Journal of Experimental and Clinical Pharmacy (JECP)最新文献

{"title":"Physical Characteristics of Processed Food Meatballs Based on Mussels (Anadara granosa)","authors":"Youstiana Dwi Rusita, Wynne Afra Shafira, M. Nasikin","doi":"10.52365/jecp.v3i1.621","DOIUrl":"https://doi.org/10.52365/jecp.v3i1.621","url":null,"abstract":"Seafood is great for consumption by various age groups because it is rich in protein, fat, vitamins and minerals. One of the seafood that is often used by the community is shellfish. Mussels (Anadara granosa) is a type of shellfish commonly eaten by Asian people because it has economic value and excellent nutritional content for consumption, especially a source of protein and minerals. One of the processed forms of mussels is meatball. The purpose of the study was to determine the results of physical quality (organoleptic and pH), chemical quality (fat and protein) in mussels meatballs (Anadara granosa). This types of research carried out quantitative research using descriptive. The test parameters are organoleptic test, pH test, protein content test using the biuret method and fat content test using the soxhletation method. The results of the organoleptic test showed a green-black color, a normal smell typical of meat, a normal taste for meatballs, a chewy texture and a pH of 6.807. The results of the protein content test in mussel meatballs were 2.972% and the fat content test had a level of 9.1186%. Based on the physical tests, mussels meatballs meet the criteria.","PeriodicalId":168977,"journal":{"name":"Journal of Experimental and Clinical Pharmacy (JECP)","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125928538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential Antibacterials of Merang Padi Extract (Oryza sativa) on the Growth of Gram Positive and Gram Negative Bacteria","authors":"Masniah Masniah, Rini Andarwati, A. Faisal, Mimin Wulandari","doi":"10.52365/jecp.v3i1.620","DOIUrl":"https://doi.org/10.52365/jecp.v3i1.620","url":null,"abstract":"Padi straw (Oryza sativa) is one type of stem produced from padi tree trunks. Antibacterial activity testing was carried out by agar diffusion method using paper disc as a backup by observing the formation of a clear zone indicating the presence of antibacterial activity. Variations in the concentration of the padi straw extract test solution were made with a %w/v ratio between 100% pure padi straw extract and DMSO adjusted at various test concentrations, namely, 5%, 10%, 15%, 20%, and 25%. The results showed that the n-hexane fraction of padi straw did not have antibacterial activity because there was no inhibition zone or kill zone indicated by the fraction at both low and high concentrations. While the ethanol extract showed antibacterial activity at a concentration of 15%, the ethyl acetate fraction and the n-butanol fraction had antibacterial activity indicated by the presence of an inhibitory zone at a concentration of 7%. The results of the t-test on the ethanol extract of the test bacteria obtained a Tcount value of 14,687 which means it is very significant or very significantly different because Tcount is greater than Ttable (5% and 1%). This shows that the oxytetracycline antibiotic is better than the potency of the ethanol extract of straw on the test bacteria.","PeriodicalId":168977,"journal":{"name":"Journal of Experimental and Clinical Pharmacy (JECP)","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125381449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aktivitas Antibakteri Ekstrak Buah Pare (Momordica charantia L.) Terhadap Bakteri Vibrio cholerae dan Bacillus cereus","authors":"N. Ismawati, Alfrida Monica Salasa, Dwi Rachmawaty Daswi","doi":"10.52365/jecp.v3i1.611","DOIUrl":"https://doi.org/10.52365/jecp.v3i1.611","url":null,"abstract":"Buah pare (Momordica charantia L.)  merupakan tanaman yang memiliki bau khas yang dimanfaatkan oleh masyarakat sebagai sayuran dan untuk pengobatan tradisional seperti batuk berdahak dan disentri. Buah pare memiliki kandungan senyawa alkaloid, flavonoid, saponin, tannin, dan triterpenoid yang dapat memiliki aktivitas sebagai antibakteri. Penelitian ini bertujuan untuk menentukan aktivitas antibakteri berdasarkan diameter zona hambat ekstrak buah pare terhadap Vibrio cholerae dan Bacillus cereus. Buah pare dikeringkan lalu di maserasi dengan pelarut etanol 96% dilanjutkan uji skrinning fitokimia dan uji aktivitas antibakteri dengan metode disc diffusion konsentrasi ekstrak 2%, 4%, 8% kontrol positif menggunakan antibiotik kloramfenikol dan kontrol negatif DMSO. Hasil penelitian menunjukkan ekstrak buah pare memiliki aktivitas terhadap bakteri Vibrio cholerae dan Bacillus cereus dan konsentrasi yang memiliki zona hambat terbesar adalah konsentrasi 8%.Bitter gourd fruit (Momordica charantia L.) is a plant that has a distinctive smell and is used by the community as a vegetable and for traditional medicine such as cough with phlegm and dysentery. Bitter gourd contains alkaloids, flavonoids, saponins, tannins, and triterpenoids that have antibacterial activity. This study aims to determine the antibacterial activity based on the diameter of the inhibition zone of bitter melon extract against Vibrio cholerae and Bacillus cereus. Bitter gourd fruit was dried and then macerated with 96% ethanol as a solvent, followed by phytochemical screening tests and antibacterial activity tests using the disc diffusion method with extract concentrations of 2%, 4%, and 8% as positive controls using chloramphenicol antibiotics and DMSO as a negative control. The results showed that bitter melon extract had activity against the bacteria Vibrio cholerae and Bacillus cereus, and the concentration that had the largest inhibition zone was 8%.","PeriodicalId":168977,"journal":{"name":"Journal of Experimental and Clinical Pharmacy (JECP)","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131324996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efektifitas Pemberian Minyak Kuda (Equus caballus) untuk Mempercepat Penyembuhan Luka Pada Kelinci Jantan (Oryctolagus cuniculus)","authors":"Nur Intang, Hendra Stevani, R. Dewi","doi":"10.52365/jecp.v3i1.432","DOIUrl":"https://doi.org/10.52365/jecp.v3i1.432","url":null,"abstract":"Minyak kuda (Equus caballus) sering digunakan oleh masyarakat Jeneponto untuk menyembuhkan luka, namun bukti ilmiah tentang hal tersebut masih kurang. Tujuan penelitian ini adalah untuk mengetahui efektifitas dari minyak kuda pada penyembuhan luka kelinci jantan (Oryctolagus cuniculus). Minyak kuda diperoleh dari lemak kuda pada bagian perutnya yang dibuat dengan metode destilasi uap air. Minyak yang diperoleh kemudian diberikan pada kelinci jantan yang terlebih dahulu diberikan luka sepanjang 3 cm dengan kedalaman 0,2 cm, kemudian untuk pembanding digunakan betadin dan sebagai kontrol negatif tidak diberi perlakuan. Pengamatan dilakukan selama 7 hari dengan melihat perubahan panjang luka pada kelinci jantan. Hasil penelitian menunjukkan bahwa terjadi penurunan luka yang signifikan antara tiap perlakuan dimana pada hari ke-7 rata-rata menunjukkan adanya perubahan namun kelompok perlakuan dengan pemberian minyak kuda lukanya sudah sembuh dibandingkan dengan betadin dan kontrol negatif. Berdasarkan hasil penelitian dan analisis data maka dapat disimpulkan bahwa  pemberian minyak kuda murni efektif dalam menyembuhkan luka kelinci jantan namun efeknya tidak jauh berbeda dengan betadin.Horse oil (Equus caballus) is often used by the people of Jeneponto to heal wounds, but scientific evidence regarding this is still lacking. The purpose of this study was to determine the