Journal of Plant Pathology最新文献_第2页

Distribution of wheat stem rust (Puccinia graminis f. sp. tritici) and its association with biophysical factors and cultivation practices in Horro Guduru Wollega highlands, Western Ethiopia 埃塞俄比亚西部 Horro Guduru Wollega 高地小麦茎锈病（Puccinia graminis f. sp. tritici）的分布及其与生物物理因素和种植方法的关系

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-08-26 DOI: 10.1007/s42161-024-01725-w

Tola Abdissa, Chemeda Fininsa, Bekele Abeyo, Getaneh Woldeab

{"title":"Distribution of wheat stem rust (Puccinia graminis f. sp. tritici) and its association with biophysical factors and cultivation practices in Horro Guduru Wollega highlands, Western Ethiopia","authors":"Tola Abdissa, Chemeda Fininsa, Bekele Abeyo, Getaneh Woldeab","doi":"10.1007/s42161-024-01725-w","DOIUrl":"https://doi.org/10.1007/s42161-024-01725-w","url":null,"abstract":"Wheat stem rust (Puccinia graminis f. sp. tritici) is the major threat to wheat production in Ethiopia due to the menace of high losses in grain yield and quality. A wheat stem rust survey was undertaken during the main cropping seasons (late October to early November) of 2015 and 2016 in the Horro Guduru Wollega highlands, western Ethiopia. The purposes of the survey were to assess the wheat stem rust distribution and disease intensity and determine its association with biophysical factors and cultivation practices. A total of 221 wheat fields in eight districts were assessed. Wheat stem rust was prevalent in both cropping seasons. The mean disease incidence ranged from 56.3 to 63.7%, while the disease severity mean ranged from 15.7 to 21.6%, respectively. Logistic regression was used to analyze the association of wheat stem rust with independent variables. Increased incidence (>50%) and severity (>5%) were strongly (P < 0.0001) associated with lower altitude (≤2400 m), susceptible varieties, broadcast sowing method, fungicide-unsprayed wheat fields, and wheat growth at flowering and milking stages. The survey indicates a highly prevalent wheat stem rust epidemic associated with biophysical factors and cultivation practices in the major wheat-growing districts. A combination of cultivation practices such as sowing wheat in rows, optimum fertilization, deployment of rust-resistant varieties, and effective fungicide application would be the most effective methods for managing wheat stem rust in the Horro Guduru Wollega highlands.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"132 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142192666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genome resource of endophytic Bacillus subtilis GUCC4, a potential biocontrol agent 潜在生物控制剂枯草芽孢杆菌 GUCC4 的完整基因组资源

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-08-26 DOI: 10.1007/s42161-024-01710-3

Zeyang Ding, Hao Ouyang, Cheng Liu, Adeel Abbas, Shijun Mao, Xuqing Yang, Zengliang Liu, Tomislav Cernava, Xiaojing Hu, Xiaoyulong Chen

引用次数: 0

Revisiting Pseudocercospora puerariicola associated with leaf spot on Pueraria montana var. lobata with morphological and molecular-phylogenetic data in Korea 用形态学和分子系统学数据重新审视与韩国葛根叶斑病相关的假丝酵母菌

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-08-23 DOI: 10.1007/s42161-024-01749-2

