Russian Journal of Biological Physics and Chemisrty最新文献_第9页

MITOCHONDRIA-TARGETED ANTIOXIDANT SKQ1 REGULATES OF SIGNAL SYSTEM KEAP1/NRF2/ARE AND APOPTOSIS IN THE LEUKOCYTES OF BLOOD UNDER OXIDATIVE STRESS 线粒体靶向抗氧化剂skq1调控氧化应激下血液白细胞的信号系统keap1 / nrf2 / are和凋亡

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-24 DOI: 10.29039/rusjbpc.2022.0569

O. Gutsenko, I. Kornienko, A. Ananyan, N. Milutina, V. Vnukov

{"title":"MITOCHONDRIA-TARGETED ANTIOXIDANT SKQ1 REGULATES OF SIGNAL SYSTEM KEAP1/NRF2/ARE AND APOPTOSIS IN THE LEUKOCYTES OF BLOOD UNDER OXIDATIVE STRESS","authors":"O. Gutsenko, I. Kornienko, A. Ananyan, N. Milutina, V. Vnukov","doi":"10.29039/rusjbpc.2022.0569","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0569","url":null,"abstract":"This study demonstrated that hyperoxia induced (0.5 MPa, 90 min) oxidative stress decreased mRNA level of Nrf2 transcription factor and Nrf2 induced genes encoding antioxidant enzymes (SOD1, CAT, GPx4) in leukocytes of rat blood. The change in gene expression profiles in hyperoxia was accompanied by disbalance of antioxidant enzyme activities in the leukocytes – activation of superoxide dismutase, and inhibition of catalase, glutathione peroxidase. The administration of SkQ1 (50 nmol/kg during 5 days) significantly increased mRNA level of Nrf2 transcription factor and Nrf2 induced genes encoding antioxidant enzymes SOD2 and GPx4 and nor malized the transcriptional activity of the SOD1 and CAT genes in the leukocytes under the hyperoxia induced oxidative stress. Catalase and glutathione peroxidase activity in the leukocytes increased concurrently with reversion of superoxide dismutase activity to the control level. The protective effect of SkQ1 in hyperoxia induced oxidative stress may be realized via direct antioxidant activity and the stimulation of the Keap1/Nrf2/ARE defense system. Administration of SkQ1 in extremely low amounts (50 nmol/kg for 5 days) effectively inhibits the accumulation of lipid peroxidation molecular products, normalizes the structural state of lymphocyte membranes and the level of their apoptosis under the condition of physiological standard and oxidative stress.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124008349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LYMPHOCYTES APOPTOSIS UNDER THE ACTION OF PHYSICAL FACTORS COMBINATION 物理因素联合作用下淋巴细胞凋亡

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-15 DOI: 10.29039/rusjbpc.2022.0524

E. Nikanorova, I. Varganova, Y. Medvedev, V. Nagiba

{"title":"LYMPHOCYTES APOPTOSIS UNDER THE ACTION OF PHYSICAL FACTORS COMBINATION","authors":"E. Nikanorova, I. Varganova, Y. Medvedev, V. Nagiba","doi":"10.29039/rusjbpc.2022.0524","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0524","url":null,"abstract":"The article presents the comparative study results of the bioeffects of 30-minute exposure session to electromagnetic radiation with 1000 MHz frequency and 10 mW/cm2 EFD; of 30-minute exposure session to low-frequency pulsed magnetic field with 4 mT magnetic induction, as well as their combination, on rat’s lymphocytes apoptosis level in vivo. The apoptosis level was assessed morphologically with fluorescent microscope after 24-hours of cell suspension cultivation at immunological plates at 37 0C in nutrient medium without loading. The percentage of cells with morphological signs of apoptosis (margination, condensation and fragmentation of chromatin in nucleus) was quantitatively determined. It was found that electromagnetic irradiation had no significant impact on apoptosis level, but magnetic field action resulted in reducing the apoptosis cells number by 16% (p≤0,01) compared to the control group. The combination of electromagnetic radiation and low intensity magnetic field increased the apoptotic cells number by 28% (p ≤0.01) compared to the control. Thus, the mediated effect of electromagnetic radiation on blood cells genome leads to disruption of its normal functioning. It has been shown that low-frequency magnetic field can influence on intracellular processes in different directions; in combination with electromagnetic radiation it can intensify pathological reactions, and thus launching the apoptosis massive program.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134178029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PROSPECTS FOR USING DELAYED FLUORESCENCE OF ALGAE FOR CONTAMINATION BIOTESTING 藻类延迟荧光在污染生物检测中的应用前景

