Journal of Plant Diseases and Protection最新文献_第3页

Effect of plantain barrier plants on potyvirus-associated diseases in yam cultivation 车前草屏障植物对山药种植中潜在病毒相关疾病的影响

IF 2 4区农林科学

Journal of Plant Diseases and Protection Pub Date : 2024-08-01 DOI: 10.1007/s41348-024-00948-4

José Efraín González Ramírez, Vaniert Ventura Chávez, Alberto Fereres, Orelvis Portal

{"title":"Effect of plantain barrier plants on potyvirus-associated diseases in yam cultivation","authors":"José Efraín González Ramírez, Vaniert Ventura Chávez, Alberto Fereres, Orelvis Portal","doi":"10.1007/s41348-024-00948-4","DOIUrl":"https://doi.org/10.1007/s41348-024-00948-4","url":null,"abstract":"Yam belongs to a very diverse plant genus (Dioscorea L.) that includes more than 600 species, both wild and cultivated. In Cuba, different species are distributed in the central and eastern regions, where the most widespread cultivars belong to the species water yam (Dioscorea alata L.) and white yam (Dioscorea cayenensis subsp. rotundata (Poir.) J. Miège). The major constraints faced by yam production areas are those caused by viral diseases. In this sense, potyviruses have the greatest economic impact, since they can cause losses of more than 50% in agricultural yields. The movement of propagules between plantations without phytosanitary certification has facilitated the spread of viruses throughout the production areas. Understanding the relationships between the host, virus, vector, and ecosystem is fundamental for successful disease management and for increasing agricultural yields. The objective of the present work was to determine the effect of using virus-free planting material and plantain barriers on the production of two yam cultivars. For this purpose, potyvirus-free certified and non-certified planting material were evaluated inside and outside a live barrier based on plantain ‘Burro CEMSA’. The virus incidence and production of these plants were determined in each of the experiments conducted. It was possible to reduce the field incidence of viral diseases (average infection decreased from 60 to 15%). The barrier also prevented yield losses of 50–57% for both cultivars, compared to the farmer’s usual practices.","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"32 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141881994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Load of the ash dieback pathogen hymenoscyphus fraxineus differs in soil 土壤中白蜡树枯死病原体hymenoscyphus fraxineus的负荷量不同

IF 2 4区农林科学

Journal of Plant Diseases and Protection Pub Date : 2024-07-31 DOI: 10.1007/s41348-024-00970-6

Jan Werner Böhm, Christina Zübert, Georgia Kahlenberg, Susanne Jochner-Oette, Michael Kube

{"title":"Load of the ash dieback pathogen hymenoscyphus fraxineus differs in soil","authors":"Jan Werner Böhm, Christina Zübert, Georgia Kahlenberg, Susanne Jochner-Oette, Michael Kube","doi":"10.1007/s41348-024-00970-6","DOIUrl":"https://doi.org/10.1007/s41348-024-00970-6","url":null,"abstract":"The ascomycete Hymenoscyphus fraxineus causes the devastating ash dieback disease of European ash (Fraxinus excelsior L.). Spore traps are often used to measure the amount of ascospores in the environment, but the pathogen-load of the soil in ash stands has not been recorded so far. This is of particular interest with regard to the occurrence of ash stem necrosis, a decisive factor for the severe course of the disease. In order to gain a more differentiated insight into the pathogen-load in ash stands, we analysed soil samples from four ash tree sites in southern Germany, covering a clone plantation, two seed orchards and a forest. The pathogen-load was determined using a quantitative TaqMan real-time PCR assay for ten to twenty plots per stand. Results obtained by the species-specific assay highlighted that the pathogen-load is heterogeneously distributed in the ash stands. H. fraxineus DNA targets were detected in 17% of the soil samples. The pathogen-load differed according to soil depth, with the highest pathogen abundance in the top 5 cm, followed by 5–10 cm and finally 10–15 cm. Pathogen-load and thereby infection pressure were found to be highly variable for the individual trees in one stand. Overall, the study discovered detectable levels of H. fraxineus in the soil of all four study sites, which supports the hypothesis that H. fraxineus can be found in the soil of ash stands. The qPCR approach was found to be an effective method for monitoring the load of H. fraxineus in soil and for demonstrating the successful application of the method on the sample type of custom-made spore traps. Results suggest the implication of site-specific pathogen-load determination in future H. fraxineus-monitoring and selection of less susceptible ash trees for breeding and seed production.","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"21 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141872098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unintended sprouts as additional resource for pathogen-free seed potato (Solanum tuberosum) propagation 意外萌芽作为无病原体种薯（Solanum tuberosum）繁殖的额外资源

