Journal of Peptide Science最新文献_第2页

Discovery of Antioxidant Peptide Against Keap1 From Silkworm Protein Based on In Silico Study and In Vitro Antioxidant Activity Detection 家蚕蛋白抗Keap1抗氧化肽的硅研究及体外抗氧化活性检测

IF 1.8 4区生物学

Journal of Peptide Science Pub Date : 2025-08-16 DOI: 10.1002/psc.70049

Shuoliang Ye, Mengyue Qi, Jianhua Wang, Qiuyi Zhang, Lan Fang, Yan Huo, Zhiyong Li

{"title":"Discovery of Antioxidant Peptide Against Keap1 From Silkworm Protein Based on In Silico Study and In Vitro Antioxidant Activity Detection","authors":"Shuoliang Ye, Mengyue Qi, Jianhua Wang, Qiuyi Zhang, Lan Fang, Yan Huo, Zhiyong Li","doi":"10.1002/psc.70049","DOIUrl":"https://doi.org/10.1002/psc.70049","url":null,"abstract":"<div>\u0000 \u0000 <p>Nrf2-Keap1 is an important defense system against oxidative stress damage and enhances the body's antioxidant capacity. Targeting the Keap1-Nrf2 signaling pathway and activating Nrf2 has become an effective strategy for treating oxidative stress and related diseases. In this study, virtual digestion, ADMET prediction, and molecular docking were used to screen antioxidant peptide from <i>silkworm pupa</i> protein. Then, a novel tripeptide (WQK) was identified, exhibiting good water solubility, nontoxicity, high intestinal absorption, and the ability to cross the blood–brain barrier. Molecular docking showed that WQK established six H-bond interactions with some key sites of Keap1 (Arg380, Arg415, Gln530, Ser555, Ile416, and Leu365), which is similar to the identified antioxidant molecule 12e. Additionally, WQK has the function to reduce ABTS·<sup>+</sup> and ferric-tripyridyltriazine (Fe<sup>3+</sup>-TPTZ) in vitro. Furthermore, WQK can promote the expression of antioxidant genes and eliminate reactive oxygen species (ROS) in oxalic acid-treated human proximal tubular epithelial cells (HK-2). The results suggested that WQK may activate the Keap1-Nrf2 signaling pathway by binding to Keap1 and activating the antioxidant system.</p>\u0000 </div>","PeriodicalId":16946,"journal":{"name":"Journal of Peptide Science","volume":"31 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144853699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silica-Assisted Solid-Phase Peptide Synthesis (SiPPS) 硅辅助固相肽合成（SiPPS）

IF 1.8 4区生物学

Journal of Peptide Science Pub Date : 2025-08-16 DOI: 10.1002/psc.70052

Nonsikelelo Nkwanyana, Ashish Kumar, Amit Chakraborty, Steeves Potvin, Georges Thibaut-Koumba, Brunello Nardone, Francois Beland, Anamika Sharma, Beatriz G. de la Torre, Fernando Albericio

引用次数: 0

Interactions of Peptide Amphiphiles With Viruses and Cells Are Enabled by Amorphous Nanostructures 无定形纳米结构使肽两亲体与病毒和细胞的相互作用成为可能

IF 1.8 4区生物学

Journal of Peptide Science Pub Date : 2025-08-15 DOI: 10.1002/psc.70051

Julia La Roche, Lena Rauch-Wirth, Laura Zimmerman, Fabian Zech, Jan Münch, Clarissa Read, Kübra Kaygisiz

引用次数: 0

Assessment of Thermal and Photolytic Stress Effects on the Stability of Primary Structure of Synthetic Liraglutide Using LC-HRMS/MS 热、光解胁迫对合成利拉鲁肽一级结构稳定性的影响

