Journal of Raman Spectroscopy最新文献_第10页

Assessment of unmixing approaches for the quantitation of SERS nanoparticles in highly multiplexed spectral images 评估在高度复用光谱图像中量化 SERS 纳米粒子的非混合方法

IF 2.5 3区化学

Journal of Raman Spectroscopy Pub Date : 2024-01-22 DOI: 10.1002/jrs.6653

Alexander Czaja, Samer Awad, Olga E. Eremina, Augusta Fernando, Cristina Zavaleta

{"title":"Assessment of unmixing approaches for the quantitation of SERS nanoparticles in highly multiplexed spectral images","authors":"Alexander Czaja, Samer Awad, Olga E. Eremina, Augusta Fernando, Cristina Zavaleta","doi":"10.1002/jrs.6653","DOIUrl":"10.1002/jrs.6653","url":null,"abstract":"Surface-enhanced Raman scattering nanoparticles (SERS NPs) offer powerful optical contrast features for imaging assays. Their gold core enhances the inelastic scattering cross section, allowing highly sensitive and rapid detection, and their characteristic sets of narrow spectral bands give them unsurpassed multiplexing capabilities. Multiplexed hyperspectral images are commonly unmixed using a compensation matrix of reference spectra to produce quantitative image channels illustrating the distribution of each material. It is these unmixed channels that are fit for interpretation from assays utilizing SERS NP contrast agents. Some factors that may impact SERS NP quantitative and dynamic range capabilities may include endogenous background heterogeneity, the ability of unmixing algorithms to account for signal variances, and linear system conditioning imposed by contrast agent signals. We report on hyperspectral Raman imaging of mixtures of SERS NPs from an expanded library of contrast agents. We study increasing plexity and varying degrees of system conditioning as inputs to a diverse set of classical, non-negatively constrained, and regularized regression algorithms to investigate which signal features and unmixing methods deliver the most promising quantitation performance with the least error. Raman imaging of SERS NP mixtures is performed on controlled substrates and representative biological specimens, and experimental results are compared against ground truth data. We evaluate spectral fitting fidelity, quantitation, and specificity correlations with system conditioning. Spectral unmixing with a regularized hybrid of least squares regression with principal component analysis (HLP) algorithm approximated spectra with 3.5× better fitting fidelity and 3× better quantitation robustness with tissue background compared with simpler unmixing routines.","PeriodicalId":16926,"journal":{"name":"Journal of Raman Spectroscopy","volume":"55 5","pages":"566-580"},"PeriodicalIF":2.5,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139559643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spectroscopic assessment of biomolecular changes in Helicobacter pylori and Epstein–Barr virus co-infected gastric epithelial cells 通过光谱评估幽门螺杆菌和爱泼斯坦-巴氏病毒共同感染的胃上皮细胞的生物分子变化

IF 2.5 3区化学

Journal of Raman Spectroscopy Pub Date : 2024-01-21 DOI: 10.1002/jrs.6652

Dharmendra Kashyap, Manushree Tanwar, Chanchal Rani, Pranit Hemant Bagde, Siddharth Singh, Nidhi Varshney, Vaishali Saini, Amit Mishra, Rajesh Kumar, Hem Chandra Jha

{"title":"Spectroscopic assessment of biomolecular changes in Helicobacter pylori and Epstein–Barr virus co-infected gastric epithelial cells","authors":"Dharmendra Kashyap, Manushree Tanwar, Chanchal Rani, Pranit Hemant Bagde, Siddharth Singh, Nidhi Varshney, Vaishali Saini, Amit Mishra, Rajesh Kumar, Hem Chandra Jha","doi":"10.1002/jrs.6652","DOIUrl":"10.1002/jrs.6652","url":null,"abstract":"Helicobacter pylori and Epstein–Barr Virus (EBV) are Group 1 carcinogens that can enhance gastric cancer progression. Bioactive substances extracted from plants can be effective therapeutic agents in cancer treatment. For example, Withania somnifera extract-WSE reduces the Gankyrin oncoprotein, which is upregulated in the presence of H. pylori and EBV. The various biochemical and metabolic changes upon 24 hrs post-infection followed by W. somnifera extract (WSE) treatment on gastric epithelial cells (AGS) can be studied using spectroscopic techniques. In the biomedical sciences, Raman and NMR spectroscopy have been extensively employed to interpret cellular alterations contributing to the onset of infection and the severity of gastric cancer. More specifically, alterations in cellular biochemical homeostasis are linked to the moieties of cholesterol, collagen, choline, carbohydrate, lipids, tyrosine, and phenylalanine. Further, we have found significantly elevated FWHM for carbohydrates, tumor associated protein, collagen, cholesterol, and cholesterol ester in the co-infection model. We also looked into the potential correlation between these molecules using molecular network analysis and found several related factors that can be modulated through biomolecular levels. These molecules are crucial in several physiological functions, including cell division, cell proliferation, apoptosis, necrosis, cell migration, and lipid transport. Our study paves the pathway to study H. pylori and EBV co-infection in human gastric epithelial cells and the therapeutic interventions of WSE in this scenario and highlights specific biomolecular alterations, which can be focused for further mechanistic investigations.","PeriodicalId":16926,"journal":{"name":"Journal of Raman Spectroscopy","volume":"55 5","pages":"549-565"},"PeriodicalIF":2.5,"publicationDate":"2024-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139516046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