In-Young Choi, Lamiya Abasova, Ji-Hyun Park, Hyeon-Dong Shin

{"title":"Revisiting Pseudocercospora puerariicola associated with leaf spot on Pueraria montana var. lobata with morphological and molecular-phylogenetic data in Korea","authors":"In-Young Choi, Lamiya Abasova, Ji-Hyun Park, Hyeon-Dong Shin","doi":"10.1007/s42161-024-01749-2","DOIUrl":"https://doi.org/10.1007/s42161-024-01749-2","url":null,"abstract":"Pseudocercospora puerariicola, a leaf spot fungus of Pueraria species, has been reported in East Asian countries, including Korea. Despite the ecological significance of this fungus, to date, no living cultures are present in Korea. The DNA nucleotide sequences of the internal transcribed spacer (ITS) region and the 28S rDNA gene (large subunit, LSU) of the fungus were deposited on GenBank database from Chinese, Laos, and Taiwanese isolates. However, these results were not sufficient to confirm pathogen identification to the species level. Therefore, we aimed to provide further information on this species based on the Korean isolates obtained from Pueraria montana var. lobata, adding more genetic locus to our study to support and affirm the identification of the fungus based on multi-loci analyses from Korean isolates. Nine samples were collected from different areas of Korea and deposited at the Korea University Herbarium, then used in this study. Three monoconidial isolates were obtained in 2022, and molecular phylogenetic analyses were performed using a combined multi-locus dataset of the ITS region, partial actin (actA), partial translation elongation factor 1-alpha (tef-1), and partial DNA-directed RNA polymerase II second largest subunit (rpb2) genes. The present study confirmed the presence of P. puerariicola in Korea as a causal agent of leaf spots on kudzu plants, providing the phylogenetic position of this fungus for the first time based on a multigene dataset and preserving three isolates in an authentic culture collection for future studies.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"2 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142192669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emerging Fig Black Necrosis incited by Butlerelfia eustacei and its potential impact on various fig cultivars Butlerelfia eustacei 引发的新的无花果黑色坏死病及其对各种无花果栽培品种的潜在影响

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-08-20 DOI: 10.1007/s42161-024-01739-4

Hamed Negahban, Zeinab Bolboli, Reza Mostowfizadeh-Ghalamfarsa

{"title":"Emerging Fig Black Necrosis incited by Butlerelfia eustacei and its potential impact on various fig cultivars","authors":"Hamed Negahban, Zeinab Bolboli, Reza Mostowfizadeh-Ghalamfarsa","doi":"10.1007/s42161-024-01739-4","DOIUrl":"https://doi.org/10.1007/s42161-024-01739-4","url":null,"abstract":"The widespread occurrence of fig cankers in the fig orchards of the central regions of Fars Province, Iran, prompted us to investigate the causative agents responsible for this decay. During this study we identified a newly emerged disease in fig trees, which we termed “Fig Black Necrosis”, in the commercially important fig cultivar Sabz. This disease is characterized by black wood necrosis under the bark of the infected fig branches. Our morpho-molecular investigation of fungal isolates from the infected fig trees exhibiting symptoms of black necrosis led to identifying the isolates as the basidiomycete, Butlerelfia eustacei, of the order Atheliales sensu lato. We conducted a comprehensive pathogenicity assessment on detached shoots and one-year-old saplings of eight distinct fig cultivars. The results revealed that B. eustacei could induce conspicuous brown lesions and wood discoloration. Moreover, our study demonstrated that this pathogen could cause wood necrosis and discoloration in detached shoots of 14 distinct tree species associated with various economically significant fruit, landscape, and ornamental trees growing alongside fig trees. In our survey, we clustered the inoculated fig cultivars based on six distinct pathogenicity characteristics using the principal component analysis approach. The results indicated that most fig cultivars, including all dried and most fresh figs, were moderately-susceptible to Fig Black Necrosis. The findings of this study provide insight into the newly emerged disease in fig orchards, aiming to better understand the causal agent and develop effective integrated management strategies against the disease.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"72 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142192670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potency of formulated biocontrol agents from lab to field: a sustainable approach for onion purple blotch suppression 从实验室到田间的配制生物控制剂的效力：抑制洋葱紫斑病的可持续方法

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-08-19 DOI: 10.1007/s42161-024-01744-7

