Journal of Plant Pathology最新文献

{"title":"Morphological and biochemical changes in asymptomatic and moderately symptomatic plants infected with sugarcane white leaf (SCWL) phytoplasma","authors":"Pimpilai Saengmanee, Parichart Burns, Jutatape Watcharachaiyakup, Udomsak Lertsuchatavanich, Praderm Wanichananan, Supattana Chanta, Sutticha Na-Ranong Thammasittirong, Sonthichai Chanpreme","doi":"10.1007/s42161-024-01729-6","DOIUrl":"https://doi.org/10.1007/s42161-024-01729-6","url":null,"abstract":"<p>Sugarcane white leaf disease associated with sugarcane white leaf (SCWL) phytoplasma is an important disease in sugarcane resulting in significant losses in production in Asian countries including the major regional producing countries of India and Thailand. In this study the morphological and biochemical traits in SCWL phytoplasma infected sugarcane plants, both asymptomatic (AS) and symptomatic (S1-S3) and their corresponding to phytoplasma loads, were investigated. This study found that as symptom development progressed, cane statues including plant height, leaf length, and leaf width, were reduced and corresponded with declines in both chlorophyll and dietary fiber content. Total soluble solids (TSS) were less in AS cane plants, being 16.83 ± 1.0 °brix compared to 20.58 ± 1.6 °brix in healthy plants, declining further with the progression of symptoms. This feature could be potentially used in the identification of AS sugarcanes. Phytoplasma titres increased from 2.36E + 05 copies/µg plant DNA in AS infected leaves, peaking at 1.20E + 08 copies/µg plant DNA in S3 infected leaves, before declining in severely (S4) infected leaves. AS infected plants also exhibited a peak in total phenolic compounds. By tracking the development of symptoms in sugarcane white leaf disease and the corresponding increases in titres of SCWL phytoplasma, decline in chlorophyll and dietary fiber, decrease in TSS, and a peak in total phenolic compounds at the asymptomatic stage, this study confirmed that SCWL phytoplasma significantly impacts the sugarcane host plants as early as the AS stage, this stage is potentially the focus point for early detection and interventions for controlling proliferation SCWL disease.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"57 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142192759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cowpea leaf and pod spots caused by Periconia igniaria and their potential control using biosynthesized zinc oxide and magnesium oxide nanoparticles","authors":"Faten H. Y. Abdel-Samad, Qi Huang, Marzouk R. Abdel-Latif, Zekry A. Shehata, Hanaa A. H. Armanyous, Abdelmonim Ali Ahmad","doi":"10.1007/s42161-024-01714-z","DOIUrl":"https://doi.org/10.1007/s42161-024-01714-z","url":null,"abstract":"<p>Cowpea is subject to attacks by a wide range of plant pathogens including bacteria, fungi, viruses, and nematodes. In this study, a field survey was conducted in cowpea growing regions of El-Minya Governorate of Egypt including Minya, Beni Mazar and Maghagha districts for leaf and pod spot disease. Our results revealed that the disease was present in all surveyed fields with the highest disease incidence and severity found in Minya District. For the first time in Egypt, the causal agent of the disease was determined to be <i>Periconia igniaria</i> based on morphology of the fungal isolates, internal transcribed spacer sequence homology to a <i>P. igniaria</i> strain, and fulfillment of Koch’s postulates. To explore control measures, zinc oxide (ZnO) and magnesium oxide (MgO) nanoparticles (NPs) were synthesized biologically using green coffee extract. Both NPs were characterized, and their formulations confirmed using scanning electron microscopy and the energy spectrum dispersion analysis. The biosynthesized ZnO- and MgO-NPs were demonstrated to have antifungal activity against in vitro mycelium growth of <i>P</i>. <i>igniaria</i> at all tested concentrations from 25 to 200 ppm, and ZnO NPs were more effective than MgO NPs at the same concentration. When 100 ppm of ZnO NPs was tested <i>in planta</i>, it significantly reduced disease incidence and severity in detached cowpea leaves and pods under laboratory conditions, and in cowpea plants under greenhouse conditions. Our results demonstrated that the biosynthesized ZnO NPs have great potential to be developed into an effective and eco-friendly control method against cowpea disease caused by <i>P. igniaria.