Journal of Plant Pathology最新文献

{"title":"Isolation and identification of endophytic and grapevine trunk diseases associated fungi with antagonistic potential against Cytospora chrysosperma","authors":"Sahar Pazooki, Azam Shekariesfahlan, Mojdeh Maleki, Shahram Naeimi","doi":"10.1007/s42161-024-01731-y","DOIUrl":"https://doi.org/10.1007/s42161-024-01731-y","url":null,"abstract":"<p>Grapevine is one of the most important fruit trees which has been used by human since ancient times and is considered as a major agricultural product in Iran. However, <i>Vitis vinifera</i> (the cultivated species) is susceptible to many fungal pathogens which can lead to economic losses or decline of the vineyards. Among them, grapevine trunk diseases (GTDs) are problematical and associated with many different fungi, such as <i>Cytospora chrysosperma</i>. 293 endophytic and GTDs-associated fungi were isolated from 7 provinces of Iran. Based on morphological characteristics, 25 representative isolates were selected and identified using internal transcribed spacer (ITS-rDNA) region. They belonged to 14 genera of <i>Aspergillus</i>, <i>Cephalotrichum</i>,<i> Chaetomium</i>,<i> Coniochatea</i>,<i> Fusarium</i>,<i> Microascus</i>,<i> Neosetophoma</i>,<i> Penicillium</i>,<i> Phaeoacremonium</i>,<i> Phialemonium</i>,<i> Preussia</i>,<i> Stromatinia</i>,<i> Tricharina</i>, and <i>Truncatella</i>. Using the dual culture method, the antagonistic activity of 13 isolates from those 25 selected ones was shown against <i>Cytospora chrysosperma</i>; in which its hyphal growth was inhibited from 10 to 41.33%. Among tested fungi, <i>Fusarium</i> sp3.-GI154, <i>Fusarium</i> sp4.-GI267, <i>Fusarium</i> sp5.-GI289, <i>Penicillium canescens</i>-GI101 and <i>Chaetomium</i> sp.-GI179 showed the highest antifungal activity, respectively. Peptide exctract of <i>Fusarium</i> sp.3-GI154 displayed inhibitory activity against <i>Cytospora chrysosperma</i>. To the best of our knowledge, this is the first report of <i>Aspergillus europaeus</i>, <i>Cephalotrichum verucisporum</i>, <i>Microascus chartarus</i>, <i>Neosetophoma salicis</i>, <i>Penicillium canescens</i>, <i>Penicillium expansum</i>, <i>Preussia minima</i> and <i>Tricharina</i> sp. as endophytes and <i>Phialemonium</i> sp. as a GTDs-associated fungus from grapevine in Iran. Endophytes can provide an interesting field for searching appropriate approaches of grapevine diseases biological control.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141968752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological responses and differential expression of genes involved in ABA and SA signaling during the interaction of the carnation (Dianthus caryophyllus L.) and the fungus Fusarium oxysporum f. sp. dianthi","authors":"E. Bustos-Caro, L.M. Melgarejo, A.M. Pinzón, H.D. Ardila","doi":"10.1007/s42161-024-01687-z","DOIUrl":"https://doi.org/10.1007/s42161-024-01687-z","url":null,"abstract":"<p>The carnation (<i>Dianthus caryophyllus</i> L.) stands as a key export important for Colombia, yet its production faces challenges due to vascular wilting triggered by <i>Fusarium oxysporum</i> f. sp. <i>dianthi</i> (Fod). This study examined Fod impact on stomatal resistance, leaf temperature, and dry biomass in carnation plants, alongside its influence on rooted cuttings of two carnation cultivars differing in susceptibility to vascular wilting. Employing a completely randomized design, the resistant cultivar ‘Golem’ and susceptible cultivar ‘Mizuki’, each with four weeks of rooting, were inoculated with Fod, while controls were maintained. Both cultivars exhibited altered leaf temperatures and AUDPC (Area Under Disease Progression Curve) parameters post-inoculation, compared to their controls, indicating leaf temperature’s potential as an early Fod presence indicator. The effects of inoculation with Fod for each cultivar were different for stomatal resistance, associated with the asymptomatic stage of the disease. These findings suggest that the resistant cultivar activates early stomatal closure mechanisms, possibly through hormonal pathways, enhancing its ability to combat the pathogen. Real-time PCR analysis of <i>rga1</i> and <i>mlp43-like</i> genes indicated the involvement of Abscisic Acid (ABA) and Salicylic Acid (SA) hormonal pathways in cultivar-specific responses post-inoculation, even at early stages. These results highlighted Fod presence before symptom development and their significance for devising early detection strategies and breeding genetically resistant cultivars. This study underscores the intricate interplay between plant-pathogen interactions and hormonal signaling pathways, providing valuable insights for effective disease management and cultivar development in carnation production.