Journal of Receptors and Signal Transduction最新文献_第8页

Emodin alleviates high glucose-induced oxidative stress, inflammation and extracellular matrix accumulation of mesangial cells by the circ_0000064/miR-30c-5p/Lmp7 axis. 大黄素通过circ_0000064/miR-30c-5p/Lmp7轴缓解高糖诱导的系膜细胞氧化应激、炎症和细胞外基质积累。

IF 2.8 4区生物学

Journal of Receptors and Signal Transduction Pub Date : 2022-06-01 Epub Date: 2021-06-21 DOI: 10.1080/10799893.2021.1933028

Li Sun, Yanquan Han, Chuqiao Shen, Huan Luo, Zhuo Wang

{"title":"Emodin alleviates high glucose-induced oxidative stress, inflammation and extracellular matrix accumulation of mesangial cells by the circ_0000064/miR-30c-5p/Lmp7 axis.","authors":"Li Sun, Yanquan Han, Chuqiao Shen, Huan Luo, Zhuo Wang","doi":"10.1080/10799893.2021.1933028","DOIUrl":"https://doi.org/10.1080/10799893.2021.1933028","url":null,"abstract":"Emodin has been shown to exert a renoprotective effect in diabetic nephropathy (DN). In this paper, we investigated whether circular RNAs (circRNAs) might be involved in the renoprotective mechanism of emodin in DN. The levels of malondialdehyde (MDA), reactive oxygen species (ROS), superoxide dismutase (SOD), interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α) were measured using the corresponding assay kits. The expression levels of circ_0000064, microRNA (miR)-30c-5p, large multifunctional protease 7 (Lmp7), fibronectin (FN), and collagen type I (Col.1) were gauged by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. Subcellular localization assay was used to assess the cellular localization of circ_0000064. Targeted relationships among circ_0000064, miR-30c-5p and Lmp7 were confirmed by dual-luciferase reporter, RNA pull-down and RNA immunoprecipitation (RIP) assays. Our data showed the alleviative effect of emodin on HG-induced oxidative stress, inflammation and extracellular matrix (ECM) accumulation in SV-MES13 cells. Circ_0000064 was an importantly downstream effector of emodin function in HG-induced SV40-MES13 cells. Moreover, circ_0000064 directly targeted miR-30c-5p, and circ_0000064 modulated Lmp7 expression through miR-30c-5p. Circ_0000064 silencing alleviated HG-induced cell oxidative stress, inflammation and ECM accumulation via up-regulating miR-30c-5p. The enforced expression of miR-30c-5p attenuated HG-induced oxidative stress, inflammation and ECM accumulation in SV40-MES13 cells by targeting Lmp7. Our findings identified that emodin alleviated HG-induced oxidative stress, inflammation and ECM accumulation in SV40-MES13 cells at least partially by the regulation of the circ_0000064/miR-30c-5p/Lmp7 axis.","PeriodicalId":16962,"journal":{"name":"Journal of Receptors and Signal Transduction","volume":"42 3","pages":"302-312"},"PeriodicalIF":2.8,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10799893.2021.1933028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39251005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Baicalin attenuates XRCC1-mediated DNA repair to enhance the sensitivity of lung cancer cells to cisplatin. 黄芩苷可减弱xrcc1介导的DNA修复，增强肺癌细胞对顺铂的敏感性。

IF 2.8 4区生物学

Journal of Receptors and Signal Transduction Pub Date : 2022-06-01 Epub Date: 2021-03-15 DOI: 10.1080/10799893.2021.1892132

Zhangyong Yin, Enguo Chen, Xiaoping Cai, Enhui Gong, Yuling Li, Cunlai Xu, Zaiting Ye, Zhuo Cao, Jiongwei Pan