IF 2 4区农林科学

Journal of Plant Diseases and Protection Pub Date : 2024-07-31 DOI: 10.1007/s41348-024-00975-1

José Alberto Caram de Souza-Dias, Falko Feldmann

引用次数: 0

Efficacy assessment in crop protection: a tutorial on the use of Abbott’s formula 作物保护的功效评估：雅培公式使用教程

IF 2 4区农林科学

Journal of Plant Diseases and Protection Pub Date : 2024-07-30 DOI: 10.1007/s41348-024-00968-0

Hans-Peter Piepho, Waqas Ahmed Malik, Robert Bischoff, Abbas El-Hasan, Christian Scheer, Jan Erik Sedlmeier, Roland Gerhards, Georg Petschenka, Ralf T. Voegele

引用次数: 0

Comparative biochemical and physiological responses to the virus-induced mosaic disease in apple (Malus domestica) 苹果（Malus domestica）对病毒诱发的花叶病的生化和生理反应比较

IF 2 4区农林科学

Journal of Plant Diseases and Protection Pub Date : 2024-07-29 DOI: 10.1007/s41348-024-00974-2

Subaya Manzoor, Sajad Un Nabi, Tariq Rasool Rather, M. K. Verma, Shouaa A. Alrobaish, Wasim H. Raja, Javid I. Mir, Tanveer Ahmad Wani, Riyazul Rouf Mir, Faheem Jeelani, Shugufta Parveen, Sheikh Mansoor

{"title":"Comparative biochemical and physiological responses to the virus-induced mosaic disease in apple (Malus domestica)","authors":"Subaya Manzoor, Sajad Un Nabi, Tariq Rasool Rather, M. K. Verma, Shouaa A. Alrobaish, Wasim H. Raja, Javid I. Mir, Tanveer Ahmad Wani, Riyazul Rouf Mir, Faheem Jeelani, Shugufta Parveen, Sheikh Mansoor","doi":"10.1007/s41348-024-00974-2","DOIUrl":"https://doi.org/10.1007/s41348-024-00974-2","url":null,"abstract":"The apple crop, integral to global agriculture, is facing a significant challenge in the form of mosaic disease, reducing productivity, particularly in temperate regions of the world. The intricate biochemical and physiological responses of apple genotypes to mosaic infection induced by apple mosaic virus (ApMV)/apple necrotic mosaic virus (ApNMV) were investigated in this study. Higher phenolic contents, DPPH, flavonoids and flavanols were recorded from mosaic-infected apple genotypes in comparison with their respective counterparts. The impact on carbohydrates/total starch and chlorophyll content revealed a substantial reduction in mosaic-infected as compared to healthy leaves, which emerges as a quantitative biomarker for measuring infection severity and offering insights into the compromised photosynthetic ability of infected leaves. In mosaic-infected genotypes, PAL activity, malondialdehyde and antioxidant enzymes were greater, strengthening the powerful antioxidative defense mechanism against mosaic infection. Principal component analysis provided a comprehensive overview, identifying key biomarkers and elucidating the underlying biochemical processes linked with apple mosaic disease. This research provides significant insights for future apple breeding programs by providing a more detailed knowledge of the biochemical intricacies underpinning plant defense systems against mosaic infection in apples.","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"26 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141872099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of biochar and wood vinegar from guava trees in controlling Fusarium verticillioides and enhancing growth and anatomical traits of maize (Zea mays L.) 番石榴树的生物碳和木醋在控制疣状镰刀菌和提高玉米（Zea mays L.）的生长和解剖性状方面的效果