(Resonance) Raman optical activity among chiroptical spectroscopies: Quo vadis? (共振）拉曼光谱中的光电活动：何去何从？

IF 2.5 3区化学

Journal of Raman Spectroscopy Pub Date : 2024-01-18 DOI: 10.1002/jrs.6650

Monika Halat, Katarzyna Pajor, Rafal Baranski, Malgorzata Baranska

{"title":"(Resonance) Raman optical activity among chiroptical spectroscopies: Quo vadis?","authors":"Monika Halat, Katarzyna Pajor, Rafal Baranski, Malgorzata Baranska","doi":"10.1002/jrs.6650","DOIUrl":"10.1002/jrs.6650","url":null,"abstract":"Although the potential of Raman Optical Activity (ROA) spectroscopy in the field of organic and inorganic materials research has been well demonstrated, its practical application is still limited. This work shows historical background, current state of the art and challenges of ROA spectroscopy facing scientists today. Expectations and development prospects for the coming years are also discussed. Efforts have already been made to improve but also to properly interpret the ROA signal when the ROA technique is combined with other spectroscopies (i.e., ROA/Electronic Circular Dichroism and ROA/Circularly Polarized Luminescence). The paper shows how to intensify the ROA effect to shorten the spectrum accumulation time, control possible artefacts and support the analysis of ROA spectra with actual and new theoretical approaches. However, the challenge is to increase the interest in this technique among the academic community, develop reliable protocols and adapt them for practical use. National and international research networks can significantly contribute to the growth of methodologies and standardization in areas in which the ROA has so far been little used. This paper presents a perspective vision for the future progress of ROA alongside other chiroptical methods.","PeriodicalId":16926,"journal":{"name":"Journal of Raman Spectroscopy","volume":"55 5","pages":"539-548"},"PeriodicalIF":2.5,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139496127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Raman spectroscopy of Wadsley phases of vanadium oxide 氧化钒瓦兹利相的拉曼光谱学

IF 2.5 3区化学

Journal of Raman Spectroscopy Pub Date : 2024-01-15 DOI: 10.1002/jrs.6644

Petr Shvets, Alexander Krylov, Ksenia Maksimova, Goikhman

引用次数: 0

Characterization of carfentanil and thiofentanil using surface-enhanced Raman spectroscopy and density functional theory 利用表面增强拉曼光谱和密度泛函理论表征卡芬太尼和硫芬太尼

IF 2.5 3区化学

Journal of Raman Spectroscopy Pub Date : 2024-01-10 DOI: 10.1002/jrs.6643

Rasmus Öberg, Lars Landström, Eduardo Gracia-Espino, Andreas Larsson, Magnus Andersson, Per Ola Andersson