{"title":"Baicalin attenuates XRCC1-mediated DNA repair to enhance the sensitivity of lung cancer cells to cisplatin.","authors":"Zhangyong Yin, Enguo Chen, Xiaoping Cai, Enhui Gong, Yuling Li, Cunlai Xu, Zaiting Ye, Zhuo Cao, Jiongwei Pan","doi":"10.1080/10799893.2021.1892132","DOIUrl":"https://doi.org/10.1080/10799893.2021.1892132","url":null,"abstract":"Baicalin plays important roles in different types of cancer. A previous report showed that baicalin attenuates cisplatin resistance in lung cancer. However, its mechanism remains unclear. In this study, we investigated the effect and mechanism of baicalin on DNA repair and sensitivity of lung cancer cells to cisplatin. A549 and A549/DPP cells were treated with baicalin and cisplatin. A549/DPP cells were transfected with XRCC1 and siXRCC1. Cell viability and DNA damage were detected by MTT and comet assay. Apoptosis rate and cell cycle were detected by flow cytometry assay. The expressions of Bax, Bcl-2, and Cyclin D1 were detected by western blot. XRCC1 expression was detected by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and western blot. Baicalin and cisplatin decreased cell viability in A549 and A549/DPP cells in dose-dependent manner. Baicalin enhanced the effect of cisplatin on promoting apoptosis, arresting cell on S stage and triggering DNA damage accompanied with the upregulation of Bcl-2-associated X protein (Bax) and downregulation of B-cell lymphoma 2 (Bcl-2) and Cyclin D1 in A549/DPP cells. Moreover, baicalin promoted the inhibitory effect of cisplatin on XRCC1 expression in A549 and A549/DPP cells. However, the synthetic effects of baicalin and cisplatin on A549/DPP cells were partially inhibited by XRCC1 overexpression and promoted by XRCC1 knockdown. This study demonstrates that baicalin interferes with XRCC1-mediated cellar DNA repair to sensitize lung cancer cells to cisplatin.","PeriodicalId":16962,"journal":{"name":"Journal of Receptors and Signal Transduction","volume":"42 3","pages":"215-224"},"PeriodicalIF":2.8,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10799893.2021.1892132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25478666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Selective thyroid hormone receptor beta agonist, GC-1, is capable to reduce growth of colorectal tumor in syngeneic mouse models 选择性甲状腺激素受体激动剂GC-1在同基因小鼠模型中能够抑制结直肠肿瘤的生长

IF 2.8 4区生物学

Journal of Receptors and Signal Transduction Pub Date : 2022-04-27 DOI: 10.1080/10799893.2022.2032748

K. Pourvali, Ghazaleh Shimi, Arman Ghorbani, Azam Shakery, F. Shirazi, H. Zand

{"title":"Selective thyroid hormone receptor beta agonist, GC-1, is capable to reduce growth of colorectal tumor in syngeneic mouse models","authors":"K. Pourvali, Ghazaleh Shimi, Arman Ghorbani, Azam Shakery, F. Shirazi, H. Zand","doi":"10.1080/10799893.2022.2032748","DOIUrl":"https://doi.org/10.1080/10799893.2022.2032748","url":null,"abstract":"Abstract Objective The effect of thyroid hormone (TH) on cancers was proposed more than 100 years ago; however, conclusions are conflicting. THs are precisely regulated at tissue and cellular levels. It seems that this regulation is altered in cancers. Thyroid hormone receptor beta (TRβ) has anti-proliferative and tumor-suppressive effects in many cancer cells. Therefore, we decided to investigate thyroid hormone receptor beta (THRB) expression and activation by the selective agonist, GC-1, on tumor growth in a syngeneic mouse model of colorectal cancer (CRC) and colon cell lines. Methods In vitro cell viability assay using MTT analysis, cell cycle analysis by PI staining, and FACS analysis were performed. In vivo tumor growth measurements were carried out by caliper and [18F] Fluoro-2-deoxy-2-D-glucose (FDG) – PET imaging. Gene expressions were determined using quantitative-PCR. Results Some concentrations of GC-1 had a marked negative effect on the cell viability of colorectal cell lines. Cell cycle analysis showed that the anti-proliferative effect of GC-1 may not result from cell cycle arrest or apoptosis. Tumor growth analysis in mice harboring colorectal tumor showed that GC-1 treatment for 8 d profoundly inhibited tumor growth and 18FDG uptake. THRB expression was decreased in mice tumor; however, it was upregulated following GC-1 administration. Conclusions Our results showed that specific activation of TRβ by GC-1 had negative effect on tumor growth and restored its gene expression in tumors of CRC mice model.","PeriodicalId":16962,"journal":{"name":"Journal of Receptors and Signal Transduction","volume":"4 1","pages":"495 - 502"},"PeriodicalIF":2.8,"publicationDate":"2022-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82822624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Upregulation of SDHA inhibited proliferation, migration, and invasion of clear cell renal cell carcinoma cells via inactivation of the Wnt/β-catenin pathway. SDHA的上调通过抑制Wnt/β-catenin通路的失活，抑制透明细胞肾细胞癌细胞的增殖、迁移和侵袭。