effectiveness of horse oil on wound healing in male rabbits (Oryctolagus cuniculus). Horse oil is obtained from horse fat in the stomach, which is made by the steam distillation method. The oil obtained was then given to male rabbits, which were first given a 3 cm wound with a depth of 0.2 cm, then betadine was used as a comparison, and as a negative control, they were not given any treatment. Observations were made for 7 days by observing changes in the length of the wound in male rabbits. The results showed that there was a significant reduction in wound size between each treatment; on the 7th day, the average showed a change, but the treatment group with horse oil had healed compared to betadine and negative controls. Based on the results of the research and data analysis, it can be concluded that giving pure horse oil is effective in healing the wounds of male rabbits, but the effect is not much different from betadine.","PeriodicalId":168977,"journal":{"name":"Journal of Experimental and Clinical Pharmacy (JECP)","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133187745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tinjauan Literatur: Potensi Tanaman Famili Apiaceae sebagai Imunomodulator dan Antioksidan","authors":"Deani Sucia Fakhira, Nova Patricia, Novia Saumi","doi":"10.52365/jecp.v3i1.422","DOIUrl":"https://doi.org/10.52365/jecp.v3i1.422","url":null,"abstract":"Sistem imun merupakan sebuah sistem untuk mempertahankan dan melindungi keutuhan tubuh yang dapat menyerang sel-sel dalam tubuh. Penyakit yang berhubungan dengan sistem imun diantaranya HIV, Covid-19, dan influenza. Suatu zat yang dapat merangsang, menekan atau memodulasi salah satu dari komponen sistem kekebalan tubuh disebut imunomodulator. Penelitian ini dilakukan untuk mengetahui kandungan kimia dan mekanisme zat aktif dari kelompok tanaman famili Apiaceae sebagai imunomodulator dan antioksidan. Metode yang digunakan dalam penelitian ini yaitu penelitian kualitatif dengan metode tinjauan literatur sistematis. Hasil penelaahan pustaka famili Apiaceae memiliki aktivitas kandungan sebagai imunomodulator dan antioksidan diantaranya Daucus carota (wortel), Apii graveolus (seledri), Centella asiatica (pegagan), Nigella sativa (jinten), Foenicullum vulgare (adas hitam), dan Pimpinella anisum (adas manis) dengan kandungan flavonoid, saponin, tanin, dan vitamin C yang bekerja meningkatkan respon imun terhadap sistem kekebalan tubuh. The immune system is a system to maintain and protect the integrity of the body that can attack cells in the body. Diseases related to the immune system include HIV, Covid-19, and influenza. A substance that can stimulate, suppress or modulate one of the components of the immune system is called an imunomodulator. This research was conducted to determine the chemical content and mechanism of active substances from the Apiaceae family of plants as imunomodulators and antioxidants. The method used in this study is qualitative research using a systematic literature review method. The results of a literature review of the Apiaceae family have content activity as immunostimulants and antioxidants including Daucus carota (carrot), Apii graveolus (celery), Centella asiatica (gotu kola), Nigella sativa (cumin), Foenicullum vulgare (black fennel), and Pimpinella anisum (aniseed) contains flavonoids, saponins, tannins, and vitamin C which work to enhance the immune response against the immune system.","PeriodicalId":168977,"journal":{"name":"Journal of Experimental and Clinical Pharmacy (JECP)","volume":"150 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132030143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulasi dan Uji Mutu Sediaan Sabun Padat Ekstrak Daun Pisang Kepok (Musa paradisiaca Linn.) dengan Variasi Konsentrasi Minyak Kelapa","authors":"Mayang