Shubham Saini, Kushal Raj, Rakesh Kumar, Anil Kumar Saini

{"title":"Potency of formulated biocontrol agents from lab to field: a sustainable approach for onion purple blotch suppression","authors":"Shubham Saini, Kushal Raj, Rakesh Kumar, Anil Kumar Saini","doi":"10.1007/s42161-024-01744-7","DOIUrl":"https://doi.org/10.1007/s42161-024-01744-7","url":null,"abstract":"Purple blotch disease incited by Alternaria porri is the major constraint in onion seed production, adversely affecting the quantity and quality of the seed. The indiscriminate and injudicious application of chemical pesticides has led to significant repercussions on both the environment and the overall well-being of living organisms, hence there is an urgent call for a transformative shift towards eco-friendly and sustainable approaches for combating the challenges posed by plant pathogens. In this context, the present study highlights the potency of formulated bio agents in enhancing seed vigour and suppressing onion purple blotch disease. Amongst the four bio agent formulations (Trichoderma harzianum 2.0% WP, Trichoderma viride 1.0% WP, Bacillus subtilis 1.50% LF and Pseudomonas fluorescens 1.75% WP) evaluated for priming impact on onion seedlings, Trichoderma harzianum 2.0% WP exhibited predominance in enhancing seed quality parameters with maximum seedling emergence (73.00 per cent), length (11.24 cm), dry weight (16.02 mg) and vigour indices I (818.63) and II (1168.61). This, in turn, led to maximum enhancement in total soluble sugars, total soluble phenolics, total chlorophyll content, polyphenol oxidase and peroxidase. All the investigated biochemical parameters revealed a positive correlation with vigour indices of seedlings. Trichoderma harzianum 2.0% WP (81.17 per cent) resulted in the maximum reduction in mycelial development of Alternaria porri. The potency of bio formulations under field conditions also exhibited supremacy of Trichoderma harzianum 2.0% WP with maximum disease management (23.08 per cent) representing AUDPC value (623.00), consequently leading to maximum increase in seed yield over control (5.61 per cent). Trichoderma harzianum 2.0% WP could be employed for long-term management of onion purple blotch.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"57 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142192671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increasing virulence and decreasing fungicide sensitivity in Phytophthora capsici after continuous metalaxyl-chlorothalonil exposure 持续接触甲霜灵-百菌清后，疫霉菌的毒力增强，对杀菌剂的敏感性降低

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-08-16 DOI: 10.1007/s42161-024-01713-0

Jacobo Sevillano-Serrano, John Larsen, Fernando Uriel Rojas-Rojas, Julio C. Vega-Arreguín

{"title":"Increasing virulence and decreasing fungicide sensitivity in Phytophthora capsici after continuous metalaxyl-chlorothalonil exposure","authors":"Jacobo Sevillano-Serrano, John Larsen, Fernando Uriel Rojas-Rojas, Julio C. Vega-Arreguín","doi":"10.1007/s42161-024-01713-0","DOIUrl":"https://doi.org/10.1007/s42161-024-01713-0","url":null,"abstract":"Several fungicide products with metalaxyl-chlorothalonil as the active ingredients are available in the Mexican market. Applying products containing this mixture of molecules is a common method that farmers choose to prevent damage caused to crops by Phytophthora capsici. Despite the efficacy of the mixture of metalaxyl-chlorothalonil in controlling the growth of this pathogen, their intensive use could contribute to the development of multidrug-resistant strains. Additionally, the dynamics of resistance to commercial metalaxyl-chlorothalonil-based fungicides and the virulence of P. capsici during continuous exposure are yet to be studied in detail. In this study, four continuous generations of P. capsici were exposed to two concentrations (5 and 100 μg·mL−1) of a commercial fungicide containing metalaxyl-chlorothalonil as the active ingredients. Changes in morphology, growth, and virulence were observed after the first interaction with the fungicide. However, in further generations exposed to the same amount of the product, the morphology and growth of P. capsici were partially restored, whereas its virulence was completely re-established. These results indicate a rapid adaptation to the metalaxyl-chlorothalonil mixture and highlight the importance of using different methods to control P. capsici and prevent the development of more virulent and multidrug-resistant strains.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"24 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142192672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antifungal and plant-growth promoting potency of Trichoderma asperellum against Fusarium wilt on tomato 赤霉菌对番茄镰刀菌枯萎病的抗真菌和促进植物生长作用

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-08-14 DOI: 10.1007/s42161-024-01736-7

