Journal of Stem Cell Research and Tissue Engineering最新文献

CHONDROREGENERATIVE POTENTIAL ON SUBCUTANEOUS IMPLANTATION OF PLATELET-RICH FIBRIN (PRF)-IMPREGNATED DECELLULARIZED BOVINE CARTILAGE SCAFFOLD 皮下植入富血小板纤维蛋白（prf）浸渍脱细胞牛软骨支架的软骨再生潜力

Journal of Stem Cell Research and Tissue Engineering Pub Date : 2023-12-12 DOI: 10.20473/jscrte.v7i2.52555

P. Widyatmika, M. S. Noer, Magda Rosalina Hutagalung

{"title":"CHONDROREGENERATIVE POTENTIAL ON SUBCUTANEOUS IMPLANTATION OF PLATELET-RICH FIBRIN (PRF)-IMPREGNATED DECELLULARIZED BOVINE CARTILAGE SCAFFOLD","authors":"P. Widyatmika, M. S. Noer, Magda Rosalina Hutagalung","doi":"10.20473/jscrte.v7i2.52555","DOIUrl":"https://doi.org/10.20473/jscrte.v7i2.52555","url":null,"abstract":"The discovery of alternative implants with regenerative potential comparable to autologous cartilage continues to be encouraged because of the high donor site morbidity rate. This research tries to make an alternative implant that uses the concept of tissue engineering techniques in the form of endogenous regeneration by combining Decellularized Bovine Cartilage scaffold with Platelet-Rich Fibrin (BCPRF) which is implanted subcutaneously. The aim of this study was to compare the potential for chondroregeneration between BCPRF and autologous cartilage as assessed by chondrocyte cell formation, type 2 collagen thickness, and implant resorption rate in subcutaneous implantation. Using the research design method is a pretest-posttest control group design using New Zealand white rabbits. Forty eight experimental samples were divided into 2 groups which were treated with BCPRF and autologous cartilage implantation. Results were evaluated after 6 weeks. Evaluation was carried out on 39 samples. Microscopy showed better potential for autologous cartilage chondroregeneration than BCPRF with significant differences in the number of chondrocytes formed, the thickness of type 2 collagen (p=0.000), and the rate of implant resorption (p=0.000). In conclusion, the potential for chondroregeneration of autologous cartilage and BCPRF is significantly different in terms of the number of chondrocytes formed, the thickness of type 2 collagen, and the rate of implant resorption.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139010005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE EFFECT OF PLATELET-RICH PLASMA ADMINISTRATION ON PROLIFERATION AND DIFFERENTIATION OF ENDOTHELIAL PROGENITOR CELLS IN PATIENTS WITH STABLE CORONARY HEART DISEASE 服用富血小板血浆对稳定型冠心病患者内皮祖细胞增殖和分化的影响

Journal of Stem Cell Research and Tissue Engineering Pub Date : 2023-12-12 DOI: 10.20473/jscrte.v7i2.52561

Ronald Rendy Hehanusa

{"title":"THE EFFECT OF PLATELET-RICH PLASMA ADMINISTRATION ON PROLIFERATION AND DIFFERENTIATION OF ENDOTHELIAL PROGENITOR CELLS IN PATIENTS WITH STABLE CORONARY HEART DISEASE","authors":"Ronald Rendy Hehanusa","doi":"10.20473/jscrte.v7i2.52561","DOIUrl":"https://doi.org/10.20473/jscrte.v7i2.52561","url":null,"abstract":"Endothelial Progenitor Cells (EPCs) are crucial precursors to endothelial cells, playing a key role in regulating blood vessel structure and maintaining homeostasis to protect against inflammation and thrombosis, contributing to stable coronary heart disease (CHD). Growth factors stimulate signal transduction during EPC proliferation and differentiation. Platelet-Rich Plasma (PRP) contains widely recognized growth factors in angiogenesis. Our research aimed to analyze PRP's effects on EPC proliferation and differentiation in stable CHD patients. Using an experimental post-test control group design, mononuclear cells (MNCs) from peripheral blood were cultured with M-199 medium, divided into PRP, Platelet Poor Plasma (PPP), and control groups, and incubated for 14 days. EPC proliferation was quantified with CD34 markers using ANOVA. After 7 days, differentiated cells were counted with von Willebrand Factor (vWF) markers using the Mann-Whitney U test.EPC proliferation significantly increased in the PRP group (1.052 ± 0.16) compared to PPP (0.762 ± 0.19) and the control (0.068 ± 0.05, p=0.000). However, EPC differentiation showed no significant increase in the PRP group compared to PPP (0.00-0.30 vs. 0.00-0.20, p = 0.565) or the control (0.00-0.30 vs. 0.00-0.00, p = 0.064). Additionally, no significant increase in EPC differentiation was observed in the PPP group compared to the control (0.00-0.20 vs. 0.00-0.00, p = 0.144). PRP significantly enhanced EPC proliferation but did not significantly enhance differentiation in the peripheral blood of stable CHD patients compared to PPP and control groups.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139009098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PROFILE OF NUTRITIONAL STATUS, HEMOGLOBIN, LEUKOCYTES, PLATELET, HEMATOCRIT, ALBUMIN AND SEROLOGY TEST IN CHILDREN INFECTED WITH DENGUE VIRUS AT DR SOETOMO HOSPITAL YEAR 2015 2015年DR SOETOMO医院感染登革热病毒儿童的营养状况、血红蛋白、白细胞、血小板、血细胞比容、白蛋白和血清学检验概况