IF 2 4区农林科学

Journal of Plant Diseases and Protection Pub Date : 2024-07-29 DOI: 10.1007/s41348-024-00971-5

Mansour M. El-Fawy, Ayman S. Saeed, Mohamed K. Abou-Shlell, Mahmoud A. Soliman, Esmat F. Ali, Ahmed A. Issa, Kamal A. M. Abo-Elyousr, Muhammad Imran, Asmaa S. El-Nagar

{"title":"Effectiveness of biochar and wood vinegar from guava trees in controlling Fusarium verticillioides and enhancing growth and anatomical traits of maize (Zea mays L.)","authors":"Mansour M. El-Fawy, Ayman S. Saeed, Mohamed K. Abou-Shlell, Mahmoud A. Soliman, Esmat F. Ali, Ahmed A. Issa, Kamal A. M. Abo-Elyousr, Muhammad Imran, Asmaa S. El-Nagar","doi":"10.1007/s41348-024-00971-5","DOIUrl":"https://doi.org/10.1007/s41348-024-00971-5","url":null,"abstract":"Maize (Zea mays L.) stalk and ear rots are the most serious diseases, affecting grain yield and quality in a number of regions of the world. The purpose of this study is to evaluate the efficacy of biochar and wood vinegar from guava trees (Psidium guajava L.) against Fusarium verticillioides, the pathogen that causes stalk and ear rot diseases, as well as the growth and anatomical traits of maize in laboratory and greenhouse experiments. In in vitro studies, biochar and wood vinegar at concentrations of 0.50, 1.00 and 2.00% (v/v) were tested on the mycelium growth of the pathogen. The results showed that wood vinegar had a high ability to inhibit the pathogen’s growth at a concentration of 2% (78.32%). Treating corn cobs with these treatments at concentrations of 2% led to a decrease in the severity of ear rot. Soil drenching with biochar and wood vinegar reduced the disease severity of stalk rot disease in maize in greenhouse experiments, with wood vinegar being the most effective in reducing the disease severity compared to the control. Biochar and wood vinegar both clearly affected plant growth, as measured by changes in plant height, fresh weight and dry weight of maize plants. Furthermore, these treatments enhanced the anatomical characteristics of maize roots and leaves compared to the untreated control plants. Data also revealed that total phenol contents and enzyme activity of peroxidase (POD) increased in treated plants with these treatments, but the highest increase was obtained when biochar was applied. These results highlight the efficacy of biochar and wood vinegar as effective, safe and eco-friendly methods to control of stalk and ear rot disease of maize and improve the anatomical characteristics and growth of plants.","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"24 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141871897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acaricidal efficacy of aqueous extracts from different plants on Tetranychus urticae Koch, 1836 (Acari: Tetranychidae) 不同植物的水提取物对 Tetranychus urticae Koch, 1836（蛔虫：Tetranychidae）的杀蛔功效

IF 2 4区农林科学

Journal of Plant Diseases and Protection Pub Date : 2024-07-29 DOI: 10.1007/s41348-024-00969-z