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141882192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Ralstonia solanacearum RipAX family effectors repress the phosphorylation of host MAPKs","authors":"Zhen Meng, Xiaoying Zheng, Jia Zhang, Yulan Ruan, Yanhui Wu, Mei Luo, Lina Xiong, Handa Song, Guohui Yu, Zhangyong Dong, Yunhao Sun","doi":"10.1007/s42161-024-01727-8","DOIUrl":"https://doi.org/10.1007/s42161-024-01727-8","url":null,"abstract":"<p>Bacterial wilt caused by strains of the <i>Ralstonia solanacearum</i> species complex is a severe disease affecting plants. The type III secretion system delivers type III effectors (T3Es) from the pathogen to the host plant and is one of the main virulence determinants of <i>R. solanacearum</i> strains. However, the functions of most T3Es, including the <i>R. solanacearum</i> effectors RipAX1 and RipAX2, are not completely understood. In this study, we determined that RipAX1 and RipAX2 share a potential but highly conserved M91 metalloenzyme structural domain. Heterologous overexpression of <i>RipAX1</i> or <i>RipAX2</i> neither caused nor inhibited the hypersensitive response (HR) of plant tissues, and their expression in <i>Saccharomyces cerevisiae</i> did not inhibit yeast growth. RipAX1 and RipAX2 localized to the cytoplasm of <i>Nicotiana benthamiana</i> leaf epidermal cells and <i>Arabidopsis thaliana</i> protoplasts. The two effectors interacted with specific members of the plant mitogen-activated protein kinase (MAPK) family. However, RipAX1 and RipAX2 did not directly cleave MAPKs, despite their predicted metalloenzyme activity, although RipAX1 inhibited the phosphorylation of plant MAPKs. These results elucidate a novel aspect of the molecular mechanism underlying the repression of host immunity by T3Es from <i>R. solanacearum</i> species complex strains.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141882190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete genome sequence of Erwinia pyrifoliae strain YKB12327, isolated from an apple tree in Korea","authors":"Hualin Nie, Sanghee Lee, Seo-Rin Ko, Young Kee Lee, Mi-Hyun Lee, Jun Myoung Yu, Jae Sun Moon, Ah-Young Shin, Suk-Yoon Kwon","doi":"10.1007/s42161-024-01708-x","DOIUrl":"https://doi.org/10.1007/s42161-024-01708-x","url":null,"abstract":"<p><i>Erwinia pyrifoliae</i>, the Gram-negative bacterial pathogen responsible for black shoot blight, exhibits symptoms similar to <i>E. amylovora</i> (fire blight), though with distinct molecular characteristics. Given its prevalence primarily in South Korea and the availability of only nine assembled genomes, there is a lack of high-quality genome sequences and annotated genetic information for <i>E. pyrifoliae</i>. We present the sequencing and assembly of a Korean <i>E. pyrifoliae</i> strain, YKB12327, isolated from a diseased apple tree branch, using a combination of long Oxford Nanopore Technologies and short Illumina sequence reads. This genome comprises a circular chromosome and three plasmid sequences, totaling 4,061,634 bp. Annotation of YKB12327 identifies 3123 coding sequence protein-coding genes, 22 rRNA genes (5S, 16S, and 23S), and 76 tRNA genes. Our sequence data will enrich the current <i>E. pyrifoliae</i> genome resources and facilitate in understanding its evolution, diversity and structural variations, as well as the molecular basis of pathogenesis.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141869274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistance to downy mildew in wildly growing Eurasian Vitis vinifera L. grapevines","authors":"N. Bitsadze, Sh. Kikilashvili, R. Chipashvili, L. Mamasakhlisashvili, T. Maghradze, M. Kikvadze, S. L. Toffolatti, G. De Lorenzis, O. Failla, R. Ocete Rubio, D. Maghradze","doi":"10.1007/s42161-024-01728-7","DOIUrl":"https://doi.org/10.1007/s42161-024-01728-7","url":null,"abstract":"<p><i>Plasmopara viticola</i> (Berk. &amp; M. A. Curtis) Berl. &amp; De Toni, the causal agent of downy mildew, affects both cultivated and wild grapevine. Wild species of the genus <i>Vitis</i> L. are reliable sources of resistance to many of the diseases that affect cultivated grapevines. For this reason, it is important to study wildly growing (typical wild and feral) accessions belonging to <i>V. vinifera</i> L. The aim of this research was to study the levels of resistance to <i>P. viticola</i> of 57 Georgian wildly growing grapevine accessions by leaf disc method. The data were collected over eight years, from 2014 to 2021. Different degrees of resistance have been detected, ranging from susceptible (8.8% of accessions), low resistance (8.8% of accessions) moderate resistance (22.8%), and resistance (12.3% of accessions). Resistance of the 33,3% of the tested accessions was unstable and varied from susceptible to resistance across the years. Among resistant wildly growing grapevine genotypes, there are several accessions with a high density of the trichomes, although the data showed that the density of prostrate and erect hairs of the leaves is not correlated with the resistance trait. A large number of resistant genotypes among wildly growing grapevines suggests that in its natural habitat more resistant forms to downy mildew survives in the process of natural selection. Further studies are needed to characterize the plant-pathogen interaction and the mechanism of resistance of the wild accessions, although the resistant accessions, such as Tedotsminda 07, Tedotsminda 09, Tedotsimda 10 can be interesting for the breeding purposes in the future.