Journal of Pure and Applied Microbiology最新文献_第5页

A Critical Analysis of Postbiotics: Exploring their Potential Impact on the Health and Food Industries 对后生物的批判性分析:探索其对健康和食品工业的潜在影响

Journal of Pure and Applied Microbiology Pub Date : 2023-11-10 DOI: 10.22207/jpam.17.4.18

A. Surendra Babu, N. Guruprasath, S.A.O. Adeyeye, P. Sankarganesh, A. Ganesh Kumar, T. Sivapriya

引用次数: 0

Seroprevalence and Genotypic Characterization of Orientia tsutsugamushi in Febrile Pediatric Patients Admitted in Tertiary Care Hospital of Chennai, South India 南印度金奈三级医院收治的发热儿童恙虫病东方体的血清患病率和基因型特征

Journal of Pure and Applied Microbiology Pub Date : 2023-11-10 DOI: 10.22207/jpam.17.4.20

Rajagopal Murali, Sivasambo Kalpana, Panneerselvam Satheeshkumar, Prabu Dhandapani

{"title":"Seroprevalence and Genotypic Characterization of Orientia tsutsugamushi in Febrile Pediatric Patients Admitted in Tertiary Care Hospital of Chennai, South India","authors":"Rajagopal Murali, Sivasambo Kalpana, Panneerselvam Satheeshkumar, Prabu Dhandapani","doi":"10.22207/jpam.17.4.20","DOIUrl":"https://doi.org/10.22207/jpam.17.4.20","url":null,"abstract":"Scrub typhus is one of the important vector borne illness which is largely underdiagnosed, particularly in children. It causes mild febrile illness to severe complications. More than 20 genotypic clusters are documented from various geographical regions based on sequence variations of 56kDa type specific antigen gene of Orientia tsutsugamushi, the causative agent of scrub typhus. Adequate knowledge about epidemiology and genetic diversity in endemic regions is an important tool for clinical management, development of diagnostic kit and vaccines. Limited studies are available based on genotypic characterization of Orientia tsutsugamushi in children. The present study determined the prevalence and genotypic characterization of Orientia tsutsugamushi in febrile pediatric patients admitted in tertiary care hospital of Chennai, South India. Both serum and blood samples were collected from 239 scrub typhus suspected febrile pediatric patient’s aged between 6 months to 12 years. IgM ELISA and 56kDa nested PCR were performed on all the patient samples. Nested PCR positive samples were sequenced and analyzed for genotypic differences. Among 239 samples, 103 were positive for IgM ELISA and 35 were positive for nPCR analysis. Out of the 108 scrub typhus positive cases, 45.31% (58/128) were male and 45.05% (50/111) were female. Eschar was positive in 56.48% of patients. Pneumonia (4/108), hypotensive shock (3/108), and myocarditis (1/108) were the most common clinical complications associated with scrub typhus positive children. Karp (56.6%) was the most common genotypic cluster found in our study, followed by TA716 (33.33%), TA763 (2/30), and Gilliam (1/30).","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":" 11","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135141794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Identification of Shimwellia blattae from the Gut of the Hospital Cockroach Blatta orientalis in Annaba City, Algeria 阿尔及利亚安纳巴市医院东方瑛蜚蠊肠内毒舌的分离鉴定

Journal of Pure and Applied Microbiology Pub Date : 2023-11-10 DOI: 10.22207/jpam.17.4.22

Sarah Benouagueni, Asma Labid, Aida Mehainaoui, Djamila Gacemi Kirane

{"title":"Isolation and Identification of Shimwellia blattae from the Gut of the Hospital Cockroach Blatta orientalis in Annaba City, Algeria","authors":"Sarah Benouagueni, Asma Labid, Aida Mehainaoui, Djamila Gacemi Kirane","doi":"10.22207/jpam.17.4.22","DOIUrl":"https://doi.org/10.22207/jpam.17.4.22","url":null,"abstract":"The overall aim of this paper was to divulge the pathogenic and beneficial bacterial flora of cockroaches (Blatta orientalis) living in a hospital area in Annaba city, Algeria. Thirteen cockroaches were randomly apprehended in sterile conditions during March 2022 in two different hospitals in Annaba, “El Bouni” Hospital and the “Sainte-Therese” Hospital. Bacterial strains were isolated from the imprint of the insect on Petri dishes previously inoculated with different media, also from external and internal body part suspensions of the captured specimens. Biochemical identification was established using the analytical profile Index (API) System (Biomerieux, France). The identification of the enteric-screened strain was confirmed using molecular sequencing of the 16S rRNA gene and phylogenetic analysis was performed. The results showed a high prevalence of the pathogenic strains isolated from the oriental cockroach Blatta orientalis (225 strains), such as Serratia liquefaciens, Raoultella ornithinolytica, Pseudomonas luteola, Enterobacter aerogenes, Hafniaalvei and Bacillus sp. Phylogenetic analysis of the enteric bacteria confirmed the affiliation with Shimwellia blattae NCTC10965 (100%), Basonym Escherichia blattae, which is a natural cyanocobalamin producer. Results confirm at the same time the symbiotic relationship between S. blattae and Blatta orientalis, but also point out the underrepresented potential of these insects as a source of strains with biotechnological interest.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":" 9","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135141500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial Susceptibility Pattern of S. Maltophilia Isolates at A Tertiary Care Hospital, India 印度一家三级医院嗜麦芽葡萄球菌分离株的抗菌药敏模式