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-15 DOI: 10.29039/rusjbpc.2022.0525

D. Matorin, O. Yakovleva, D. Todorenko, S. Goryachev, A. Alekseev

引用次数: 0

EFFECT OF COBALT FERRITE MAGNETIC NANOPARTICLES IN HYDROPHILIC COATING ON THE CONDUCTIVITY OF BILAYER LIPID MEMBRANES 亲水涂层中钴铁氧体磁性纳米颗粒对双层脂膜电导率的影响

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-15 DOI: 10.29039/rusjbpc.2022.0501

E. Borisova, B. Kozhemova, O. Konstantinov, E. Korepanova, V. Mikheev, A. Mikhnich, V. Sukhova, P. Astanina, O. Koplak, A. Anosov

{"title":"EFFECT OF COBALT FERRITE MAGNETIC NANOPARTICLES IN HYDROPHILIC COATING ON THE CONDUCTIVITY OF BILAYER LIPID MEMBRANES","authors":"E. Borisova, B. Kozhemova, O. Konstantinov, E. Korepanova, V. Mikheev, A. Mikhnich, V. Sukhova, P. Astanina, O. Koplak, A. Anosov","doi":"10.29039/rusjbpc.2022.0501","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0501","url":null,"abstract":"Magnetic nanoparticles are widely used in medical applications, for example, as contrast agents for magnetic resonance imaging, to enhance hyperthermia, for targeted drug delivery, theranostics (therapy + diagnostics). When used in vivo, magnetic nanoparticles require a special coating that promotes biocompatibility and minimizes potential toxicity under physiological conditions. Human serum albumin or polyethylene glycol can be used as a coating. In this work, the interactions of magnetic cobalt ferrite nanoparticles (12 and 27 nm size) with diphitanoylphosphatidylcholine bilayer lipid membranes were investigated. The conductivity of membranes varied over a wide range – two groups of membranes can be distinguished – membranes, the conductivity of which (after the addition of nanoparticles) remained practically unchanged compared to the control, and membranes, the conductivity of which, upon the addition of nanoparticles, changed abruptly and increased in a wide range by 0.5–2 orders of magnitude. Significant differences between the conductivities upon the addition of the same volume of 12 and 27 nm nanoparticles surrounded by human serum albumin were not found. When adding nanoparticles with a coating of polyethylene glycol, the conductivity increased much weaker than when adding nanoparticles with a coating of human serum albumin. The results suggest that hydrophilic coated magnetic nanoparticles interact with the membranes, which can lead to the appearance of metastable conducting pores, which, in turn, increase the integral conductivity of the membranes.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"59 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120961433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DIMERIZATION OF ALUMINUM PHTHALOCYANINE CHLORIDE IN ORGANIC AND AQUA-ORGANIC MEDIUM 氯酞菁铝在有机和水-有机介质中的二聚化

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-15 DOI: 10.29039/rusjbpc.2022.0507