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141869272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Plasmopara viticola isolate surpasses grapevine Rpv3.1 and Rpv3.2 resistance but not Rpv12","authors":"Catarina Gouveia, Rita B. Santos, Selma Zukic, Thorsten Manthey, Rui Malhó, Andreia Figueiredo, Guenther Buchholz","doi":"10.1007/s42161-024-01707-y","DOIUrl":"https://doi.org/10.1007/s42161-024-01707-y","url":null,"abstract":"<p>Grape downy mildew, caused by the oomycete <i>Plasmopara viticola</i>, is a major threat to <i>Vitis vinifera</i> grape production. Several breeding programs rely on resistance factors (<i>Rpv</i>s) from American and Asian <i>Vitis</i> species to confer resistance to <i>P. viticola</i>. Although the use of these crossing hybrids offers a promising strategy for disease control, variants capable of overcoming host resistance are emerging. A thorough characterization of these isolates is needed to define new strategies for breeding programs. The investigation of varying aggressiveness of two <i>P. viticola</i> pathotypes was investigated. One pathotype is unable to overcome <i>Rpv3.1</i> based resistance and another pathotype was isolated from a grapevine carrying the <i>Rpv3.1</i> locus. Their aggressiveness was evaluated on five grapevine genotypes harboring distinct combinations of Rpv loci. Macroscopic and microscopic development of the pathogen was characterised throughout its colonization of grapevine leaves. Our findings demonstrate that the <i>P. viticola</i> isolate NW-10/16 is not only capable of overcoming <i>Rpv3</i>-based resistance but also exhibited a faster growth, increased sporulation, and a denser mycelial colonization within the plant mesophyll when compared with the avRpv3 + isolate in susceptible plants. The identification and characterization of new, more aggressive, <i>P. viticola</i> pathotypes is crucial to promote durable management of the resistant grapevine hybrids and to mitigate the threat of downy mildew.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141869315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome sequence analysis of two recombinant isolates of watermelon mosaic virus from Iran and Iraq","authors":"Hayder R. Alinizi, Zohreh Moradi, Mohsen Mehrvar","doi":"10.1007/s42161-024-01724-x","DOIUrl":"https://doi.org/10.1007/s42161-024-01724-x","url":null,"abstract":"<p>The pathogenic watermelon mosaic virus (WMV) causes severe crop losses and threatens cucurbits production worldwide. This study presents the first sequenced genome of WMV from Iraq and the second from Iran. The near-complete genome sequences of Iranian (In) and Iraqi (Iq) isolates were, respectively, 9999 and 10,041 nucleotides in length, excluding the 3′-terminal poly(A) tail. WMV-Iq and WMV-In shared nucleotide identities of 92.61–96.32% with those of 30 worldwide representative WMV isolates in GenBank and identities of 93.04–93.90% with a previously reported Iranian isolate IR02-54. Phylogenetic analysis based on the genomic sequences clustered all 32 isolates into seven groups (G1–G7), in which WMV-Iq and WMV-In clustered alongside 12 isolates from Asia, Europe, and America in group G1. Phylogenetic analysis of nucleotide sequences of the coat protein gene of WMV isolates resolved into five phylogroups (I–V) and both isolates, WMV-Iq and WMV-In, clustered together with isolates in group I and contain the N-terminal KEA motif, which is the characteristic of group I isolates. Our results show that WMV-Iq and WMV-In are recombinant isolates with multiple recombination events detected across the genome. Symptom intensity of WMV isolates in experimental hosts was variable and depended upon the host species, virulence of isolates, and environmental conditions.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141778250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of anthracnose on Paeonia delavayi caused by Colletotrichum siamense in China","authors":"Yue Pan, G. Yang, Jianfu Liu, Yulan Wu, Liuwei Xu, Tai Yin, Juan Wang","doi":"10.1007/s42161-024-01726-9","DOIUrl":"https://doi.org/10.1007/s42161-024-01726-9","url":null,"abstract":"","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141803836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of anthracnose caused by Colletotrichum tabaci on Houttuynia cordata in China","authors":"Yuxin Zhao, Yaru Li, Keyun Zhang, Hailin Xia, C. Cui","doi":"10.1007/s42161-024-01716-x","DOIUrl":"https://doi.org/10.1007/s42161-024-01716-x","url":null,"abstract":"","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141811040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of Paecilomyces maximus causing dieback on cherry in Türkiye","authors":"Gülten Nisan Ozan, Deniz Çaplık, H. Bayraktar","doi":"10.1007/s42161-024-01717-w","DOIUrl":"https://doi.org/10.1007/s42161-024-01717-w","url":null,"abstract":"","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141812787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}