IF 2.8 4区生物学

Journal of Receptors and Signal Transduction Pub Date : 2022-04-01 Epub Date: 2021-02-18 DOI: 10.1080/10799893.2021.1883060

Xiaolong Xu, Naiwei Zhang, Ruxu Gao, Jianfeng Wang, Zhihong Dai, Jianbin Bi

{"title":"Upregulation of SDHA inhibited proliferation, migration, and invasion of clear cell renal cell carcinoma cells via inactivation of the Wnt/β-catenin pathway.","authors":"Xiaolong Xu, Naiwei Zhang, Ruxu Gao, Jianfeng Wang, Zhihong Dai, Jianbin Bi","doi":"10.1080/10799893.2021.1883060","DOIUrl":"https://doi.org/10.1080/10799893.2021.1883060","url":null,"abstract":"Clear cell renal cell carcinoma (ccRCC) is a common genitourinary malignancy with high mortality. Recent findings suggest that the succinate dehydrogenase complex subunit A (SDHA) is lowly expressed in many types of cancers and involved in tumorigenesis. However, the potential regulatory roles and molecular mechanisms by which SDHA affects the development and progression of ccRCC remain largely unknown. In this study, our results showed that there was significant downregulation of SDHA in ccRCC tissue relative to corresponding non-cancerous tissue, and low expression of SDHA was associated with Fuhrman pathological grade, tumor size, TNM stage, metastasis, and poor prognosis in ccRCC patients. Moreover, overexpression of SDHA inhibited the proliferation, invasion, and migration capacities of ccRCC cells. Mechanistically, SDHA impeded the proliferation and metastasis of ccRCC cells by inactivation of the Wnt/β-catenin pathway. In vivo experiments, SDHA suppressed ccRCC growth in a nude mouse model. In conclusion, our study results indicated that SDHA may act as a new molecular marker for judging the occurrence and development of ccRCC and serve as a therapeutic target for the treatment of human ccRCC.","PeriodicalId":16962,"journal":{"name":"Journal of Receptors and Signal Transduction","volume":"42 2","pages":"180-188"},"PeriodicalIF":2.8,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10799893.2021.1883060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25380914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Catestatin enhances ATP-induced activation of glial cells mediated by purinergic receptor P2X₄. Catestatin增强嘌呤能受体P2X4介导的atp诱导的胶质细胞活化。

IF 2.8 4区生物学

Journal of Receptors and Signal Transduction Pub Date : 2022-04-01 Epub Date: 2021-01-27 DOI: 10.1080/10799893.2021.1878536

Errong Du, Anhui Wang, Rongping Fan, Lilou Rong, Runan Yang, Juping Xing, Xiangchao Shi, Bao Qiao, Ruoyang Yu, Changshui Xu

{"title":"Catestatin enhances ATP-induced activation of glial cells mediated by purinergic receptor P2X4.","authors":"Errong Du, Anhui Wang, Rongping Fan, Lilou Rong, Runan Yang, Juping Xing, Xiangchao Shi, Bao Qiao, Ruoyang Yu, Changshui Xu","doi":"10.1080/10799893.2021.1878536","DOIUrl":"https://doi.org/10.1080/10799893.2021.1878536","url":null,"abstract":"The activation of glial cells and its possible mechanism play an extremely important role in understanding the pathophysiological process of some clinical diseases, and catestatin (CST) is involved in regulating this activation. In this project, we found that CST could enhance the activation of satellite glial cells (SGCs) and microglial cells and that the expression of P2X4 was increased; the co-expression of the P2X4 receptor with glial fibrillary acidic protein (GFAP) and the P2X4 receptor with CD11b was also increased significantly in glial cells of the ATP + CST group, and TNF-α and IL-1β also showed a rising trend; the expression of phosphorylated ERK1/2 was also increased in the ATP + CST group. In summary, we conclude that CST could enhance ATP-induced activation of SGCs and microglial cells mediated by the P2X4 receptor and that the ERK1/2 signaling pathway may be involved in this activation process.","PeriodicalId":16962,"journal":{"name":"Journal of Receptors and Signal Transduction","volume":"42 2","pages":"160-168"},"PeriodicalIF":2.8,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10799893.2021.1878536","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38867276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

UBE2T promotes proliferation, invasion and glycolysis of breast cancer cells by regualting the PI3K/AKT signaling pathway. UBE2T通过调控PI3K/AKT信号通路促进乳腺癌细胞的增殖、侵袭和糖酵解。