Journal of Pure and Applied Microbiology Pub Date : 2023-11-10 DOI: 10.22207/jpam.17.4.23

Mohammed Ashraf Ali Namaji, Muzafar Ahmad Bhat, Manas Dixit, Sanjay Pratap Singh, Raghavendra Huchchannavar

{"title":"Antibacterial Susceptibility Pattern of S. Maltophilia Isolates at A Tertiary Care Hospital, India","authors":"Mohammed Ashraf Ali Namaji, Muzafar Ahmad Bhat, Manas Dixit, Sanjay Pratap Singh, Raghavendra Huchchannavar","doi":"10.22207/jpam.17.4.23","DOIUrl":"https://doi.org/10.22207/jpam.17.4.23","url":null,"abstract":"A sudden emergence of Stenotrophomonas maltophilia as a primary pathogen both in immunocompromised and immunocompetent individuals has raised a serious concern, as it is associated with significant case fatality ratio. We intended to study the clinico-microbiological profile of S. maltophilia isolates from various samples and outcome of the infections in a tertiary healthcare center, Pune, India. This is an observational cross-sectional study was conducted from January 2021 to June 2022 at Department of Microbiology of a tertiary care Centre in Pune, India. Of the 12049 samples received for culture, S. maltophilia was isolated in 57 samples. Only 42 samples with pure growth of S. maltophilia were included in the study with 15 excluded due to mixed growth. All isolates were confirmed by VITEK-MS (bioMerieux, SA, France) which uses Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) technology. Of the 42 isolates, majority were isolated from pus(28.6%) and most of patients (61.9%) were from acute health care settings. The isolates had high susceptibility to Cotrimoxazole (85.7%) and Minocycline (85.7%) and low susceptibility to Ceftazidime (45.2%). A case fatality rate of 7.1% (3/42 cases) was noted and 39 cases were discharged after complete treatment. All the three fatal cases were susceptible to levofloxacin, ciprofloxacin, cotrimoxazole and minocycline and all three fatal cases were resistant to ceftazidime. S. maltophilia has recently shown an increase in nosocomial infections especially in acute healthcare settings like ICU and other critical care wards. The isolates of the present study had high susceptibility to trimethoprim-sulfamethoxazole (TMP-SXT) and Minocycline and low susceptible to Ceftazidime.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":" 7","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135141315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Identification of Green Microalgae from Northern Jordan 约旦北部绿色微藻的分离与鉴定

Journal of Pure and Applied Microbiology Pub Date : 2023-11-01 DOI: 10.22207/jpam.17.4.17

Wafaa Odeh, Mais Sweiss, Fatima Haj Ahmad, Zeinab Arabeyyat, Waed Alnsour, Marah Aldabbas, Maen Hasan

{"title":"Isolation and Identification of Green Microalgae from Northern Jordan","authors":"Wafaa Odeh, Mais Sweiss, Fatima Haj Ahmad, Zeinab Arabeyyat, Waed Alnsour, Marah Aldabbas, Maen Hasan","doi":"10.22207/jpam.17.4.17","DOIUrl":"https://doi.org/10.22207/jpam.17.4.17","url":null,"abstract":"In light of the rapid and extreme changes in climate and the steady depletion of natural resources, there is an urgent need to find innovative and sustainable solutions to these problems. Microorganisms such as microalgae can offer viable solutions to these challenges. Proper investment in such organisms requires the identification of the algal species that inhabit the region. Therefore, this study aimed to isolate and molecularly characterize green microalgae that inhabit freshwater at different locations in the governorates of Irbid and Ajloun in the northern region of Jordan. Water samples collected from these regions were used to isolate single colonies, some of which exhibited different morphological characteristics. Genomic DNA was extracted from the isolates and used as a template for PCR amplification of the 18S ribosomal DNA gene (18S rDNA) and the internal transcribed spacer (ITS) region. Phylogenetic trees were constructed based on 18S rDNA and ITS PCR product sequences, which were used to identify the isolates at the genus level. The obtained isolates belonged to three genera; Coelastrella, Desmodesmus, and Monoraphidium. The latter species has not been previously reported in Jordan.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"262 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135320505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of the Topical Application of 4% Chlorhexidine on Umbilical Cord Bacterial Colonization – A Non-Randomized Control Study 局部应用4%氯己定对脐带细菌定植的有效性——一项非随机对照研究