Aditya Ayuning Siwi, Fendy Yoga Wardhana, Dwi Septiawati","doi":"10.52365/jecp.v3i1.466","DOIUrl":"https://doi.org/10.52365/jecp.v3i1.466","url":null,"abstract":"Sabun padat akan bernilai jual lebih jika diperkaya pula dengan bahan natural. Ekstrak daun pisang kepok (Musa paradisiaca Linn.) mengandung senyawa flavonoid, saponin dan tannin yang dapat digunakan sebagai senyawa antioksidan sehingga memungkinkan untuk digunakan sebagai bahan aktif pada pembuatan sabun padat. Tujuan penelitian ini adalah untuk memformulasikan ekstrak daun pisang kepok menjadi sabun padat dan melakukan uji mutu sediaan. Metode yang digunakan adalah metode eksperimental. Formulasi sabun padat menggunakan variasi konsentrasi minyak kelapa 25% dan 30%. Uji mutu yang dilakukan yaitu uji organoleptis, uji pH, uji kadar air, uji tinggi dan stabilitas busa. Pengujian dilakukan pada hari ke-1 dan hari ke-7. Hasil uji organoleptis menunjukkan bahwa bau sediaan stabil. Hasil uji pH, uji kadar air, uji tinggi dan stabilitas busa semakin baik pada hari ke-7 penyimpanan. Kesimpulan dari penelitian ini adalah ekstrak daun pisang kepok dapat diformulasikan menjadi sediaan sabun padat dan sabun padat yang dibuat telah memenuhi syarat uji mutu.Solid soap will be more valuable if it is also enriched with natural ingredients. Kepok banana leaf extract (Musa paradisiaca Linn.) contains flavonoids, saponins, and tannins that can be used as antioxidant compounds, making it possible to use them as active ingredients in the manufacture of solid soap. The purpose of this study was to formulate Kepok banana leaf extract into solid soap and test the quality of the preparation. The method used is the experimental method. Solid soap formulation using various concentrations of coconut oil 25% and 30%. The quality tests carried out were organoleptic tests, pH tests, moisture content tests, height and stability of foam tests. Tests were carried out on day 1 and day 7. The organoleptic test results showed that the odor of the preparation was stable. The results of the pH test, water content test, height and stability of foam test were getting better on the 7th day of storage. The conclusion of this study is that Kepok banana leaf extract can be formulated into solid soap preparations, and the solid soap made has met the quality test requirements.","PeriodicalId":168977,"journal":{"name":"Journal of Experimental and Clinical Pharmacy (JECP)","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127793411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pengaruh Dekokta Akar Putri Malu (Mimosa Pudica Linn.) Terhadap Fertilitas pada Tikus Putih Betina Dibandingkan dengan Pil KB","authors":"Ismedsyah Ismedsyah, Lavinur Lavinur, M. Rifqi","doi":"10.52365/jecp.v2i2.434","DOIUrl":"https://doi.org/10.52365/jecp.v2i2.434","url":null,"abstract":"Kepadatan penduduk berdampak pada kualitas penduduk yang menurun dibidang pendidikan, kesehatan, pendapatan maupun pekerjaan. Kepadatan penduduk dikarenakan kelahiran tidak terencana, semakin tinggi angka kelahiran maka semakin padat penduduk. Untuk membatasi angka kelahiran yang semakin tinggi, diperlukan program keluarga berencana. Tujuan penelitian ini untuk mengetahui pengaruh dekokta akar putri malu terhadap panjang siklus estrus tikus betina. Jenis penelitian yaitu eksperimental pengaruh dekokta akar putri malu terhadap panjang siklus estrus sebagai antifertilitas. Rancangan penelitian acak lengkap dengan menggunakan tikus putih betina galur wistar 5 kelompok dengan 5 ulangan. Kelompok kontrol negatif diberikan aquadest, kontrol positif diberikan pil KB, P1 diberikan dekokta akar putri malu dengan dosis 100 mg/kg BB, P2 diberikan dosis 200 mg/kg BB, dan P3 diberikan dosis 300 mg/kg BB. Pemberian kontrol negatif, positif dan perlakuan secara oral sekali sehari selama 15 