Journal of Stem Cell Research and Tissue Engineering Pub Date : 2023-12-12 DOI: 10.20473/jscrte.v7i2.52558

Zakirah Zuhra

{"title":"PROFILE OF NUTRITIONAL STATUS, HEMOGLOBIN, LEUKOCYTES, PLATELET, HEMATOCRIT, ALBUMIN AND SEROLOGY TEST IN CHILDREN INFECTED WITH DENGUE VIRUS AT DR SOETOMO HOSPITAL YEAR 2015","authors":"Zakirah Zuhra","doi":"10.20473/jscrte.v7i2.52558","DOIUrl":"https://doi.org/10.20473/jscrte.v7i2.52558","url":null,"abstract":"The Dengue Virus Infection (DVI) triggers changes in the host's hematological, biochemical, and immunological aspects, affecting variables like hemoglobin (Hb), leukocytes (WBC), platelets (Plt), hematocrit (Hct), albumin (Alb), IgM, IgG, and NS1Ag Dengue. This study aims to investigate the demographic and clinical characteristics of DVI patients, considering factors such as gender, age, nutritional status, Hb, WBC, Plt, Hct, Alb, dengue serologic tests, and DVI stage. Using a cross-sectional descriptive approach, medical records of 74 subjects were analyzed. Subjects were categorized as DF (33.78%), DHF I (27.03%), DHF II (4.05%), DHF III (25.68%), and DHF IV (9.46%). Predominantly, subjects were aged 6-12 years (48.65%) and predominantly male (58.11%). Most patients had a normal nutritional status (50%), seen in both females and males (32.26%, 62.79%), with DF being dominant (14.86%, 28.92%). Over-nutrition occurred in DF, DHF without shock, and DHF with shock at rates of 12.5%, 33.33%, and 54.17%. DF prevailed in patients aged <6 years (17.57%), while DHF I, DHF II, DHF III were more common in those aged 6-12 years (14.86%, 2.70%, 16.22%), and DHF IV in those aged <6 years (8.11%). Key findings revealed increased Hb levels from DF to DHF, with 38 of 74 DVI patients exhibiting leukopenia. The DVI stage showed an inverse correlation with Plt levels. Hct levels rose in DHF patients, and they had relatively low Alb levels. Primary infections were more frequent in DF, while secondary infections were predominant in DHF with shock. The study also noted variations in over-nutrition prevalence across DVI stages among patients at Dr. Soetomo General Hospital Surabaya in 2015.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139008254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CYTOTOXICITY TEST OF BOVINE DEMINERALIZED BONE MATRIX ON HUMAN MESENCHYMAL STEM CELLS USING THE MTT ASSAY METHOD 用 Mtt 检测法测试牛脱矿骨基质对人间质干细胞的细胞毒性

Journal of Stem Cell Research and Tissue Engineering Pub Date : 2023-12-12 DOI: 10.20473/jscrte.v7i2.52559

