红树叶提取物对HeLa细胞抗癌活性的剂量效应

Dwi Mahfud Maulana
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One of the mangrove species is Rhizophora apiculata. The purpose of this study was to determine the IC50 value produced by Rhizophora apiculata mangrove leaf extract on HeLa cell viability and to see the effect of Rhizophora apiculata mangrove leaf extract dosage on HeLa cell viability. The method used in this research is the experimental method. The research parameters included yield, proximate test, phytochemical test, toxicity test, total phenol test, cytotoxicity test and LC-MS test. The experimental design used was a simple and complex completely randomized design (CRD) with the Tukey test.The results of this study showed that the highest yield was in the ethanol extract of 5.91%, while the n-hexane and ethyl acetate extracts respectively had yields of 1.18% and 1.31%. 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引用次数: 0

摘要

癌症疾病是由于组织生长异常的地方出现了错误,迅速而失控。从性别的事实来看,每年有超过27万的女性死于宫颈癌。为了抑制癌细胞的生长,需要一种能使细胞周期停止从而使细胞增殖能力下降的化合物。生物碱化合物可以通过氧化抑制过程来抑制增殖,而氧化抑制过程会导致癌症。红树林植物具有抗癌、抗菌和抗氧化的潜力。在红树林中发现的化合物有黄酮类化合物、类固醇、生物碱、酚类化合物、皂苷和单宁。这些化合物显示出很高的抗氧化活性,并被证明与材料抗癌细胞的生物活性有真正的关系。其中一个红树林物种是根茎草。本研究的目的是测定尖根藤红叶提取物对HeLa细胞活力的IC50值,并观察尖根藤红叶提取物用量对HeLa细胞活力的影响。本研究采用的方法是实验法。研究参数包括产率、近似试验、植物化学试验、毒性试验、总酚试验、细胞毒性试验和LC-MS试验。实验设计采用简单和复杂的完全随机设计(CRD),并采用Tukey检验。结果表明,乙醇提取物得率最高,为5.91%,正己烷和乙酸乙酯提取物得率分别为1.18%和1.31%。叶片和粉末含水量近似测定结果分别为64.53%和13.86%,叶片和粉末灰分含量近似测定结果分别为3.94%和8.41%。乙醇提取物的提取率最高,为21.42%,正己烷提取物和乙酸乙酯提取物的提取率分别为11.08%和15.42%。植物化学结果表明,正己烷提取物仅含生物碱、类黄酮和类固醇。乙酸乙酯提取物含有类固醇化合物。同时,乙醇提取物含有最具生物活性的化合物,即皂苷、黄酮类、单宁和三萜。采用卤虾致死试验(BSLT)法进行毒性试验,乙醇提取物的IC50最低,为49.45 ppm,正己烷和乙酸乙酯提取物的IC50最低,分别为251.63 ppm和920.45 ppm。总酚试验中,正己烷提取物为66.79 mg GAE / 100 gr,乙酸乙酯提取物为222.97 mg GAE / 100 gr,乙醇提取物为929.04 mg GAE / 100 gr。采用MTT法(3-(4,5-二甲基噻唑-2-基)-2,5-二苯四溴唑)测定HeLa细胞毒性,在125 ppm(46.97%)剂量下细胞活力值最高。250 ppm、500 ppm、1000 ppm和2000 ppm的活菌率分别为42.95%、37.70%、35.82%和32.12%。细根参叶提取物的IC50值为64.42 ppm。该值表明,根茎提取物对HeLa细胞有毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Dose Effect of Mangrove Leaf Extract (Rhizophora apiculata) on Anticancer Activity in HeLa Cells
Disease cancer caused by abnormal growth of tissue where there has been an error, fast and out of control. Judging from the fact of gender, more than 270,000 women die every year caused by cervical cancer. To inhibit the growth of cancer cells, a compound is needed that causes the cell cycle to stop so that the ability of cell proliferation decreases. Alkaloid compounds can inhibit proliferation through oxidative inhibition processes that can cause cancer. Mangrove plants have potential as anticancer, antimicrobial, and antioxidant. The content of chemical compounds found in mangroves are flavonoids, steroids, alkaloids, phenolites, saponins and tannins. These compounds show high antioxidant activity and are shown to have a real relationship with the properties of the material's bioactivity against cancer cells. One of the mangrove species is Rhizophora apiculata. The purpose of this study was to determine the IC50 value produced by Rhizophora apiculata mangrove leaf extract on HeLa cell viability and to see the effect of Rhizophora apiculata mangrove leaf extract dosage on HeLa cell viability. The method used in this research is the experimental method. The research parameters included yield, proximate test, phytochemical test, toxicity test, total phenol test, cytotoxicity test and LC-MS test. The experimental design used was a simple and complex completely randomized design (CRD) with the Tukey test.The results of this study showed that the highest yield was in the ethanol extract of 5.91%, while the n-hexane and ethyl acetate extracts respectively had yields of 1.18% and 1.31%. The results of the proximate test on the water content of leaves and powder were 64.53% and 13.86%, respectively, the results of the ash content in the leaves and powder of Rhizophora apiculata were 3.94% and 8.41%, respectively. while the water content in the extract obtained the highest yield in the ethanol extract of 21.42%, while the n-hexane extract and ethyl acetate extract were 11.08% and 15.42%, respectively. For phytochemical results, it was found that n-hexane extract only contained alkaloids, flavonoids and steroids. Ethyl acetate extract contains steroid compounds. Meanwhile, the ethanol extract contains the most bioactive compounds, namely saponins, flavonoids, tannins and triterpenoids. The toxicity test using the Brine Shrimp Lethality Test (BSLT) method resulted in the lowest IC50 of ethanol extract at 49.45 ppm while the n-hexane and ethyl acetate extracts were 251.63 ppm and 920.45 ppm respectively. In the total phenol test, the n-hexane extract was 66.79 mg GAE / 100 gr, 222.97 mg GAE / 100 gr ethyl acetate extract and 929.04 mg GAE / 100 gr ethanol extract. HeLa cell cytotoxicity testing using the MTT method (3- (4,5-dimethiltiazol-2-yl) -2,5-dipheniltetra zolium bromide) assay resulted in the highest cell viability value at a dose of 125 ppm of 46.97%. As for the doses of 250 ppm, 500 ppm 1000 ppm, and 2000 ppm resulted in a percentage of viability of 42.95% 37.70% 35.82% and 32.12%, respectively. The IC50 value of Rhizophora apiculata leaf extract was 64.42 ppm. This value indicates that the Rhizophora apiculata extract is toxic to HeLa cells.
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