hari. Pengamatan dilakukan setiap hari jam 08.00 WIB dengan apus vagina. Data diuji Analisis Varians (ANOVA), dilanjutkan dengan uji Duncan Multiple Range Test (DMRT). Hasil penelitian dekokta akar putri malu mempengaruhi panjang siklus estrus tikus sehingga dapat diangggap efektif sebagai antifertilitas. Dosis yang dianggap efektif sebagai antifertilitas sesuai kontrol positif yaitu didapat pada dosis 300 mg/kg BB.Population density has an impact on the declining quality of the population in the fields of education, health, income and employment. Population density due to unplanned births, the higher the birth rate, the denser the population. To limit the higher birth rate, a family planning program is needed. The purpose of this study was to determine the effect of the root of Putri Malu on the estrus cycle length of female rats. The type of research is experimental, the effect of the root of Putri Malu on the length of the estrus cycle as an antifertility agent. Completely randomized study design using female white rats wistar strain 5 groups with 5 replications. The negative control group was given aquadest, the positive control was given birth control pills, P1 was given a decoction of the roots of putri malu at a dose of 100 mg/kg BW, P2 was given a dose of 200 mg/kg WW, and P3 was given a dose of 300 mg/kg WW. Giving negative control, positive and oral treatment once a day for 15 days. Observations were made every day at 08.00 WIB with vaginal smears . The data were tested by Analysis of Variance (ANOVA), followed by the Duncan Multiple Range Test (DMRT). The results of this study were that the root of Putri malu's roots affected the length of the rat's estrous cycle so that it could be considered effective as an antifertility agent. The dose that was considered effective as antifertility according to the positive control was obtained at a dose of 300 mg/kg BW.","PeriodicalId":168977,"journal":{"name":"Journal of Experimental and Clinical Pharmacy (JECP)","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128503515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efektifitas Antihiperglikemik Ekstrak Daun Salvia (Salvia occidentalis Sw.) Terhadap Mencit (Mus musculus)","authors":"Lisda Lisda, Sisilia Tresia Rosmala Dewi, Sesilia Rante Pakadang","doi":"10.52365/jecp.v2i2.429","DOIUrl":"https://doi.org/10.52365/jecp.v2i2.429","url":null,"abstract":"Daun salvia (Salvia occidentalis Sw.) merupakan salah satu obat tradisional untuk menyembuhkan penyakit secara turun-temurun, yang diyakini memiliki khasiat untuk menyembuhkan penyakit salah satu DM (diabetes melitus). Diketahui mengandung senyawa kimia yang berupa alkaloid, flavonoid, tannin, polifenol dan saponin. Penelitian ini bertujuan untuk mengetahui efektifitas ekstrak daun salvia (Salvia occidentalis Sw.) sebagai Antihiperglikemik terhadap Mencit (Mus muculus). Daun salvia (Salvia occidentalis Sw.) diekstraksi dengan mengunakan metode maserasi, dan diuapkan menggunakan rotavapor. Analisis statistik dilakukan dengan menggunakan Statistica Produce and Service Solution (SPSS) dan ANOVA. Hasil analisis menunjukkan data berdistribusi normal dan homogen dengan nilai normalitas 0,843 (p 0,05) dan nilai homogenitas 0,526 (p≥0,05). Sehingga dilakukan dengan uji parametik ANOVA. Hasil analisis menunjukkan nilai signifikan 0,000(p≤0,05) sehingga daun salvia (Salvia occidentalis Sw.) terbukti dapat menurunkan kadar gulukosa darah pada mencit yang diinduksi aloksan pada konsentrasi 5%,10% dan 15% dan didapatkan nilai yang paling optimal dalam penurunan glukosa dara pada mencit (Mus muculus) adalah konsentrasi 5%.Salvia leaf ( Salvia occidentalis Sw.) is one of the traditional medicines for curing diseases from generation to generation, which is believed to have efficacy to cure diabetes mellitus (DM). It is