A. Selva Amala, V. K. Parthiban, A. Sudha, C. Gopalakrishnan, N. Swarnakumari, R. Anandham

{"title":"Antifungal and plant-growth promoting potency of Trichoderma asperellum against Fusarium wilt on tomato","authors":"A. Selva Amala, V. K. Parthiban, A. Sudha, C. Gopalakrishnan, N. Swarnakumari, R. Anandham","doi":"10.1007/s42161-024-01736-7","DOIUrl":"https://doi.org/10.1007/s42161-024-01736-7","url":null,"abstract":"One of the most well-known vegetables used by the majority of the population worldwide, known as tomato (Solanum lycopersicum), is affected by wilt disease caused by Fusarium oxysporum f. sp. lycopersici (FOL), mainly in the tropics. In the current scenario, a residue-free management approach using a biological control agent is being explored to address the invasion of phytopathogens. This investigation assessed the ability of Trichoderma asperellum (Tv1) to reduce pathogen growth and promote plant growth. A dual culture assay demonstrated that Trichoderma asperellum Tv1 inhibited the growth of the pathogen mycelia by up to 79.63%, and volatile organic compounds (VOCs) emitted by the antagonist inhibited FOL to a maximum of 61.48% of the MGI. An oil-based (OB) formulation was developed using a spore suspension of Tv1 with improved shelf life (3.22 × 108 cfu/ml on the 120th day). Compared with those in the control treatment, significant improvements in vegetative parameters such as germination percentage (100%), root length (8.12 cm) and shoot length (5.58 cm) were observed by the roll towel method upon seed treatment (5 ml/kg) with the OB formulation. There was a minimum wilt disease incidence in tomatoes, up to 19.34% (T8), which was recorded in comparison with that in the inoculated control (T12) (64.75%). Overall, Trichoderma asperellum Tv1 was proven to be an effective biocontrol agent against Fusarium wilt disease in tomato. This study revealed a reduction in mycelial growth of the pathogen caused by mycoparasitism and VOCs under laboratory conditions and wilt disease incidence in a glasshouse study in response to treatment with the biocontrol agent Tv1. With this viewpoint, this approach could be considered a promising environmentally safe approach for managing wilt disease in tomatoes.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"4 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142192675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and pathogenicity of fungal species associated with lettuce wilt and root rot in Vietnam 越南莴苣枯萎病和根腐病相关真菌种类的多样性和致病性

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-08-14 DOI: 10.1007/s42161-024-01740-x

Dung Le, Loan Thi Minh Tran, Lan Thi Cao, Tuan Ngoc Pham, Anh Quang Dinh, Uyen Khanh Nguyen

{"title":"Diversity and pathogenicity of fungal species associated with lettuce wilt and root rot in Vietnam","authors":"Dung Le, Loan Thi Minh Tran, Lan Thi Cao, Tuan Ngoc Pham, Anh Quang Dinh, Uyen Khanh Nguyen","doi":"10.1007/s42161-024-01740-x","DOIUrl":"https://doi.org/10.1007/s42161-024-01740-x","url":null,"abstract":"Since the beginning of 2023, an outbreak of Fusarium wilt-like symptoms on lettuce (Lactuca sativa L.) has been observed in Lam Dong province, Vietnam. Wilting consistently combined with yellow leaves, root rot and stunted growth are common symptoms of infected plants. Thirty-one fungal isolates were recovered from symptomatic lettuce plants and identified based on morphological and molecular characteristics (rDNA internal transcribed spacer (ITS) and the translation elongation factor (TEF-1α) gene sequences) as F. oxysporum (51.6%), F. incarnatum-equiseti species complex [FIESC: F. compactum (16.1%) and F. sulawesiense (3.2%)], and Plectosphaerella cucumerina (29.0%). The composition of these fungi varied between locations, with co-infection by P. cucumerina and Fusarium spp. observed in lettuce plants from the same field. Pathogenicity tests showed that all isolates were pathogenic to lettuce, causing reduced seed germination, induced collar rot, and decreased seedling survival and mass. Notably, F. oxysporum isolates caused collar rot earlier, significantly reducing seedling survival and indicating faster disease progression than P. cucumerina and FIESC. Additionally, there was wide variation in virulence and specialization of isolates at different stages of seedling growth: P. cucumerina L0311 primarily reduced seed germination, while F. oxysporum L0911 and L0331 strongly inhibited seedling growth. These findings suggest a shift in the pathogenic roles of the isolates during seed germination and seedling growth, with potential variation continuing post-nursery. This work is the first to report on the population and virulence profile of fungi causing lettuce wilt in Vietnam, providing essential insights for quarantine measures, disease monitoring, and management strategies.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"7 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142192674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing a differential system for designating the pathotypes of Puccinia hordei causing barley leaf rust in India 开发一套用于指定印度导致大麦叶锈病的 Puccinia hordei 病原型的差异系统