I. Klimenko, T. Astakhova, E. Timokhina, A. Lobanov

{"title":"DIMERIZATION OF ALUMINUM PHTHALOCYANINE CHLORIDE IN ORGANIC AND AQUA-ORGANIC MEDIUM","authors":"I. Klimenko, T. Astakhova, E. Timokhina, A. Lobanov","doi":"10.29039/rusjbpc.2022.0507","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0507","url":null,"abstract":"Aluminum phthalocyanine chloride (AlClPc) is a photoactive macroheterocyclic compound, which, in its monomeric form, is used as a photosensitizer (PS) in photodynamic therapy and diagnostics. In this paper, its physicochemical properties were studied in organic (N, N-dimethylformamide, DMF) and aqua-organic (DMF-aqua) media. It has been shown that the hydrophobic properties of AlClPc prevent its widespread use in various pharmacological compositions due to its tendency to aggregate in aqueous solutions, which leads to the formation of non-fluorescent aggregates and a decrease in its photodynamic activity. The geometry and electronic structure of AlClPc in the monomeric and dimerized (H- and \u0000J-aggregates) states were studied using quantum-chemical calculations with the help of the electron density functional theory (DFT) method. Different types of orientation during the dimerization of AlClPc molecules are presented: “back to back”, “head to head”, “head to back”, as well as mixed-oriented types. It has been proven that both in DMF and DMF-aqua media, the preferred orientation is “back to back”, without sacrificing the monomeric geometry of the constituent molecules in the dimer. It is shown that in an aqueous organic solvent the AlClPc molecule is easily hydrated with the formation of a coordination bond between the Al atom of the AlClPc molecule and the O atom of the aqua molecule. The bond length is 2.23 Å, and the hydration energy is 16.84 kcal/mol. Hydration promotes the formation of dimers, in which two aqua molecules play the role of \"bridges\" between two AlClPc molecules. In such dimer, each aqua molecule has two bonds: one coordination bond between its O atom and the Al atom of one of the AlClPc molecules and one hydrogen bond between its H atom and the N atom of another AlClPc molecule. Based on the calculated data obtained, the AlClPc dimers in the DMF medium were assigned to H-aggregates, and in the DMF-water medium, to J-aggregates, respectively.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126690093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE EFFECTS OF COBRA CARDIOTOXINS ON THE PAPILLARY MUSCLE AND LANGENDORF PERFUSED RAT HEART ARE NOT ASSOCIATED WITH ADRENALINE RELEASE 眼镜蛇心脏毒素对大鼠乳头肌和兰根多夫灌注心脏的影响与肾上腺素释放无关

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-15 DOI: 10.29039/rusjbpc.2022.0515

A. Averin, F. Tyurin, K. Samodurova, V. Starkov, T. Andreeva, Y. Utkin

{"title":"THE EFFECTS OF COBRA CARDIOTOXINS ON THE PAPILLARY MUSCLE AND LANGENDORF PERFUSED RAT HEART ARE NOT ASSOCIATED WITH ADRENALINE RELEASE","authors":"A. Averin, F. Tyurin, K. Samodurova, V. Starkov, T. Andreeva, Y. Utkin","doi":"10.29039/rusjbpc.2022.0515","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0515","url":null,"abstract":"In a study of the effects of cobra cardiotoxins on myocardial tissue, both right ventricular (papillary muscle) and left ventricular contractility (isovolumic recording of left ventricular pulse pressure during Langendorff perfusion of the heart) were assessed. In papillary muscle, both toxins, at a concentration of 5 μg/mL, caused short-term increases in contractility to 200±25% and 171±15% for CTX-1 and CTX-2, respectively, at the point of maximum effect. At the same time, for CTX-1 and CTX-2, the time to peak tension (TPT) increased from 104±2 to 111±2 and from 96±2 to 104±5 ms, the relaxation time to 50%(TR50%) from 64±4 to 70±6 and from 64±6 to 69±7 ms, and the relaxation time to 95%(TR95%) from 163±10 to 190±22 and from 148±16 to 155±20 ms, respectively. This significantly differs from the positive inotropic effect of the β-adrenomimetic isoproterenol (170±31%), which causes acceleration of TPT from 106±5 to 89±4 ms, TR50% from 58±6 to 43±4 ms, and TR95% from 145±15 to 90±14 ms. When the whole heart was exposed to cardiotoxins, an increase in contractility was also observed, followed by its suppression and contracture, in contrast to isoproterenol, which caused a steady increase in contractility coupled with an increase in heart rate. Pretreatment of papillary muscles with the β-blocker propranolol \u0000(10 μM) did not prevent the development of cardiotoxin effects, but completely blocked the effects of isoproterenol. Our data indicate that the temporary increase in contractility under the action of cardiotoxins is not associated with the release of endogenous adrenaline, but rather is caused by changes in calcium homeostasis in cardiomyocytes.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134405780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRODUCTION OF REACTIVE OXYGEN SPECIES UNDER THE ACTION OF UV-B RADIATION ON YEAST CELLS 酵母细胞在uv-b辐射作用下活性氧的产生

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-15 DOI: 10.29039/rusjbpc.2022.0503