known to contain chemical compounds in the form of alkaloids, flavonoids, tannins, polyphenols and saponins. This study aims to determine the effectiveness of salvia leaf extract ( Salvia occidentalis Sw.) as an antihyperglycemic against mice ( Mus muculus ). The leaves of salvia ( Salvia occidentalis Sw.) were extracted using the maceration method, and evaporated using a rotary evaporator. Statistical analysis was performed using Statistica Produce and Service Solution (SPSS) and ANOVA . The results of the analysis showed that the data were normally distributed and homogeneous with a normality value of 0.843 (p 0.05 ) and a homogeneity value of 0.526 (p≥0.05). So it was carried out with the parametric ANOVA test . The results of the analysis showed a significant value of 0.000 (p≤0.05) so that salvia leaf ( Salvia occidentalis Sw.) was proven to reduce blood glucose levels in alloxan-induced mice at concentrations of 5%, 10% and 15% and the most optimal value was obtained in decrease in blood glucose in mice ( Mus muculus ) is a concentration of 5%.","PeriodicalId":168977,"journal":{"name":"Journal of Experimental and Clinical Pharmacy (JECP)","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128500311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skrining Fitokimia dan Potensi Antimikroba Herba Kemangi (Ocimum americanum L.) Terhadap Staphylococcus aureus, Salmonella typhi dan Candida albicans","authors":"P. Anggraini, St. Ratnah, Sesilia TR Dewi","doi":"10.52365/jecp.v2i2.423","DOIUrl":"https://doi.org/10.52365/jecp.v2i2.423","url":null,"abstract":"Ocimum spp. merupakan salah satu tanaman yang digunakan sebagai bahan obat tradisional, karena memiliki senyawa antioksidan, antikanker dan antimikroba. Tujuan penelitian ini adalah untuk mengetahui senyawa fitokimia dan potensi antimikroba ekstrak Herba Kemangi (Ocimum americanum L.) terhadap Staphylococcus aureus, Salmonella typhi dan Candida albicans berdasarkan zona hambat. Ekstraksi dilakukan dengan maserasi menggunakan etanol 96%. Metode kerja yang digunakan yaitu pembuatan simplisia, ekstraksi, skrining fitokimia, pengujian aktivitas antimikroba dengan metode disc diffusion. maka diperoleh rata-rata zona hambat pada bakteri Staphylococcus aureus konsentrasi 2% b/v sebesar 11,3 mm, 4% b/v sebesar 14,3mm, 8% b/v sebesar 17,6 mm, kontrol positif (clindamycin) sebesar 26 mm dan kontrol negatif 0 mm. Pada bakteri Salmonella typhi konsentrasi 2% b/v sebesar 12,6 mm, 4% b/v sebesar 15,6 mm, 8% b/v sebesar 19,3 mm, kontrol positif (ciprofloxacin) sebesar 29,3 mm dan kontrol negatif 0 mm. Pada Candida albicans konsentrasi 2% b/v sebesar 0 mm, 4% b/v sebesar 6,6 mm, 8% b/v sebesar 8,3 mm, kontrol positif (nystatin) sebesar 12,3 mm dan kontrol negatif 0 mm.Ocimum spp. is one of the plants used as ingredients in traditional medicine, because it has antioxidant, anticancer and antimicrobial compounds. The purpose of this study was to determine the phytochemical compounds and antimicrobial potential of the Herba Basil extract ( Ocimum americanum L.) against Staphylococcus aureus , Salmonella typhi and Candida albicans based on the zone of inhibition. Extraction was carried out by maceration using 96% ethanol. The working method used is the manufacture of simplicia, extraction, phytochemical screening, antimicrobial activity testing with the disc diffusion method . the average inhibition zone of Staphylococcus aureus was 11.3 mm, 4% w/v was 14.3 mm, 8% w/v was 17.6 mm, positive control (clindamycin) was 26 mm and 0 mm negative control. In Salmonella typhi the concentration of 2% w/v was 12.6 mm, 4% w/v was 15.6 mm, 8% w/v was 19.3 mm, positive control (ciprofloxacin) was 29.3 mm and control negative 0 mm. In Candida albicans the concentration of 2% w/v was 0 mm, 4% w/v was 6.6 mm , 8% w/v was 8.3 mm, positive