IF 1.8 4区生物学

Journal of Peptide Science Pub Date : 2025-08-15 DOI: 10.1002/psc.70050

Devendra Badgujar, Sanket Bawake, V. K. Yuvaraaj, Dilip Ghava, Nitish Sharma

{"title":"Assessment of Thermal and Photolytic Stress Effects on the Stability of Primary Structure of Synthetic Liraglutide Using LC-HRMS/MS","authors":"Devendra Badgujar, Sanket Bawake, V. K. Yuvaraaj, Dilip Ghava, Nitish Sharma","doi":"10.1002/psc.70050","DOIUrl":"https://doi.org/10.1002/psc.70050","url":null,"abstract":"<div>\u0000 \u0000 <p>Liraglutide (LGT), a synthetic glucagon-like peptide-1 (GLP-1) analogue, is widely used in the treatment of Type 2 diabetes and obesity. Due to its peptide-based nature, it is prone to degradation, particularly under improper storage or handling conditions. Environmental stressors, such as heat and light, can lead to the formation of impurities that may compromise the quality and safety of the drug. In this study, we have investigated the impact of thermal and photolytic stress on LGT stability using reverse-phase liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS). This method enabled the separation, identification, and characterization of intact LGT and its degradation products (DPs). Ten impurities were identified, including four DPs that have not been reported so far. Further, MS/MS fragmentation analysis was employed to elucidate the sequences of LGT and its impurities, pinpointing the modifications and their respective sites. The formation mechanisms of these impurities were also proposed, providing critical insights into the degradation pathways. This study offers a comprehensive analytical approach for assessing the stability of peptide drugs, contributing to enhanced quality control and safer pharmaceutical formulations.</p>\u0000 </div>","PeriodicalId":16946,"journal":{"name":"Journal of Peptide Science","volume":"31 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144843397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulation of Antimicrobial Activities of Aib-Based Artificial Amphipathic α-Helical Peptides by Incorporating Histidine Residues 组氨酸残基对aib型人工两性α-螺旋肽抗菌活性的调节作用

IF 1.8 4区生物学

Journal of Peptide Science Pub Date : 2025-07-29 DOI: 10.1002/psc.70046

Ami Koga, Manami Fuchinoue, Kiyohiko Seki, Kaoru Araki, Tomoichirou Kusumoto, Junichi Taira, Hiroaki Kodama, Satoshi Osada

{"title":"Modulation of Antimicrobial Activities of Aib-Based Artificial Amphipathic α-Helical Peptides by Incorporating Histidine Residues","authors":"Ami Koga, Manami Fuchinoue, Kiyohiko Seki, Kaoru Araki, Tomoichirou Kusumoto, Junichi Taira, Hiroaki Kodama, Satoshi Osada","doi":"10.1002/psc.70046","DOIUrl":"https://doi.org/10.1002/psc.70046","url":null,"abstract":"<div>\u0000 \u0000 <p>Cationic antimicrobial peptides (CAMPs) exhibit potent antibacterial activity by disrupting bacterial membranes. We investigated the effect of histidine incorporation on BKBA-20, a designed amphiphilic helical peptide composed of alternating 2-aminoisobutyric acid (Aib) and lysine. Substitution at lysine sites (<b>1a</b>–<b>1e</b> series) reduced net charge and antimicrobial activity, though certain analogues (<b>1c</b>, <b>1d</b>) demonstrated minimal antibacterial activity against <i>Escherichia coli</i>. In contrast, substitution at Aib sites (<b>2a</b>–<b>2c</b> series) preserved some extent of helical structure and improved activity under acidic conditions. Notably, substitutions at the terminal of the peptide were more effective at acidic pH, while the slightly medial side of the peptide favored activity at neutral pH. Hemolysis assays confirmed low cytotoxicity of the modified peptides. These results suggest histidine incorporation as a promising strategy to broaden the spectrum of CAMPs, particularly against Gram-negative bacteria, without increasing toxicity.</p>\u0000 </div>","PeriodicalId":16946,"journal":{"name":"Journal of Peptide Science","volume":"31 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144725748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controversial Nomenclature in Peptide Synthesis: A Call for Clarity 肽合成中有争议的命名法：呼吁澄清

IF 1.8 4区生物学

Journal of Peptide Science Pub Date : 2025-07-27 DOI: 10.1002/psc.70044

Anamika Sharma, Ashish Kumar, Beatriz G. de la Torre, Fernando Albericio

引用次数: 0

Peptide Synthesis Nomenclature 肽合成术语

IF 1.8 4区生物学

Journal of Peptide Science Pub Date : 2025-07-27 DOI: 10.1002/psc.70043

Christian F. W. Becker, Florine Cavelier, Yosuke Demizu, Xuechen Li, William Lubell, John Wade, Paolo Rovero

引用次数: 0

Therapeutic Potential of Stylissatin A and Related Cyclic Peptides From Marine Sponges 海海绵中Stylissatin A及相关环肽的治疗潜力

IF 1.8 4区生物学

Journal of Peptide Science Pub Date : 2025-07-24 DOI: 10.1002/psc.70045

Aaqib Ullah, Farzana Shaheen, Uzma Salar, Andreas G. Tzakos, Ioannis P. Gerothanassis