{"title":"Characterization of carfentanil and thiofentanil using surface-enhanced Raman spectroscopy and density functional theory","authors":"Rasmus Öberg, Lars Landström, Eduardo Gracia-Espino, Andreas Larsson, Magnus Andersson, Per Ola Andersson","doi":"10.1002/jrs.6643","DOIUrl":"10.1002/jrs.6643","url":null,"abstract":"Fentanyls are synthetic opioids up to 10,000 times more potent than morphine. Although initially developed for medical applications, fentanyl and its analogues have recently grown synonymous with the ongoing opioid epidemic. To combat the continued spread of these substances, there is a need for rapid and sensitive techniques for chemical detection. Surface-enhanced Raman spectroscopy (SERS) has the potential for trace detection of harmful chemical substances. However, vibrational spectra obtained by SERS often differ between SERS substrates, as well as compared with spectra from normal Raman (NR) spectroscopy. Herein, SERS and NR responses from two fentanyl analogues, carfentanil (CF) and thiofentanil (TF), were measured and analysed with support from density functional theory (DFT) modelling. Using commercially available silver nanopillar SERS substrates, the SERS signatures of samples diluted in acetonitrile between 0.01 and 1000 µg/mL were studied. Relative SERS peak intensities measured in the range of 220–1800 cm−1 vary with concentration, while SERS and NR spectra largely agree for CF at higher concentrations (\u0000<math>\u0000 <mo>≥</mo></math>100 µg/mL). For TF, three distinct NR peaks at 262, 366 and 667 cm−1 are absent or strongly suppressed in the SERS spectrum, attributed to the lone-pair electrons of the thiophene's sulphur atom binding to the Ag surface. The concentration dependence of the Raman peak at \u0000<math>\u0000 <mo>∼</mo></math>1000 cm−1, assigned to trigonal bending of the phenyl ring, approximately follows a Langmuir adsorption isotherm. This work elucidates similarities and differences between SERS and NR in fentanyl detection and discusses the chemical rationale behind these differences.","PeriodicalId":16926,"journal":{"name":"Journal of Raman Spectroscopy","volume":"55 4","pages":"481-492"},"PeriodicalIF":2.5,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jrs.6643","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139423757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-pressure behavior and stability of synthetic buetschliite K2Ca(CO3)2 up to 19 GPa and 300°C 合成布氏体 K2Ca(CO3)2 在 19 GPa 和 300°C 下的高压行为和稳定性

IF 2.5 3区化学

Journal of Raman Spectroscopy Pub Date : 2024-01-10 DOI: 10.1002/jrs.6654

Anna Yu Likhacheva, Sofija Miloš, Alexandr V. Romanenko, Sergey V. Goryainov, Anna I. Semerikova, Sergey V. Rashchenko, Ronald Miletich, Anton Shatsky

{"title":"High-pressure behavior and stability of synthetic buetschliite K2Ca(CO3)2 up to 19 GPa and 300°C","authors":"Anna Yu Likhacheva, Sofija Miloš, Alexandr V. Romanenko, Sergey V. Goryainov, Anna I. Semerikova, Sergey V. Rashchenko, Ronald Miletich, Anton Shatsky","doi":"10.1002/jrs.6654","DOIUrl":"10.1002/jrs.6654","url":null,"abstract":"The occurrence of buetschliite, K2Ca(CO3)2, as inclusions in mantle minerals, is considered as one of the keys to understanding phase relationships of dense carbonates and outlines the potential role of potassium carbonates in the Earth's deep carbon cycle. Within this scope, the high-pressure behavior of synthetic buetschliite is characterized by in situ Raman spectroscopy up to 19 GPa and 300°C. Up to 6 GPa, the compression is regular, then the splitting of some of the lattice and internal modes defines the transition to a low-symmetry phase, in analogy to that observed previously in K2Mg(CO3)2. The temperature rise to 300°C shifts the transition pressure from ~6 to ~8 GPa, but on the whole, it does not change the high-pressure behavior of K2Ca(CO3)2. The observed pressure-induced spectral changes are fully reversible at room and elevated temperature. The findings show the expansion of buetschliite baric stability with temperature, which confirms its importance as a constituent of carbonate inclusions in deep minerals.","PeriodicalId":16926,"journal":{"name":"Journal of Raman Spectroscopy","volume":"55 4","pages":"517-524"},"PeriodicalIF":2.5,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139464839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A combined approach to the vibrational characterization of medieval paints on parchment: Handheld Raman spectroscopy and micro-SORS 对羊皮纸上的中世纪颜料进行振动表征的综合方法：手持式拉曼光谱和微型 SORS