control (nystatin) was 12.3 mm and negative control was 0 mm.","PeriodicalId":168977,"journal":{"name":"Journal of Experimental and Clinical Pharmacy (JECP)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129182833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimasi dan Validasi Metode KCKT untuk Identifikasi dan Penetapan Kadar Vitamin B1, B3, B6, dan Kofein dalam Suplemen Kesehatan","authors":"Sabaruddin Sabaruddin, Umy Kurniati Rasyid, Whyllies Agung Buana, Mieke Pakaya, Fitriana Nur Husain","doi":"10.52365/jecp.v2i2.425","DOIUrl":"https://doi.org/10.52365/jecp.v2i2.425","url":null,"abstract":"Uji identifikasi dan penetapan kadar merupakan salah satu persyaratan mutu suplemen kesehatan yang mengandung vitamin B1, B3, B6, dan kofein sehingga diperlukan metode analisis yang optimal untuk mendeteksi secara simultan analit-analit tersebut dalam suatu campuran matriks. Saat ini telah tersedia metode analisa KCKT sistem gradien untuk pengujian tersebut. Namun, dari metode analisa tersebut diperoleh profil kromatogram yang belum optimal dengan nilai resolusi 0,844 pada puncak analit vitamin B3 dan B1. Hal ini disebabkan masih berhimpitnya puncak kedua analit dan tidak terpisah dengan baik pada baseline kromatogram. Oleh karena itu dianggap perlu untuk melakukan optimasi pada metode analisa ini dengan melakukan modifikasi perbandingan komposisi fase gerak sistem gradien pada menit terelusinya vitamin B3 dan B1. Metodologi penelitian menggunakan penelitian eksperimental laboratorium dengan parameter yang diamati adalah profil kromatogram pemisahan puncak analit dan nilai resolusi antara vitamin B3 dan B1. Berdasarkan data hasil optimasi diperoleh komposisi fase gerak ammonium asetat : asetonitril (25:75) yang memberikan profil kromatogram optimal terhadap pemisahan vitamin B3 dan B1 dengan nilai resolusi 7,230 untuk vitamin B3 dan 3,427 untuk vitamin B1 (syarat resolusi > 2). Metode optimasi kemudian dilanjutkan dengan validasi metode analisa dengan hasil uji parameter selektifitas, akurasi, presisi, linearitas, nilai LOD, dan nilai LOQ memenuhi syarat keberterimaan sehingga metode ini dapat digunakan untuk melakukan pengujian identifikasi dan penetapan kadar vitamin B1, B3, B6, dan kofein dalam sediaan suplemen kesehatan.Identification and assay tests are one of the quality requirements of health supplements containing vitamins B1, B3, B6, and caffeine, so an optimal analytical method is needed to detect these analytes simultaneously in a matrix mixture. Currently, a gradient system HPLC analysis method is available for this test. However, from this analysis method, the chromatogram profile was not optimal with a resolution value of 0.844 at the peak of the vitamin B3 and B1 analytes. This is because the peaks of the two analytes are still close together and are not well separated on the baseline chromatogram. Therefore, it is considered necessary to optimize this analytical method by modifying the ratio of the mobile phase composition of the gradient system at the minute of elution of vitamins B3 and B1. The research methodology used experimental laboratory research with the parameters observed were the chromatogram profile of analyte peak separation and the resolution value between vitamins B3 and B1. Based on the optimization data, the mobile phase composition of ammonium acetate: acetonitrile (25:75) provides an optimal chromatogram profile for the separation of vitamins B3 and B1 with a resolution value of 7,230 for vitamin B3 and 3,427 for vitamin B1 (resolution > 2). The optimization method is then followed by validation of the analytic","PeriodicalId":168977,"journal":{"name":"Journal of Experimental and Clinical Pharmacy (JECP)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131251415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}