N. Kocharli, S. Hummatova

{"title":"PRODUCTION OF REACTIVE OXYGEN SPECIES UNDER THE ACTION OF UV-B RADIATION ON YEAST CELLS","authors":"N. Kocharli, S. Hummatova","doi":"10.29039/rusjbpc.2022.0503","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0503","url":null,"abstract":"The present investigation is devoted to the study of the effect of ultraviolet-B (UV-B) radiation on the survival and production of reactive oxygen species in yeast cells. It has been determined that under the action of UV-B radiation on cells, depending on its dose, the rate of oxidation of the dye 2',7'-dichlorodihydrofluorescein diacetate (H2DCF•DA) increases and a high intensity of DCF fluorescence is observed. When cells are modified with ascorbic acid before irradiation, the rate of H2DCF oxidation and the intensity of DCF fluorescence decreases. The survival rate of modified cells is increased. Ascorbic acid reduces the amount of ROS in the suspension of irradiated cells. At high doses (4.5-10 erg/mm2) of irradiation, the antioxidant effect of ascorbic acid in yeast cells is negligible. The concentration of 2-4 dinitrophenol (DNF) 10-7 M was determined as a protector, in which the substance potentially contributes to the manifestation of the effect of \"soft uncoupling\" in cells and, under the influence of UV-B radiation on yeast cells, helps to reduce the production of reactive oxygen species and an increase in survival. High concentrations (10–3M–10–5 M) of DNP adversely affect the survival of yeast cells and ROS production. So, it can be concluded that under the action of UV-B rays on yeast cells, antioxidants can possibly participate in the regulation of redox homeostasis.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"212 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114670123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EVALUATION OF THE PIGMENT COMPOSITION OF MICROALGAE PORPHYRIDIUM PURPUREUM ACCORDING TO THE TRUE ABSORPTION SPECTRUM OF THE CULTURE USING A SPREADSHEET PROCESSOR 利用电子表格处理器根据培养物的真实吸收光谱评价微藻紫卟啉的色素组成

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-15 DOI: 10.29039/rusjbpc.2022.0504

D. Chernyshev, V. Klochkova, E. Seryak

{"title":"EVALUATION OF THE PIGMENT COMPOSITION OF MICROALGAE PORPHYRIDIUM PURPUREUM ACCORDING TO THE TRUE ABSORPTION SPECTRUM OF THE CULTURE USING A SPREADSHEET PROCESSOR","authors":"D. Chernyshev, V. Klochkova, E. Seryak","doi":"10.29039/rusjbpc.2022.0504","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0504","url":null,"abstract":"The paper describes a method for mathematical separation of the absorption spectrum of the culture of the red seaweed Porphyridium purpureum using a spreadsheet processor - the Microsoft Excel program. This method represents the analysis and separation of overlapping pigment bands in the native absorption spectrum of a culture. The culture spectra were recorded on a spectrophotometer near and at a distance from the entrance window of the integrating sphere. The true absorption spectrum is calculated, compensated for scattering in the region from 400 to 750 nm. The true absorption spectrum of the culture was approximated by a mathematical model in which the unknown parameters are the concentrations of chlorophyll a, B-phycoerythrin, R-phycocyanin, allophycocyanin, and total carotenoids. Pigment models are represented by the sum of normal distribution curves. The implementation of calculations that perform the selection of pigment concentrations by minimizing the sum of squared deviations between the true absorption spectrum and its mathematical model was performed using a spreadsheet using the \"Search for a solution\" tool. The proposed method of mathematical processing of the spectrum can be used as an express method for determining the concentration of pigments in culture, without isolating pure pigments. The application of this method makes it possible to evaluate the contribution of pigments to the total absorption spectrum of the culture.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125355310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LIPOSOMAL FORM WITH LIPOIC ACID AND CARNOSINE: PRODUCTION, ANTIPLATELET AND ANTIOXIDANT EFFECT 脂质体形式具有硫辛酸和肌肽的产生、抗血小板和抗氧化作用

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-15 DOI: 10.29039/rusjbpc.2022.0519

V. Shchelkonogov, O. Baranova, A. Chekanov, K. Kazarinov, N. Shastina, S. Stvolinsky, T. Fedorova, E. Solovieva, A. Fedin, G. Sorokoumova