Journal of Pure and Applied Microbiology Pub Date : 2023-11-01 DOI: 10.22207/jpam.17.4.15

B.P. Prasanna, Kiran Baliga, K. Vishwas Saralaya, P. Anupama Nayak, S.R. Ravikiran

{"title":"Effectiveness of the Topical Application of 4% Chlorhexidine on Umbilical Cord Bacterial Colonization – A Non-Randomized Control Study","authors":"B.P. Prasanna, Kiran Baliga, K. Vishwas Saralaya, P. Anupama Nayak, S.R. Ravikiran","doi":"10.22207/jpam.17.4.15","DOIUrl":"https://doi.org/10.22207/jpam.17.4.15","url":null,"abstract":"Newborns have the highest risk of death in the first 4 weeks of their life. Umbilical cord of a child is an important site for microorganism colonization and also forms a portal of entry for invasive pathogens. This study focuses on the bacterial colonization with 2 separate cord care regimens: dry cord and 4% chlorhexidine care group. This is a randomized control time-bound study conducted at a tertiary health care centre in Mangalore. A total of 144 newborns fulfilling the inclusion criteria were included and then divided randomly into two groups: dry cord care and 4% chlorhexidine. Two swabs were collected, baseline swab (swab 1) within 3 hours of birth and second swab (swab 2) at 120± 10 hours of birth and these samples were cultured for gram negative and positive organisms. Results were evaluvated with Chi-Square test. Out of 144 samples, in 115 showed no growth at the baseline and only 29 showed growth. In the dry cord care group, 38.9% showed growth of microorganism in comparison with chlorhexidine group which was 1.4% in swab 1 (baseline) and in swab 2, 87.5% in dry cord care group and 18.1% in chlrohexidine group in swab 2 respectively. It was observed that methicilin susceptible Staphylococcus aureus (MSSA) and methicillin resistant Staphylococcus aureus (MRSA) were the most common microorganisms isolated with others being Pseudomonas species, coagulase-negative Staphylococci (CoNS), Enterobacter species and Citrobacter species. Use of 4% chlorhexidine is effective in reducing colonization of bacteria and infection in comparison with dry cord care method. Therefore, decontamination of the umbilical cord with 4% of chlorhexidine should be recommended in a newborn child. However, both cord care methods did not lead to any infections.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"272 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135320681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Detection of Key Virulence-associated Genes and Phenotypic Analysis of Virulence Traits of Klebsiella pneumoniae Clinical Isolates from Kenya 肯尼亚肺炎克雷伯菌临床分离株关键毒力相关基因的分子检测及毒力性状的表型分析

Journal of Pure and Applied Microbiology Pub Date : 2023-11-01 DOI: 10.22207/jpam.17.4.16