Paulus Ronald Hibono

{"title":"CYTOTOXICITY TEST OF BOVINE DEMINERALIZED BONE MATRIX ON HUMAN MESENCHYMAL STEM CELLS USING THE MTT ASSAY METHOD","authors":"Paulus Ronald Hibono","doi":"10.20473/jscrte.v7i2.52559","DOIUrl":"https://doi.org/10.20473/jscrte.v7i2.52559","url":null,"abstract":"The use of bone grafts in Indonesia continues to increase each year. Although Autograft is considered the gold standard in bone grafting, its use is often confronted with various challenges, similar to allograft. To address this issue, Bovine Demineralized Bone Matrix (DBM) can be considered as a substitute for bone grafts with the advantages of unlimited availability and more affordable costs. Currently, the Tissue Bank of Dr. Soetomo Hospital is developing bovine DBM, although there is no research yet on its potential toxicity.This study aims to evaluate whether bovine DBM has cytotoxic effects on human mesenchymal stem cells. In this experimental study, a total of 48 samples were involved, including a control group and two treatment groups (50% and 25%), each consisting of 16 samples. Mesenchymal stem cells were cultured and then treated with the addition of 50% and 25% DBM. Subsequently, cell viability was measured using the MTT Assay method.The collected data were processed by conducting normality and homogeneity tests and then analyzed using comparative tests with an independent t-test. The criteria for declaring cell toxicity were set at a viability of not less than 60% compared to the control group.The results of the MTT assay measurements showed that the mean Optical Density (OD) in the control group was 0.656 ± 0.021 (range 0.620-0.696), while in the treatment groups, it was 0.565 ± 0.022 (range 0.529-0.614) and 0.520 ± 0.022 (range 0.461-0.552), respectively. Statistically, the differences in OD between the control group and both treatment groups (50% and 25%) were significant (p<0.05). The average cell viability in both treatment groups was found to be more than 60%, indicating that Bovine Demineralized Bone Matrix is not toxic to human mesenchymal stem cells.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139008444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

INCREASE MIGRATION OF PERIPHERAL BLOOD DERIVED ENDOTHELIAL PROGENITOR CELLS OF STABLE CORONARY ARTERY DISEASE PATIENT WITH ANGIOTENSIN CONVERTING ENZYME INHIBITORS 使用血管紧张素转换酶抑制剂增加稳定型冠心病患者外周血内皮祖细胞的迁移率

Journal of Stem Cell Research and Tissue Engineering Pub Date : 2023-12-12 DOI: 10.20473/jscrte.v7i2.52557

Hanang Anugrawan Achmad, Yudi Her Oktaviano, Djoko Soemantri

{"title":"INCREASE MIGRATION OF PERIPHERAL BLOOD DERIVED ENDOTHELIAL PROGENITOR CELLS OF STABLE CORONARY ARTERY DISEASE PATIENT WITH ANGIOTENSIN CONVERTING ENZYME INHIBITORS","authors":"Hanang Anugrawan Achmad, Yudi Her Oktaviano, Djoko Soemantri","doi":"10.20473/jscrte.v7i2.52557","DOIUrl":"https://doi.org/10.20473/jscrte.v7i2.52557","url":null,"abstract":"This research is based on refractory angina pectoris, which remains a problem despite advances in coronary heart disease treatment. Stem cell therapy is still in preclinical research stages to address refractory angina. Endothelial progenitor cells (EPCs) aid in improving endothelium and the growth of new blood vessels. Heart medication has shown to enhance both the quantity and function of EPCs in patients at cardiovascular risk or with heart disease. Previous studies reported that ACE inhibitors (ACEI) have a positive effect on EPCs. Thus, this study analyzes the impact of three different ACE inhibitors on EPC migration in laboratory conditions. Its aim is to ascertain the increase in EPC migration in stable coronary heart disease patients after ACEI administration. The research methodology involves an experimental design with a control group and post-treatment assessment only. Mononuclear cells are isolated from stable coronary heart disease patients' peripheral blood and incubated for 3 days. The EPCs are then divided into captopril, ramipril, lisinopril, and a control group, observed for 48 hours. EPC migration is assessed by counting the cells moving from the upper chamber to the membrane facing the lower chamber using a transwell migration assay after 20 hours, observed with a light microscope and Giemsa staining. Data analysis via ANOVA statistical tests indicates increased EPC migration in the captopril, ramipril, and lisinopril groups compared to the control. Captopril shows the highest effect among the groups, while no significant difference is observed between captopril and lisinopril, as well as between ramipril and lisinopril.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139008952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COMPARISON OF INFLAMMATORY RESPONSE BETWEEN PLATELET RICH PLASMA 20% AND AUTOLOGUS SERUM 20% IN EXPERIMENTALLY-INDUCED CORNEAL ALKALI BURN 富血小板血浆20%与自体血清20%实验性角膜碱烧伤炎症反应的比较

Journal of Stem Cell Research and Tissue Engineering Pub Date : 2023-05-26 DOI: 10.20473/jscrte.v7i1.45698

