":"https://doi.org/10.20473/jscrte.v5i1.29381","url":null,"abstract":"The aims of this research was to investigate effect of alpha-tocopherol on spermatozoa death in form of apoptosis and necrosis in rats (Rattus norvegicus) exposed 2,3,7,8- tetrachlorodibenzo-p-dioxin. Male rats were administered TCDD and alpha-tocopherol in experimental groups. Five experimental groups of a combination of TCDD and alpha- tocopherol were designed as follows; 0.5 ml of corn oil (control negative group, K-), 700 ng/kg/d of TCDD and 0.5 ml of corn oil (treatment control), 700 ng/kg/d of TCDD and 77 ng/kg/d of alpha-tocopherol (Group P1), 700 ng/kg/d of TCDD and 140 mg/kg/d of alpha-tocopherol (Group P2), 700 ng/kg/d of TCDD and 259 mg/kg/d of alpha- tocopherol (Group P3) respectively. Alpha-tocopherol and TCDD were given by oral gavage for 20 days. The result indicated that TCDD increased spermatozoa death in form of apoptosis and also necrosis. Alpha-tocopherol at 259 mg/kg/d most effective to decreased spermatozoa death number. The conclusion indicated that alpha-tocopherol at 259 mg/kg/d effective to decreased the spermatozoa death in form of apoptosis and necrosis in rats (Rattus norvegicus) exposed 2,3,7,8-tetrachlorodibenzo-p-dioxin.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75986380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect Of Dosage Of Mangrove Leaf Extract Avicennia Marina On The Viability Of Hela Cells","authors":"Mahbubur Rahman","doi":"10.20473/jscrte.v5i1.29384","DOIUrl":"https://doi.org/10.20473/jscrte.v5i1.29384","url":null,"abstract":"Cervical cancer is caused by infection with the Human Papilloma Virus (HPV) which attacks the reproductive organs of sexually active women. Treatment is done alternatively using natural materials such as mangrove plants. Avicennia marina is a type of mangrove plant that has been used in alternative medicine because of its potential as an anticancer. This study aimed to determine the effect of Avicennia marina mangrove leaf extract on the viability of HeLa cells. Avicennia marina mangrove leaf powder was extracted using graded maceration. The solvents used include n-hexane, ethyl acetate, and ethanol. The results showed that the LC50 value was 98.55 ppm, it means that the ethanol extract has toxic properties. Phytochemical test results of Avicennia marina mangrove leaf extract contain saponins, steroids/triterpenoids, flavonoids and tannins. The test results showed that the extract yield was 14.40%, the water content of the extract was 16.57%, and the total phenol was 1915.92 mg/g GAE. The results of the LC- MS test resulted in suspected compounds including Caffeine and Diosmetin. The ethanol extract of Avicennia marina mangrove leaves was cytotoxic to heLa cell viability with the resulting IC50 value of 115.345 g/mL.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"65 6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89866423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Bone Marrow Transplantation on Oocyte-Granulosa Cell Interaction and Follicular Development of Cisplatin-Induced Ovarian Failure in Rat","authors":"H. Hendarto","doi":"10.20473/JSCRTE.V4I2.22751","DOIUrl":"https://doi.org/10.20473/JSCRTE.V4I2.22751","url":null,"abstract":"Introduction: Chemotherapy has cytotoxic effect that induces follicular damage and abnormal folliculogenesis leads to ovarian failure. Two crucial   growth factors in abnormal folliculogenesis, Growth Differentiation Factor-9 (GDF-9) and Kit-Ligand, will be disrupted and affect follicular development. In this study, we evaluate whether bone marrow transplantation (BMT) has a role on oocyte-granulosa cell interaction by analyzing GDF-9 and Kit-Ligand expressions and also follicular development by analyzing primordial, primary, secondary and graafian follicles of cisplatin-induced ovarian failure in rat. Material and method: Forty eight rats were divided into three groups: control, cisplatin and cisplatin + BMT. Ovarian failure was induced by administration   of intraperitoneal cisplatin 5 mg/kg body weight for 1 week. BMT 2 x107 cells were injected through rat tail vein after cisplatin administration.  Bone marrow was isolated from rat femur and characterized    by CD44(+), CD45(-), CD105(+). Immunohistochemistry examinations for ovarian GDF-9, Kit-Ligand and follicle development evaluation were performed after 2 weeks of BMT injection. Results:  The  expressions   of Kit-ligand  among  three  groups  by ANOVA were  significant different (p=0.00), whereas by Post Hoc: cisplatin group lower  than  control  group (p=0.00); cisplatin + BMT group  higher than  cisplatin group (p=0.00); and no significant different between  control  group and cisplatin + BMT group (p=0.955). The expressions of GDF-9 by Kruskal Wallis showed significant different (p=0.00) among three groups whereas cisplatin + BMT group higher than cisplatin group and control group. In cisplatin + BMT group the number of primordial, primary, secondary and graafian follicles were higher than those in cisplatin group; but were lower than those in control group (p=0.000). Positive Paul Kart Horan (PKH) labeling was seen in cisplatin + BMT   group only. Conclusion:  In cisplatin-induced ovarian failure in rat, bone marrow transplantation may improve oocytegranulosa cell interaction and follicular development.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89250693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of TiO2 nanoparticles on physiological and anatomical characteristics of Baby \u0000sun rose (Aptenia cordifolia)","authors":"L. Ahmadi, M. Kolahi, H. Mohajjel Shoja, E. MOHAJEL KAZEMI","doi":"10.52547/jct.11.3.203","DOIUrl":"https://doi.org/10.52547/jct.11.3.203","url":null,"abstract":"","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86059323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Correlation between NRF2Antioxidant Gene Expression and Sperm Quality in Asthenoteratozoospermia Men","authors":"R. Janati Far, L. Naser Pour, SS Sahraei, H. Piroozmanesh","doi":"10.52547/jct.11.3.178","DOIUrl":"https://doi.org/10.52547/jct.11.3.178","url":null,"abstract":"","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77263296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}