IF 2.8 4区生物学

Journal of Receptors and Signal Transduction Pub Date : 2022-04-01 Epub Date: 2021-01-12 DOI: 10.1080/10799893.2020.1870495

Lei Qiao, Chao Dong, Binlin Ma

{"title":"UBE2T promotes proliferation, invasion and glycolysis of breast cancer cells by regualting the PI3K/AKT signaling pathway.","authors":"Lei Qiao, Chao Dong, Binlin Ma","doi":"10.1080/10799893.2020.1870495","DOIUrl":"https://doi.org/10.1080/10799893.2020.1870495","url":null,"abstract":"Purpose: Breast cancer (BCa) is one of the most common gynecological malignancies. Ubiquitin-coupled enzyme E2T (UBE2T) has been demonstrated to play crucial roles in various tumors.Methods: UBE2T levels were detected using quantitative real time PCR and western blot. CCK-8 and colony formation assays were used to evaluate cell proliferation. A xenograft model was used to evaluate the effects of UBE2T on tumor growth in mice, and immunohistochemistry (IHC) assay was performed to detect the expression of UBE2T and Ki-67. Transwell assay was performed to determine cell migration and invasion. The ATP level, glucose consumption and lactate production were measured using the corresponding commercial kits. Western blot assay was used to detect the levels of epithelial-mesenchymal transformation (EMT), glycolytic and the PI3K/AKT pathway related proteins regulated by UBE2T.Results: Upregulation of UBE2T expression in human BCa tissues was found in human clinical BCa tissues and The Cancer Genome Atlas (TCGA) dataset. The expression of UBE2T was confirmed to be up-regulated in BCa cells compared to normal breast epithelial cell line (MCF-10A). Overexpression of UBE2T promoted proliferation, migration, invasion and glycolysis in BCa cells, while UBE2T knockdown showed the opposite results. Moreover, UBE2T knockdown suppressed tumor growth in mice. Further mechanism analysis shows that UBE2T participated in the regulation of BCa progression through affecting the PI3K/AKT signaling pathway.Conclusion: UBE2T promoted proliferation, invasion and glycolysis through modulating PI3K/AKT signaling pathway in BCa, implying that UBE2T may provide a promising therapeutic target for the therapy of BCa.","PeriodicalId":16962,"journal":{"name":"Journal of Receptors and Signal Transduction","volume":"42 2","pages":"151-159"},"PeriodicalIF":2.8,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10799893.2020.1870495","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38811443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Silencing of circular RNA PUM1 inhibits clear cell renal cell carcinoma progression through the miR-340-5p/FABP7 axis. 沉默环状RNA PUM1通过miR-340-5p/FABP7轴抑制透明细胞肾细胞癌的进展。

IF 2.8 4区生物学

Journal of Receptors and Signal Transduction Pub Date : 2022-04-01 Epub Date: 2021-01-20 DOI: 10.1080/10799893.2020.1870494

Fanchang Zeng, Liumei Luo, Mi Song, Daoyuan Li

{"title":"Silencing of circular RNA PUM1 inhibits clear cell renal cell carcinoma progression through the miR-340-5p/FABP7 axis.","authors":"Fanchang Zeng, Liumei Luo, Mi Song, Daoyuan Li","doi":"10.1080/10799893.2020.1870494","DOIUrl":"https://doi.org/10.1080/10799893.2020.1870494","url":null,"abstract":"Circular RNAs (circRNAs) monitor the development of clear cell renal cell carcinoma (ccRCC). However, the role of CircPUM1 in ccRCC malignancy is not studied. We estimated the mechanism of CircPUM1 in ccRCC progression in this study. CircPUM1 expression in ccRCC tissues and cells was detected. The expression of CircPUM1 was interfered in ccRCC cells, and its effects on the growth of ccRCC cells were studied. Nuclear/cytosol fractionation assay was performed for the location of CircPUM1, and the downstream miR, gene, and pathway involved in ccRCC progression were explored through gain- and loss-of-function experiments. CircPUM1 was highly expressed in ccRCC samples and cells. Inhibition of CircPUM1 prevented the growth ccRCC cells. CircPUM1 was localized in the cytoplasm and bound to miR-340-5p. Overexpression of miR-340-5p inhibited the growth of ccRCC cells. miR-340-5p targeted FABP7, and CircPUM1 induced FABP7 expression and the activation of MEK/ERK pathway through competitively binding to miR-340-5p. Overexpression of FABP7 attenuated the inhibitory effect of CircPUM1 silencing on the growth of ccRCC cells. Overall, CircPUM1 upregulates FABP7 expression by competitively binding to miR-340-5p, and then activates the MEK/ERK pathway, thus promoting ccRCC progression.","PeriodicalId":16962,"journal":{"name":"Journal of Receptors and Signal Transduction","volume":"42 2","pages":"141-150"},"PeriodicalIF":2.8,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10799893.2020.1870494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38842066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