{"title":"Therapeutic Potential of Stylissatin A and Related Cyclic Peptides From Marine Sponges","authors":"Aaqib Ullah, Farzana Shaheen, Uzma Salar, Andreas G. Tzakos, Ioannis P. Gerothanassis","doi":"10.1002/psc.70045","DOIUrl":"https://doi.org/10.1002/psc.70045","url":null,"abstract":"<p>Marine sponges are sessile invertebrates found in moderate, arctic, and tropical regions, serving as a valuable reservoir of bioactive compounds, particularly Pro-rich peptides. Among these, cyclic peptides have attracted significant interest due to their diverse therapeutic properties. One notable example is Stylissatin A (SA), a Pro-rich cyclic peptide reported from the marine sponge <i>Stylissa massa</i>. SA and its analogues have shown promising biological activities, including anti-inflammatory, anticancer, and anti-obesity effects. Despite the vast potential of marine-derived peptides, only a small number have progressed to the pharmaceutical market. Cyclic peptides like SA offer unique opportunities for molecular modifications and total synthesis, enabling the enhancement of potency, improvement of physicochemical properties, and optimization of synthetic yields. This review highlights the synthetic strategies developed for the total synthesis of SA, explores its structural features and related analogues, and discusses their therapeutic potential, underscoring the promise of SA-based scaffolds as novel peptide-based drug candidates.</p>","PeriodicalId":16946,"journal":{"name":"Journal of Peptide Science","volume":"31 9","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/psc.70045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144687876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical Synthesis of Medin via a Removable Aggregation-Suppressing Linker 用可移动抑制聚集连接剂化学合成麦丁

IF 1.8 4区生物学

Journal of Peptide Science Pub Date : 2025-07-13 DOI: 10.1002/psc.70041

Matthias De Vleeschouwer, Brajabandhu Pradhan, Frederic Rousseau, Joost Schymkowitz

{"title":"Chemical Synthesis of Medin via a Removable Aggregation-Suppressing Linker","authors":"Matthias De Vleeschouwer, Brajabandhu Pradhan, Frederic Rousseau, Joost Schymkowitz","doi":"10.1002/psc.70041","DOIUrl":"https://doi.org/10.1002/psc.70041","url":null,"abstract":"<div>\u0000 \u0000 <p>Medin, a 50-amino acid fragment derived from the protein MFG-E8 (lactadherin), is the most prevalent amyloid found in humans, present in the vasculature of nearly all individuals over the age of 50. Its biological relevance is highlighted by its co-localization with amyloid-β (Aβ) deposits in both Alzheimer's disease patients and transgenic mice models. Notably, Medin promotes amyloid-β aggregation, forming mixed fibrils with Aβ and enhancing its deposition in blood vessels. Here we report a new and efficient strategy to chemically access this compound. Our approach employs a solubilizing linker that not only ensures high solubility but also suppresses aggregation, allowing efficient purification of the product. The linker can be removed without a trace, after which the product behaves identically to wild-type Medin and forms amyloid fibrils. The synthesis route allows opening up a new chemical space, including nonnatural modifications like biotinylation. Together with the control over the aggregation properties, this is a powerful tool for amyloid protein studies.</p>\u0000 </div>","PeriodicalId":16946,"journal":{"name":"Journal of Peptide Science","volume":"31 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptide-Drug Conjugates: A New Hope for Cancer 肽-药物偶联物：癌症的新希望

IF 1.8 4区生物学

Journal of Peptide Science Pub Date : 2025-07-11 DOI: 10.1002/psc.70040

Amy Armstrong, Fleur Coburn, Yanyamba Nsereko, Othman Al Musaimi

{"title":"Peptide-Drug Conjugates: A New Hope for Cancer","authors":"Amy Armstrong, Fleur Coburn, Yanyamba Nsereko, Othman Al Musaimi","doi":"10.1002/psc.70040","DOIUrl":"https://doi.org/10.1002/psc.70040","url":null,"abstract":"<p>Peptide-drug conjugates (PDCs) are advancing as targeted cancer therapies, leveraging lessons from antibody-drug conjugates (ADCs) to improve tumour specificity. These molecules combine a homing peptide with a cytotoxic payload via a linker, enabling precise drug delivery while sparing healthy tissue. Despite their potential, PDCs face challenges including metabolic instability, premature payload release and rapid clearance, limiting clinical success. Only Lutathera remains FDA-approved after Pepaxto's withdrawal, though Pepaxto retains EMA and MHRA approval—highlighting regulatory and technical complexities. Most PDCs target overexpressed receptors (e.g., somatostatin and GnRH), though novel designs like CBX-12 employ alternative strategies. Currently, six PDCs are in Phase III trials, with ~96 in development, signalling growing interest. This review explores how ADC research has guided PDC optimisation, particularly in linker chemistry and payload selection. We analyse key structural features governing PDC efficacy, including peptide-receptor binding and intracellular trafficking. Innovations in stable linkers and tumour-selective activation mechanisms are critical to overcoming pharmacokinetic hurdles. Promising candidates in late-stage trials are highlighted, emphasising their potential to address unmet needs in oncology. By refining targeting precision and payload delivery, next-generation PDCs may expand treatment options for resistant cancers, bridging the gap between biologics and small-molecule therapies.</p>","PeriodicalId":16946,"journal":{"name":"Journal of Peptide Science","volume":"31 8","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/psc.70040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144606656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0