Fredrick Tiria, Erick Odoyo, Martin Georges, Andrew Nyerere, Lillian Musila

{"title":"Molecular Detection of Key Virulence-associated Genes and Phenotypic Analysis of Virulence Traits of Klebsiella pneumoniae Clinical Isolates from Kenya","authors":"Fredrick Tiria, Erick Odoyo, Martin Georges, Andrew Nyerere, Lillian Musila","doi":"10.22207/jpam.17.4.16","DOIUrl":"https://doi.org/10.22207/jpam.17.4.16","url":null,"abstract":"Klebsiella pneumoniae is an opportunistic pathogen and a major cause of nosocomial infections. Phenotypic analysis of virulence and molecular typing of virulence-associated genes are powerful approaches to understanding Klebsiella pneumoniae infection biology. This study subjected 102 clinical Klebsiella pneumoniae isolates to virulence gene screening and phenotypic analysis of serum resistance, biofilm formation, and hypermucoviscosity. The virulence genes mrkD, ybtS, wcaJ, entD, and rmpA had a prevalence of 95.1%, 30.4%, 27.5%, 22.5%, and 0.98%, respectively. 54.9%, 36.3%, and 8.8% were serum resistant, intermediate, and susceptible, respectively. There was no significant correlation between the presence of mrkD, ybtS, wcaJ, entD, and rmpA genes and serum non-susceptibility. 53.9%, 22.5%, 6.9%, and 16.7% were strong, moderate, weak, and non-biofilm formers, respectively. The biofilm-forming phenotype was significantly correlated with mrkD (P= 0.000098) and ybtS (P=0.032) gene presence. In addition, 11.8 % of the isolates had the hypermucoviscous phenotype indicating hypervirulence. All of these hypervirulent isolates were positive for the mrkD gene and were significantly associated with the presence of the wcaJ gene (P = 0.000085). These results indicate a positive association between virulence genes with biofilm formation and hypervirulence. In conclusion, Klebsiella pneumoniae isolates circulating in Kenya are predominantly serum non-susceptible and biofilm formers. mrkD, ybtS, and wcaJ genes were identified as key genes influencing biofilm formation and hypervirulence and would be good targets for vaccine development to reduce the severity of Klebsiella pneumoniae infections in Kenya.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"1 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135272836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial Susceptibility Pattern and Prevalence of the Emerging Nosocomial Pathogen Achromobacter xylosoxidans in a North Indian Tertiary Care Hospital 北印度一家三级医院新出现的医院病原菌木糖氧化无色杆菌的药敏模式和流行

Journal of Pure and Applied Microbiology Pub Date : 2023-10-23 DOI: 10.22207/jpam.17.4.11

Malvika Singh, Dimple Raina, Ranjana Rohilla, Himanshu Narula, Ajay Pandita

{"title":"Antimicrobial Susceptibility Pattern and Prevalence of the Emerging Nosocomial Pathogen Achromobacter xylosoxidans in a North Indian Tertiary Care Hospital","authors":"Malvika Singh, Dimple Raina, Ranjana Rohilla, Himanshu Narula, Ajay Pandita","doi":"10.22207/jpam.17.4.11","DOIUrl":"https://doi.org/10.22207/jpam.17.4.11","url":null,"abstract":"Achromobacter xylosoxidans is an emerging nosocomial pathogen which is commonly found in the environment. In hospital settings, especially in ICU, it can be a cause of nosocomial infection. It is commonly found in the humidifiers in ICU settings and it is also commonly associated with the immunocompromised state of patient having comorbidities. The objective of the study was to study the prevalence of Achromobacter xylosoxidans and its antimicrobial sensitivity pattern. The Retrospective analysis was done of the culture reports positive for Achromobacter xylosoxidans by VITEK 2 method and its Antimicrobial sensitivity pattern was analysed from the period of September 2021 to February 2023.The maximum (54.54%) infection was seen in the age group >50 years. The maximum number (66.2%) of Achromobacter xylosoxidans were isolated from Suction tip, followed by blood (8%) and Tracheal Tip (5%). Surgical ICU contributed to the maximum number of infections i.e. 40.2%, followed by Respiratory ICU (22.1%). Maximum sensitivity was seen for Cotrimoxazole and Meropenem (around 80%), followed by Cefoperazone-Sulbactam (74%), Imipenem, Levofloxacin, Ceftazidime (around 65%). The sensitivity was minimal for Ceftriaxone (0%), Aztreonam (1.3%), and Gentamicin (5.2%). The most common risk factors/ comorbidities associated with Achromobacter infections was recent ICU admission (87.01%). The antibiotic sensitivity trends to all the antibiotics used, declined from 2021 to 2022. The antibiotic of choice to our conclusion is Cotrimoxazole, followed by Piperacillin-Tazobactam. Colistin should be kept as a reserve drug for the last resort treatment. The bacteria should not be ignored as it can lead to various opportunistic infections in immunocompromised patients, causing hindrance in the treatment.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135411654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing hs-CRP and MPO Biomarkers of subclinical Atherosclerosis in Metabolic Syndrome Patients 代谢综合征亚临床动脉粥样硬化患者hs-CRP与MPO生物标志物的比较

Journal of Pure and Applied Microbiology Pub Date : 2023-10-23 DOI: 10.22207/jpam.17.4.12

Abishkauf Jenish Beautlin, Ashok Govindaraj, Durga Devi, Gurubharath Ilangovan, Divya Lakshmi