</i></p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"63 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141968751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of post-harvest quality of Balady lime fruit with Aloe vera gel and tea tree oil against green mold disease caused by Penicillium digitatum","authors":"Eman A. A. Abou-Zaid, Azza S. Hussein, Raoof Sultan, Kamal A. M. Abo-Elyousr, Nashwa M. A. Sallam, Hadeel M. M. Khalil Bagy","doi":"10.1007/s42161-024-01705-0","DOIUrl":"https://doi.org/10.1007/s42161-024-01705-0","url":null,"abstract":"<p><i>Penicillium digitatum</i> Sacc is a common postharvest pathogen affecting lime fruits. It causes significant losses in postharvest lime production. An emerging alternative to chemical control is the use of environmentally friendly such as edible and biodegradable films and packaging. The goal of the current study was to test different concentrations of <i>Aloe vera</i> gel (5, 15, 25, 50, 75, and 100% v/v), tea tree oil coating (0.5, 1, 2.5, 5, and 10% v/v), and their combination for controlling <i>P. digitatum in vitro.</i> The results revealed that specific concentrations of <i>Aloe vera</i> gel (75% and 100% v/v), tea tree oil (2.5, 5, and 10% v/v), and <i>Aloe vera</i> gel (25%) + tea tree oil (1%) v/v showed the highest inhibitory effect against the pathogen. The most effective concentrations of <i>Aloe vera</i> gel (75%), tea tree oil (2.5%), and <i>Aloe vera</i> gel (25%) + tea tree oil (1%) were evaluated against the green mold disease of lime under natural infection through storage period (0, 7, 14, 28 days). Compared to the control group, all edible layers demonstrated a significant delay and reduced in disease severity percentage throughout the storage periods. However, coating lime fruits with a combination of <i>Aloe vera</i> gel (25%) + tea tree oil (1%) successfully prevented the green mold disease of lime fruits over 28 days. The impact of these coatings was evaluated on lime fruit quality maintenance (weight loss, firmness, juice weight, total soluble solids, total acidity, pH, vitamin C, lipid peroxidation content, total phenols, total flavonoid, and total protein) over 28 days of storage during the 2021 and 2022 seasons. All treatments improved fruit quality through the storage period compared with untreated fruits. Treated lime fruits with <i>Aloe vera</i> gel (25%) + tea tree oil (1%) exhibited slight reductions in weight loss and juice weight compared to alternative treatments as an average of the two successive seasons. Simultaneously, this treatment improved fruit firmness, vitamin C levels, total phenols, total flavonoid, and total protein when contrasted with other treatments as an average of the two studied seasons. These findings show that the combination of <i>Aloe vera</i> gel and tea tree oil coatings enhances the shelf life and maintains the quality of lime fruits. They can also be used as an environmentally acceptable, chemical-free alternative treatment to preserve the postharvest quality of lime fruit.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"2 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141931351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete genome coding sequence of freesia sneak virus from Freesia refracta necrotic leaves","authors":"Silvia Rotunno, Monica Marra, Fulco Frascati, Roberto Pierro, Pasquale Restuccia, John Hammond, Laura Miozzi, Anna Maria Vaira","doi":"10.1007/s42161-024-01712-1","DOIUrl":"https://doi.org/10.1007/s42161-024-01712-1","url":null,"abstract":"","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"74 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141968753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and identification of endophytic and grapevine trunk diseases associated fungi with antagonistic potential against Cytospora chrysosperma","authors":"Sahar Pazooki, Azam Shekariesfahlan, Mojdeh Maleki, Shahram Naeimi","doi":"10.1007/s42161-024-01731-y","DOIUrl":"https://doi.org/10.1007/s42161-024-01731-y","url":null,"abstract":"<p>Grapevine is one of the most important fruit trees which has been used by human since ancient times and is considered as a major agricultural product in Iran. However, <i>Vitis vinifera</i> (the cultivated species) is susceptible to many fungal pathogens which can lead to economic losses or decline of the vineyards. Among them, grapevine trunk diseases (GTDs) are problematical and associated with many different fungi, such as <i>Cytospora chrysosperma</i>. 