{"title":"LIPOSOMAL FORM WITH LIPOIC ACID AND CARNOSINE: PRODUCTION, ANTIPLATELET AND ANTIOXIDANT EFFECT","authors":"V. Shchelkonogov, O. Baranova, A. Chekanov, K. Kazarinov, N. Shastina, S. Stvolinsky, T. Fedorova, E. Solovieva, A. Fedin, G. Sorokoumova","doi":"10.29039/rusjbpc.2022.0519","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0519","url":null,"abstract":"Optimal conditions were selected for obtaining the liposomal form with lipoic acid (LA) and carnosine. Using methods of active and passive loading, it was possible to achieve high efficiency inclusion of carnosine (60±5%) and lipoic acid (75±5%) in nanoparticles (NPs). It has been shown that the addition of sucrose or cholesterol (Chol) to nanodispersions (NDs) led to a slight decrease in the efficiency incorporation of carnosine in nanoparticles (45±5%). The production of PC-liposomes (PC-Ls) with LA and carnosine by passive loading led to a significant decrease in the efficiency inclusion of carnosine in liposomes (16%). At the same time, the efficiency of inclusion of LA in PC liposomes using methods of both passive and active loading practically did not change (58-69%). It has been found that the obtained nanodispersions are homogeneous system of nanoparticles with size of 175-250 nm. By transmission electron microscopy, it has been shown that LA+Carn. Liposomes represent a homogeneous system consisting mainly of spherical nanoparticles with a size of 120-200 nm. It is important to note that the obtained liposomes with LA and carnosine are stable during long-term storage (15 months) at + 4 ° and at room temperature. It was established that liposomes LA with Carn exhibit an antioxidant effect, leading to a 15-fold decrease in the concentration of lipid peroxidation products. The effect of the obtained liposomal forms on platelet aggregation caused by arachidonic acid has been revealed. It has been found, that liposomes with LA and carnosine reduce the degree of aggregation of platelets by 60-70%, relative to controls.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131349295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EFFECT OF Cr2(SO4)3 ON A STATUS OF CHLORELLA VULGARIS CULRURE CELLS UNDER THE DIFFERENT CONTENT OF NITROGEN SOURSE – KNO3 IN THE NUTRIENT MEDIUM 营养培养基中不同氮源KNO3含量下Cr2(SO4)3对小球藻培养细胞状态的影响

Russian Journal of Biological Physics and Chemisrty Pub Date : 2022-11-15 DOI: 10.29039/rusjbpc.2022.0526

I. Ilyuchyk, L. Zakharevich, V. Nikandrov

{"title":"EFFECT OF Cr2(SO4)3 ON A STATUS OF CHLORELLA VULGARIS CULRURE CELLS UNDER THE DIFFERENT CONTENT OF NITROGEN SOURSE – KNO3 IN THE NUTRIENT MEDIUM","authors":"I. Ilyuchyk, L. Zakharevich, V. Nikandrov","doi":"10.29039/rusjbpc.2022.0526","DOIUrl":"https://doi.org/10.29039/rusjbpc.2022.0526","url":null,"abstract":"The Cr2(SO4)3 effect at 10–2–10–8 M concentration on the biomass yield, the concentration of intracellular protein, chlorophylls a and b, and carotenoids on the culture of Chlorella vulgaris strain C 111 IBCE C-19 was studied. In separate experiment series, the nitrogen source, KNO3 concentration was increased by 10 and 20%. Chromium (III) had an ambiguous effect on the functional and metabolic state of the chlorella culture, depending on its concentration and the nitrogen source – KNO3 concentration. In general, at 5.0 g/l KNO3 concentration, chromium sulfate in the 10–2–10–6 M concentration inhibited the algae culture development, and at the maximum concentration caused rapid death. In the initial period of cultivation, the level of chlorophylls a and b sharply increased, which, apparently, was of a compensatory-adaptive nature. From the side of biomass, the level of intracellular protein, chlorophylls, no dynamics of an oscillatory nature, inherent in the control variant of the nutrient medium, was revealed. An increase in the concentration of KNO3 to 5.5 g/l at Cr2(SO4)310–4–10–8 M concentration was not only accompanied by an increase in biomass, but also by a noticeable (17–74%) increase in the intracellular protein level, a decrease in the “tension” of the photosynthetic apparatus (judgment by the level of pigments) and restoration of the oscillatory dynamics of photosynthetic pigments. A further increase in the potassium nitrate concentration did not give a positive result and led to the aggravation of the chlorella culture functional and metabolic state.","PeriodicalId":169374,"journal":{"name":"Russian Journal of Biological Physics and Chemisrty","volume":"92 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114105653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0