miR-183/TMSB4Y, a new potential signaling axis, involving in the progression of laryngeal cancer via modulating cell adhesion. miR-183/TMSB4Y，一个新的潜在信号轴，通过调节细胞粘附参与喉癌的进展。

IF 2.8 4区生物学

Journal of Receptors and Signal Transduction Pub Date : 2022-04-01 Epub Date: 2020-12-27 DOI: 10.1080/10799893.2020.1863987

Bin Lu, Ying Yu, Xiao-Ling Xing, Rui-Yue Liu

{"title":"miR-183/TMSB4Y, a new potential signaling axis, involving in the progression of laryngeal cancer via modulating cell adhesion.","authors":"Bin Lu, Ying Yu, Xiao-Ling Xing, Rui-Yue Liu","doi":"10.1080/10799893.2020.1863987","DOIUrl":"https://doi.org/10.1080/10799893.2020.1863987","url":null,"abstract":"Laryngeal cancer (LCa) is a prevalent malignant head and neck cancer with relatively unclear pathogenesis. A prior study has suggested that miR-183 differentially expressed in laryngeal-related malignancies, but its accurate role has not been fully ascertained in LCa. miR-183 expression in LCa tissues and cells was detected assisted by TCGA/GEO databases or qRT-PCR assay, relatively. Target genes of miR-183 were predicted via accessing to TargetScan website. Luciferase activity analysis was conducted to determine the relationship between miR-183 and its possible target. CCK-8, colony formation and transwell invasion and migration experiments were implemented to measure LCa cell viability, invasion and migration. Western blot assay was utilized to evaluate cell adhesion and EMT-related proteins expressions. The expression of miR-183 was expressed in LCa tissue samples and cells at higher levels than normal controls. Upregulation of miR-183 facilitated Hep-2 and TU212 cells viability, while miR-183 reduction inhibited the proliferative potential of Hep-2 and TU212 cells. TMSB4Y was determined as a possible target of miR-183, and its expression was decreased in LCa. LCa patients with low TMSB4Y expression had poorer outcomes relative to that with high TMSB4Y expression. TMSB4Y overturned the promoting impacts of miR-183 on the LCa cellular malignant behaviors, including cell proliferation, colonogenicity, invasion and migration. miR-183 overexpression inhibited cell adhesion through inhibiting TMSB4Y expression. Overall, all results elucidated that miR-183, as an oncogenic molecule in LCa, may be used to predict the prognosis of LCa patients by targeting TMSB4Y.","PeriodicalId":16962,"journal":{"name":"Journal of Receptors and Signal Transduction","volume":"42 2","pages":"133-140"},"PeriodicalIF":2.8,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10799893.2020.1863987","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38745399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Plantamajoside protects H9c2 cells against hypoxia/reoxygenation-induced injury through regulating the akt/Nrf2/HO-1 and NF-κB signaling pathways. 车前草皂苷通过调节akt/Nrf2/HO-1和NF-κB信号通路，保护H9c2细胞免受缺氧/再氧化诱导的损伤。

IF 2.8 4区生物学

Journal of Receptors and Signal Transduction Pub Date : 2022-04-01 Epub Date: 2020-12-22 DOI: 10.1080/10799893.2020.1859534