293 endophytic and GTDs-associated fungi were isolated from 7 provinces of Iran. Based on morphological characteristics, 25 representative isolates were selected and identified using internal transcribed spacer (ITS-rDNA) region. They belonged to 14 genera of <i>Aspergillus</i>, <i>Cephalotrichum</i>,<i> Chaetomium</i>,<i> Coniochatea</i>,<i> Fusarium</i>,<i> Microascus</i>,<i> Neosetophoma</i>,<i> Penicillium</i>,<i> Phaeoacremonium</i>,<i> Phialemonium</i>,<i> Preussia</i>,<i> Stromatinia</i>,<i> Tricharina</i>, and <i>Truncatella</i>. Using the dual culture method, the antagonistic activity of 13 isolates from those 25 selected ones was shown against <i>Cytospora chrysosperma</i>; in which its hyphal growth was inhibited from 10 to 41.33%. Among tested fungi, <i>Fusarium</i> sp3.-GI154, <i>Fusarium</i> sp4.-GI267, <i>Fusarium</i> sp5.-GI289, <i>Penicillium canescens</i>-GI101 and <i>Chaetomium</i> sp.-GI179 showed the highest antifungal activity, respectively. Peptide exctract of <i>Fusarium</i> sp.3-GI154 displayed inhibitory activity against <i>Cytospora chrysosperma</i>. To the best of our knowledge, this is the first report of <i>Aspergillus europaeus</i>, <i>Cephalotrichum verucisporum</i>, <i>Microascus chartarus</i>, <i>Neosetophoma salicis</i>, <i>Penicillium canescens</i>, <i>Penicillium expansum</i>, <i>Preussia minima</i> and <i>Tricharina</i> sp. as endophytes and <i>Phialemonium</i> sp. as a GTDs-associated fungus from grapevine in Iran. Endophytes can provide an interesting field for searching appropriate approaches of grapevine diseases biological control.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"4 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141968752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological responses and differential expression of genes involved in ABA and SA signaling during the interaction of the carnation (Dianthus caryophyllus L.) and the fungus Fusarium oxysporum f. sp. dianthi","authors":"E. Bustos-Caro, L.M. Melgarejo, A.M. Pinzón, H.D. Ardila","doi":"10.1007/s42161-024-01687-z","DOIUrl":"https://doi.org/10.1007/s42161-024-01687-z","url":null,"abstract":"<p>The carnation (<i>Dianthus caryophyllus</i> L.) stands as a key export important for Colombia, yet its production faces challenges due to vascular wilting triggered by <i>Fusarium oxysporum</i> f. sp. <i>dianthi</i> (Fod). This study examined Fod impact on stomatal resistance, leaf temperature, and dry biomass in carnation plants, alongside its influence on rooted cuttings of two carnation cultivars differing in susceptibility to vascular wilting. Employing a completely randomized design, the resistant cultivar ‘Golem’ and susceptible cultivar ‘Mizuki’, each with four weeks of rooting, were inoculated with Fod, while controls were maintained. Both cultivars exhibited altered leaf temperatures and AUDPC (Area Under Disease Progression Curve) parameters post-inoculation, compared to their controls, indicating leaf temperature’s potential as an early Fod presence indicator. The effects of inoculation with Fod for each cultivar were different for stomatal resistance, associated with the asymptomatic stage of the disease. These findings suggest that the resistant cultivar activates early stomatal closure mechanisms, possibly through hormonal pathways, enhancing its ability to combat the pathogen. Real-time PCR analysis of <i>rga1</i> and <i>mlp43-like</i> genes indicated the involvement of Abscisic Acid (ABA) and Salicylic Acid (SA) hormonal pathways in cultivar-specific responses post-inoculation, even at early stages. These results highlighted Fod presence before symptom development and their significance for devising early detection strategies and breeding genetically resistant cultivars. This study underscores the intricate interplay between plant-pathogen interactions and hormonal signaling pathways, providing valuable insights for effective disease management and cultivar development in carnation production.