IF 2.5 3区化学

Journal of Raman Spectroscopy Pub Date : 2024-01-09 DOI: 10.1002/jrs.6632

Márcia Vieira, Maria J. Melo, Claudia Conti, Federica Pozzi

{"title":"A combined approach to the vibrational characterization of medieval paints on parchment: Handheld Raman spectroscopy and micro-SORS","authors":"Márcia Vieira, Maria J. Melo, Claudia Conti, Federica Pozzi","doi":"10.1002/jrs.6632","DOIUrl":"10.1002/jrs.6632","url":null,"abstract":"A careful analytical study of the paint materials used in medieval illuminated manuscripts and their respective formulations is critical to their preservation. Due to their high value and fragile nature, access to these objects is often limited. Therefore, the development of ad-hoc methodologies allowing researchers to collect data in the least invasive way possible is an essential task in conservation science. This article shows the potential of a combined approach that complements handheld Raman spectroscopy with micro-SORS for the characterization of medieval paints. This methodology was tested on a reference collection of mock-up samples prepared as pure, mixture, and layered paints on parchment, based on historical information regarding paint formulations from Iberian scriptoria. Conventional Raman analysis, carried out by means of two handheld spectrometers, was found particularly effective for materials identification in pure paints and multi-component formulations of increasing complexity. Complementing this data, micro-SORS proved decisive in differentiating between mixture and layered paints in most mock-ups examined, yielding detailed information about the stratigraphy of reference samples produced through the overlaying of different paints. This combined methodology may be helpful to researchers who would like to approach the vibrational characterization of paints applied onto fragile artifacts and supports in a totally non-invasive manner.","PeriodicalId":16926,"journal":{"name":"Journal of Raman Spectroscopy","volume":"55 2","pages":"263-275"},"PeriodicalIF":2.5,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139420770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mass-correlated rotational Raman spectra and the structure of furan 质量相关旋转拉曼光谱和呋喃的结构

IF 2.5 3区化学

Journal of Raman Spectroscopy Pub Date : 2024-01-09 DOI: 10.1002/jrs.6629

Begüm Rukiye Özer, Jong Chan Lee, In Heo, Thomas Schultz

引用次数: 0

Peak-aware adaptive denoising for Raman spectroscopy based on machine learning approach 基于机器学习方法的拉曼光谱峰值感知自适应去噪技术

IF 2.5 3区化学

Journal of Raman Spectroscopy Pub Date : 2024-01-08 DOI: 10.1002/jrs.6648

Juhyung Lee, Woonghee Lee

引用次数: 0

Advancing clinical cancer care: Unveiling the power of surface-enhanced Raman spectroscopy 推进临床癌症治疗：揭开表面增强拉曼光谱的神秘面纱

IF 2.5 3区化学

Journal of Raman Spectroscopy Pub Date : 2024-01-04 DOI: 10.1002/jrs.6637

Xue Li, Guang Yang, Shuwen Zhang, He Ren

{"title":"Advancing clinical cancer care: Unveiling the power of surface-enhanced Raman spectroscopy","authors":"Xue Li, Guang Yang, Shuwen Zhang, He Ren","doi":"10.1002/jrs.6637","DOIUrl":"10.1002/jrs.6637","url":null,"abstract":"Tumors are characterized by abnormal cell growth, which leads to uncontrolled cell proliferation, invasion of surrounding tissues, and potential metastasis to other parts of the body. Current treatments for tumors include surgery, chemotherapy, radiotherapy, targeted therapy, and biological therapy. However, many tumors are detected at advanced stages, limiting the effectiveness of these treatments. Therefore, there is a need to develop more sensitive and efficient techniques to improve patient survival rates. Surface-enhanced Raman scattering (SERS) technology is a highly sensitive molecular detection and characterization technique. It can be combined with histomorphology, immunolabeling assays, and molecular hybridization to achieve quantitative diagnosis at morphological, molecular, and genetic levels. SERS offers advantages such as objectivity, reproducibility, and comparability. However, there are challenges to address, including complex substrate or probe preparation, cumbersome data analysis, and signal reproducibility and reliability. To fully utilize the potential of SERS in the biomedical field and for clinical translation, it is important to optimize the technology and develop more sensitive, accurate, and reliable substrate or probe preparation and analysis methods. This review aims to comprehensively explain the mechanism and unique advantages of SERS technology and discuss its current status, challenges, and prospects in tumor-related research.","PeriodicalId":16926,"journal":{"name":"Journal of Raman Spectroscopy","volume":"55 4","pages":"429-444"},"PeriodicalIF":2.5,"publicationDate":"2024-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139376005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0