{"title":"Comparing hs-CRP and MPO Biomarkers of subclinical Atherosclerosis in Metabolic Syndrome Patients","authors":"Abishkauf Jenish Beautlin, Ashok Govindaraj, Durga Devi, Gurubharath Ilangovan, Divya Lakshmi","doi":"10.22207/jpam.17.4.12","DOIUrl":"https://doi.org/10.22207/jpam.17.4.12","url":null,"abstract":"Cardiovascular disorders have been linked to myeloperoxidase, an enzyme generated from leukocytes. MPO creates an antibacterial system and has been many correlations between MPO, hs-CRP and cardiovascular disease. The present study has aimed to assess MPO, hs-CRP and subclinical atherosclerosis in metabolic syndrome patients by using FMD and CIMT parameters. 75 metabolic syndrome affected patients had defined as per the criteria of IDF (International Diabetes Federation) which had been in this study. In addition, ultrasound doppler had been employed for determining the carotid intima medial thickness for both right and left (left and right CIMT) and brachial artery FMD. By employing the Immunoassay MPO and hs-CRP ultrasensitive ELISA kit, the concentration of MPO and hs-CRP is measured. A positive link between MPO with CIMT right and left (r value of right CIMT=0.723, p <0.05 and left CIMT r=0.712, p<0.01), hs-CRP with CIMT right and left (r value of right CIMT=0.613, p<0.05and left CIMT r=0.64, p<0.01)and a significant inverse correlation between MPO with FMD (r= -0.319,p<0.05), hs-CRP with FMD (r= -0.304,p<0.01) in metabolic syndrome patients. Serum MPO and hs-CRP is positively linked with subclinical atherosclerosis in metabolic syndrome patients. According to this finding, understanding the mechanism behind the risk factors for cardiovascular disease can be aided by the development of new molecular markers for metabolic syndrome and subclinical atherosclerosis.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135366345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Nested PCR and Loop-Mediated Isothermal Amplification (LAMP) to Target 56kDa gene in Scrub Typhus Patients and Phylogenetic Analysis to Identify Orientia tsutsugamushi Strains Circulating In and Around Puducherry 应用巢式PCR和环介导等温扩增(LAMP)技术检测恙虫病56kDa基因及系统发育分析鉴定普度切里市及周边地区流行的恙虫病东方体

Journal of Pure and Applied Microbiology Pub Date : 2023-10-23 DOI: 10.22207/jpam.17.4.09

V. Anitharaj, J. Pradeep, S. Amsaveni, Selvaraj Stephen, Pooja Pratheesh

{"title":"Application of Nested PCR and Loop-Mediated Isothermal Amplification (LAMP) to Target 56kDa gene in Scrub Typhus Patients and Phylogenetic Analysis to Identify Orientia tsutsugamushi Strains Circulating In and Around Puducherry","authors":"V. Anitharaj, J. Pradeep, S. Amsaveni, Selvaraj Stephen, Pooja Pratheesh","doi":"10.22207/jpam.17.4.09","DOIUrl":"https://doi.org/10.22207/jpam.17.4.09","url":null,"abstract":"Scrub typhus (ST) is a re-emerging zoonotic disease caused by Orientia tsutsugamushi and is transmitted by chiggers. Serological tests targeting IgM and/or IgG antibodies play a major role in the detection of ST cases. Orientia 56kDa genome is common target for the molecular diagosis of ST to identify the prevalence of specific serotypes of O. tsutsugamushi in and around Puducherry by targeting 56kDa gene with the application of phylogenetic analysis. This prospective laboratory-based study was conducted in a tertiary care teaching hospital, from November 2105 to March 2018. Blood samples were collected from out-patients/in-patients, and those tested positive for ST IgM ELISA (n=140) and an equal number of negative samples were archived and anonymized. Genomic DNA was extracted and analyzed by using Nested PCR and LAMP assay. The positive products were purified and sequenced. Phylogenetic tree was constructed based on the sequences. Among 280 samples, 45 (16.1%) N-PCR and 102 LAMP (36.43%) positivity was observed for 56kDa gene. Forty-one N-PCR positive products were sequenced and accession numbers were obtained (MG601875 to MG601917) for the isolates. Phylogenetic analysis was done by Maximum Likelihood methods and this study has showed that 32.3% are similar to the Karp isolates. Molecular diagnosis of Scrub typhus has become essential in case of doubtful serology and early acute phase of illness. Gene sequencing result indicates that most of them were different from the existing ones, which may belong to the newer strains. The identification of newer strains will be helpful in future for development of scrub typhus vaccine.","PeriodicalId":16968,"journal":{"name":"Journal of Pure and Applied Microbiology","volume":"1 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135411663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0