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"50 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141882192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Ralstonia solanacearum RipAX family effectors repress the phosphorylation of host MAPKs","authors":"Zhen Meng, Xiaoying Zheng, Jia Zhang, Yulan Ruan, Yanhui Wu, Mei Luo, Lina Xiong, Handa Song, Guohui Yu, Zhangyong Dong, Yunhao Sun","doi":"10.1007/s42161-024-01727-8","DOIUrl":"https://doi.org/10.1007/s42161-024-01727-8","url":null,"abstract":"<p>Bacterial wilt caused by strains of the <i>Ralstonia solanacearum</i> species complex is a severe disease affecting plants. The type III secretion system delivers type III effectors (T3Es) from the pathogen to the host plant and is one of the main virulence determinants of <i>R. solanacearum</i> strains. However, the functions of most T3Es, including the <i>R. solanacearum</i> effectors RipAX1 and RipAX2, are not completely understood. In this study, we determined that RipAX1 and RipAX2 share a potential but highly conserved M91 metalloenzyme structural domain. Heterologous overexpression of <i>RipAX1</i> or <i>RipAX2</i> neither caused nor inhibited the hypersensitive response (HR) of plant tissues, and their expression in <i>Saccharomyces cerevisiae</i> did not inhibit yeast growth. RipAX1 and RipAX2 localized to the cytoplasm of <i>Nicotiana benthamiana</i> leaf epidermal cells and <i>Arabidopsis thaliana</i> protoplasts. The two effectors interacted with specific members of the plant mitogen-activated protein kinase (MAPK) family. However, RipAX1 and RipAX2 did not directly cleave MAPKs, despite their predicted metalloenzyme activity, although RipAX1 inhibited the phosphorylation of plant MAPKs. These results elucidate a novel aspect of the molecular mechanism underlying the repression of host immunity by T3Es from <i>R. solanacearum</i> species complex strains.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"7 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141882190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete genome sequence of Erwinia pyrifoliae strain YKB12327, isolated from an apple tree in Korea","authors":"Hualin Nie, Sanghee Lee, Seo-Rin Ko, Young Kee Lee, Mi-Hyun Lee, Jun Myoung Yu, Jae Sun Moon, Ah-Young Shin, Suk-Yoon Kwon","doi":"10.1007/s42161-024-01708-x","DOIUrl":"https://doi.org/10.1007/s42161-024-01708-x","url":null,"abstract":"<p><i>Erwinia pyrifoliae</i>, the Gram-negative bacterial pathogen responsible for black shoot blight, exhibits symptoms similar to <i>E. amylovora</i> (fire blight), though with distinct molecular characteristics. Given its prevalence primarily in South Korea and the availability of only nine assembled genomes, there is a lack of high-quality genome sequences and annotated genetic information for <i>E. pyrifoliae</i>. We present the sequencing and assembly of a Korean <i>E. pyrifoliae</i> strain, YKB12327, isolated from a diseased apple tree branch, using a combination of long Oxford Nanopore Technologies and short Illumina sequence reads. This genome comprises a circular chromosome and three plasmid sequences, totaling 4,061,634 bp. Annotation of YKB12327 identifies 3123 coding sequence protein-coding genes, 22 rRNA genes (5S, 16S, and 23S), and 76 tRNA genes. Our sequence data will enrich the current <i>E. pyrifoliae</i> genome resources and facilitate in understanding its evolution, diversity and structural variations, as well as the molecular basis of pathogenesis.