Yasin Nazım Alpkent, Selçuk Ulusoy, Sait Ertürk

{"title":"Acaricidal efficacy of aqueous extracts from different plants on Tetranychus urticae Koch, 1836 (Acari: Tetranychidae)","authors":"Yasin Nazım Alpkent, Selçuk Ulusoy, Sait Ertürk","doi":"10.1007/s41348-024-00969-z","DOIUrl":"https://doi.org/10.1007/s41348-024-00969-z","url":null,"abstract":"Two-spotted spider mite (Tetranychus urticae Koch, 1836, Acari: Tetranychidae) causes significant yield losses in cultivated plants, especially depending on the season. Chemical methods are the main methods of controlling this pest. However, besides the environmental, human, and animal health problems arising from chemical control, adverse effects such as resistance and residues limit the success and sustainability of chemical control. This experiment was conducted in the applied acarology laboratory of the Directorate of Plant Protection Central Research Institute in Ankara, Türkiye, in incubators maintained at 25 ± 1 °C temperature, 50–65% humidity, and 16 L: 8 D h photoperiod. In this study, the potential acaricidal effect of aqueous extracts of Morus rubra, Daphne odora, Ficus carica, Matricaria chamomilla, and Mentha pulegium collected from Ankara and Adana provinces on T. urticae was investigated. For this purpose, the leaves of the plants were dried and ground and 1%, 3%, 6%, and 12% concentrations (w/v) of the extracts obtained were tested in three different application periods (1–6 day). Dipping and spraying methods were used to determine mortality and toxicity. The highest effect was 94.4% at the end of the 6th day at a 12% concentration of M. pulegium in the dipping method. On the other hand, the lowest mortality effect was 18.2% at the same time and concentration of M. chamomilla. In lethal toxicity studies, the highest toxicity was obtained from F. carica with an LC50 value of 4756 mg L−1, and the lowest toxicity was obtained from D. odora with an LC50 value of 12,417 mg L−1. We believe that plant extracts provide a valid alternative in effectively controlling two-spotted spider mites, reducing the need for pesticide use and minimizing residues.","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"49 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141872097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anthracnose changes the diversity and composition of rhizosphere soil microbial community in common vetch 炭疽病改变了普通草本植物根瘤土壤微生物群落的多样性和组成

IF 2 4区农林科学

Journal of Plant Diseases and Protection Pub Date : 2024-07-17 DOI: 10.1007/s41348-024-00967-1

Qiong Wang, Rui Zhu, Faxi Li, Yingde Li, Meiting Bai, Tingyu Duan

{"title":"Anthracnose changes the diversity and composition of rhizosphere soil microbial community in common vetch","authors":"Qiong Wang, Rui Zhu, Faxi Li, Yingde Li, Meiting Bai, Tingyu Duan","doi":"10.1007/s41348-024-00967-1","DOIUrl":"https://doi.org/10.1007/s41348-024-00967-1","url":null,"abstract":"Anthracnose caused by Colletotrichum is an increasingly severe disease of common vetch that reduces plant yield and quality. In this study, the effects of anthracnose on the rhizosphere soil microbial community of common vetch were studied via high-throughput sequencing. The relationship among the rhizosphere soil microbes and soil properties were analyzed. Lower fungal diversity was observed in rhizosphere soils of diseased plants. Variations in relative abundance were observed in the fungal phyla Mortierellomycota and Glomeromycota and in the bacterial phyla Acidobacteria, Oxyphotobacteria, and Verrucomicrobia. Gammaproteobacteria and Deltaproteobacteria were significantly more abundant in the rhizosphere soil of healthy plant. The bacterial community in the rhizosphere soil of diseased plant was involved in the synthesis of amino acids (glycine, serine, threonine, alanine, aspartate, and glutamate). Results of a redundancy analysis showed that Colletotrichum was negatively correlated with soil available potassium (AK) and the content of soil organic matter (SOC); however, it was positively correlated with soil pH. The relative abundances of Bacillus showed a negative correlation with soil pH and a positive correlation with AK and SOC content. The present study demonstrated that anthracnose could affect the rhizosphere soil microbial communities in common vetch, and soil properties have a close relationship with the rhizosphere soil microbes of common vetch.","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"36 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141738563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous expression of distinct insecticidal genes in potato cultivars encodes resistance against potato tuber moth, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae) 马铃薯栽培品种中不同杀虫基因的异源表达编码了对马铃薯块茎蛾 Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae) 的抗性