Guangwei Zeng, Huixian An, Dong Fang, Wei Wang, Yang Han, Cheng Lian

{"title":"Plantamajoside protects H9c2 cells against hypoxia/reoxygenation-induced injury through regulating the akt/Nrf2/HO-1 and NF-κB signaling pathways.","authors":"Guangwei Zeng, Huixian An, Dong Fang, Wei Wang, Yang Han, Cheng Lian","doi":"10.1080/10799893.2020.1859534","DOIUrl":"https://doi.org/10.1080/10799893.2020.1859534","url":null,"abstract":"Myocardial ischemia/reperfusion (I/R) injury has been found to be associated with oxidative stress. Plantamajoside (PMS) is a major compound of Plantago asiatica that was reported to possess cardioprotective and antioxidant effects. The current study was designed to investigate the effect of PMS on myocardial I/R injury. Rat cardiomyocytes H9c2 cells were exposed to hypoxia/reoxygenation (H/R) to establish in vitro model of myocardial I/R injury. MTT assay proved that H9c2 cells viability was significant reduced under H/R treatment, while the reduction was ameliorated by PMS. H/R-induced ROS production in H9c2 cells was suppressed by PMS. The decreased activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in the H/R group were effectively elevated by PMS. In addition, treatment with PMS attenuated H/R-stimulated production of TNF-α, IL-6 and IL-1β in H9c2 cells. Besides, PMS significantly suppressed bax expression and caspase 3 activity, as well as increased bcl-2 expression in H/R-stimulated H9c2 cells. Furthermore, we also found that PMS significantly enhanced the activation of Akt/Nrf2/HO-1 signaling pathway and suppressed the activation of NF-κB signaling pathway in H/R-stimulated H9c2 cells. These results provided substantial evidence that PMS protected against myocardial I/R injury via attenuating oxidative stress, inflammatory response and apoptosis. The protective effects of PMS were attributed to the Akt/Nrf2/HO-1 and NF-κB signaling pathways.","PeriodicalId":16962,"journal":{"name":"Journal of Receptors and Signal Transduction","volume":"42 2","pages":"125-132"},"PeriodicalIF":2.8,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10799893.2020.1859534","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38739873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Polygonatum sibiricum polysaccharide inhibits high glucose-induced oxidative stress, inflammatory response, and apoptosis in RPE cells. 黄精多糖抑制高糖诱导的RPE细胞氧化应激、炎症反应和凋亡。

IF 2.8 4区生物学

Journal of Receptors and Signal Transduction Pub Date : 2022-04-01 Epub Date: 2021-02-08 DOI: 10.1080/10799893.2021.1883061

Wenjun Wang, Shang Li, Meixia Song

{"title":"Polygonatum sibiricum polysaccharide inhibits high glucose-induced oxidative stress, inflammatory response, and apoptosis in RPE cells.","authors":"Wenjun Wang, Shang Li, Meixia Song","doi":"10.1080/10799893.2021.1883061","DOIUrl":"https://doi.org/10.1080/10799893.2021.1883061","url":null,"abstract":"Diabetic retinopathy is one of the major diabetic complications and remains the most common cause of adult blindness among patients with diabetes mellitus. Polygonatum sibiricum polysaccharides (PSP) are a group important component of Polygonatum sibiricum (PS) with anti-diabetic activity. However, the effect and underlying mechanism of PSP on diabetic retinopathy remains unclear. We used high glucose (HG)-stimulated ARPE-19 cells to establish in vitro diabetic retinopathy model. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay was performed to evaluate cell viability of ARPE-19 cells. The changes in the ROS production, malondialdehyde (MDA) content, and activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) were detected to indicate oxidative stress. The secretion levels of tumor necrosis factor-alpha (TNF-α) and interleukin-8 (IL-8) were detected by ELISA. The protein levels of TNF-α, IL-8, bcl-2, bax, nuclear Nrf2, and anti-hemeoxygenase-1 (HO-1) were detected by western blot analysis. Our results showed that HG treatment caused a significant reduction in cell viability of ARPE-19 cells. PSP treatment improved the reduced cell viability of ARPE-19 cells. PSP also attenuated HG-induced oxidative stress with decreased ROS production and MDA content, as well as increased the activities of SOD and GPx. In addition, HG significantly increased bax expression and caspase-3 activity, and decreased bcl-2 expression. However, these changes were mitigated by PSP treatment. Furthermore, PSP markedly induced the activation of Nrf2/HO-1 pathway in HG-induced ARPE-19 cells. Knockdown of Nrf2 reversed the protective effects of PSP on HG-induced ARPE-19 cells. Taken together, these findings indicated that PSP protects ARPE-19 cells from HG-induced oxidative stress, inflammation, and cell apoptosis through regulation of Nrf2/HO-1 signaling pathway.","PeriodicalId":16962,"journal":{"name":"Journal of Receptors and Signal Transduction","volume":"42 2","pages":"189-196"},"PeriodicalIF":2.8,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/10799893.2021.1883061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25345583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14