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"160 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141869274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resistance to downy mildew in wildly growing Eurasian Vitis vinifera L. grapevines","authors":"N. Bitsadze, Sh. Kikilashvili, R. Chipashvili, L. Mamasakhlisashvili, T. Maghradze, M. Kikvadze, S. L. Toffolatti, G. De Lorenzis, O. Failla, R. Ocete Rubio, D. Maghradze","doi":"10.1007/s42161-024-01728-7","DOIUrl":"https://doi.org/10.1007/s42161-024-01728-7","url":null,"abstract":"<p><i>Plasmopara viticola</i> (Berk. &amp; M. A. Curtis) Berl. &amp; De Toni, the causal agent of downy mildew, affects both cultivated and wild grapevine. Wild species of the genus <i>Vitis</i> L. are reliable sources of resistance to many of the diseases that affect cultivated grapevines. For this reason, it is important to study wildly growing (typical wild and feral) accessions belonging to <i>V. vinifera</i> L. The aim of this research was to study the levels of resistance to <i>P. viticola</i> of 57 Georgian wildly growing grapevine accessions by leaf disc method. The data were collected over eight years, from 2014 to 2021. Different degrees of resistance have been detected, ranging from susceptible (8.8% of accessions), low resistance (8.8% of accessions) moderate resistance (22.8%), and resistance (12.3% of accessions). Resistance of the 33,3% of the tested accessions was unstable and varied from susceptible to resistance across the years. Among resistant wildly growing grapevine genotypes, there are several accessions with a high density of the trichomes, although the data showed that the density of prostrate and erect hairs of the leaves is not correlated with the resistance trait. A large number of resistant genotypes among wildly growing grapevines suggests that in its natural habitat more resistant forms to downy mildew survives in the process of natural selection. Further studies are needed to characterize the plant-pathogen interaction and the mechanism of resistance of the wild accessions, although the resistant accessions, such as Tedotsminda 07, Tedotsminda 09, Tedotsimda 10 can be interesting for the breeding purposes in the future.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"22 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141869272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel Plasmopara viticola isolate surpasses grapevine Rpv3.1 and Rpv3.2 resistance but not Rpv12","authors":"Catarina Gouveia, Rita B. Santos, Selma Zukic, Thorsten Manthey, Rui Malhó, Andreia Figueiredo, Guenther Buchholz","doi":"10.1007/s42161-024-01707-y","DOIUrl":"https://doi.org/10.1007/s42161-024-01707-y","url":null,"abstract":"<p>Grape downy mildew, caused by the oomycete <i>Plasmopara viticola</i>, is a major threat to <i>Vitis vinifera</i> grape production. Several breeding programs rely on resistance factors (<i>Rpv</i>s) from American and Asian <i>Vitis</i> species to confer resistance to <i>P. viticola</i>. Although the use of these crossing hybrids offers a promising strategy for disease control, variants capable of overcoming host resistance are emerging. A thorough characterization of these isolates is needed to define new strategies for breeding programs. The investigation of varying aggressiveness of two <i>P. viticola</i> pathotypes was investigated. One pathotype is unable to overcome <i>Rpv3.1</i> based resistance and another pathotype was isolated from a grapevine carrying the <i>Rpv3.1</i> locus. Their aggressiveness was evaluated on five grapevine genotypes harboring distinct combinations of Rpv loci. Macroscopic and microscopic development of the pathogen was characterised throughout its colonization of grapevine leaves. Our findings demonstrate that the <i>P. viticola</i> isolate NW-10/16 is not only capable of overcoming <i>Rpv3</i>-based resistance but also exhibited a faster growth, increased sporulation, and a denser mycelial colonization within the plant mesophyll when compared with the avRpv3 + isolate in susceptible plants. The identification and characterization of new, more aggressive, <i>P. viticola</i> pathotypes is crucial to promote durable management of the resistant grapevine hybrids and to mitigate the threat of downy mildew.</p>","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"74 1","pages":""},"PeriodicalIF":2.2,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141869315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}