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-08-13 DOI: 10.1007/s42161-024-01734-9

Subodh Kumar, S. C. Bhardwaj, O. P. Gangwar, P. Prasad, Charu Lata, Ajit Singh, A. S. Manjul

{"title":"Developing a differential system for designating the pathotypes of Puccinia hordei causing barley leaf rust in India","authors":"Subodh Kumar, S. C. Bhardwaj, O. P. Gangwar, P. Prasad, Charu Lata, Ajit Singh, A. S. Manjul","doi":"10.1007/s42161-024-01734-9","DOIUrl":"https://doi.org/10.1007/s42161-024-01734-9","url":null,"abstract":"The virulence spectrum of leaf rust of barley (Puccinia hordei Otth.) has not been studied in India so far. This is a first study to design a system of pathotype nomenclature and know the virulence structure of pathotypes of P. hordei in India. All the available near-isogenic lines (NILs) of Rph genes, lines with known Rph genes, and other barley accessions including promising Indian barley cultivars were screened using 328 P. hordei isolates from different parts of India, Bhutan, and Nepal. Using a binomial system of nomenclature, 11 distinct pathotypes of P. hordei occurring in India were identified and named. A grouping of pathotypes in the 328 leaf rust samples analyzed since 1999, it was evident that pathotype H2 followed by H3, H7, H4 and H1 were the most predominant pathotypes. Based on the molecular studies, all the pathotypes were distinct and fell into three groups (A, B, and C). Group A further clustered into two subgroups A1 (4 pathotypes) and A2 (2 pathotypes). The virulence phenotype and molecular genotype based distinction of P. hordei pathotypes will help in the precise screening of barley germplasm and the identification of rust-resistant lines. The establishment of an Indian differential system for the designation of P. hordei isolates will also help in monitoring the shift in virulence patterns, predominance, and emerging of new pathotypes in the future and will help scientists of other countries to make comparisons with their virulences.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"82 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142192714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete genome sequencing and infectious cDNA clone construction of a Chinese isolate of grapevine Pinot gris virus (GPGV) 葡萄灰比诺病毒（GPGV）中国分离株的全基因组测序和感染性 cDNA 克隆构建

IF 2.2 4区农林科学

Journal of Plant Pathology Pub Date : 2024-08-13 DOI: 10.1007/s42161-024-01737-6

Qingyun Yuan, Ying Zhang, Fang Ren, Guojun Hu, Xudong Fan, Yafeng Dong

{"title":"Complete genome sequencing and infectious cDNA clone construction of a Chinese isolate of grapevine Pinot gris virus (GPGV)","authors":"Qingyun Yuan, Ying Zhang, Fang Ren, Guojun Hu, Xudong Fan, Yafeng Dong","doi":"10.1007/s42161-024-01737-6","DOIUrl":"https://doi.org/10.1007/s42161-024-01737-6","url":null,"abstract":"The complete nucleotide sequence of a Chinese isolate C12B1 of grapevine Pinot gris virus (GPGV) from a ‘Beta’ grapevine was determined by small RNA sequencing. The size of the C12B1 genome was 7259 nucleotides (nt) without the poly (A) tail. Sequence identities of C12B1 with other GPGV isolates ranged from 79.13 to 97.52%. Phylogenetic analysis based on complete genome sequences of the global GPGV isolates indicated GPGV isolates are divided into three major groups and five subgroups within group 3, with the C12B1 isolate felled within subgroup 3a of group 3. Moreover, an infectious cDNA clone of the GPGV isolate C12B1 was generated using the seamless assembly approach. The infectious clone was then inoculated to Nicotiana occidentalis 37B and grapevine plants. Inoculated Nicotiana plants showed leaf chlorotic mottling symptoms and RT-PCR and transmission electron microscopy analysis confirmed successful infection. Additionally, the inoculated Beta grapevine plants also exhibited mild chlorotic mottling and ring spot symptoms. This study represents the first complete genome sequence of GPGV in China and the successful production of an infectious cDNA clone of a divergent GPGV isolate. These findings are a foundation for further research on the interaction between GPGV and its plant hosts.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"9 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142192715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0