Amelia Amelia Safitri Ramadhani

{"title":"COMPARISON OF INFLAMMATORY RESPONSE BETWEEN PLATELET RICH PLASMA 20% AND AUTOLOGUS SERUM 20% IN EXPERIMENTALLY-INDUCED CORNEAL ALKALI BURN","authors":"Amelia Amelia Safitri Ramadhani","doi":"10.20473/jscrte.v7i1.45698","DOIUrl":"https://doi.org/10.20473/jscrte.v7i1.45698","url":null,"abstract":"Objective to compare the inflammatory response between platelet rich plasma 20% and autologous serum 20% on cornea after sodium hydroxide exposure. Methods a true experimental study with two groups post-test design. A total of 18 rabbits were used in this study and allocated into two groups. After collecting intravenous blood samples from both group, platelet rich plasma 20% and autologous serum 20%, respectively, were obtained by centrifugation and dilution. Alkali burns were inflicted on the central cornea of each rabbit’s right eye by applying a round filter paper, 7.0 mm in diameter, soaked in 1N NaOH for 60 s. Clinical outcome of the inflammation were observed everyday for seven days. On the seventh day, corneal tissue was collected for histopathological examination to evaluate amount of PMN neutrophils that infiltrate the central cornea. All data were statistically analyzed for difference between the study groups. Resuls statistical analysis shows statistically difference in clinical inflammatory feature of conjunctival hyperemia at day 3 until day 7, in which the group that received PRP 20% got lower conjunctival hyperemia grading compared to the group that received AS 20%. Other clinical features, corneal opacity and epithelial defect, shows no statistically difference between two groups. Histopathological examination shows lower amount of PMN neutrophil infiltration to the central cornea on PRP group, compared to AS group. Conclusio Platelet-rich plasma 20% eyedrop can be used as one of adjuvant therapies and has better control of inflammatory response towards alkaline injury of the cornea during acute phase.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85985575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COMPARISON IN CORNEAL COLLAGEN STRUCTURE BETWEEN 20% PLATELET RICH PLASMA AND 20% AUTOLOGOUS SERUM THERAPY POST NATRIUM HIDROKSIDA EXPOSURE 20%富血小板血浆与20%自体血清暴露后角膜胶原结构的比较

Journal of Stem Cell Research and Tissue Engineering Pub Date : 2023-05-26 DOI: 10.20473/jscrte.v7i1.45746

Nila Kurniasari, E. Retnowati, Windhu Purnomo

{"title":"COMPARISON IN CORNEAL COLLAGEN STRUCTURE BETWEEN 20% PLATELET RICH PLASMA AND 20% AUTOLOGOUS SERUM THERAPY POST NATRIUM HIDROKSIDA EXPOSURE","authors":"Nila Kurniasari, E. Retnowati, Windhu Purnomo","doi":"10.20473/jscrte.v7i1.45746","DOIUrl":"https://doi.org/10.20473/jscrte.v7i1.45746","url":null,"abstract":"Objective to compare therapy response between 20% PRP and AS 20% on cornea post sodium hydroxide exposure on collagen structure. Methods this is a true experimental study with post test only design on 18 New Zealand white rabbits. Sample were divided randomly to two groups, each with 9 rabbits. The right eye of each rabbit were exposed to alkali injury with 1N NaOH under general anesthesia. First group was given 20% PRP eyedrops and 20% AS for the second group. And the end of the 7 day periode, all rabbits were euthanized and enucleated to obtain histopthological data. Collagen density, collagen thickness and keratosit cell were evaluated. The result will be analyzed, ratio scale data will be tested with independent T- test, and ordinal scaled data will be tested with Mann-Whitney test. Result The collagen density in the 20% PRP group shows 88.9% grade 2 and 44.4 % grade 2 for the 20% AS group. The collagen thickness in the 20% PRP group is 55.41 and 67.62 for the 20% AS group. The keratosit cell count in the 20% PRP group are 54.56 and 45.47 in the 20% AS group. There is significant difference in the collagen density between 20% PRP and 20% AS treatment (p= 0.066). There is significant difference in the collagen thickness between 20% PRP and 20% AS treatment (p= 0,224). There is significant difference in the keratosit cell count between 20% PRP and 20% AS treatment (p= 0,227). Conclusion From the statistik analysis shows that there is no significant difference in corneal collagen structure between 20% PRP and 20% AS therapy post natrium hidroksida exposure, from our study there is no significant better corneal healing parameters in post alkali chemical ocular injury with 20% PRP eyedrops compare to 20% AS eyedrops.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86334871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ADIPOSE-DERIVED STEM CELL THERAPY ON NON-COMMUNICABLE DISEASE: A SYSTEMATIC REVIEW 非传染性疾病的脂肪来源干细胞治疗:系统综述