IF 2 4区农林科学

Journal of Plant Diseases and Protection Pub Date : 2024-07-14 DOI: 10.1007/s41348-024-00964-4

Muhammad Salim, Allah Bakhsh, Muhammad Nadir Naqqash, Ayhan Gökçe

{"title":"Heterologous expression of distinct insecticidal genes in potato cultivars encodes resistance against potato tuber moth, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae)","authors":"Muhammad Salim, Allah Bakhsh, Muhammad Nadir Naqqash, Ayhan Gökçe","doi":"10.1007/s41348-024-00964-4","DOIUrl":"https://doi.org/10.1007/s41348-024-00964-4","url":null,"abstract":"The potato tuber moth, Phthorimaea operculella (Zeller), is a notorious insect pest of potato incurring substantial yield losses in the field as well as in storage. Chemical control is difficult to exercise due to the latent feeding of the caterpillars and their ability to develop resistance against insecticides. One of the essential components of efficient insect-resistant management is using two or more different insecticidal genes in transgenic crops to effectively avoid and delay the resistance development in insect pests. Two constructs, namely DS-1 (cry3A + SN-19 genes) and DS-2 (OCII + SN-19 genes) in pCAMBIA1301 binary vector, were developed and were transformed in potato cultivars (Agria and Lady Olympia) via Agrobacterium-mediated transformation. The molecular analysis confirmed gene integration and expression of the introduced genes in transgenic plants. The insecticidal effects of incorporated genes in transgenic plants were assessed against 1st, 2nd, 3rd, and 4th instar potato tuber moth (PTM) larvae. The transgenic plants endured significantly high mortalities (100%) of larval stages of PTM within 72 h. Our results show that these transgenic potato plants have the potential to control populations of PTM and are also useful tools in managing PTM that would ultimately reduce the dependency on conventional chemical pesticides with potentially less or minimal hazards. These lines can also serve as an excellent source of germplasm for potato breeding program.","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"17 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141609624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Odourless vegetable oils as insect pest repellents for short-term protection of various food packaging materials 无味植物油作为害虫驱避剂，为各种食品包装材料提供短期保护

IF 2 4区农林科学

Journal of Plant Diseases and Protection Pub Date : 2024-07-08 DOI: 10.1007/s41348-024-00965-3

J. A. Shah, T. Vendl, R. Aulicky, M. Božik, V. Stejskal

{"title":"Odourless vegetable oils as insect pest repellents for short-term protection of various food packaging materials","authors":"J. A. Shah, T. Vendl, R. Aulicky, M. Božik, V. Stejskal","doi":"10.1007/s41348-024-00965-3","DOIUrl":"https://doi.org/10.1007/s41348-024-00965-3","url":null,"abstract":"The repellent activity of two odourless vegetable oils (sweet almond—SAO and soybean—SO) applied on filter paper and four types of food packaging materials (polyethylene film, plastic gunnysacks, cardboard, and wood) was tested against three species of stored product pests (Sitophilus granarius, Tribolium castaneum, and Oryzaephilus surinamensis). The repellence of three doses was evaluated after 24 h, and after 7, 14, 21, and 28 days. Both oils exhibited a repellent effect when applied to filter paper, but this effect decreased with time and concentration. The most susceptible species was T. castaneum. The maximum repellence was 84% and 76% for SO and SAO, respectively. On packaging materials, the repellent effect diminished after a certain period of time, ranging from 7 to 28 days. Subsequently, the oils even became attractive. Generally, the repellent effects of both SO and SAO were higher on cardboard and wood slab than on polyethylene film and plastic gunnysacks. As such, in the case of SO, the repellence against T. castaneum was 88% 24 h after application on cardboard, whilst 68% on plastic bag. This work demonstrated the repellent potential of vegetable oils in the short term for the protection of packaged food against stored product pests.","PeriodicalId":16838,"journal":{"name":"Journal of Plant Diseases and Protection","volume":"52 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141576671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0