Journal of Stem Cell Research and Tissue Engineering Pub Date : 2023-05-26 DOI: 10.20473/jscrte.v7i1.40636

K. Tandarto, Reza Yuridian Purwoko, Caroline Oktarina, Reganedgary Jonlean, C. Irawan, M. Abdullah, Jeanne Adiwinata Pawitan

{"title":"ADIPOSE-DERIVED STEM CELL THERAPY ON NON-COMMUNICABLE DISEASE: A SYSTEMATIC REVIEW","authors":"K. Tandarto, Reza Yuridian Purwoko, Caroline Oktarina, Reganedgary Jonlean, C. Irawan, M. Abdullah, Jeanne Adiwinata Pawitan","doi":"10.20473/jscrte.v7i1.40636","DOIUrl":"https://doi.org/10.20473/jscrte.v7i1.40636","url":null,"abstract":"The increasing number of non-communicable diseases demands practical therapy innovations, including adipose-derived stem cell application. This study aimed to analyze the effectiveness of adipose stem cell therapy on non-communicable disease patients. The method used in this study was a systematic review according to PRISMA 2020 guidelines. The database search was done on PubMed, Google Scholar, Proquest, and the EBSCO host database between 2016 and 2021. ROBINS-I tool and RoB-2 were used to assess the risk of bias in the clinical trial study. The first literature search identified a total of 2615 articles. After exclusion for some reason, 6 articles were included in this systematic review study. A total of five studies were included in this study. Based on the risk of bias assessment of the included studies, it was found that all studies had a low risk of bias in all domains. This study showed that the efficacy of adipose-derived stem cell therapy was inconsistent; however, the results were promising. In addition, the results showed that adipose-derived stem cell therapy was safe without significant side effects. Further study was needed to identify therapeutic strategies based on Evidence-based Medicine (EBM). ","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85223204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BIOSCAFFOLD FROM MOUSE EMBRYONIC FIBROBLAST MAINTAINS THE PLURIPOTENCY OF MOUSE EMBRYONIC STEM CELLS 小鼠胚胎成纤维细胞生物支架维持小鼠胚胎干细胞的多能性

Journal of Stem Cell Research and Tissue Engineering Pub Date : 2023-05-26 DOI: 10.20473/jscrte.v7i1.38140

Rifqah Mifthahul Jannah, A. Naroeni, T. Novianti

{"title":"BIOSCAFFOLD FROM MOUSE EMBRYONIC FIBROBLAST MAINTAINS THE PLURIPOTENCY OF MOUSE EMBRYONIC STEM CELLS","authors":"Rifqah Mifthahul Jannah, A. Naroeni, T. Novianti","doi":"10.20473/jscrte.v7i1.38140","DOIUrl":"https://doi.org/10.20473/jscrte.v7i1.38140","url":null,"abstract":"Cell culture using a 3D method provides Various cell culture strategies have been developed using synthetic or biological materials; most existing publications use many reagents. Bioscaffold from mouse embryonic fibroblast (MEF) enhances cell attachment, interaction, and production of growth factors. Since bioscaffolds could maintain and stimulate pluripotency of stem cells, we conducted this study to prove bioscaffold function. Bioscaffold was prepared from MEF cultured in DMEM complete medium supplemented with dextran sulfate and L-ascorbic acid to increase extracellular matrix production. This medium acts as an embryo stem cell (ESC) culture medium. We used a Tali-cytometer to identify and quantify stem cells based on Sox2 and Oct4 proteins, markers of stemness. ESC culture using bioscaffold maintained the pluripotency of ESC as indicated by the presence of Oct 4 and Sox2 as ESC markers compared to MEF culture. From this research, the bioscaffold from MEF can be developed as media for ESC to improve propagation. Furthermore, it is a model for tissue engineering and in vitro organ development.","PeriodicalId":17049,"journal":{"name":"Journal of Stem Cell Research and Tissue Engineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82581883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

STEM CELL THERAPY IN STROKE 干细胞治疗中风

Journal of Stem Cell Research and Tissue Engineering Pub Date : 2023-05-26 DOI: 10.20473/jscrte.v7i1.36443

Tita Tita Yunia zalni, Tita Tita yunia zalni

引用次数: 0